The aviation and oil and gas industries have shown themselves to be quite consolidated in recent years,which corroborates the growing demand for operational excellence from the main players.Therefore,in the face of an...The aviation and oil and gas industries have shown themselves to be quite consolidated in recent years,which corroborates the growing demand for operational excellence from the main players.Therefore,in the face of an increasingly competitive and fierce market,cost reduction is sought in every way,as a competitive differential against competitors.The aircraft fleet composition for offshore services represents a significant logistical cost for companies.Due to that,this paper aims to order 11 aircraft,compared in 13 different selection criteria,by the use of two multi-criteria decision analysis methods:AHP-TOPSIS-2N and THOR 2.In addition,we will propose a new method(Gaussian AHP–TOPSIS-2N)through the association of the Gaussian AHP with the AHP-TOPSIS-2N.The results showed that the aircrafts AW139,H175 and H215 obtained the best performances and the aircrafts S76,H145 and H225 had the worst performances among the alternatives of the study.展开更多
为确定H9N2猪流感病毒(H9N2-SIV)通过瞬时受体电位通道M2(TRPM2)介导肺微血管上皮细胞(PMVEC)铁死亡的分子机制,使用H9N2-SIV接种PMVEC,构建TRPM2-siRNA质粒并转染细胞。用透射电镜观察细胞超微结构,用荧光探针法检测活性氧(ROS)、Ca^(...为确定H9N2猪流感病毒(H9N2-SIV)通过瞬时受体电位通道M2(TRPM2)介导肺微血管上皮细胞(PMVEC)铁死亡的分子机制,使用H9N2-SIV接种PMVEC,构建TRPM2-siRNA质粒并转染细胞。用透射电镜观察细胞超微结构,用荧光探针法检测活性氧(ROS)、Ca^(2+)和Fe^(2+);用生化试剂盒检测丙二醛(MDA)和谷胱甘肽(GSH)含量,并通过荧光定量PCR和Western-blot检测葡萄糖调节蛋白78(GRP78)、TRPM2、蛋白激酶R样内质网激酶(PERK)、活化转录因子4(ATF4)、阳离子转运调控样蛋白1(CHAC1)、谷胱甘肽过氧化物酶4(GPX4)的m RNA和蛋白表达水平。结果显示,H9N2-SIV感染可诱导细胞铁死亡,敲低TRPM2可以减少细胞内ROS水平,降低Ca^(2+)、Fe^(2+)及MDA含量,GSH水平明显增加;此外,GRP78、PERK、ATF4、CHAC1 m RNA和蛋白表达水平下调,GPX4的m RNA和蛋白表达水平上调。结果表明,H9N2-SIV感染可诱导细胞铁死亡,其可通过激活TRPM2使Ca^(2+)内流增多,进而激活PERK/ATF4/CHAC1信号通路,加速GSH耗竭,抑制GPX4的活性,促进细胞铁死亡。展开更多
文摘The aviation and oil and gas industries have shown themselves to be quite consolidated in recent years,which corroborates the growing demand for operational excellence from the main players.Therefore,in the face of an increasingly competitive and fierce market,cost reduction is sought in every way,as a competitive differential against competitors.The aircraft fleet composition for offshore services represents a significant logistical cost for companies.Due to that,this paper aims to order 11 aircraft,compared in 13 different selection criteria,by the use of two multi-criteria decision analysis methods:AHP-TOPSIS-2N and THOR 2.In addition,we will propose a new method(Gaussian AHP–TOPSIS-2N)through the association of the Gaussian AHP with the AHP-TOPSIS-2N.The results showed that the aircrafts AW139,H175 and H215 obtained the best performances and the aircrafts S76,H145 and H225 had the worst performances among the alternatives of the study.
文摘为确定H9N2猪流感病毒(H9N2-SIV)通过瞬时受体电位通道M2(TRPM2)介导肺微血管上皮细胞(PMVEC)铁死亡的分子机制,使用H9N2-SIV接种PMVEC,构建TRPM2-siRNA质粒并转染细胞。用透射电镜观察细胞超微结构,用荧光探针法检测活性氧(ROS)、Ca^(2+)和Fe^(2+);用生化试剂盒检测丙二醛(MDA)和谷胱甘肽(GSH)含量,并通过荧光定量PCR和Western-blot检测葡萄糖调节蛋白78(GRP78)、TRPM2、蛋白激酶R样内质网激酶(PERK)、活化转录因子4(ATF4)、阳离子转运调控样蛋白1(CHAC1)、谷胱甘肽过氧化物酶4(GPX4)的m RNA和蛋白表达水平。结果显示,H9N2-SIV感染可诱导细胞铁死亡,敲低TRPM2可以减少细胞内ROS水平,降低Ca^(2+)、Fe^(2+)及MDA含量,GSH水平明显增加;此外,GRP78、PERK、ATF4、CHAC1 m RNA和蛋白表达水平下调,GPX4的m RNA和蛋白表达水平上调。结果表明,H9N2-SIV感染可诱导细胞铁死亡,其可通过激活TRPM2使Ca^(2+)内流增多,进而激活PERK/ATF4/CHAC1信号通路,加速GSH耗竭,抑制GPX4的活性,促进细胞铁死亡。
