采用扫描电镜、X射线衍射分析等手段 ,研究了正火处理对Cu Al Be B合金组织、相结构及阻尼和力学性能的影响 ,并原位观察拉伸应力作用下裂纹的萌生和扩展过程。研究结果表明 ,Cu Al Be B合金具有较高的阻尼性能 (S D C =17%～ 19% ) ,...采用扫描电镜、X射线衍射分析等手段 ,研究了正火处理对Cu Al Be B合金组织、相结构及阻尼和力学性能的影响 ,并原位观察拉伸应力作用下裂纹的萌生和扩展过程。研究结果表明 ,Cu Al Be B合金具有较高的阻尼性能 (S D C =17%～ 19% ) ,正火处理可抑制α相的析出 ,并减少γ1′的混杂程度 ,从而进一步提高阻尼能力。各向弹性异性和相变应变差引起的晶界集中应力 ,导致Cu A1 Be B合金在拉伸条件下发生晶间断裂。正火处理不但纯净晶界 ,形成马氏体共格晶界 ,而且减小晶内γ1′马氏体与 β1′马氏体的混合程度 ,有利于减少晶界应力集中和可能萌生的晶界裂纹源 ,从而有效改善合金的塑性和强度。展开更多
BACKGROUND Heterogeneous ribonucleoprotein A1(hnRNPA1)has been reported to enhance the Warburg effect and promote colon cancer(CC)cell proliferation,but the role and mechanism of the miR-490-3p/hnRNPA1-b/PKM2 axis in ...BACKGROUND Heterogeneous ribonucleoprotein A1(hnRNPA1)has been reported to enhance the Warburg effect and promote colon cancer(CC)cell proliferation,but the role and mechanism of the miR-490-3p/hnRNPA1-b/PKM2 axis in CC have not yet been elucidated.AIM To investigate the role and mechanism of a novel miR-490-3p/hnRNPA1-b/PKM2 axis in enhancing the Warburg effect and promoting CC cell proliferation through the PI3K/AKT pathway.METHODS Paraffin-embedded pathological sections from 220 CC patients were collected and subjected to immunohistochemical analysis to determine the expression of hnRNPA1-b.The relationship between the expression values and the clinicopathological features of the patients was investigated.Differences in mRNA expression were analyzed using quantitative real-time polymerase chain reaction,while differences in protein expression were analyzed using western blot.Cell proliferation was evaluated using the cell counting kit-8 and 5-ethynyl-2’-deoxyuridine assays,and cell cycle and apoptosis were detected using flow cytometric assays.The targeted binding of miR-490-3p to hnRNPA1-b was validated using a dual luciferase reporter assay.The Warburg effect was evaluated by glucose uptake and lactic acid production assays.RESULTS The expression of hnRNPA1-b was significantly increased in CC tissues and cells compared to normal controls(P<0.05).Immunohistochemical results demonstrated significant variations in the expression of the hnRNPA1-b antigen in different stages of CC,including stage I,II-III,and IV.Furthermore,the clinicopathologic characterization revealed a significant correlation between hnRNPA1-b expression and clinical stage as well as T classification.HnRNPA1-b was found to enhance the Warburg effect through the PI3K/AKT pathway,thereby promoting proliferation of HCT116 and SW620 cells.However,the proliferation of HCT116 and SW620 cells was inhibited when miR-490-3p targeted and bound to hnRNPA1-b,effectively blocking the Warburg effect.CONCLUSION These findings suggest that the novel miR-490-3p/hnRNPA1-b/PKM2 axis could provide a new strategy for the diagnosis and treatment of CC.展开更多
文摘采用扫描电镜、X射线衍射分析等手段 ,研究了正火处理对Cu Al Be B合金组织、相结构及阻尼和力学性能的影响 ,并原位观察拉伸应力作用下裂纹的萌生和扩展过程。研究结果表明 ,Cu Al Be B合金具有较高的阻尼性能 (S D C =17%～ 19% ) ,正火处理可抑制α相的析出 ,并减少γ1′的混杂程度 ,从而进一步提高阻尼能力。各向弹性异性和相变应变差引起的晶界集中应力 ,导致Cu A1 Be B合金在拉伸条件下发生晶间断裂。正火处理不但纯净晶界 ,形成马氏体共格晶界 ,而且减小晶内γ1′马氏体与 β1′马氏体的混合程度 ,有利于减少晶界应力集中和可能萌生的晶界裂纹源 ,从而有效改善合金的塑性和强度。
基金Supported by the National Natural Science Foundation of China,No.82160405Jiangxi Provincial Natural Science Foundation,No.20232BAB206131,No.20212ACB206016,and No.20224BAB206114+1 种基金Jiangxi Provincial Health Commission Project,No.202310887the Development Fund of Jiangxi Cancer Hospital,No.2021J10.
文摘BACKGROUND Heterogeneous ribonucleoprotein A1(hnRNPA1)has been reported to enhance the Warburg effect and promote colon cancer(CC)cell proliferation,but the role and mechanism of the miR-490-3p/hnRNPA1-b/PKM2 axis in CC have not yet been elucidated.AIM To investigate the role and mechanism of a novel miR-490-3p/hnRNPA1-b/PKM2 axis in enhancing the Warburg effect and promoting CC cell proliferation through the PI3K/AKT pathway.METHODS Paraffin-embedded pathological sections from 220 CC patients were collected and subjected to immunohistochemical analysis to determine the expression of hnRNPA1-b.The relationship between the expression values and the clinicopathological features of the patients was investigated.Differences in mRNA expression were analyzed using quantitative real-time polymerase chain reaction,while differences in protein expression were analyzed using western blot.Cell proliferation was evaluated using the cell counting kit-8 and 5-ethynyl-2’-deoxyuridine assays,and cell cycle and apoptosis were detected using flow cytometric assays.The targeted binding of miR-490-3p to hnRNPA1-b was validated using a dual luciferase reporter assay.The Warburg effect was evaluated by glucose uptake and lactic acid production assays.RESULTS The expression of hnRNPA1-b was significantly increased in CC tissues and cells compared to normal controls(P<0.05).Immunohistochemical results demonstrated significant variations in the expression of the hnRNPA1-b antigen in different stages of CC,including stage I,II-III,and IV.Furthermore,the clinicopathologic characterization revealed a significant correlation between hnRNPA1-b expression and clinical stage as well as T classification.HnRNPA1-b was found to enhance the Warburg effect through the PI3K/AKT pathway,thereby promoting proliferation of HCT116 and SW620 cells.However,the proliferation of HCT116 and SW620 cells was inhibited when miR-490-3p targeted and bound to hnRNPA1-b,effectively blocking the Warburg effect.CONCLUSION These findings suggest that the novel miR-490-3p/hnRNPA1-b/PKM2 axis could provide a new strategy for the diagnosis and treatment of CC.
