Random amplified polymorphic DNA(RAPD) technique was employed to detect the DNA polymorphism in 30 individuals of natural population of Pleuronectes yokohamae.20 arbitrary primers selected from 48 primers were used an...Random amplified polymorphic DNA(RAPD) technique was employed to detect the DNA polymorphism in 30 individuals of natural population of Pleuronectes yokohamae.20 arbitrary primers selected from 48 primers were used and 148 clear,repeatable RAPD bands were generated in which 94 bands were polymorphic.All the primers are polymorphic primers.Each primer used for the detection could produce 4-13 molecular markers with the average of 7.4.DNA fingerprints vary in length from 200-2 000 bp.The percentage of polymorphic fragments was 63.51%,while the index of Shannon diversity was 0.2824 and the index of gene diversity was 0.1991.Every individual had genetic difference and the genetic distance between individuals was from 0.0148 to 0.1963.Comparing the genetic diversity of Pleuronectes yokohamaes with that of other fishes,the experiment revealed that the genetic diversity of Pleuronectes yokohamae was at middle level.This result will give data for the future study of genetic diversity in Pleuronectes yokohamae.展开更多
【目的】探讨长肋日月贝幼虫发育的适宜环境因子,为其规模化繁育及种质资源保护提供参考依据。【方法】分别进行不同海水盐度和p H对长肋日月贝直线铰合幼虫存活及生长的影响试验,采用两点法与回归分析法进行长肋明贝幼虫的生存和生长...【目的】探讨长肋日月贝幼虫发育的适宜环境因子,为其规模化繁育及种质资源保护提供参考依据。【方法】分别进行不同海水盐度和p H对长肋日月贝直线铰合幼虫存活及生长的影响试验,采用两点法与回归分析法进行长肋明贝幼虫的生存和生长适宜盐度及生存和生长适宜p H界定。【结果】盐度与p H对长肋日月贝直线铰合幼虫的存活及生长有显著影响（P〈0.05）。长肋日月贝直线铰合幼虫的生存适宜盐度为23.20‰～38.02‰,最适盐度为25.00‰～35.00‰;生长适宜盐度为22.53‰～37.99‰,最适盐度为25.00‰～35.00‰。长肋日月贝直线铰合幼虫的生存适宜p H6.6～8.8,最适p H 7.0～8.5;生长适宜p H 6.3～8.6,最适p H 7.5～8.5。【结论】长肋日月贝直线铰合幼虫耐低盐能力较弱,其育苗场选址以盐度变化幅度在25.00‰～35.00‰的沿岸为宜,尽量将盐度维持在其存活及生长最适范围内,并防止育苗期间海水盐度与p H骤降。展开更多
文摘Random amplified polymorphic DNA(RAPD) technique was employed to detect the DNA polymorphism in 30 individuals of natural population of Pleuronectes yokohamae.20 arbitrary primers selected from 48 primers were used and 148 clear,repeatable RAPD bands were generated in which 94 bands were polymorphic.All the primers are polymorphic primers.Each primer used for the detection could produce 4-13 molecular markers with the average of 7.4.DNA fingerprints vary in length from 200-2 000 bp.The percentage of polymorphic fragments was 63.51%,while the index of Shannon diversity was 0.2824 and the index of gene diversity was 0.1991.Every individual had genetic difference and the genetic distance between individuals was from 0.0148 to 0.1963.Comparing the genetic diversity of Pleuronectes yokohamaes with that of other fishes,the experiment revealed that the genetic diversity of Pleuronectes yokohamae was at middle level.This result will give data for the future study of genetic diversity in Pleuronectes yokohamae.
文摘【目的】探讨长肋日月贝幼虫发育的适宜环境因子,为其规模化繁育及种质资源保护提供参考依据。【方法】分别进行不同海水盐度和p H对长肋日月贝直线铰合幼虫存活及生长的影响试验,采用两点法与回归分析法进行长肋明贝幼虫的生存和生长适宜盐度及生存和生长适宜p H界定。【结果】盐度与p H对长肋日月贝直线铰合幼虫的存活及生长有显著影响（P〈0.05）。长肋日月贝直线铰合幼虫的生存适宜盐度为23.20‰～38.02‰,最适盐度为25.00‰～35.00‰;生长适宜盐度为22.53‰～37.99‰,最适盐度为25.00‰～35.00‰。长肋日月贝直线铰合幼虫的生存适宜p H6.6～8.8,最适p H 7.0～8.5;生长适宜p H 6.3～8.6,最适p H 7.5～8.5。【结论】长肋日月贝直线铰合幼虫耐低盐能力较弱,其育苗场选址以盐度变化幅度在25.00‰～35.00‰的沿岸为宜,尽量将盐度维持在其存活及生长最适范围内,并防止育苗期间海水盐度与p H骤降。
