The title compound 9S,9aS-neotuberostemonine （1） was isolated from the 95%ethanol extract of the roots of Stemona tuberosa. The crystal structure of 1, C22H33NO4, wasdetermined by single-crystal X-ray diffraction an...The title compound 9S,9aS-neotuberostemonine （1） was isolated from the 95%ethanol extract of the roots of Stemona tuberosa. The crystal structure of 1, C22H33NO4, wasdetermined by single-crystal X-ray diffraction analysis. The crystal belongs to orthorhombicsystem, space group P212121, with a-9.0115（11）, b = 10.612（4）, c = 22.074（3） A, V= 2110.9（8）S^3, Z= 4, Mr= 375.49, Dc= 1.182 g/cm3, λ= 0.71079 A,μ= 0.080 cm^-1, F（000） = 816, S = 1.019,R = 0.0579 and wR = 0.1358. A total of 3109 unique reflections were collected, of which 2902were observed （I〉 2σ（I））. The absolute configuration of 1 could be assigned by referring to theconserved configuration of the methyl groups at C（13） and C（20）. In the solid state, the moleculeswere linked into a chain along the a-axis through weak hydrogen bond C（ll）-H（11A）…O（2）.Compound 1 shows significant inhibition of cough by 24%, 44% and 65% at doses of 50, 100 and150 mg/kg, respectively.展开更多
动物模型是将治疗方法从实验室转化到临床应用的重要工具。随着基因编辑技术的不断发展,基于成簇规则间隔短回文重复序列/CRISPR相关蛋白9(clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9,CRI...动物模型是将治疗方法从实验室转化到临床应用的重要工具。随着基因编辑技术的不断发展,基于成簇规则间隔短回文重复序列/CRISPR相关蛋白9(clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9,CRISPR/Cas9)构建的动物模型为研究发病机制、开发新的治疗方法以及评估药物疗效提供了新的可能。论文简述了CRISPR/Cas9相关技术及原理,总结了基因编辑动物模型在人类和动物转化医学研究中的应用,探讨了CRISPR/Cas9应用过程中面临的挑战和解决策略,以期促进基因编辑技术在动物模型构建中的应用。展开更多
基金supported by Guangdong Key Scientific Project(2013A022100029)Zhongshan Scientific Scheme(2017B1134)
文摘The title compound 9S,9aS-neotuberostemonine （1） was isolated from the 95%ethanol extract of the roots of Stemona tuberosa. The crystal structure of 1, C22H33NO4, wasdetermined by single-crystal X-ray diffraction analysis. The crystal belongs to orthorhombicsystem, space group P212121, with a-9.0115（11）, b = 10.612（4）, c = 22.074（3） A, V= 2110.9（8）S^3, Z= 4, Mr= 375.49, Dc= 1.182 g/cm3, λ= 0.71079 A,μ= 0.080 cm^-1, F（000） = 816, S = 1.019,R = 0.0579 and wR = 0.1358. A total of 3109 unique reflections were collected, of which 2902were observed （I〉 2σ（I））. The absolute configuration of 1 could be assigned by referring to theconserved configuration of the methyl groups at C（13） and C（20）. In the solid state, the moleculeswere linked into a chain along the a-axis through weak hydrogen bond C（ll）-H（11A）…O（2）.Compound 1 shows significant inhibition of cough by 24%, 44% and 65% at doses of 50, 100 and150 mg/kg, respectively.
文摘动物模型是将治疗方法从实验室转化到临床应用的重要工具。随着基因编辑技术的不断发展,基于成簇规则间隔短回文重复序列/CRISPR相关蛋白9(clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9,CRISPR/Cas9)构建的动物模型为研究发病机制、开发新的治疗方法以及评估药物疗效提供了新的可能。论文简述了CRISPR/Cas9相关技术及原理,总结了基因编辑动物模型在人类和动物转化医学研究中的应用,探讨了CRISPR/Cas9应用过程中面临的挑战和解决策略,以期促进基因编辑技术在动物模型构建中的应用。
