Cellular asymmetry,which represents a fundamental characteristic of cell polarity,is prominently illustrated by the apical-basal localization of PINFORMED(PIN)auxin efflux carriers in Arabidopsis thaliana.Although the...Cellular asymmetry,which represents a fundamental characteristic of cell polarity,is prominently illustrated by the apical-basal localization of PINFORMED(PIN)auxin efflux carriers in Arabidopsis thaliana.Although the maintenance of PIN polarity at the plasma membrane(PM)relies on endomembrane trafficking,the pivotal factors responsible for recruiting PIN proteins to the PM remain largely unknown.In this study,we discover that EXO70G1displays a polarized distribution at the PM in root cells.Acting as a putative subunit of the exocyst complex,which mediates the tethering of exocytic vesicles to the PM,EXO70G1 exhibits continuous recycling foci at the PM,and its dynamic behavior is akin to that of SEC6 and SEC8.Disruption of EXO70G1 and its homolog EXO70G2 in Arabidopsis reduces auxin accumulation and primary root length.Importantly,the recycling of PIN2 from the brefeldin A(BFA)compartment to the PM is compromised,and the abundance of PIN2 at the PM is reduced in the exo70G1 exo70G2 backgrounds.Interestingly,live-cell imaging reveals that the polarity of EXO70G1 is established during cytokinesis,prior to that of PIN2,and is maintained throughout the subsequent phases of cell elongation and differentiation.When the lipid raft was disturbed,the accumulation of EXO70G1 at the PM decreased.Our findings highlight the crucial role of EXO70G1 in root development by providing positional cues that facilitate the recycling efficiency of PIN2 to the PM.展开更多
AIM:To investigate the association between anti-DFS70 antibody positivity and ocular parameters,specifically,the choroidal vascularity index(CVI)and other optical coherence tomography(OCT)metrics,in a healthy populati...AIM:To investigate the association between anti-DFS70 antibody positivity and ocular parameters,specifically,the choroidal vascularity index(CVI)and other optical coherence tomography(OCT)metrics,in a healthy population.METHODS:This age-and sex-matched case-control study enrolled 84 healthy individuals with positive anti-DFS70 antibody findings and 84 healthy negative controls.All participants underwent detailed ophthalmological examinations,including biometry and OCT imaging.Anti-DFS70 positivity was determined by indirect immunofluorescence and scored semi-quantitatively(1+to 3+).CVI was calculated from OCT images using a standardized protocol with Image J software.Statistical analyses,including Student’s t-test,Mann-Whitney U test,Spearman correlation,and logistic regression,were used to compare groups and identify predictive factors.RESULTS:The individuals who tested positive and negative for anti-DFS70 included in the study were matched for age(median age=47y)and sex(F:M=7:1).CVI was significantly lower in the anti-DFS70-positive group compared to the negative group.A higher anti-DFS70 antibody titer was significantly associated with decreased subfoveal and nasal choroidal thickness(P=0.016 and P=0.014,respectively).In univariate regression analysis,CVI was the only significant predictor of anti-DFS70 positivity[odds ratio(OR)=0.02,P=0.025].Multivariate analysis revealed a positive correlation between macular thinning outside the subfoveal area and anti-DFS70 status(P<0.05).CONCLUSION:Our study demonstrates a novel association between anti-DFS70 antibody positivity and reduced choroidal vascularity in healthy individuals.These findings suggest that anti-DFS70 antibodies may be associated with subtle choroidal vascular changes detectable by OCT,even in asymptomatic individuals.Further longitudinal research is warranted to clarify the underlying mechanisms and long-term clinical significance of these ocular changes.展开更多
The microstructural evolution and mechanical properties of a vacuum electron beam welded aerospace 5B70 aluminum alloy joint were studied.Quantitative analyses of the phase composition,microstructural evolution,grain ...The microstructural evolution and mechanical properties of a vacuum electron beam welded aerospace 5B70 aluminum alloy joint were studied.Quantitative analyses of the phase composition,microstructural evolution,grain size,grain boundary density,and texture changes were performed by X-ray diffraction,scanning electron microscopy,and electron backscatter diffraction.The fusion zone(FZ)comprises equiaxed cellular crystals,and a fine~20μm-thick crystal layer forms in the transition zone(TZ)between the FZ and heat affected zone(HAZ).The HAZ closely resembles the base material(BM),retaining the original rolling microstructure.Mechanical property testing shows that the fine-grained layer in the TZ exhibits the highest nanohardness,with the FZ corresponding to the lowest microhardness.The welded-joint sample has lower yield strength,ultimate tensile strength,and elongation after fracture than the BM.These reductions of mechanical properties are primarily influenced by the grain size and distribution of the precipitated phases.展开更多
基金supported by National Natural Science Foundation of China(31571467)Shandong Province Natural Science Foundation(ZR2021MC141)。
文摘Cellular asymmetry,which represents a fundamental characteristic of cell polarity,is prominently illustrated by the apical-basal localization of PINFORMED(PIN)auxin efflux carriers in Arabidopsis thaliana.Although the maintenance of PIN polarity at the plasma membrane(PM)relies on endomembrane trafficking,the pivotal factors responsible for recruiting PIN proteins to the PM remain largely unknown.In this study,we discover that EXO70G1displays a polarized distribution at the PM in root cells.Acting as a putative subunit of the exocyst complex,which mediates the tethering of exocytic vesicles to the PM,EXO70G1 exhibits continuous recycling foci at the PM,and its dynamic behavior is akin to that of SEC6 and SEC8.Disruption of EXO70G1 and its homolog EXO70G2 in Arabidopsis reduces auxin accumulation and primary root length.Importantly,the recycling of PIN2 from the brefeldin A(BFA)compartment to the PM is compromised,and the abundance of PIN2 at the PM is reduced in the exo70G1 exo70G2 backgrounds.Interestingly,live-cell imaging reveals that the polarity of EXO70G1 is established during cytokinesis,prior to that of PIN2,and is maintained throughout the subsequent phases of cell elongation and differentiation.When the lipid raft was disturbed,the accumulation of EXO70G1 at the PM decreased.Our findings highlight the crucial role of EXO70G1 in root development by providing positional cues that facilitate the recycling efficiency of PIN2 to the PM.
文摘AIM:To investigate the association between anti-DFS70 antibody positivity and ocular parameters,specifically,the choroidal vascularity index(CVI)and other optical coherence tomography(OCT)metrics,in a healthy population.METHODS:This age-and sex-matched case-control study enrolled 84 healthy individuals with positive anti-DFS70 antibody findings and 84 healthy negative controls.All participants underwent detailed ophthalmological examinations,including biometry and OCT imaging.Anti-DFS70 positivity was determined by indirect immunofluorescence and scored semi-quantitatively(1+to 3+).CVI was calculated from OCT images using a standardized protocol with Image J software.Statistical analyses,including Student’s t-test,Mann-Whitney U test,Spearman correlation,and logistic regression,were used to compare groups and identify predictive factors.RESULTS:The individuals who tested positive and negative for anti-DFS70 included in the study were matched for age(median age=47y)and sex(F:M=7:1).CVI was significantly lower in the anti-DFS70-positive group compared to the negative group.A higher anti-DFS70 antibody titer was significantly associated with decreased subfoveal and nasal choroidal thickness(P=0.016 and P=0.014,respectively).In univariate regression analysis,CVI was the only significant predictor of anti-DFS70 positivity[odds ratio(OR)=0.02,P=0.025].Multivariate analysis revealed a positive correlation between macular thinning outside the subfoveal area and anti-DFS70 status(P<0.05).CONCLUSION:Our study demonstrates a novel association between anti-DFS70 antibody positivity and reduced choroidal vascularity in healthy individuals.These findings suggest that anti-DFS70 antibodies may be associated with subtle choroidal vascular changes detectable by OCT,even in asymptomatic individuals.Further longitudinal research is warranted to clarify the underlying mechanisms and long-term clinical significance of these ocular changes.
基金supported by the National Natural Science Foundation of China(Nos.52175206,52205187,52130509)the Science and Technology Planning Project of Guizhou Province,China(No.ZK[2022]013)。
文摘The microstructural evolution and mechanical properties of a vacuum electron beam welded aerospace 5B70 aluminum alloy joint were studied.Quantitative analyses of the phase composition,microstructural evolution,grain size,grain boundary density,and texture changes were performed by X-ray diffraction,scanning electron microscopy,and electron backscatter diffraction.The fusion zone(FZ)comprises equiaxed cellular crystals,and a fine~20μm-thick crystal layer forms in the transition zone(TZ)between the FZ and heat affected zone(HAZ).The HAZ closely resembles the base material(BM),retaining the original rolling microstructure.Mechanical property testing shows that the fine-grained layer in the TZ exhibits the highest nanohardness,with the FZ corresponding to the lowest microhardness.The welded-joint sample has lower yield strength,ultimate tensile strength,and elongation after fracture than the BM.These reductions of mechanical properties are primarily influenced by the grain size and distribution of the precipitated phases.
