Objectives:Colorectal cancer(CRC)is a major global health burden,and Urolithin A(Uro-A)has emerged as a promising anticancer agent.This systematic review aims to synthesize current in vitro evidence on the anticancer ...Objectives:Colorectal cancer(CRC)is a major global health burden,and Urolithin A(Uro-A)has emerged as a promising anticancer agent.This systematic review aims to synthesize current in vitro evidence on the anticancer effects of Uro-A in CRC,highlighting effective concentration ranges,exposure times,relevant outcomes,and underlying molecular mechanisms.Methods:Following PRISMA 2020 guidelines,a systematic search was conducted in PubMed,Scopus,and Web of Science using the following strategy:(colorectal cancer)AND(urolithin a)OR(3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one).Eligibility criteria were defined by the PICO framework:(P)in vitro CRC cell models;(I)Uro-A alone or combined treatments;(C)No intervention,vehicle or other treatments;(O)Relevant anticancer outcomes of Uro-A in CRC.Only original,full-text,in vitro studies in English were included.Risk of bias was assessed using ToxRTool.A qualitative synthesis was performed due to the heterogeneity of the included studies.Results:Fifteen studies met inclusion criteria,involving CRC cell lines(Caco-2,HCT-116,HT-29,SW480,SW620)and normal colon fibroblasts(CCD18-Co).Uro-A inhibited CRC cell proliferation,clonogenic growth,cancer stem cells properties,migration,and invasion,and induced cell cycle arrest,apoptosis,autophagy,and senescence,through modulation of key signaling pathways and proteins.Co-treatments with conventional chemotherapeutics and microbiota-derived metabolites showed additive or synergistic effects.Discussion:The findings support UroA’s potential as a preventive or adjuvant agent in CRC treatment.However,preclinical nature of the evidence and methodological heterogeneity hinder clinical extrapolation to in vivo contexts.Human clinical trials are necessary to overcome these limitations.Other:This review was registered in PROSPERO(CRD420251070874)and supported by FCT/MCTES UIDP/05608/2020 and UIDB/05608/2020.Institutional.展开更多
Background:The traditional Sox10 ^(Dom/+)mouse breeding strategy is costly and timeconsuming,so this study aims to optimize the breeding method and improve the scientific research efficiency.Methods:We select the offs...Background:The traditional Sox10 ^(Dom/+)mouse breeding strategy is costly and timeconsuming,so this study aims to optimize the breeding method and improve the scientific research efficiency.Methods:We select the offspring from mating B6C3Fe Sox10 ^(Dom/+)male mice with C57BL/6J female mice,and name the progeny B6C3Fe-g.Further,conduct separate self-breeding for both the B6C3Fe and B6C3Fe-g strains,adhering to the principle of pairing mutants with non-mutants.By comparing the number of offspring,survival rates,and the phenotype of aganglionosis in the colon,a comprehensive evaluation of their breeding capacity and phenotypic stability is conducted.Results:Sanger sequencing results show that the mutation sites of B6C3Fe and B6C3Fe-g mice are consistent.After fluorescent staining of intestinal nerves,it was found that the heterozygous mice of the two strains had neuronal deletion in the distal colon,and this pathological phenotype was consistent with the pathological features of the diseased colon of Hirschsprung disease(HSCR).However,compared with the B6C3Fe strain,the B6C3Fe-g strain has a higher number of offspring and greater survival rates.Conclusions:The breeding strategy of the B6C3Fe-g strain ensures genetic and phenotypic stability,while improving reproductive efficiency,and is an ideal scheme for breeding Sox10 ^(Dom/+)mice.展开更多
Using Hartogs’fundamental theorem for analytic functions in several complex variables and q-partial differential equations,we establish a multiple q-exponential differential formula for analytic functions in several ...Using Hartogs’fundamental theorem for analytic functions in several complex variables and q-partial differential equations,we establish a multiple q-exponential differential formula for analytic functions in several variables.With this identity,we give new proofs of a variety of important classical formulas including Bailey’s 6ψ6 series summation formula and the Atakishiyev integral.A new transformation formula for a double q-series with several interesting special cases is given.A new transformation formula for a 3ψ3 series is proved.展开更多
基金supported by FCT/MCTES UIDP/05608/2020(https://doi.org/10.54499/UIDP/05608/2020,accessed on 01 July 2025)UIDB/05608/2020(https://doi.org/10.54499/UIDB/05608/2020,accessed on 01 July 2025).Institutional.
文摘Objectives:Colorectal cancer(CRC)is a major global health burden,and Urolithin A(Uro-A)has emerged as a promising anticancer agent.This systematic review aims to synthesize current in vitro evidence on the anticancer effects of Uro-A in CRC,highlighting effective concentration ranges,exposure times,relevant outcomes,and underlying molecular mechanisms.Methods:Following PRISMA 2020 guidelines,a systematic search was conducted in PubMed,Scopus,and Web of Science using the following strategy:(colorectal cancer)AND(urolithin a)OR(3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one).Eligibility criteria were defined by the PICO framework:(P)in vitro CRC cell models;(I)Uro-A alone or combined treatments;(C)No intervention,vehicle or other treatments;(O)Relevant anticancer outcomes of Uro-A in CRC.Only original,full-text,in vitro studies in English were included.Risk of bias was assessed using ToxRTool.A qualitative synthesis was performed due to the heterogeneity of the included studies.Results:Fifteen studies met inclusion criteria,involving CRC cell lines(Caco-2,HCT-116,HT-29,SW480,SW620)and normal colon fibroblasts(CCD18-Co).Uro-A inhibited CRC cell proliferation,clonogenic growth,cancer stem cells properties,migration,and invasion,and induced cell cycle arrest,apoptosis,autophagy,and senescence,through modulation of key signaling pathways and proteins.Co-treatments with conventional chemotherapeutics and microbiota-derived metabolites showed additive or synergistic effects.Discussion:The findings support UroA’s potential as a preventive or adjuvant agent in CRC treatment.However,preclinical nature of the evidence and methodological heterogeneity hinder clinical extrapolation to in vivo contexts.Human clinical trials are necessary to overcome these limitations.Other:This review was registered in PROSPERO(CRD420251070874)and supported by FCT/MCTES UIDP/05608/2020 and UIDB/05608/2020.Institutional.
基金Basic and Applied Basic Research Foundation of Guangxi Province of China,Grant/Award Number:2025GXNSFBA069072National Natural Science Foundation of China,Grant/Award Number:81970450,82070528,82170528,82200561,82201893,82301955,82370526 and 82560108+2 种基金Medical Scientific Research Foundation of Guangdong Province of China,Grant/Award Number:A2024374The Guangdong Basic and Applied Basic Research Foundation,Grant/Award Number:2024A03J1171,2024A03J1238 and 2024A1515013190Science and Technology Projects in Guangzhou,Grant/Award Number:202201020006。
文摘Background:The traditional Sox10 ^(Dom/+)mouse breeding strategy is costly and timeconsuming,so this study aims to optimize the breeding method and improve the scientific research efficiency.Methods:We select the offspring from mating B6C3Fe Sox10 ^(Dom/+)male mice with C57BL/6J female mice,and name the progeny B6C3Fe-g.Further,conduct separate self-breeding for both the B6C3Fe and B6C3Fe-g strains,adhering to the principle of pairing mutants with non-mutants.By comparing the number of offspring,survival rates,and the phenotype of aganglionosis in the colon,a comprehensive evaluation of their breeding capacity and phenotypic stability is conducted.Results:Sanger sequencing results show that the mutation sites of B6C3Fe and B6C3Fe-g mice are consistent.After fluorescent staining of intestinal nerves,it was found that the heterozygous mice of the two strains had neuronal deletion in the distal colon,and this pathological phenotype was consistent with the pathological features of the diseased colon of Hirschsprung disease(HSCR).However,compared with the B6C3Fe strain,the B6C3Fe-g strain has a higher number of offspring and greater survival rates.Conclusions:The breeding strategy of the B6C3Fe-g strain ensures genetic and phenotypic stability,while improving reproductive efficiency,and is an ideal scheme for breeding Sox10 ^(Dom/+)mice.
基金supported by the National Natural Science Foundation of China(11971173)the Science and Technology Commission of Shanghai Municipality(22DZ2229014).
文摘Using Hartogs’fundamental theorem for analytic functions in several complex variables and q-partial differential equations,we establish a multiple q-exponential differential formula for analytic functions in several variables.With this identity,we give new proofs of a variety of important classical formulas including Bailey’s 6ψ6 series summation formula and the Atakishiyev integral.A new transformation formula for a double q-series with several interesting special cases is given.A new transformation formula for a 3ψ3 series is proved.
