The 28 ku membrane protein is usually over-expressl m human bnasl bmast cancer and other tumor cells. licould be a larget for tumor therapy . By using genetie engineermg teehmgues.a 606 immunotoxin (sefv606-PE40) was ...The 28 ku membrane protein is usually over-expressl m human bnasl bmast cancer and other tumor cells. licould be a larget for tumor therapy . By using genetie engineermg teehmgues.a 606 immunotoxin (sefv606-PE40) was construeted by joining the 606 single-chain antibdy (SeFv606) with the truncll Pseudonwnas exotoxin A (PE40), SeFv606 contains both the heavy and light-chnia variable domams of 606 monoelonal antibody. Which speeifieally ree-ognizes the 28 ku protein. The bacterial expression level og 606 imnmmotoxin is 3.3%. about 5.5 mg ml baeterial lysate.lsing singlc-step llisTrap (Nr2 chelating) column chronnetogaphy, the reeombinant peptide was obtained with a purit of 33.2%.This baeterial espressed 606 immunotosin binuls to MDA-231 human breast-tumer ccll surfaee and kill these cells with a median lethal dosage of 92 ngnd.展开更多
Background:The traditional Sox10 ^(Dom/+)mouse breeding strategy is costly and timeconsuming,so this study aims to optimize the breeding method and improve the scientific research efficiency.Methods:We select the offs...Background:The traditional Sox10 ^(Dom/+)mouse breeding strategy is costly and timeconsuming,so this study aims to optimize the breeding method and improve the scientific research efficiency.Methods:We select the offspring from mating B6C3Fe Sox10 ^(Dom/+)male mice with C57BL/6J female mice,and name the progeny B6C3Fe-g.Further,conduct separate self-breeding for both the B6C3Fe and B6C3Fe-g strains,adhering to the principle of pairing mutants with non-mutants.By comparing the number of offspring,survival rates,and the phenotype of aganglionosis in the colon,a comprehensive evaluation of their breeding capacity and phenotypic stability is conducted.Results:Sanger sequencing results show that the mutation sites of B6C3Fe and B6C3Fe-g mice are consistent.After fluorescent staining of intestinal nerves,it was found that the heterozygous mice of the two strains had neuronal deletion in the distal colon,and this pathological phenotype was consistent with the pathological features of the diseased colon of Hirschsprung disease(HSCR).However,compared with the B6C3Fe strain,the B6C3Fe-g strain has a higher number of offspring and greater survival rates.Conclusions:The breeding strategy of the B6C3Fe-g strain ensures genetic and phenotypic stability,while improving reproductive efficiency,and is an ideal scheme for breeding Sox10 ^(Dom/+)mice.展开更多
The neutron capture cross section for^(165)Ho was measured at the backstreaming white neutron beam line(Back-n)of the China Spallation Neutron Source(CSNS)using total energy detection systems,composed of a set of four...The neutron capture cross section for^(165)Ho was measured at the backstreaming white neutron beam line(Back-n)of the China Spallation Neutron Source(CSNS)using total energy detection systems,composed of a set of four C_(6)D_(6)scintillator detectors coupled with pulse height weighting techniques.The resonance parameters were extracted using the multilevel multichannel R-matrix code SAMMY to fit the measured capture yields of the^(165)Ho(n,γ)reaction in the neutron energy range below100 eV.Subsequently,the resonance region’s capture cross sections were reconstructed based on the obtained parameters.Furthermore,the unresolved resonance average cross section of the^(165)Ho(n,γ)reaction was determined relative to that of the standard sample^(197)Au within the neutron energy range of 2 keV to 1 MeV.The experimental data were compared with the recommended nuclear data from the ENDF/B-VIII.0 library,as well as with results of calculations performed using the TALYS-1.9 code.The comparison revealed agreement between the measured^(165)Ho(n,γ)cross sections and these data.The present results are crucial for evaluating the^(165)Ho neutron capture cross section and thus enhance the quality of evaluated nuclear data libraries.They provide valuable guidance for nuclear theoretical models and nuclear astrophysical studies.展开更多
基金Project Supported by the Chinese Natiotal High-Tech Project
文摘The 28 ku membrane protein is usually over-expressl m human bnasl bmast cancer and other tumor cells. licould be a larget for tumor therapy . By using genetie engineermg teehmgues.a 606 immunotoxin (sefv606-PE40) was construeted by joining the 606 single-chain antibdy (SeFv606) with the truncll Pseudonwnas exotoxin A (PE40), SeFv606 contains both the heavy and light-chnia variable domams of 606 monoelonal antibody. Which speeifieally ree-ognizes the 28 ku protein. The bacterial expression level og 606 imnmmotoxin is 3.3%. about 5.5 mg ml baeterial lysate.lsing singlc-step llisTrap (Nr2 chelating) column chronnetogaphy, the reeombinant peptide was obtained with a purit of 33.2%.This baeterial espressed 606 immunotosin binuls to MDA-231 human breast-tumer ccll surfaee and kill these cells with a median lethal dosage of 92 ngnd.
基金Basic and Applied Basic Research Foundation of Guangxi Province of China,Grant/Award Number:2025GXNSFBA069072National Natural Science Foundation of China,Grant/Award Number:81970450,82070528,82170528,82200561,82201893,82301955,82370526 and 82560108+2 种基金Medical Scientific Research Foundation of Guangdong Province of China,Grant/Award Number:A2024374The Guangdong Basic and Applied Basic Research Foundation,Grant/Award Number:2024A03J1171,2024A03J1238 and 2024A1515013190Science and Technology Projects in Guangzhou,Grant/Award Number:202201020006。
文摘Background:The traditional Sox10 ^(Dom/+)mouse breeding strategy is costly and timeconsuming,so this study aims to optimize the breeding method and improve the scientific research efficiency.Methods:We select the offspring from mating B6C3Fe Sox10 ^(Dom/+)male mice with C57BL/6J female mice,and name the progeny B6C3Fe-g.Further,conduct separate self-breeding for both the B6C3Fe and B6C3Fe-g strains,adhering to the principle of pairing mutants with non-mutants.By comparing the number of offspring,survival rates,and the phenotype of aganglionosis in the colon,a comprehensive evaluation of their breeding capacity and phenotypic stability is conducted.Results:Sanger sequencing results show that the mutation sites of B6C3Fe and B6C3Fe-g mice are consistent.After fluorescent staining of intestinal nerves,it was found that the heterozygous mice of the two strains had neuronal deletion in the distal colon,and this pathological phenotype was consistent with the pathological features of the diseased colon of Hirschsprung disease(HSCR).However,compared with the B6C3Fe strain,the B6C3Fe-g strain has a higher number of offspring and greater survival rates.Conclusions:The breeding strategy of the B6C3Fe-g strain ensures genetic and phenotypic stability,while improving reproductive efficiency,and is an ideal scheme for breeding Sox10 ^(Dom/+)mice.
基金supported by the National Natural Science Foundation of China(Nos.12465024,12365018,U2032146)Inner Mongolia National Science Foundation(Nos.2024ZD23,2024FX30,2023MS01005)+1 种基金Program for Innovative Research Team in Universities of Inner Mongolia Autonomous Region(NMGIRT2217)Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region(NJYT23109)。
文摘The neutron capture cross section for^(165)Ho was measured at the backstreaming white neutron beam line(Back-n)of the China Spallation Neutron Source(CSNS)using total energy detection systems,composed of a set of four C_(6)D_(6)scintillator detectors coupled with pulse height weighting techniques.The resonance parameters were extracted using the multilevel multichannel R-matrix code SAMMY to fit the measured capture yields of the^(165)Ho(n,γ)reaction in the neutron energy range below100 eV.Subsequently,the resonance region’s capture cross sections were reconstructed based on the obtained parameters.Furthermore,the unresolved resonance average cross section of the^(165)Ho(n,γ)reaction was determined relative to that of the standard sample^(197)Au within the neutron energy range of 2 keV to 1 MeV.The experimental data were compared with the recommended nuclear data from the ENDF/B-VIII.0 library,as well as with results of calculations performed using the TALYS-1.9 code.The comparison revealed agreement between the measured^(165)Ho(n,γ)cross sections and these data.The present results are crucial for evaluating the^(165)Ho neutron capture cross section and thus enhance the quality of evaluated nuclear data libraries.They provide valuable guidance for nuclear theoretical models and nuclear astrophysical studies.
