摘要
目的 检测细胞色素c氧化酶组装因子6(COA6)在乳腺癌中的表达及临床意义,分析COA6与乳腺癌免疫浸润的相关性。方法 全转录组测序筛选差异基因,并联合TCGA数据库验证COA6的表达。免疫组化技术检测125例乳腺癌组织及癌旁组织COA6蛋白表达,分析其与临床特征的相关性。分别用qRT-PCR、Western blot检测乳腺癌细胞、组织中COA6 mRNA及蛋白表达。利用TIMER数据库分析COA6基因的高表达与免疫细胞浸润的关系。结果 乳腺癌组织中COA6的阳性表达率为(88%, 110/125),显著高于癌旁组织(7.2%, 9/125)(P<0.05),并且与肿瘤的大小和组织学分级呈正相关。在新鲜乳腺癌组织中,COA6蛋白的表达水平明显高于癌旁组织。在乳腺癌细胞系中,COA6 mRNA和蛋白表达均明显增加。COA6与辅助T细胞、NK细胞、CD8+T细胞、M1型巨噬细胞、调节性T细胞、树突细胞、记忆性CD4+T细胞在肿瘤微环境中的浸润有关。结论 COA6在乳腺癌中的表达水平升高,并且与肿瘤免疫浸润呈正相关,这为乳腺癌治疗提供了潜在的治疗靶点。
Objective To detect the expression of cytochrome c oxidase assembly factor 6(COA6)in breast cancer,study its clinical significance,and analyze the effect of COA6 on immune infiltration in breast cancer.Methods Differential genes were screened by the whole transcriptome sequencing and the expression of COA6 was explored with TCGA(The Cancer Genome Atlas Program)database.The tissues of 125 breast cancer and adjacent tissues were stained by immunohistochemistry to detect COA6 protein expression and analyze the correlation with clinical features.The COA6 mRNA and protein expression in breast cancer cells and tissues were determined by qRT-PCR and Western blot,respectively.The TIMER(Tumor Immune Estimation Resource)database was used to analyze the correlation between high COA6 gene expression and immune cell infiltration.Results The positive expression rate of COA6 in breast cancer tissues was 88%(110/125),which was higher than that of paracarinoma tissues(7.2%,9/125)(P<0.05)and was positively correlated with tumor size and histological grade.In fresh breast cancer tissues,COA6 protein expression was significantly higher than that in adjacent tissues.Both COA6 mRNA and protein expression were significantly increased in the breast cancer cell lines.COA6 was positively correlated with the infiltration of helper T cells,NK cells,CD8+T cells,M1 type macrophages,regulatory T cells,dendritic cells,and memory CD4+T cells in the tumor microenvironment.Conclusions The expression level of COA6 is increased in breast cancer and is positively correlated with tumor immune infiltration,which provides a potential therapeutic target for breast cancer treatment.
作者
金晓霞
刘玉山
胡继平
朱兴华
JIN Xiaoxia;LIU Yushan;HU Jiping;ZHU Xinghua(Department of Pathology,Tumor Hospital Affiliated to Nantong University,Nantong 226000;Department of Clinical Pathology,Medical College,Nantong University,Nantong 226000,China)
出处
《基础医学与临床》
2025年第6期755-761,共7页
Basic and Clinical Medicine
基金
南通市科技计划指令性项目(MS22022015)。
