目的 探究血清多聚嘧啶区结合蛋白1(polypyrimidine tract binding protein 1,PTBP1)、G蛋白信号调节因子2(regulator of G protein signaling 2,RGS2)、5-羟色胺(5-hydroxytryptamine, 5-HT)水平在慢性萎缩性胃炎(chronic atrophic gas...目的 探究血清多聚嘧啶区结合蛋白1(polypyrimidine tract binding protein 1,PTBP1)、G蛋白信号调节因子2(regulator of G protein signaling 2,RGS2)、5-羟色胺(5-hydroxytryptamine, 5-HT)水平在慢性萎缩性胃炎(chronic atrophic gastritis, CAG)诊断及病情严重程度评估中的价值。方法 选取2022年1月至2024年10月我院收治的143例CAG患者为研究组,根据胃固有腺体减少程度分为轻度组(n=26)、中度组(n=75)、重度组(n=42)。另纳入152名体检健康人群为对照组。采用酶联免疫吸附法(enzyme linked immunosorbent assay, ELISA)测定各组血清PTBP1、RGS2、5-HT的表达。Logistic回归分析CAG影响因素。ROC曲线分析血清PTBP1、RGS2、5-HT对CAG的诊断及病情严重程度评估价值。结果 与对照组相比,研究组血清PTBP1、RGS2、5-HT表达均升高(P<0.05)。重度组血清PTBP1、RGS2、5-HT高于轻度组、中度组(P<0.05),中度组血清PTBP1、5-HT高于轻度组(P<0.05)。PTBP1、RGS2、5-HT均为CAG发生的危险因素(P<0.05)。血清PTBP1、RGS2、5-HT联合诊断CAG的AUC为0.957,显著大于PTBP1(Z=6.160,P<0.001)、RGS2(Z=4.240,P<0.001)、5-HT(Z=3.248,P=0.001)单独诊断的AUC。PTBP1、RGS2、5-HT联合诊断重度CAG的AUC为0.973,显著大于PTBP1(Z=3.799,P<0.001)、RGS2(Z=5.018,P<0.001)单独诊断的AUC,与5-HT(Z=1.760,P=0.078)单独诊断的AUC,差异无统计学意义。结论 CAG患者血清PTBP1、RGS2、5-HT水平均升高,三者联合具有一定的CAG诊断及病情严重程度评估价值。展开更多
Objective:To investigate the relationship between Jiaotai pill(JTP),its main component berberine(BBR),and the serotonin(5-HT)system in regulating islet hormone secretion and alleviating pancreatic b-cell dysfunction d...Objective:To investigate the relationship between Jiaotai pill(JTP),its main component berberine(BBR),and the serotonin(5-HT)system in regulating islet hormone secretion and alleviating pancreatic b-cell dysfunction during type 2 diabetes mellitus(T2DM)progression.Methods:T2DM rat model was established using a high-fat diet and streptozotocin injection.JTP,BBR,and Metformin were intragastrically administered for 35 days.The analyzed indices included blood glucose,blood lipids,islet hormones,and proteins related to 5-HT synthesis,secretion,and transport.Additionally,an in vitro model of glucose injury in islet cells was established to study the effects of JTP and BBR on islet hormone secretion following tryptophan hydroxylase 1(TPH1)inhibition.Results:JTP and BBR significantly improved blood glucose and lipid levels and islet morphology in T2DM rats.Both models exhibited reduced islet 5-HT levels and impaired islet hormone secretion.However,the administration of JTP and BBR reversed these effects.Furthermore,JTP and BBR upregulated the expression of TPH1(P=.0194,P=.0413)transglutaminase 2(TGase2;P=.0492,P=.0349),serotonin transporter(SERT,P=.0090),and 5-hydroxytryptamine 1F receptor(5-HT1FR)in the islet 5-HT pathway(P=.0194).In the cell model,the regulatory effects of JTP and BBR on islet hormone levels were significantly weakened after TPH1 inhibition(P=.001),suggesting that JTP and BBR influence islet hormone secretion through the pancreatic 5-HT system.Conclusion:The islet 5-HT system is correlated with islet hormone secretion dysfunction in T2DM.JTP and BBR can improve islet hormone secretion by activating the TPH1/TGase2/SERT/5-HT1FR pathway in the islet 5-HT system in T2DM rats.展开更多
基金supported by the Shanxi Province Overseas Returnees Research Funding Project(No.2023-102)Shanxi Province Postgraduate Research Innovation Program(No.2023KY385,2023KY360,2022Y396)。
基金supported by the National Natural Science Foundation of China(81673680)the Fundamental Research Funds for the Central Public Welfare Research Institutes(YZX-202306).
文摘Objective:To investigate the relationship between Jiaotai pill(JTP),its main component berberine(BBR),and the serotonin(5-HT)system in regulating islet hormone secretion and alleviating pancreatic b-cell dysfunction during type 2 diabetes mellitus(T2DM)progression.Methods:T2DM rat model was established using a high-fat diet and streptozotocin injection.JTP,BBR,and Metformin were intragastrically administered for 35 days.The analyzed indices included blood glucose,blood lipids,islet hormones,and proteins related to 5-HT synthesis,secretion,and transport.Additionally,an in vitro model of glucose injury in islet cells was established to study the effects of JTP and BBR on islet hormone secretion following tryptophan hydroxylase 1(TPH1)inhibition.Results:JTP and BBR significantly improved blood glucose and lipid levels and islet morphology in T2DM rats.Both models exhibited reduced islet 5-HT levels and impaired islet hormone secretion.However,the administration of JTP and BBR reversed these effects.Furthermore,JTP and BBR upregulated the expression of TPH1(P=.0194,P=.0413)transglutaminase 2(TGase2;P=.0492,P=.0349),serotonin transporter(SERT,P=.0090),and 5-hydroxytryptamine 1F receptor(5-HT1FR)in the islet 5-HT pathway(P=.0194).In the cell model,the regulatory effects of JTP and BBR on islet hormone levels were significantly weakened after TPH1 inhibition(P=.001),suggesting that JTP and BBR influence islet hormone secretion through the pancreatic 5-HT system.Conclusion:The islet 5-HT system is correlated with islet hormone secretion dysfunction in T2DM.JTP and BBR can improve islet hormone secretion by activating the TPH1/TGase2/SERT/5-HT1FR pathway in the islet 5-HT system in T2DM rats.
