目的:本文旨在研究胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)D442G基因多态性与脑梗死发病的相关性。方法:选取包头医学院第一附属医院神经内科脑梗死患者180例为观察组,体检科体检人员130例为对照组。DNA试剂盒提取...目的:本文旨在研究胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)D442G基因多态性与脑梗死发病的相关性。方法:选取包头医学院第一附属医院神经内科脑梗死患者180例为观察组,体检科体检人员130例为对照组。DNA试剂盒提取外周血DNA,采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorph,PCR-RFLP)方法检测CETP D442G的基因型,分析D442G基因型与脑梗死发病的关系。结果:D442G基因位点DG基因型高密度脂蛋白水平高于GG、DD基因型,差异有统计学意义(P<0.05)。以是否发生脑梗死作为应变量,以年龄、性别、体重指数、血脂水平、糖尿病、高血压、心脏病、CETP D442G基因型及等位基因频率作为自变量,应用非条件logistic回归分析得出年龄、高血压、糖尿病、甘油三酯、胆固醇、低密度脂蛋白为脑梗死发病的独立危险因素(OR>1,P<0.05);高密度脂蛋白、DG基因型为脑梗死发病的保护因素(OR<1,P<0.05)。结论:脑梗死发病的危险因素可能为年龄、吸烟史、高血压病史、糖尿病病史、胆固醇、低密度脂蛋白、甘油三酯水平,脑梗死发病的保护因素可能为D442G基因DG基因型及高密度脂蛋白水平。展开更多
The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Opt...The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Optimal condition for immobilization was performed by adjusting tube distance to CaCI2 solution to be 4-6 cm and 7 mL/min flow rate of alginate gel. The production of propionic acid by immobilized cells in a 2 L fermentor using 1% CaCO3 and 5 N KOH to control the pH at 6.5 gave maximum propionic acid and they had consistent potential to recycle 2 rounds of fermentation and produced the total of 29.24 g/L propionic acid （15.85 ± 0.25 g/L and 13.39 ± 0.25 g/L propionic acid from Batch 1 and Batch 2 fermentation, respectively）. Compared to free-cell fermentation, propionic acid productivity increased 20% （0.083 g/h vs. 0.070 g/h） and fermentation time reduced 11% （192 h vs. 216 h） in 2 L fermentor with 40 g/L initial total sugar from whey. The fermented propionic acid as well as the commercial propionic acid from chemical process was able to inhibit the growth of the fungal tested.展开更多
文摘The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Optimal condition for immobilization was performed by adjusting tube distance to CaCI2 solution to be 4-6 cm and 7 mL/min flow rate of alginate gel. The production of propionic acid by immobilized cells in a 2 L fermentor using 1% CaCO3 and 5 N KOH to control the pH at 6.5 gave maximum propionic acid and they had consistent potential to recycle 2 rounds of fermentation and produced the total of 29.24 g/L propionic acid （15.85 ± 0.25 g/L and 13.39 ± 0.25 g/L propionic acid from Batch 1 and Batch 2 fermentation, respectively）. Compared to free-cell fermentation, propionic acid productivity increased 20% （0.083 g/h vs. 0.070 g/h） and fermentation time reduced 11% （192 h vs. 216 h） in 2 L fermentor with 40 g/L initial total sugar from whey. The fermented propionic acid as well as the commercial propionic acid from chemical process was able to inhibit the growth of the fungal tested.
