摘要
测定3T3-F442A细胞分化成脂肪细胞的过程中，细胞表达氨基脲敏感胺氧化酶（Semicarbazide-sensitiveamineoxidase，SSAO）活性的变化情况，用于SSAO病理生理学功能的研究。3T3-F442A细胞在分化的不同天数内，收集细胞，超声波破碎后制备SSAO初提物。用分光光度法研究酶活性的基础上加入一种氧化酶，即采用酶联分光光度法分别研究细胞分化不同时间所表达的SSAO活性。结果显示，体外3T3-F442A细胞在分化过程中，SSAO在细胞内的表达呈曲线上升趋势。分化前细胞内几乎检测不到SSAO的存在，分化的第3d只能检测到少量SSAO，此后，SSAO的表达呈急剧上升趋势，细胞分化后的第6～7dSSAO活性达到高峰。
In order to probe into the patho-physiologT semicarbazide-sensitive amine oxidase （ SSAO）, we determined the wariation of its activity in the days of 3T3-F442A ceils＇ differentiation into adipocytes in vitro. The differentiated 3T3-F442A ceils were collected in various days and disrupted by ultrasonication. Then we studied the SSAO activity of the collected cells by oxidase-linked spectrophotometric assay. In the course of 3T3-F442A ceils＇ differentiation, SSAO expression was shown by a curve graph. There was no SSAO detected in 3T3-F442A preadipocyte, and only a little of SSAO was detected in the cells on the 3rd day of differentiation. Since then, SSAO significantly increased and reached to a maximtml level six or seven days after the cells＇ differentiation.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2008年第5期1161-1165,共5页
Journal of Biomedical Engineering
基金
河南省医学科技攻关基金资助项目(200703008)
河南省教育厅自然科学研究基金资助项目(2008B180013)
关键词
3T3-F442A细胞
氨基脲敏感胺氧化酶
分化
酶联分光光度法
3T3-F442A ceils Semicarbazide-sensitive amine oxidase （SSAO） Differentiation Oxidaselinked spectrophotometric assay
