Passivity breakdown on 436 ferritic stainless steel(FSS) has been investigated in solutions containing different concentrations of chloride at 25?C and interpreted in terms of the point defect model(PDM). The measured...Passivity breakdown on 436 ferritic stainless steel(FSS) has been investigated in solutions containing different concentrations of chloride at 25?C and interpreted in terms of the point defect model(PDM). The measured near-normal distributions of passivity breakdown potentials for 436 FSS under experimental conditions are in good agreement with the calculated results according to the PDM. The linear dependence of breakdown potential on the square root of potential scanning rate, which was described by the PDM,provides the estimation of the critical concentration of condensed vacancies at the metal/film interface,which leads to the passivity breakdown. This value is in good agreement with that calculated from the microstructure properties of the alloy substrate and the barrier layer of the passive film. This study demonstrates the validity of the PDM in describing the passivity breakdown on 436 FSS in NaCl solutions.展开更多
目的探讨锌指蛋白ZNF436在人胃癌细胞系和组织中的相对表达,及ZNF436对胃癌细胞MKN-28迁移和侵袭的影响和机制。方法以人胃癌细胞系,胃癌、配对癌旁组织和正常人胃组织为研究对象;实时定量PCR和免疫组织化学法分析ZNF436在胃癌细胞系,...目的探讨锌指蛋白ZNF436在人胃癌细胞系和组织中的相对表达,及ZNF436对胃癌细胞MKN-28迁移和侵袭的影响和机制。方法以人胃癌细胞系,胃癌、配对癌旁组织和正常人胃组织为研究对象;实时定量PCR和免疫组织化学法分析ZNF436在胃癌细胞系,胃癌、配对癌旁组织和正常人胃组织中的相对表达;在线数据分析ZNF436的表达与预后关系;ZNF436-siRNA(实验组)、NC-siRNA(对照组)质粒转染人胃癌MKN028细胞,转染后48 h实时定量PCR验证ZNF436的敲降效率,Transwell实验分析敲降ZNF436后对实验组细胞迁移和侵袭影响;裸鼠转移瘤实验验证ZNF436体内对细胞转移的影响;免疫蛋白印迹实验验证ZNF436对WNT/β-catenin信号通路的影响。结果与正常胃上皮细胞系相比,ZNF436在人胃癌细胞系中高表达( P <0.001);同时与癌旁组织和正常人胃组织相比,ZNF436在胃癌组织中高表达( P <0.001);在线数据发现ZNF436的表达与胃癌的预后有关( P =0.0028);ZNF436基因表达越高,患者预后越差( P <0.001);与对照组相比,实验组细胞的迁移和侵袭能力明显受到抑制( P <0.001);实验组中裸鼠转移瘤的瘤大小和体积明显低于实验组( P < 0.001);低表达ZNF436后,WNT/β-catenin信号通路及其下游细胞因子明显受到抑制。结论ZNF436作为潜在癌基因在人胃癌组织中表达上调,通过促进WNT/β-catenin信号通路参与胃癌的进程。展开更多
A comparison was made for the deformation, microstructure, mechanical properties and formability of SUS436L stainless steel in tandem and reversible cold rolling processes. At first, the thermophysical parameters and ...A comparison was made for the deformation, microstructure, mechanical properties and formability of SUS436L stainless steel in tandem and reversible cold rolling processes. At first, the thermophysical parameters and stress-strain curves of SUS436L steel were measured in temperature range of 293-573 K and a flow stress model was regressed from the data of these curves. An analytical model based on the elasto-plastic finite element method was then established to simulate the tandem and the reversible cold rolling processes of SUS436L stainless steel strip where the flow stress model was introduced. The difference in shear strain distribution, microstructure, mechanical properties and formability of SUS436L steel strip in the two rolling processes was analyzed. The results showed that the larger shear strain, the enhanced intensity of γ fiber texture and the excellent formability of the strip can be easily obtained in the tandem rolling process with the larger work roll rather than the reversible rolling process with the smaller work roll.展开更多
基金supported by the National Natural Science Foundation of China (Grants No.51501041 and No.51671059)
文摘Passivity breakdown on 436 ferritic stainless steel(FSS) has been investigated in solutions containing different concentrations of chloride at 25?C and interpreted in terms of the point defect model(PDM). The measured near-normal distributions of passivity breakdown potentials for 436 FSS under experimental conditions are in good agreement with the calculated results according to the PDM. The linear dependence of breakdown potential on the square root of potential scanning rate, which was described by the PDM,provides the estimation of the critical concentration of condensed vacancies at the metal/film interface,which leads to the passivity breakdown. This value is in good agreement with that calculated from the microstructure properties of the alloy substrate and the barrier layer of the passive film. This study demonstrates the validity of the PDM in describing the passivity breakdown on 436 FSS in NaCl solutions.
文摘目的探讨锌指蛋白ZNF436在人胃癌细胞系和组织中的相对表达,及ZNF436对胃癌细胞MKN-28迁移和侵袭的影响和机制。方法以人胃癌细胞系,胃癌、配对癌旁组织和正常人胃组织为研究对象;实时定量PCR和免疫组织化学法分析ZNF436在胃癌细胞系,胃癌、配对癌旁组织和正常人胃组织中的相对表达;在线数据分析ZNF436的表达与预后关系;ZNF436-siRNA(实验组)、NC-siRNA(对照组)质粒转染人胃癌MKN028细胞,转染后48 h实时定量PCR验证ZNF436的敲降效率,Transwell实验分析敲降ZNF436后对实验组细胞迁移和侵袭影响;裸鼠转移瘤实验验证ZNF436体内对细胞转移的影响;免疫蛋白印迹实验验证ZNF436对WNT/β-catenin信号通路的影响。结果与正常胃上皮细胞系相比,ZNF436在人胃癌细胞系中高表达( P <0.001);同时与癌旁组织和正常人胃组织相比,ZNF436在胃癌组织中高表达( P <0.001);在线数据发现ZNF436的表达与胃癌的预后有关( P =0.0028);ZNF436基因表达越高,患者预后越差( P <0.001);与对照组相比,实验组细胞的迁移和侵袭能力明显受到抑制( P <0.001);实验组中裸鼠转移瘤的瘤大小和体积明显低于实验组( P < 0.001);低表达ZNF436后,WNT/β-catenin信号通路及其下游细胞因子明显受到抑制。结论ZNF436作为潜在癌基因在人胃癌组织中表达上调,通过促进WNT/β-catenin信号通路参与胃癌的进程。
基金National Natural Science Foundation of China and Baowu Steel Group Co., Ltd.(Grant No. U1660205).
文摘A comparison was made for the deformation, microstructure, mechanical properties and formability of SUS436L stainless steel in tandem and reversible cold rolling processes. At first, the thermophysical parameters and stress-strain curves of SUS436L steel were measured in temperature range of 293-573 K and a flow stress model was regressed from the data of these curves. An analytical model based on the elasto-plastic finite element method was then established to simulate the tandem and the reversible cold rolling processes of SUS436L stainless steel strip where the flow stress model was introduced. The difference in shear strain distribution, microstructure, mechanical properties and formability of SUS436L steel strip in the two rolling processes was analyzed. The results showed that the larger shear strain, the enhanced intensity of γ fiber texture and the excellent formability of the strip can be easily obtained in the tandem rolling process with the larger work roll rather than the reversible rolling process with the smaller work roll.
