【目的】探究丝裂原活化蛋白激酶激酶6(mitogen-activated protein kinase kinase6,MAP2K6)基因在湖羊不同发育阶段背最长肌组织中的表达水平,分析该基因的多态性与湖羊生长性状之间的相关性,以期为湖羊的生长性状分子育种提供新的标记...【目的】探究丝裂原活化蛋白激酶激酶6(mitogen-activated protein kinase kinase6,MAP2K6)基因在湖羊不同发育阶段背最长肌组织中的表达水平,分析该基因的多态性与湖羊生长性状之间的相关性,以期为湖羊的生长性状分子育种提供新的标记资源。【方法】利用实时荧光定量PCR检测MAP2K6基因在湖羊(n=15)不同发育阶段背最长肌组织中的表达情况;通过Illumina OvineSNP 50K BeadChip检测湖羊(n=3024)MAP2K6基因的单核苷酸多态性(SNP),利用一般线性模型分析MAP2K6基因SNP位点与湖羊(n=1974)生长性状间的关联性,并使用R语言corrplot包计算湖羊体重与各体尺指标的相关系数。【结果】实时荧光定量PCR检测结果显示,湖羊背最长肌组织中MAP2K6基因表达量在初生到4月龄阶段逐渐升高,且3、4月龄的表达量均极显著高于初生、45日龄和6月龄(P<0.01)。湖羊MAP2K6基因中共检测到2个位点:rs414959578G>A和rs426057803A>G。关联分析结果显示,MAP2K6基因rs414959578G>A位点对湖羊5月龄体重、体高、体斜长、胸围、胸深、胸宽、十字部高、腰角宽,以及6月龄胸围、背膘厚有显著或极显著影响(P<0.05;P<0.01);rs426057803A>G位点对湖羊3月龄管围,5月龄胸围、管围和十字部高以及6月龄背膘厚有显著或极显著影响(P<0.05;P<0.01)。相关性分析结果显示,湖羊体重与体尺指标间存在显著正相关(P<0.05),但6月龄湖羊体斜长与6月龄胸宽、腰角宽,5月龄管围与6月龄腰角宽均不存在显著相关(P>0.05)。【结论】MAP2K6基因与湖羊背最长肌的发育相关,rs414959578G>A和rs426057803A>G位点对湖羊生长性状有显著影响。研究结果可为湖羊生长性状分子标记的挖掘和利用提供一定的理论依据。展开更多
High-throughput genotyping tools can effectively promote molecular breeding in crops.In this study,genotyping by target sequencing(GBTS)system was utilized to develop a genome-wide liquid SNP chip for facilitating gen...High-throughput genotyping tools can effectively promote molecular breeding in crops.In this study,genotyping by target sequencing(GBTS)system was utilized to develop a genome-wide liquid SNP chip for facilitating genetics and breeding in melon(Cucumis melo L.),a globally cultivated economically important horticultural crop.Based on over eight million SNPs derived from 823 representative melon accessions,16K,8K,4K,2K,1K,500,250 and 125 informative SNPs were screened and evaluated for their polymorphisms,conservation of flanking sequences,and distributions.The set of 2K SNPs was found to be optimal for representing the maximum diversity with the lowest number of SNPs,and it was selected to develop the liquid chip,named“Melon2K”.Using Melon2K,more than 1500 SNPs were detected across 17 samples of five melon cultivars,and the phylogenetic relationships were clearly constructed.Within the same cultivar,genetic differences were also assessed between different samples.We evaluated the performance of Melon2K in genetic background selection during the breeding process,obtaining the introgression lines of interested trait with more than 97%genetic background of elite variety by only two rounds of backcrossing.These results suggest that Melon2K provides a cost-effective,efficient and reliable platform for genetic analysis and molecular breeding in melon.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.32102383,32225044 and 32130093)the Natural Science Foundation of Shandong Province(Grant No.ZR2021QC075)+1 种基金the Taishan Scholar Foundation of the People's Government of Shandong Province(Grant No.ts20190947)the Qingdao Agricultural University Doctoral Start-Up Fund。
文摘High-throughput genotyping tools can effectively promote molecular breeding in crops.In this study,genotyping by target sequencing(GBTS)system was utilized to develop a genome-wide liquid SNP chip for facilitating genetics and breeding in melon(Cucumis melo L.),a globally cultivated economically important horticultural crop.Based on over eight million SNPs derived from 823 representative melon accessions,16K,8K,4K,2K,1K,500,250 and 125 informative SNPs were screened and evaluated for their polymorphisms,conservation of flanking sequences,and distributions.The set of 2K SNPs was found to be optimal for representing the maximum diversity with the lowest number of SNPs,and it was selected to develop the liquid chip,named“Melon2K”.Using Melon2K,more than 1500 SNPs were detected across 17 samples of five melon cultivars,and the phylogenetic relationships were clearly constructed.Within the same cultivar,genetic differences were also assessed between different samples.We evaluated the performance of Melon2K in genetic background selection during the breeding process,obtaining the introgression lines of interested trait with more than 97%genetic background of elite variety by only two rounds of backcrossing.These results suggest that Melon2K provides a cost-effective,efficient and reliable platform for genetic analysis and molecular breeding in melon.
