Objective:To identify genotypes of Oesophagostmum venulosum(O.venulosum) prevailing in West Bengal,India by comparing variation of nucleotide sequences among 28S rRNA.Methods: PCR amplification of partial segment of 2...Objective:To identify genotypes of Oesophagostmum venulosum(O.venulosum) prevailing in West Bengal,India by comparing variation of nucleotide sequences among 28S rRNA.Methods: PCR amplification of partial segment of 28 S rRNA sequence and analysis of sequence amplified product by single strand conformation polymorphism(SSCP).Results:Two distinct conformers among male and female parasites were identified by PCR-SSCP analysis.Sequence analysis among conformers revealed the presence of five single nucleotide polymorphisms(SNP) in codon 64,66,86,125 and 146.Secondary RNA prediction structure showed that out of 5 SNPs,4 occurred at interior loop of RNA which confirmed evolutionary changes among isolates prevailing in this region.Conclusions:SNPs occured in different isolates of 0.venulosum might influence critical changes in rRNA folding pattern which influence evolutionary changes among isolates.展开更多
The monophyly of Diplura and its phylogenetic relationship with other hexapods are important for understanding the phylogeny of Hexapoda. The complete 18SrRNA gene and partial 28SrRNA gene (D3-D5 region) from 2 diplur...The monophyly of Diplura and its phylogenetic relationship with other hexapods are important for understanding the phylogeny of Hexapoda. The complete 18SrRNA gene and partial 28SrRNA gene (D3-D5 region) from 2 dipluran species (Campodeidae and Japygidae), 2 proturan species, 3 collembolan species, and 1 locust species were sequenced. Combining related se-quences in GenBank, phylogenetic trees of Hexapoda were constructed by MP method using a crustacean Artemia salina as an outgroup. The results indicated that: (i) the integrated data of 18SrDNA and 28SrDNA could provide better phylogenetic information, which well supported the monophyly of Diplura; (ii) Diplura had a close phylogenetic relationship to Protura with high boot-strap support.展开更多
文摘Objective:To identify genotypes of Oesophagostmum venulosum(O.venulosum) prevailing in West Bengal,India by comparing variation of nucleotide sequences among 28S rRNA.Methods: PCR amplification of partial segment of 28 S rRNA sequence and analysis of sequence amplified product by single strand conformation polymorphism(SSCP).Results:Two distinct conformers among male and female parasites were identified by PCR-SSCP analysis.Sequence analysis among conformers revealed the presence of five single nucleotide polymorphisms(SNP) in codon 64,66,86,125 and 146.Secondary RNA prediction structure showed that out of 5 SNPs,4 occurred at interior loop of RNA which confirmed evolutionary changes among isolates prevailing in this region.Conclusions:SNPs occured in different isolates of 0.venulosum might influence critical changes in rRNA folding pattern which influence evolutionary changes among isolates.
基金the National Natural Science Foundation of China (Grant No. 30130040) and Life Science Special Funds of Chinese Academy of Sciences.
文摘The monophyly of Diplura and its phylogenetic relationship with other hexapods are important for understanding the phylogeny of Hexapoda. The complete 18SrRNA gene and partial 28SrRNA gene (D3-D5 region) from 2 dipluran species (Campodeidae and Japygidae), 2 proturan species, 3 collembolan species, and 1 locust species were sequenced. Combining related se-quences in GenBank, phylogenetic trees of Hexapoda were constructed by MP method using a crustacean Artemia salina as an outgroup. The results indicated that: (i) the integrated data of 18SrDNA and 28SrDNA could provide better phylogenetic information, which well supported the monophyly of Diplura; (ii) Diplura had a close phylogenetic relationship to Protura with high boot-strap support.
