study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp.MDW-211.Preparative high performance liquid chromatography(HPLC)and other techniques were used to isol...study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp.MDW-211.Preparative high performance liquid chromatography(HPLC)and other techniques were used to isolate and purify the compounds,the structures of the compounds 1 were determined by high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),nuclear magnetic resonance(NMR)spectroscopy,X-ray and other methods,and the pro-angiogenic activity of the compounds was evaluated by zebrafish model.One new dihydroisocoumarin 1(4'S-hydroxyasperentin)and three known dihydroisocoumarins 2~4 were obtained and identified.Bioactivity assays revealed that compound 3 exhibited pro-angiogenic activity at concentrations of 20 and 40μmol/L,which is reported here for the first time.展开更多
基金Project supported by the National Natural Science Foundation of China(No.82204276)the Guangxi Natural Science Foundation(No.2025GXNSF-AA069557)+1 种基金the Guangxi Scholarship Fund of Guangxi Education Departmentthe Innovation Project of Guangxi Minzu University Graduate Education(No.gxmzu-chxs2024225)。
文摘study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp.MDW-211.Preparative high performance liquid chromatography(HPLC)and other techniques were used to isolate and purify the compounds,the structures of the compounds 1 were determined by high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),nuclear magnetic resonance(NMR)spectroscopy,X-ray and other methods,and the pro-angiogenic activity of the compounds was evaluated by zebrafish model.One new dihydroisocoumarin 1(4'S-hydroxyasperentin)and three known dihydroisocoumarins 2~4 were obtained and identified.Bioactivity assays revealed that compound 3 exhibited pro-angiogenic activity at concentrations of 20 and 40μmol/L,which is reported here for the first time.
