Previous studies have revealed the miR164 family and the miR164-targeted NAC transcription factor genes in rice(Oryza sativa)and Arabidopsis that play versatile roles in developmental processes and stress responses.In...Previous studies have revealed the miR164 family and the miR164-targeted NAC transcription factor genes in rice(Oryza sativa)and Arabidopsis that play versatile roles in developmental processes and stress responses.In wheat(Triticum aestivum L.),we found nine genetic loci of tae-miR164(tae-MIR164 a to i)producing two mature sequences that downregulate the expression of three newly identified target genes of TaNACs(TaNAC1,TaNAC11,and TaNAC14)by the cleavage of the respective mRNAs.Overexpression of tae-miR164 or one of its target genes(TaNAC14)demonstrated that the miR164-TaNAC14 module greatly affects root growth and development and stress(drought and salinity)tolerance in wheat seedlings,and TaNAC14 promotes root growth and development in wheat seedlings and enhances drought tolerance,while tae-miR164 inhibits root development and reduces drought and salinity tolerance by downregulating the expression of TaNAC14.These findings identify the miR164-TaNAC14 module as well as other taemiR164-regulated genes which can serve as new genetic resources for stress-resistance wheat breeding.展开更多
基金financially supported by the National Natural Science Foundation of China(32072003and 32072059)the Key Research and Development Program of Shaanxi Province,China(2021NY-079)。
文摘Previous studies have revealed the miR164 family and the miR164-targeted NAC transcription factor genes in rice(Oryza sativa)and Arabidopsis that play versatile roles in developmental processes and stress responses.In wheat(Triticum aestivum L.),we found nine genetic loci of tae-miR164(tae-MIR164 a to i)producing two mature sequences that downregulate the expression of three newly identified target genes of TaNACs(TaNAC1,TaNAC11,and TaNAC14)by the cleavage of the respective mRNAs.Overexpression of tae-miR164 or one of its target genes(TaNAC14)demonstrated that the miR164-TaNAC14 module greatly affects root growth and development and stress(drought and salinity)tolerance in wheat seedlings,and TaNAC14 promotes root growth and development in wheat seedlings and enhances drought tolerance,while tae-miR164 inhibits root development and reduces drought and salinity tolerance by downregulating the expression of TaNAC14.These findings identify the miR164-TaNAC14 module as well as other taemiR164-regulated genes which can serve as new genetic resources for stress-resistance wheat breeding.
