Leaf morphology constitutes a key component of the ideotype,and optimal leaf rolling contributes to compact plant architecture.Rapeseed(Brassica napus)is an important oilseed crop;however,the genetic mechanisms underl...Leaf morphology constitutes a key component of the ideotype,and optimal leaf rolling contributes to compact plant architecture.Rapeseed(Brassica napus)is an important oilseed crop;however,the genetic mechanisms underlying leaf shape development remain poorly understood,and corresponding germplasm resources for genetic improvement are limited.In this study,we identified a dominant mutant,INSIDE-ROLLING LEAF1(IRL1),which exhibits inward leaf rolling due to defective mesophyll cell development.The mutant also displays drooping siliques and a semi-dwarf phenotype,accompanied by a reduction of one to two effective branches.Through map-based cloning and functional complementation assays,we confirmed BnaC02G0201100ZS as the causal gene IRL1.This gene encodes LATERAL ORGAN BOUNDARIES DOMAIN6(BnaC02.LBD6).The phenotypic alterations in the IRL1 mutant result from elevated expression of BnaC02.LBD6,driven by a single nucleotide substitution within a DNA binding site in its promoter region.Overexpression of Bna C02.LBD6 recapitulated the IRL1 mutant phenotype,confirming its functional role.Haplotype analysis revealed a rare allelic variant in the BnaC02.LBD6 promoter associated with the unique leaf morphology of IRL1.Transcriptomic profiling indicated significant differential expression of genes involved in adaxial–abaxial leaf polarity establishment,secondary metabolic pathways,and hormone signaling networks.Our findings provide novel insights into the genetic regulation of leaf morphogenesis in rapeseed and offer valuable genetic resources for optimizing plant architecture in breeding programs.展开更多
目的 评估BC02佐剂配伍gE蛋白在老年小鼠模型中的免疫效性能,为优化老年群体带状疱疹疫苗设计提供实验依据。方法 将青年(6~8周龄)和老年(13月龄)BALB/c小鼠随机分为PBS组、gE+AS01B组和gE+BC02组(n=6/组),间隔4周,经大腿肌肉注射免疫2...目的 评估BC02佐剂配伍gE蛋白在老年小鼠模型中的免疫效性能,为优化老年群体带状疱疹疫苗设计提供实验依据。方法 将青年(6~8周龄)和老年(13月龄)BALB/c小鼠随机分为PBS组、gE+AS01B组和gE+BC02组(n=6/组),间隔4周,经大腿肌肉注射免疫2次(间隔4周)疫苗。采用ELISA检测血清gE特异性IgG及其亚类抗体水平,ELISpot分析脾淋巴细胞IFN-γ、IL-2和IL-4分泌能力,流式细胞术检测CD4^(+)T细胞亚群(IFN-γ^(+)、IL-2^(+)、IL-4^(+)、IL-17+)及记忆T细胞(IFN-γ^(+)、IL-2^(+))比例。结果 gE+BC02组在青年和老年小鼠中均诱导了显著升高的gE特异性IgG、IgG1和IgG2a抗体,且抗体水平无年龄差异。细胞免疫应答方面,老年小鼠中,gE+AS01B组诱导的IFN-γ^(+)CD4^(+)(0.188±0.08 vs 0.086±0.06)和IL-2^(+)CD4^(+)(0.448±0.07 vs 0.338±0.07)T细胞比例为gE+BC02疫苗的2.19和1.33倍;而青年小鼠gE+BC02组IL-17+CD4^(+)(0.144±0.03 vs 0.092±0.023)T细胞比例则为gE+AS01B组的1.57倍。记忆T细胞应答分析显示,gE+BC02组老年小鼠IFN-γ^(+)和IL-2^(+)记忆T细胞比例较青年组显著降低53.94%(0.456±0.14 vs 0.99±0.37)和41.83%(0.356±0.12 vs 0.612±0.24),其余无显著性差异。结论 BC02佐剂可有效激活老年小鼠的体液免疫应答,但其诱导的Th1型细胞免疫应答稍弱于AS01B佐剂,且均存在年龄相关差异。本研究为开发适合老年人群的带状疱疹疫苗提供了重要实验依据,提示未来可通过优化BC02佐剂配方来增强Th1型免疫应答。展开更多
基金funded by the National Key Research and Development Program of China(2021YFF1000100)the Fundamental Research Funds for the Central Universities,China(2662023PY004)。
文摘Leaf morphology constitutes a key component of the ideotype,and optimal leaf rolling contributes to compact plant architecture.Rapeseed(Brassica napus)is an important oilseed crop;however,the genetic mechanisms underlying leaf shape development remain poorly understood,and corresponding germplasm resources for genetic improvement are limited.In this study,we identified a dominant mutant,INSIDE-ROLLING LEAF1(IRL1),which exhibits inward leaf rolling due to defective mesophyll cell development.The mutant also displays drooping siliques and a semi-dwarf phenotype,accompanied by a reduction of one to two effective branches.Through map-based cloning and functional complementation assays,we confirmed BnaC02G0201100ZS as the causal gene IRL1.This gene encodes LATERAL ORGAN BOUNDARIES DOMAIN6(BnaC02.LBD6).The phenotypic alterations in the IRL1 mutant result from elevated expression of BnaC02.LBD6,driven by a single nucleotide substitution within a DNA binding site in its promoter region.Overexpression of Bna C02.LBD6 recapitulated the IRL1 mutant phenotype,confirming its functional role.Haplotype analysis revealed a rare allelic variant in the BnaC02.LBD6 promoter associated with the unique leaf morphology of IRL1.Transcriptomic profiling indicated significant differential expression of genes involved in adaxial–abaxial leaf polarity establishment,secondary metabolic pathways,and hormone signaling networks.Our findings provide novel insights into the genetic regulation of leaf morphogenesis in rapeseed and offer valuable genetic resources for optimizing plant architecture in breeding programs.
文摘目的 评估BC02佐剂配伍gE蛋白在老年小鼠模型中的免疫效性能,为优化老年群体带状疱疹疫苗设计提供实验依据。方法 将青年(6~8周龄)和老年(13月龄)BALB/c小鼠随机分为PBS组、gE+AS01B组和gE+BC02组(n=6/组),间隔4周,经大腿肌肉注射免疫2次(间隔4周)疫苗。采用ELISA检测血清gE特异性IgG及其亚类抗体水平,ELISpot分析脾淋巴细胞IFN-γ、IL-2和IL-4分泌能力,流式细胞术检测CD4^(+)T细胞亚群(IFN-γ^(+)、IL-2^(+)、IL-4^(+)、IL-17+)及记忆T细胞(IFN-γ^(+)、IL-2^(+))比例。结果 gE+BC02组在青年和老年小鼠中均诱导了显著升高的gE特异性IgG、IgG1和IgG2a抗体,且抗体水平无年龄差异。细胞免疫应答方面,老年小鼠中,gE+AS01B组诱导的IFN-γ^(+)CD4^(+)(0.188±0.08 vs 0.086±0.06)和IL-2^(+)CD4^(+)(0.448±0.07 vs 0.338±0.07)T细胞比例为gE+BC02疫苗的2.19和1.33倍;而青年小鼠gE+BC02组IL-17+CD4^(+)(0.144±0.03 vs 0.092±0.023)T细胞比例则为gE+AS01B组的1.57倍。记忆T细胞应答分析显示,gE+BC02组老年小鼠IFN-γ^(+)和IL-2^(+)记忆T细胞比例较青年组显著降低53.94%(0.456±0.14 vs 0.99±0.37)和41.83%(0.356±0.12 vs 0.612±0.24),其余无显著性差异。结论 BC02佐剂可有效激活老年小鼠的体液免疫应答,但其诱导的Th1型细胞免疫应答稍弱于AS01B佐剂,且均存在年龄相关差异。本研究为开发适合老年人群的带状疱疹疫苗提供了重要实验依据,提示未来可通过优化BC02佐剂配方来增强Th1型免疫应答。
