Taking the tender leaves of Oplopanax elatus as explants for the experiment,Uniform Design were used to test the most suitable media for callus induction,differentiation of shoot and rooting.The results showed that N6...Taking the tender leaves of Oplopanax elatus as explants for the experiment,Uniform Design were used to test the most suitable media for callus induction,differentiation of shoot and rooting.The results showed that N6 + 6-BA 0.5 mg·L-1 + 2,4-D 0.2 mg·L-1+Vc 10 mg·L-1 was fitted for callus induction,the medium for differentiation of shoot was N6 + 6-BA 4.5 mg·L-1 +NAA 0.1 mg·L-1+Vc 20 mg·L-1,the medium for rooting was 1/4MS + IBA 0.1mg·L-1 +NAA 0.1 mg·L-1.Tissue culture required different kinds of culture media in different phases.展开更多
文摘Taking the tender leaves of Oplopanax elatus as explants for the experiment,Uniform Design were used to test the most suitable media for callus induction,differentiation of shoot and rooting.The results showed that N6 + 6-BA 0.5 mg·L-1 + 2,4-D 0.2 mg·L-1+Vc 10 mg·L-1 was fitted for callus induction,the medium for differentiation of shoot was N6 + 6-BA 4.5 mg·L-1 +NAA 0.1 mg·L-1+Vc 20 mg·L-1,the medium for rooting was 1/4MS + IBA 0.1mg·L-1 +NAA 0.1 mg·L-1.Tissue culture required different kinds of culture media in different phases.
