Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of th...Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining展开更多
The zymogram purity of 5 inbred lines,7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis. Results indicate that...The zymogram purity of 5 inbred lines,7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis. Results indicate that the maize hybrid impurity is mainly composed of three parts:mixed with the seeds from parents or other source, contaminated with the seeds resulting from unknown pollination and variable in genetic background. It was found that the varietal purity is higher than the zymogram purity and they showed a linear relationship. The regression coefficient (r) between varietal purity and zymogram purity is 0.898 2. There is an average of 3.5% variable in the field. However,using a known rate of the mixed seeds, their regression coefficient is 0.999 9. It was also found that the examinational mix rate is 0.33% higher than the known mix rate. The seedling rate of the single cross hybrids is 85.71%,but that of hybrids mixed with their parental lines is only 77%. Therefore,it is reasonable to claim that zymogram purity is lower than the varietal purity.展开更多
文摘Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining
文摘The zymogram purity of 5 inbred lines,7 single cross hybrids and 7 single cross hybrids mixed with their parental lines was examined by a vertical discontinous polyacrylamide gel electrophoresis. Results indicate that the maize hybrid impurity is mainly composed of three parts:mixed with the seeds from parents or other source, contaminated with the seeds resulting from unknown pollination and variable in genetic background. It was found that the varietal purity is higher than the zymogram purity and they showed a linear relationship. The regression coefficient (r) between varietal purity and zymogram purity is 0.898 2. There is an average of 3.5% variable in the field. However,using a known rate of the mixed seeds, their regression coefficient is 0.999 9. It was also found that the examinational mix rate is 0.33% higher than the known mix rate. The seedling rate of the single cross hybrids is 85.71%,but that of hybrids mixed with their parental lines is only 77%. Therefore,it is reasonable to claim that zymogram purity is lower than the varietal purity.
