In order to assess college students'overall language proficiency,a new format,banked cloze,is included in the revised CET-4.Based on the previous studies on banked cloze in China,this paper discusses on how to con...In order to assess college students'overall language proficiency,a new format,banked cloze,is included in the revised CET-4.Based on the previous studies on banked cloze in China,this paper discusses on how to construct highly valid CET-4 banked cloze in line with the revised CET Syllabus(2006).展开更多
Objective:To address the prediction of hemorrhagic transformation(HT)after rt-PA thrombolysis in acute cerebral infarction(ACI)patients,a predictive model integrating data engineering,algorithm optimization,and modula...Objective:To address the prediction of hemorrhagic transformation(HT)after rt-PA thrombolysis in acute cerebral infarction(ACI)patients,a predictive model integrating data engineering,algorithm optimization,and modularity was constructed.This model resolves the technical limitation of traditional clinical statistical models that overemphasize analysis while neglecting deployment readiness,thereby providing a reusable technical solution for the implementation of medical risk prediction systems.Methods:A standardized dataset was built using data from 300 ACI patients who underwent rt-PA thrombolysis.Automated data cleaning,outlier correction,and feature engineering optimization(recursive feature elimination+variance inflation factor test)were applied to select core features.An L2-regularized logistic regression algorithm was employed to construct the prediction model,which was visualized via a nomogram.Multi-dimensional performance validation was conducted through cross-validation and probability calibration techniques.Results:The incidence of HT was20.0%.Multivariate analysis identified NIHSS score(OR=1.626),blood glucose(OR=1.662),platelet count(OR=0.975),age(OR=1.091),and history of diabetes(OR=0.343)as core predictors(P<0.05).Engineering validation demonstrated excellent discrimination(AUC=0.800,95%CI:0.752-0.856).After optimization with Platt scaling,the calibration curve was highly consistent with the actual probability,with a Brier score of0.092.The prediction time per sample was<50 ms,and the convergence efficiency of model training was improved by 30%.Conclusion:This model integrates core technologies including data preprocessing,feature optimization,and algorithm improvement,exhibiting low-latency,high stability,and deployability.It provides a standardized technical framework for transforming medical prediction models from statistical conclusions into engineered products.展开更多
Animal models are necessary to investigate the pathogenic features underlying motor neuron degeneration and for therapeutic development in amyotrophic lateral sclerosis（ALS）. Measures of model validity allow for a c...Animal models are necessary to investigate the pathogenic features underlying motor neuron degeneration and for therapeutic development in amyotrophic lateral sclerosis（ALS）. Measures of model validity allow for a critical interpretation of results from each model and caution from over-interpretation of experimental models. Face and construct validity refer to the similarity in phenotype and the proposed causal factor to the human disease, respectively. More recently developed models are restricted by limited phenotype characterization, yet new models hold promise for novel disease insights, thus highlighting their importance. In this article, we evaluate the features of face and construct validity of our new zebrafish model of environmentally-induced motor neuron degeneration and discuss this in the context of current environmental and genetic ALS models, including C9 orf72, mutant Cu/Zn superoxide dismutase 1 and TAR DNA-binding protein 43 mouse and zebrafish models. In this mini-review, we discuss the pros and cons to validity criteria in each model. Our zebrafish model of environmentally-induced motor neuron degeneration displays convincing features of face validity with many hallmarks of ALS-like features, and weakness in construct validity. However, the value of this model may lie in its potential to be more representative of the pathogenic features underlying sporadic ALS cases, where environmental factors may be more likely to be involved in disease etiology than single dominant gene mutations. It may be necessary to compare findings between different strains and species modeling specific genes or environmental factors to confirm findings from ALS animal models and tease out arbitrary strain-and overexpression-specific effects.展开更多
The main purpose of this study was to determine the validation of construct and reliability of the image instrument in educational institutions. A non-random sample selection of 314 high school students from Montemore...The main purpose of this study was to determine the validation of construct and reliability of the image instrument in educational institutions. A non-random sample selection of 314 high school students from Montemorelos, Nuevo Leon was made. An instrument with 25 statements was created to measure the image of The University of Montemorelos （reliability a = 0.956）. The methodologies that were utilized in the validation were factorial analysis and confirmatory analysis, with the latter made with structural equations. In the factorial analysis, it was observed that the statements were grouped into four dimensions, as it was initially planned. With the exception of one item, all the other statements were grouped in the proposed dimension. In the confirmatory validation utilizing structural equations, all the selected criteria were fulfilled.展开更多
Listening Comprehension test of CET-6 has a great adjustment in its item types and weighting of score since December,2015, however, there have been few researches concerning the validity of newly innovated CET-6 liste...Listening Comprehension test of CET-6 has a great adjustment in its item types and weighting of score since December,2015, however, there have been few researches concerning the validity of newly innovated CET-6 listening comprehension test.This paper tries to explore the construct validity and content validity of it. Based on the comparison between the test contents of lis-tening and the test requirements in CET syllabus,it is found that CET-6 listening shows good content relevance. Structural equa-tion modeling(SEM) was used to investigate the construct validity of CET-6 listening, which demonstrates that it mainly tests stu-dents' ability to understand details and inference, while ignoring socio-linguistic competence and psych-physiological compe-tence. Therefore, certain changes are desirable to increase the validity of the listening part of CET-6.展开更多
Background:Recently,Mullen et al.(2011)presented an 8-item version of the Physical Activity Enjoyment Scale(PACES)that provides a valid instrument for assessing enjoyment in physical activity.The present paper investi...Background:Recently,Mullen et al.(2011)presented an 8-item version of the Physical Activity Enjoyment Scale(PACES)that provides a valid instrument for assessing enjoyment in physical activity.The present paper investigated the psychometric properties of a Portuguese adaptation of PACES.Methods:After a process of back-to-back translation into Portuguese,395 members of fitness centers who ranged in age from 18 to 66 years(31.11§8.90,mean§SD)completed the translated version of the PACES.On average,participants had 3.2 years of experience in fitness group classes and practiced for approximately 3.3 times per week.Results:An initial exploratory factor analysis(n=139)revealed a unidimensional structure with factor loadings ranging from 0.79 to 0.89.Results also showed acceptable internal consistency.A confirmatory factor analysis in an independent sample(n=256)provided additional support for the unidimensional structure of the questionnaire.In addition,moderate positive correlations between enjoyment and intrinsic and identified regulation,and moderate negative correlations between enjoyment and external and amotivation,demonstrate the convergent validity of the instrument.Finally,measurement invariance between 2 independent samples was also found.Conclusion:The 8-item Portuguese version of PACES is a valid and reliable instrument for measuring enjoyment of physical activity in Portuguese adult fitness exercisers,and it is therefore suitable to use as a measure of affect in exercise adherence interventions studies.展开更多
Increasing occurrences of mental health problems such as depression,anxiety and stress amongst workers presents practical demands for employers and occupational health practitioners to assess the self-perceived depres...Increasing occurrences of mental health problems such as depression,anxiety and stress amongst workers presents practical demands for employers and occupational health practitioners to assess the self-perceived depression,anxiety and stress in workers with reliable and valid instruments.The present study aims to validate the Malay version of the short form Depression Anxiety Stress Scale (DASS-21) among Malaysian workers designed to screen the self-reported of depression,anxiety and stress among workers in Malaysia.Eighty-eight workers of two major automotive assembly plants in Pekan,Pahang consented to participate in the study and was administered the Malay version of the DASS-21.Translation (English-Malay) and back translation (Malay-English) of the DASS-21 was made to ensure the face validity of the questionnaire.Reliability was determined using Cronbach's alpha for internal consistency whilst construct validity was assessed using the exploratory factor analysis (principal component analysis with varimax rotation).The results indicated that the Cronbach's alpha coefficients were acceptable for DASS-Depression (0.81),DASS-Anxiety (0.85) and DASS-Stress (0.85).Exploratory factor analysis showed three meaningful common factors that could explain the three theoretical dimensions or constructs of the DASS-21.The validation study suggests that the Malay version of DASS-21 is reliable and valid screening instruments for assessing self-reported depression,anxiety and stress in Malaysian working population.展开更多
Writing test , wins its popularity in measuring the mastery of one's language ability. In view of the significant role writing playing in a test , some considerations on writing test construction are presented in ...Writing test , wins its popularity in measuring the mastery of one's language ability. In view of the significant role writing playing in a test , some considerations on writing test construction are presented in this paper which anticipates the effective ways for measuring one's complex language ability of application.展开更多
The Server-Based Certificate Validation Protocol allows PKI clients to delegate to a server the construction or validation of certification paths. The protocol’s specification focuses on the communication between the...The Server-Based Certificate Validation Protocol allows PKI clients to delegate to a server the construction or validation of certification paths. The protocol’s specification focuses on the communication between the server and the client and its security. It does not discuss how the servers can efficiently locate the necessary PKI resources like certificate or certificate revocation lists. In this paper we concentrate on this topic. We present a simple and effective method to facilitate locating and using various PKI resources by the servers, without modifying the protocol. We use the extension mechanism of the protocol for notifying the servers about PKI repositories, certificates, and revocations. We specify the tasks of the servers and certificate issu-ers and define the messages that are exchanged between them. A proof of concept is given by implementing an SCVP server, a client, and the proposed method in Java.展开更多
Objective:This study aimed to prepare Golgi protein-73(GP73)-DC vaccine by constructing human lentiviral vector of GP73and transfecting this vector in vitro into a murine dendritic cell line DC 2.4.This will lay the f...Objective:This study aimed to prepare Golgi protein-73(GP73)-DC vaccine by constructing human lentiviral vector of GP73and transfecting this vector in vitro into a murine dendritic cell line DC 2.4.This will lay the foundation for further study on targeted therapy of hepatocellular carcinoma using GP73-DC vaccine.Methods:Human GP73 gene was cloned into lentivirus GV358vector and validated by polymerase chain reaction(PCR)and sequencing.The validated GV358-GP73vector was then cotransfected with pHelper 1.0plasmid into 293Tcells,in order to prepare lentivirus GP73vector.Finally,the protein expression of GP73 was achieved by transfecting the lentivirus GP73vector into DC 2.4cells in vitro and determined by Western blotting.Results:PCR and sequencing confirmed that the GV358-GP73lentivirus vector was constructed correctly,and the titer level reached 2×108TU/mL.After transfection in DC 2.4cells,the GP73protein level was significantly enhanced.Conclusion:The lentivirus GV538-GP73vector was constructed successfully,and could be effectively expressed in DC 2.4cells.展开更多
This paper aims to explore the construct validity of long dialogue multiple-choice (MC) listening comprehension tests through examining the retrospective verbal reports of eight Chinese EFL test-takers at tertiary lev...This paper aims to explore the construct validity of long dialogue multiple-choice (MC) listening comprehension tests through examining the retrospective verbal reports of eight Chinese EFL test-takers at tertiary level. It is found that the grammatical knowledge, discourse knowledge, pragmatic knowledge and sociolinguistic knowledge of language competence and all the cognitive and metacognitive strategies proposed in the framework of listening constructs are measured in the tests, which supports the constr...展开更多
文摘In order to assess college students'overall language proficiency,a new format,banked cloze,is included in the revised CET-4.Based on the previous studies on banked cloze in China,this paper discusses on how to construct highly valid CET-4 banked cloze in line with the revised CET Syllabus(2006).
文摘Objective:To address the prediction of hemorrhagic transformation(HT)after rt-PA thrombolysis in acute cerebral infarction(ACI)patients,a predictive model integrating data engineering,algorithm optimization,and modularity was constructed.This model resolves the technical limitation of traditional clinical statistical models that overemphasize analysis while neglecting deployment readiness,thereby providing a reusable technical solution for the implementation of medical risk prediction systems.Methods:A standardized dataset was built using data from 300 ACI patients who underwent rt-PA thrombolysis.Automated data cleaning,outlier correction,and feature engineering optimization(recursive feature elimination+variance inflation factor test)were applied to select core features.An L2-regularized logistic regression algorithm was employed to construct the prediction model,which was visualized via a nomogram.Multi-dimensional performance validation was conducted through cross-validation and probability calibration techniques.Results:The incidence of HT was20.0%.Multivariate analysis identified NIHSS score(OR=1.626),blood glucose(OR=1.662),platelet count(OR=0.975),age(OR=1.091),and history of diabetes(OR=0.343)as core predictors(P<0.05).Engineering validation demonstrated excellent discrimination(AUC=0.800,95%CI:0.752-0.856).After optimization with Platt scaling,the calibration curve was highly consistent with the actual probability,with a Brier score of0.092.The prediction time per sample was<50 ms,and the convergence efficiency of model training was improved by 30%.Conclusion:This model integrates core technologies including data preprocessing,feature optimization,and algorithm improvement,exhibiting low-latency,high stability,and deployability.It provides a standardized technical framework for transforming medical prediction models from statistical conclusions into engineered products.
基金supported by a grant from Estate of Luther Allyn Shourds Dean,No.20R17162(to CAS)
文摘Animal models are necessary to investigate the pathogenic features underlying motor neuron degeneration and for therapeutic development in amyotrophic lateral sclerosis（ALS）. Measures of model validity allow for a critical interpretation of results from each model and caution from over-interpretation of experimental models. Face and construct validity refer to the similarity in phenotype and the proposed causal factor to the human disease, respectively. More recently developed models are restricted by limited phenotype characterization, yet new models hold promise for novel disease insights, thus highlighting their importance. In this article, we evaluate the features of face and construct validity of our new zebrafish model of environmentally-induced motor neuron degeneration and discuss this in the context of current environmental and genetic ALS models, including C9 orf72, mutant Cu/Zn superoxide dismutase 1 and TAR DNA-binding protein 43 mouse and zebrafish models. In this mini-review, we discuss the pros and cons to validity criteria in each model. Our zebrafish model of environmentally-induced motor neuron degeneration displays convincing features of face validity with many hallmarks of ALS-like features, and weakness in construct validity. However, the value of this model may lie in its potential to be more representative of the pathogenic features underlying sporadic ALS cases, where environmental factors may be more likely to be involved in disease etiology than single dominant gene mutations. It may be necessary to compare findings between different strains and species modeling specific genes or environmental factors to confirm findings from ALS animal models and tease out arbitrary strain-and overexpression-specific effects.
文摘The main purpose of this study was to determine the validation of construct and reliability of the image instrument in educational institutions. A non-random sample selection of 314 high school students from Montemorelos, Nuevo Leon was made. An instrument with 25 statements was created to measure the image of The University of Montemorelos （reliability a = 0.956）. The methodologies that were utilized in the validation were factorial analysis and confirmatory analysis, with the latter made with structural equations. In the factorial analysis, it was observed that the statements were grouped into four dimensions, as it was initially planned. With the exception of one item, all the other statements were grouped in the proposed dimension. In the confirmatory validation utilizing structural equations, all the selected criteria were fulfilled.
文摘Listening Comprehension test of CET-6 has a great adjustment in its item types and weighting of score since December,2015, however, there have been few researches concerning the validity of newly innovated CET-6 listening comprehension test.This paper tries to explore the construct validity and content validity of it. Based on the comparison between the test contents of lis-tening and the test requirements in CET syllabus,it is found that CET-6 listening shows good content relevance. Structural equa-tion modeling(SEM) was used to investigate the construct validity of CET-6 listening, which demonstrates that it mainly tests stu-dents' ability to understand details and inference, while ignoring socio-linguistic competence and psych-physiological compe-tence. Therefore, certain changes are desirable to increase the validity of the listening part of CET-6.
文摘Background:Recently,Mullen et al.(2011)presented an 8-item version of the Physical Activity Enjoyment Scale(PACES)that provides a valid instrument for assessing enjoyment in physical activity.The present paper investigated the psychometric properties of a Portuguese adaptation of PACES.Methods:After a process of back-to-back translation into Portuguese,395 members of fitness centers who ranged in age from 18 to 66 years(31.11§8.90,mean§SD)completed the translated version of the PACES.On average,participants had 3.2 years of experience in fitness group classes and practiced for approximately 3.3 times per week.Results:An initial exploratory factor analysis(n=139)revealed a unidimensional structure with factor loadings ranging from 0.79 to 0.89.Results also showed acceptable internal consistency.A confirmatory factor analysis in an independent sample(n=256)provided additional support for the unidimensional structure of the questionnaire.In addition,moderate positive correlations between enjoyment and intrinsic and identified regulation,and moderate negative correlations between enjoyment and external and amotivation,demonstrate the convergent validity of the instrument.Finally,measurement invariance between 2 independent samples was also found.Conclusion:The 8-item Portuguese version of PACES is a valid and reliable instrument for measuring enjoyment of physical activity in Portuguese adult fitness exercisers,and it is therefore suitable to use as a measure of affect in exercise adherence interventions studies.
基金support from the IRPA research grant of the Ministry of Science,Technology and Innovation(MOSTI) in the Eighth Malaysia Plan(8-MP) budget(project no:06-02-05-2079-PR0061/09-03)
文摘Increasing occurrences of mental health problems such as depression,anxiety and stress amongst workers presents practical demands for employers and occupational health practitioners to assess the self-perceived depression,anxiety and stress in workers with reliable and valid instruments.The present study aims to validate the Malay version of the short form Depression Anxiety Stress Scale (DASS-21) among Malaysian workers designed to screen the self-reported of depression,anxiety and stress among workers in Malaysia.Eighty-eight workers of two major automotive assembly plants in Pekan,Pahang consented to participate in the study and was administered the Malay version of the DASS-21.Translation (English-Malay) and back translation (Malay-English) of the DASS-21 was made to ensure the face validity of the questionnaire.Reliability was determined using Cronbach's alpha for internal consistency whilst construct validity was assessed using the exploratory factor analysis (principal component analysis with varimax rotation).The results indicated that the Cronbach's alpha coefficients were acceptable for DASS-Depression (0.81),DASS-Anxiety (0.85) and DASS-Stress (0.85).Exploratory factor analysis showed three meaningful common factors that could explain the three theoretical dimensions or constructs of the DASS-21.The validation study suggests that the Malay version of DASS-21 is reliable and valid screening instruments for assessing self-reported depression,anxiety and stress in Malaysian working population.
文摘Writing test , wins its popularity in measuring the mastery of one's language ability. In view of the significant role writing playing in a test , some considerations on writing test construction are presented in this paper which anticipates the effective ways for measuring one's complex language ability of application.
文摘The Server-Based Certificate Validation Protocol allows PKI clients to delegate to a server the construction or validation of certification paths. The protocol’s specification focuses on the communication between the server and the client and its security. It does not discuss how the servers can efficiently locate the necessary PKI resources like certificate or certificate revocation lists. In this paper we concentrate on this topic. We present a simple and effective method to facilitate locating and using various PKI resources by the servers, without modifying the protocol. We use the extension mechanism of the protocol for notifying the servers about PKI repositories, certificates, and revocations. We specify the tasks of the servers and certificate issu-ers and define the messages that are exchanged between them. A proof of concept is given by implementing an SCVP server, a client, and the proposed method in Java.
基金supported by the National Natural Science Foundation of China (No.81360347)the Key University and College Project of Guangxi Provisional Department of Education(No.ZD2014027).
文摘Objective:This study aimed to prepare Golgi protein-73(GP73)-DC vaccine by constructing human lentiviral vector of GP73and transfecting this vector in vitro into a murine dendritic cell line DC 2.4.This will lay the foundation for further study on targeted therapy of hepatocellular carcinoma using GP73-DC vaccine.Methods:Human GP73 gene was cloned into lentivirus GV358vector and validated by polymerase chain reaction(PCR)and sequencing.The validated GV358-GP73vector was then cotransfected with pHelper 1.0plasmid into 293Tcells,in order to prepare lentivirus GP73vector.Finally,the protein expression of GP73 was achieved by transfecting the lentivirus GP73vector into DC 2.4cells in vitro and determined by Western blotting.Results:PCR and sequencing confirmed that the GV358-GP73lentivirus vector was constructed correctly,and the titer level reached 2×108TU/mL.After transfection in DC 2.4cells,the GP73protein level was significantly enhanced.Conclusion:The lentivirus GV538-GP73vector was constructed successfully,and could be effectively expressed in DC 2.4cells.
基金a small part of the research project"A Chronological Study of the CET Washback"(07BYY030)supported by the National Philosophy and Social Science Foundation of China . The teamleader is Gu Xiangdong.
文摘This paper aims to explore the construct validity of long dialogue multiple-choice (MC) listening comprehension tests through examining the retrospective verbal reports of eight Chinese EFL test-takers at tertiary level. It is found that the grammatical knowledge, discourse knowledge, pragmatic knowledge and sociolinguistic knowledge of language competence and all the cognitive and metacognitive strategies proposed in the framework of listening constructs are measured in the tests, which supports the constr...
