A new method was proposed to study the dynamics of stem form of matural Korean pine (Pinus koraiensis) trec. In natural Korean Pine forest, the occurring time of maximum height and diameter is very difference. This pa...A new method was proposed to study the dynamics of stem form of matural Korean pine (Pinus koraiensis) trec. In natural Korean Pine forest, the occurring time of maximum height and diameter is very difference. This paper connected stem form to stage of tree growth to analysis the form dynamic of Korean pine. The monomolecular equation was chosen as the stem model. The result shows that the maximum growth year of natural Korean pine is earlier than diameter.展开更多
Taper equations are an important tool in estimating stem volumes at a multi-product level for sustainable forest management.Nine taper equations are tested from three categories to estimate diameter at specified point...Taper equations are an important tool in estimating stem volumes at a multi-product level for sustainable forest management.Nine taper equations are tested from three categories to estimate diameter at specified point on the stem,height at specified diameter,volumes of any desired portion of the stem,and whole tree volume of Taurus fir in Taurus Mountains of Turkey.To account for autocorrelation and multicollinearity present among multiple stem data observations collected from the same tree.proper statistical approaches were used in model fitting.Comparisons are made to determine which equation provides the best overall fit to all data based on four goodness-of-fit statistics,Coefficient of determination(R2);Root mean square error(RMSE),Akaike’s Information Criterion(AIC),and Bayesian information criterion(BIC).Results indicated that all taper equations tested could be used to accurately estimate section diameter at given height and stem volume.Clark et al.’s taper equation provided better results than the others for Taurus fir when an additional stem diameter observation at 5.30 m was available.Segmented and variable-form taper models consistently provided better results than the simple taper models except for Max and Burkhart’s model.Fang et al.’s and Kozak’s taper models showed equally good performance to describe stem taper and to predict tree stem volume.Therefore,these taper equations are able to be used to estimate diameter and volume for Taurus fir trees,if an upper stem diameter measurement was not available.展开更多
Acacia mangium Willd. and Acacia auriculiformis A. Cunn. ex. Benth. are two of the fast-growing tropical acacias which have received priority for genetic assessment and improvement. They were therefore proposed for ti...Acacia mangium Willd. and Acacia auriculiformis A. Cunn. ex. Benth. are two of the fast-growing tropical acacias which have received priority for genetic assessment and improvement. They were therefore proposed for timber production in a short rotation in Malaysia but were impeded by the multiple leaders (ML) formation which limited their value as sawn timber. This work attempts to investigate the causes of ML formation in four genotypes of A. mangium and A. auriculiformis as related to apical dominance, nutrition and plant growth hormones and their correlations. The effects of 6-benzylaminopurine and decapitation on ML formation of these genotypes were also investigated. 6-benzylaminopurine (BAP) as a foliar spray and also decapitation did not stimulate ML formation. However, they significantly increased the number of branches and reduced all other growth traits including height. However, basal diameter was not affected by decapitation. The effects of BAP increased consistently with increasing level of application. BAP at 1500 mg·Lǃ resulted in mortality of A. mangium provenances while A. auriculiformis provenances survived and grew normally. Some of the BAP treated plants of both species developed juvenile pinnate leaves. Decapitation of the apical bud resulted in the activation of the lateral bud immediately below the point of decapitation.展开更多
Aim: This study aimed to investigate the protective effects of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs) against Aβ<sub>1-42</sub>-induced oligodendrocytes (OL) damage. ...Aim: This study aimed to investigate the protective effects of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs) against Aβ<sub>1-42</sub>-induced oligodendrocytes (OL) damage. Methods: Immunofluorescence was used for the detection of myelin-associated glycoprotein (MAG), a characteristic protein of rat oligodendrocytes (OLN-93 cells). To evaluate the potential protective effects of SSFs on OLN-93 cells injured by Aβ<sub>1-42</sub>, an injury model was established by subjecting OLN-93 cells to Aβ<sub>1-42</sub> exposed. Cell morphology was examined using an inverted microscope, while cell viability was assessed using the colorimetric method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Additionally, lactate dehydrogenase (LDH) was measured using the pyruvic acid reduction assay. The Ginkgo biloba leaf extract (GBE) injection was used as a positive control. Results: A total of >95% of the MAG immunofluorescence-positive cells were identified as oligodendrocytes. Gradually increasing concentrations of SSFs impaired the cells, and the maximum nondetrimental dose for OLN-93 cells was 75 mg/L. This study assessed the effects of SSFs on OLN-93 cells damaged by Aβ<sub>1-42</sub>. The results indicated that SSFs significantly improved OLN-93 cell morphological abnormal changes, increased the OLN-93 cell survival rate, and reduced LDH release. Conclusion: SSFs can alleviate Aβ<sub>1-42</sub>-induced damage of OL.展开更多
Cell-based technologies are used as a therapeutic strategy in spinal cord injury(SCI). Mesenchymal stem cells(MSCs), which secrete various neurotrophic factors and cytokines, have immunomodulatory, anti-apoptotic and ...Cell-based technologies are used as a therapeutic strategy in spinal cord injury(SCI). Mesenchymal stem cells(MSCs), which secrete various neurotrophic factors and cytokines, have immunomodulatory, anti-apoptotic and anti-inflammatory effects, modulate reactivity/phenotype of astrocytes and the microglia, thereby promoting neuroregeneration seem to be the most promising. The therapeutic effect of MSCs is due to a paracrine mechanism of their action, therefore the survival of MSCs and their secretory phenotype is of particular importance. Nevertheless, these data are not always reported in efficacy studies of MSC therapy in SCI. Here, we provide a review with summaries of preclinical trials data evaluating the efficacy of MSCs in animal models of SCI. Based on the data collected, we have tried(1) to establish the behavior of MSCs after transplantation in SCI with an evaluation of cell survival, migration potential, distribution in the area of injured and intact tissue and possible differentiation;(2) to determine the effects MSCs on neuronal microenvironment and correlate them with the efficacy of functional recovery in SCI;(3) to ascertain the conditions under which MSCs demonstrate their best survival and greatest efficacy.展开更多
Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed col...Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed colony-forming unit-fibroblasts, and could be expanded by supple- mentation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from 13-galactosidase transgenic mice were also transplanted into focal ischemic brain （right corpus striatum） of C57BL/6J mice. At 8 weeks, cells positive for LacZ and 13-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both 13-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.展开更多
文摘A new method was proposed to study the dynamics of stem form of matural Korean pine (Pinus koraiensis) trec. In natural Korean Pine forest, the occurring time of maximum height and diameter is very difference. This paper connected stem form to stage of tree growth to analysis the form dynamic of Korean pine. The monomolecular equation was chosen as the stem model. The result shows that the maximum growth year of natural Korean pine is earlier than diameter.
基金the part of Ph D dissertation and funded by the Suleyman Demirel University-Teaching Staff Training Program (Project number: OYP05250-DR-14)。
文摘Taper equations are an important tool in estimating stem volumes at a multi-product level for sustainable forest management.Nine taper equations are tested from three categories to estimate diameter at specified point on the stem,height at specified diameter,volumes of any desired portion of the stem,and whole tree volume of Taurus fir in Taurus Mountains of Turkey.To account for autocorrelation and multicollinearity present among multiple stem data observations collected from the same tree.proper statistical approaches were used in model fitting.Comparisons are made to determine which equation provides the best overall fit to all data based on four goodness-of-fit statistics,Coefficient of determination(R2);Root mean square error(RMSE),Akaike’s Information Criterion(AIC),and Bayesian information criterion(BIC).Results indicated that all taper equations tested could be used to accurately estimate section diameter at given height and stem volume.Clark et al.’s taper equation provided better results than the others for Taurus fir when an additional stem diameter observation at 5.30 m was available.Segmented and variable-form taper models consistently provided better results than the simple taper models except for Max and Burkhart’s model.Fang et al.’s and Kozak’s taper models showed equally good performance to describe stem taper and to predict tree stem volume.Therefore,these taper equations are able to be used to estimate diameter and volume for Taurus fir trees,if an upper stem diameter measurement was not available.
文摘Acacia mangium Willd. and Acacia auriculiformis A. Cunn. ex. Benth. are two of the fast-growing tropical acacias which have received priority for genetic assessment and improvement. They were therefore proposed for timber production in a short rotation in Malaysia but were impeded by the multiple leaders (ML) formation which limited their value as sawn timber. This work attempts to investigate the causes of ML formation in four genotypes of A. mangium and A. auriculiformis as related to apical dominance, nutrition and plant growth hormones and their correlations. The effects of 6-benzylaminopurine and decapitation on ML formation of these genotypes were also investigated. 6-benzylaminopurine (BAP) as a foliar spray and also decapitation did not stimulate ML formation. However, they significantly increased the number of branches and reduced all other growth traits including height. However, basal diameter was not affected by decapitation. The effects of BAP increased consistently with increasing level of application. BAP at 1500 mg·Lǃ resulted in mortality of A. mangium provenances while A. auriculiformis provenances survived and grew normally. Some of the BAP treated plants of both species developed juvenile pinnate leaves. Decapitation of the apical bud resulted in the activation of the lateral bud immediately below the point of decapitation.
文摘Aim: This study aimed to investigate the protective effects of flavonoids from the stem and leaves of Scutellaria baicalensis Georgi (SSFs) against Aβ<sub>1-42</sub>-induced oligodendrocytes (OL) damage. Methods: Immunofluorescence was used for the detection of myelin-associated glycoprotein (MAG), a characteristic protein of rat oligodendrocytes (OLN-93 cells). To evaluate the potential protective effects of SSFs on OLN-93 cells injured by Aβ<sub>1-42</sub>, an injury model was established by subjecting OLN-93 cells to Aβ<sub>1-42</sub> exposed. Cell morphology was examined using an inverted microscope, while cell viability was assessed using the colorimetric method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Additionally, lactate dehydrogenase (LDH) was measured using the pyruvic acid reduction assay. The Ginkgo biloba leaf extract (GBE) injection was used as a positive control. Results: A total of >95% of the MAG immunofluorescence-positive cells were identified as oligodendrocytes. Gradually increasing concentrations of SSFs impaired the cells, and the maximum nondetrimental dose for OLN-93 cells was 75 mg/L. This study assessed the effects of SSFs on OLN-93 cells damaged by Aβ<sub>1-42</sub>. The results indicated that SSFs significantly improved OLN-93 cell morphological abnormal changes, increased the OLN-93 cell survival rate, and reduced LDH release. Conclusion: SSFs can alleviate Aβ<sub>1-42</sub>-induced damage of OL.
基金supported by a grant from the Russian Foundation for Basic Research,No.16-34-60101(to YOM)a grant from the Ministry of Education and Science of the Russian Federation,No.20.5175.2017/6.7(to AAR)performed in accordance with the Program of Competitive Growth of the Kazan Federal University
文摘Cell-based technologies are used as a therapeutic strategy in spinal cord injury(SCI). Mesenchymal stem cells(MSCs), which secrete various neurotrophic factors and cytokines, have immunomodulatory, anti-apoptotic and anti-inflammatory effects, modulate reactivity/phenotype of astrocytes and the microglia, thereby promoting neuroregeneration seem to be the most promising. The therapeutic effect of MSCs is due to a paracrine mechanism of their action, therefore the survival of MSCs and their secretory phenotype is of particular importance. Nevertheless, these data are not always reported in efficacy studies of MSC therapy in SCI. Here, we provide a review with summaries of preclinical trials data evaluating the efficacy of MSCs in animal models of SCI. Based on the data collected, we have tried(1) to establish the behavior of MSCs after transplantation in SCI with an evaluation of cell survival, migration potential, distribution in the area of injured and intact tissue and possible differentiation;(2) to determine the effects MSCs on neuronal microenvironment and correlate them with the efficacy of functional recovery in SCI;(3) to ascertain the conditions under which MSCs demonstrate their best survival and greatest efficacy.
基金supported by the National Natural Science Foundation of China,No.30471836
文摘Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed colony-forming unit-fibroblasts, and could be expanded by supple- mentation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from 13-galactosidase transgenic mice were also transplanted into focal ischemic brain （right corpus striatum） of C57BL/6J mice. At 8 weeks, cells positive for LacZ and 13-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both 13-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.
