Adhesive patches offer an effective approach for wound closure,making them highly suitable for biomedical applications.However,conventional patches often face limitations such as dual-sided adhesion,lack of shape adap...Adhesive patches offer an effective approach for wound closure,making them highly suitable for biomedical applications.However,conventional patches often face limitations such as dual-sided adhesion,lack of shape adaptability,and limited maneuverability,which restrict their applications in deeper tissues.In this paper,we develop a magnetic patch robot(PatchBot),for targeted Janus adhesion with tissues.The PatchBot features a unique triple-layer structure,with adhesive,shape-morphing,and anti-adhesive layers,each fulfilling roles to support targeted attachment,enable shape transformation,and prevent unwanted adhesion to surrounding tissues.The Janus adhesion of the PatchBot was extensively demonstrated across a variety of tissues.A localized near-infrared(NIR)laser irradiation was used to induce programmable shape transformations.Magnetic actuation of the PatchBot for targeted adhesion was successfully demonstrated in ex vivo porcine stomach tissue.NIR light-activated shape-morphing and multimodal magnetic actuation significantly enhance its maneuverability and adaptability in confined in vivo environments while ensuring the structural integrity of the adhesive surface during deployment.This proof-of-concept study demonstrates the feasibility of using PatchBot for targeted wound adhesion,showing its potential for minimally invasive,precision therapies in complex in vivo environments.展开更多
Objective:To find a viable alternative to reduce the number of doses required for the patients with post-traumatic stress disorder(PTSD),and to improve efficacy and patient compliance.Methods: In this study,we used gi...Objective:To find a viable alternative to reduce the number of doses required for the patients with post-traumatic stress disorder(PTSD),and to improve efficacy and patient compliance.Methods: In this study,we used ginger oil,a phytochemical with potential therapeutic properties,to prepare ginger oil patches.High-performance liquid chromatography(HPLC)was used to quantify the main active component of ginger oil,6-gingerol.Transdermal absorption experiments were conducted to optimize the various pressure-sensitive adhesives and permeation enhancers,including their type and concentration.Subsequently,the ginger oil patches were optimized and subjected to content determination and property evaluations.A PTSD mouse model was established using the foot-shock method.The therapeutic effect of ginger oil patches on PTSD was assessed through pathological sections,behavioral tests,and the evaluation of biomarkers such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),brain-derived neurotrophic factor(BDNF),and melatonin(MT).Results: The results demonstrated that ginger oil patches exerted therapeutic effects against PTSD by inhibiting inflammatory responses and modulating MT and BDNF levels.Pharmacokinetic experiments revealed that ginger oil patches maintained a stable blood drug concentration for at least one day,addressing the rapid metabolism drawback of 6-gingerol and enhancing its therapeutic efficacy.Conclusions: Ginger oil can be prepared as a transdermal drug patch that meets these requirements,and the bioavailability of the prepared patch is better than that of oral administration.It can improve PTSD with good patient compliance and ease of administration.Therefore,it is a promising therapeutic formulation for the treatment of PTSD.展开更多
The use of tissue adhesive patches provides a promising therapeutic approach for sutureless repair of soft tissue injuries.However,existing tissue adhesive patches are confronted with serious challenges for clinical a...The use of tissue adhesive patches provides a promising therapeutic approach for sutureless repair of soft tissue injuries.However,existing tissue adhesive patches are confronted with serious challenges for clinical applications in the soft tissue environments with biological fluids and dynamic movements.Either their mechanical toughness does not match that of soft tissues,or they fail to establish effective interfacial bonding with tissues in wet conditions.The imbalance between the mechanical cohesion and interfacial adhesion of existing tissue adhesive patches severely restricts their conformal integration with wet surfaces of soft tissues in dynamic biological environments,leading to adhesion failure in clinical applications.Here,this study reports the design,fabrication,and preclinical therapeutic performance of a dual-layer silk-based adhesive patch(named SF patch)that quickly and conformally adheres to various soft tissues regardless of surrounding biological environments.The intimate microscopic structural connection between the highly tough hydrogel matrix layer and thin bioadhesive layer contributes to high mechanical cohesion and robust interfacial adhesion properties of the SF patch,thereby enabling sufficient integration with wet surfaces of soft tissues to withstand the interference of dynamic tissue movements.Ex vivo porcine and in vivo rat models validate its therapeutic efficacy for sutureless sealing and repair of gastrointestinal defects and peripheral nerve injuries.This SF patch is potentially valuable for clinical applications towards internal soft-tissue repair and functional reconstruction.展开更多
Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction(MI).However,the low retention of cells and drugs limits the...Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction(MI).However,the low retention of cells and drugs limits their therapeutic efficacies.Here,we propose a prefixed sponge carpet strategy,that is,aldehyde-dextran sponge(ODS)loading anti-oxidative/autophagy-regulating molecular capsules of 2-hydroxy-β-cyclodextrin@resveratrol(HP-β-CD@Res)is first bonded to the rat’s heart via capillary removal of interfacial water from the tissue surface,and the subsequent Schiff base reaction between the aldehyde groups on ODS and amino groups on myocardium tissue.Then,an aqueous biocompatible hydrazided hyaluronic acid(HHA)solution encapsulating mesenchymal stem cells(MSCs)is impregnated into the anchored carpet to form HHA@ODS@HP-β-CD@Res hydrogel in situ via click reaction,thus prolonging the in vivo retention time of therapeutic drug and cells.Importantly,the HHA added to outer surface consumes the remaining aldehydes to contribute to nonsticky top surface,avoiding adhesion to other tissues.The embedded HP-β-CD@Res molecular capsules with antioxidant and autophagy regulation bioactivities can considerably improve cardiac microenvironment,reduce cardiomyocyte apoptosis,and enhance the survival of transplanted MSCs,thereby promoting cardiac repair by facilitating angiogenesis and reducing cardiac fibrosis.展开更多
Wounds, characterized by the disruption of the continuity of body tissues resulting from external trauma,manifest in diverse types and locations. Although numerous wound dressings are available for various woundscenar...Wounds, characterized by the disruption of the continuity of body tissues resulting from external trauma,manifest in diverse types and locations. Although numerous wound dressings are available for various woundscenarios, it remains challenging to find an integrative wound dressing capable of addressing diverse woundsituations. We focused on utilizing sulfated hyaluronan (sHA), known for its anti-inflammatory properties andcapacity to load cationic drugs. By conjugating catechol groups to sHA (sHA-CA), we achieved several advantagesin wound healing: 1) Fabrication of patches through crosslinking with catechol-modified high-molecularweighthyaluronan (HA(HMW)-CA), 2) Adhesiveness that enabled stable localization, 3) Radical scavenging thatcould synergize with the immunomodulation of sHA. The sHA-CA patches demonstrated therapeutic efficacy inthree distinct murine wound models: diabetic wound, hepatic hemorrhage, and post-surgical adhesion. Collectively,these findings underscore the potential of the sHA-CA patch as a promising candidate for the nextgenerationwound dressing.展开更多
Biomaterial-based drug delivery systems have been developed to expedite cartilage regeneration;however,challenges related to drug recovery,validation,and efficient drug delivery remain.For instance,compound K(CK)is a ...Biomaterial-based drug delivery systems have been developed to expedite cartilage regeneration;however,challenges related to drug recovery,validation,and efficient drug delivery remain.For instance,compound K(CK)is a major metabolite of ginsenosides that is known to protect against joint degeneration by inhibiting the production of inflammatory cytokines and the activation of immune cells.However,its effects on cartilage degradation and tissue regeneration remain unclear.Additionally,tissue-adhesive drug delivery depots that stably adhere to cartilage defects are required for CK delivery.In this study,CK-loaded adhesive patches were reported to seal cartilage defects and deliver CK to defect sites,preventing cartilage degradation and accelerating cartilage tissue regeneration.Adhesive patches are stable and suitable for application in surgical procedures under physiological conditions and show excellent adhesiveness to cartilage surfaces.In addition,there were no significant differences in the adhesive polymeric networks before and after CK loading.CK-loaded hydrocaffeic acid-conjugated chitosan patches significantly inhibited the stimulation of cartilage-degrading enzymes and apoptosis in osteoarthritic cartilage by releasing CK in cartilage defects.Additionally,the NFkB signaling pathway of released CK from the adhesive patches in the treatment of osteoarthritis is revealed.Thus,the CK-loaded adhesive patches are expected to significantly contribute to cartilage regeneration.展开更多
The purpose of this study was to investigate the effect of isopropyl myristate (IPM), a penetration enhancer, on the viscoelasticity and drug release of a drug-in-adhesive transdermal patch containing blonanserin. The...The purpose of this study was to investigate the effect of isopropyl myristate (IPM), a penetration enhancer, on the viscoelasticity and drug release of a drug-in-adhesive transdermal patch containing blonanserin. The patches were prepared with DURO-TAK (R) 87-2287 as a pressure-sensitive adhesive (PSA) containing 5% (w/w) of blonanserin and different concentrations of IPM. An in vitro release experiment was performed and the adhesive performance of the drug-in-adhesive patches with different concentrations of IPM was evaluated by a rolling ball tack test and a shear-adhesion test. The glass transition temperature (T-g) and rheological parameters of the drug-in-adhesive layers were determined to study the effect of IPM on the mechanical properties of the PSA. The results of the in vitro release experiment showed that the release rate of blonanserin increased with an increasing concentration of IPM. The rolling ball tack test and shear-adhesion test showed decreasing values with increasing IPM concentration. The results were interpreted on the basis of the IPM-induced plasticization of the PSA, as evidenced by a depression of the glass transition temperature and a decrease in the elastic modulus. In conclusion, IPM acted as a plasticizer on DURO-TAK (R) 87-2287, and it increased the release of blonanserin and affected the adhesive properties of the PSA. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND展开更多
Biomedical patches have demonstrated value in promoting soft tissue repair or anti-adhesion.Research tendency in this area focuses on developing more controllable patches to meet the complex clinical scenarios.Herein,...Biomedical patches have demonstrated value in promoting soft tissue repair or anti-adhesion.Research tendency in this area focuses on developing more controllable patches to meet the complex clinical scenarios.Herein,inspired by the controllable adhesion of suction cups and the antifouling properties of eyeball surfaces,we propose an anisotropic patch with‘revocable’adhesion mechanisms.For the adhesive-side,the initial adhesion forces mainly rely on suction cup’s physical interactions to allow adequate position adjustment,followed by the reaction of N-hydroxysuccinimide ester group with the tissue for firm covalent bonding.This multi-adhesive mechanism enables the spatiotemporal control of adhesive behavior.In contrast,on the barrier-side,the highly hydrated surface derived from polyethylene glycol and polyvinyl alcohol hydrogels displays no affinity for tissue proteins,thus effectively preventing tissue adhesion.Moreover,the intrinsic pores and charges enable the adsorption of positively charged inflammatory factors,while the loaded drugs can release sustainably.In vivo experiments demonstrate the patch’s strong yet controllable adhesion,effective in reducing inflammation and promoting healing.This innovative design introduces a new paradigm of‘revocable’adhesion,offering significant clinical potential for soft tissue repair and adhesion prevention.展开更多
Postoperative abdominal adhesion is a prevalent issue with high incidence rates,often resulting in complications such as bowel obstruction and infertility.Currently,poly(lactic acid)(PLA)-based anti-adhesion membranes...Postoperative abdominal adhesion is a prevalent issue with high incidence rates,often resulting in complications such as bowel obstruction and infertility.Currently,poly(lactic acid)(PLA)-based anti-adhesion membranes are extensively used for the prevention of abdominal adhesions.However,these membranes necessitate suturing,which increases the risk of secondary injury.In this study,we present a Janus patch with asymmetric adhesion properties designed to prevent postoperative abdominal adhesions.The patch consists of two functional layers:an adhesive layer made of a poly(lactic acid-co-ethylethylene phosphate)copolymer,which achieves tissue adhesion via hydrophilicity,hydrogen bonding,and electrostatic interactions,and a non-adhesive layer composed of electrospun PLA membrane.We characterized various properties of the Janus patch,including its morphology,adhesive properties,and biocompatibility.Adhesive properties tests revealed that the adhesive layer of the Janus patch demonstrated superior adhesive capabilities on various tissues compared to the non-adhesive PLA layer.In vivo experiments indicated that the asymmetric adhesive properties of the Janus patch effectively prevent postoperative abdominal adhesions.This work highlights a promising approach for addressing the challenges associated with adhesion prevention and secondary injuries,paving the way for safer and more effective postoperative care.展开更多
基金supported by the National Key Technologies R&D Program of China(Grant No.2023YFC2415900)the National Natural Science Foundation of China(Grant Nos.62373182 and 52405619)+2 种基金the China Postdoctoral Science Foundation(Grant No.2024M751300)supported by the Shenzhen Science and Technology Program(Grant No.JCYJ20241202125417024)Guangdong Basic and Applied Basic Research Foundation(Grant No.2024A1515011915).
文摘Adhesive patches offer an effective approach for wound closure,making them highly suitable for biomedical applications.However,conventional patches often face limitations such as dual-sided adhesion,lack of shape adaptability,and limited maneuverability,which restrict their applications in deeper tissues.In this paper,we develop a magnetic patch robot(PatchBot),for targeted Janus adhesion with tissues.The PatchBot features a unique triple-layer structure,with adhesive,shape-morphing,and anti-adhesive layers,each fulfilling roles to support targeted attachment,enable shape transformation,and prevent unwanted adhesion to surrounding tissues.The Janus adhesion of the PatchBot was extensively demonstrated across a variety of tissues.A localized near-infrared(NIR)laser irradiation was used to induce programmable shape transformations.Magnetic actuation of the PatchBot for targeted adhesion was successfully demonstrated in ex vivo porcine stomach tissue.NIR light-activated shape-morphing and multimodal magnetic actuation significantly enhance its maneuverability and adaptability in confined in vivo environments while ensuring the structural integrity of the adhesive surface during deployment.This proof-of-concept study demonstrates the feasibility of using PatchBot for targeted wound adhesion,showing its potential for minimally invasive,precision therapies in complex in vivo environments.
基金supported by the National Natural Scientific Foundation(82172186)Beijing Natural Scientific Foundation(L222126).
文摘Objective:To find a viable alternative to reduce the number of doses required for the patients with post-traumatic stress disorder(PTSD),and to improve efficacy and patient compliance.Methods: In this study,we used ginger oil,a phytochemical with potential therapeutic properties,to prepare ginger oil patches.High-performance liquid chromatography(HPLC)was used to quantify the main active component of ginger oil,6-gingerol.Transdermal absorption experiments were conducted to optimize the various pressure-sensitive adhesives and permeation enhancers,including their type and concentration.Subsequently,the ginger oil patches were optimized and subjected to content determination and property evaluations.A PTSD mouse model was established using the foot-shock method.The therapeutic effect of ginger oil patches on PTSD was assessed through pathological sections,behavioral tests,and the evaluation of biomarkers such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),brain-derived neurotrophic factor(BDNF),and melatonin(MT).Results: The results demonstrated that ginger oil patches exerted therapeutic effects against PTSD by inhibiting inflammatory responses and modulating MT and BDNF levels.Pharmacokinetic experiments revealed that ginger oil patches maintained a stable blood drug concentration for at least one day,addressing the rapid metabolism drawback of 6-gingerol and enhancing its therapeutic efficacy.Conclusions: Ginger oil can be prepared as a transdermal drug patch that meets these requirements,and the bioavailability of the prepared patch is better than that of oral administration.It can improve PTSD with good patient compliance and ease of administration.Therefore,it is a promising therapeutic formulation for the treatment of PTSD.
基金supported by the National Natural Science Foundation of China(Grant No.22175038,22027805,22334004,22421002)the National Key Research&Development Program of China(Grant No.2020YFA0709900)the Natural Science Foundation of Fujian Province of China(Grant No.2021J01610).
文摘The use of tissue adhesive patches provides a promising therapeutic approach for sutureless repair of soft tissue injuries.However,existing tissue adhesive patches are confronted with serious challenges for clinical applications in the soft tissue environments with biological fluids and dynamic movements.Either their mechanical toughness does not match that of soft tissues,or they fail to establish effective interfacial bonding with tissues in wet conditions.The imbalance between the mechanical cohesion and interfacial adhesion of existing tissue adhesive patches severely restricts their conformal integration with wet surfaces of soft tissues in dynamic biological environments,leading to adhesion failure in clinical applications.Here,this study reports the design,fabrication,and preclinical therapeutic performance of a dual-layer silk-based adhesive patch(named SF patch)that quickly and conformally adheres to various soft tissues regardless of surrounding biological environments.The intimate microscopic structural connection between the highly tough hydrogel matrix layer and thin bioadhesive layer contributes to high mechanical cohesion and robust interfacial adhesion properties of the SF patch,thereby enabling sufficient integration with wet surfaces of soft tissues to withstand the interference of dynamic tissue movements.Ex vivo porcine and in vivo rat models validate its therapeutic efficacy for sutureless sealing and repair of gastrointestinal defects and peripheral nerve injuries.This SF patch is potentially valuable for clinical applications towards internal soft-tissue repair and functional reconstruction.
基金National Natural Science Foundation of China(Grant No.52233008,51733006)National Key Research and Development Program(Grant No.2018YFA0703100).
文摘Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction(MI).However,the low retention of cells and drugs limits their therapeutic efficacies.Here,we propose a prefixed sponge carpet strategy,that is,aldehyde-dextran sponge(ODS)loading anti-oxidative/autophagy-regulating molecular capsules of 2-hydroxy-β-cyclodextrin@resveratrol(HP-β-CD@Res)is first bonded to the rat’s heart via capillary removal of interfacial water from the tissue surface,and the subsequent Schiff base reaction between the aldehyde groups on ODS and amino groups on myocardium tissue.Then,an aqueous biocompatible hydrazided hyaluronic acid(HHA)solution encapsulating mesenchymal stem cells(MSCs)is impregnated into the anchored carpet to form HHA@ODS@HP-β-CD@Res hydrogel in situ via click reaction,thus prolonging the in vivo retention time of therapeutic drug and cells.Importantly,the HHA added to outer surface consumes the remaining aldehydes to contribute to nonsticky top surface,avoiding adhesion to other tissues.The embedded HP-β-CD@Res molecular capsules with antioxidant and autophagy regulation bioactivities can considerably improve cardiac microenvironment,reduce cardiomyocyte apoptosis,and enhance the survival of transplanted MSCs,thereby promoting cardiac repair by facilitating angiogenesis and reducing cardiac fibrosis.
基金support from the Ministry of Science and ICT of Korea(NRF-2021R1A2C2008821 and 2022H1D3A2A02093385)the Korean Fund for Regenerative Medicine(KFRM)grant funded by the Korean government(21A0301L1-21)The Institute of Engineering Research at Seoul National University provided research facilities,and additional support came from the SNU Engineering-Medicine Collaboration grant.
文摘Wounds, characterized by the disruption of the continuity of body tissues resulting from external trauma,manifest in diverse types and locations. Although numerous wound dressings are available for various woundscenarios, it remains challenging to find an integrative wound dressing capable of addressing diverse woundsituations. We focused on utilizing sulfated hyaluronan (sHA), known for its anti-inflammatory properties andcapacity to load cationic drugs. By conjugating catechol groups to sHA (sHA-CA), we achieved several advantagesin wound healing: 1) Fabrication of patches through crosslinking with catechol-modified high-molecularweighthyaluronan (HA(HMW)-CA), 2) Adhesiveness that enabled stable localization, 3) Radical scavenging thatcould synergize with the immunomodulation of sHA. The sHA-CA patches demonstrated therapeutic efficacy inthree distinct murine wound models: diabetic wound, hepatic hemorrhage, and post-surgical adhesion. Collectively,these findings underscore the potential of the sHA-CA patch as a promising candidate for the nextgenerationwound dressing.
基金supported by a National Research Foundation of Korea(NRF)grant funded by the Korean Government(MSIT)(2022R1A4A1031259 and 2021R1I1A3041149)to E.-J.J.by a Korean Fund for Regenerative Medicine(KFRM)grant funded by the Korean Government(Ministry of Science and ICT,Ministry of Health&Welfare)(22A0103L1)to J.H.R.
文摘Biomaterial-based drug delivery systems have been developed to expedite cartilage regeneration;however,challenges related to drug recovery,validation,and efficient drug delivery remain.For instance,compound K(CK)is a major metabolite of ginsenosides that is known to protect against joint degeneration by inhibiting the production of inflammatory cytokines and the activation of immune cells.However,its effects on cartilage degradation and tissue regeneration remain unclear.Additionally,tissue-adhesive drug delivery depots that stably adhere to cartilage defects are required for CK delivery.In this study,CK-loaded adhesive patches were reported to seal cartilage defects and deliver CK to defect sites,preventing cartilage degradation and accelerating cartilage tissue regeneration.Adhesive patches are stable and suitable for application in surgical procedures under physiological conditions and show excellent adhesiveness to cartilage surfaces.In addition,there were no significant differences in the adhesive polymeric networks before and after CK loading.CK-loaded hydrocaffeic acid-conjugated chitosan patches significantly inhibited the stimulation of cartilage-degrading enzymes and apoptosis in osteoarthritic cartilage by releasing CK in cartilage defects.Additionally,the NFkB signaling pathway of released CK from the adhesive patches in the treatment of osteoarthritis is revealed.Thus,the CK-loaded adhesive patches are expected to significantly contribute to cartilage regeneration.
文摘The purpose of this study was to investigate the effect of isopropyl myristate (IPM), a penetration enhancer, on the viscoelasticity and drug release of a drug-in-adhesive transdermal patch containing blonanserin. The patches were prepared with DURO-TAK (R) 87-2287 as a pressure-sensitive adhesive (PSA) containing 5% (w/w) of blonanserin and different concentrations of IPM. An in vitro release experiment was performed and the adhesive performance of the drug-in-adhesive patches with different concentrations of IPM was evaluated by a rolling ball tack test and a shear-adhesion test. The glass transition temperature (T-g) and rheological parameters of the drug-in-adhesive layers were determined to study the effect of IPM on the mechanical properties of the PSA. The results of the in vitro release experiment showed that the release rate of blonanserin increased with an increasing concentration of IPM. The rolling ball tack test and shear-adhesion test showed decreasing values with increasing IPM concentration. The results were interpreted on the basis of the IPM-induced plasticization of the PSA, as evidenced by a depression of the glass transition temperature and a decrease in the elastic modulus. In conclusion, IPM acted as a plasticizer on DURO-TAK (R) 87-2287, and it increased the release of blonanserin and affected the adhesive properties of the PSA. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND
基金The National Natural Science Foundation of China(52403189)the Natural Science Foundation of Jiangsu Province(BK20220170)+1 种基金Shenzhen Second People’s Hospital Clinical Research Fund of Shenzhen High-level Hospital Construction Project(Grant No.20243357016)the Wenzhou Institute UCAS startup fund(WIUCASQD2024009).
文摘Biomedical patches have demonstrated value in promoting soft tissue repair or anti-adhesion.Research tendency in this area focuses on developing more controllable patches to meet the complex clinical scenarios.Herein,inspired by the controllable adhesion of suction cups and the antifouling properties of eyeball surfaces,we propose an anisotropic patch with‘revocable’adhesion mechanisms.For the adhesive-side,the initial adhesion forces mainly rely on suction cup’s physical interactions to allow adequate position adjustment,followed by the reaction of N-hydroxysuccinimide ester group with the tissue for firm covalent bonding.This multi-adhesive mechanism enables the spatiotemporal control of adhesive behavior.In contrast,on the barrier-side,the highly hydrated surface derived from polyethylene glycol and polyvinyl alcohol hydrogels displays no affinity for tissue proteins,thus effectively preventing tissue adhesion.Moreover,the intrinsic pores and charges enable the adsorption of positively charged inflammatory factors,while the loaded drugs can release sustainably.In vivo experiments demonstrate the patch’s strong yet controllable adhesion,effective in reducing inflammation and promoting healing.This innovative design introduces a new paradigm of‘revocable’adhesion,offering significant clinical potential for soft tissue repair and adhesion prevention.
基金supported by the National Key R&D Program of China(2022YFB3804700)the National Natural Science Foundation of China(52373135,52130301).
文摘Postoperative abdominal adhesion is a prevalent issue with high incidence rates,often resulting in complications such as bowel obstruction and infertility.Currently,poly(lactic acid)(PLA)-based anti-adhesion membranes are extensively used for the prevention of abdominal adhesions.However,these membranes necessitate suturing,which increases the risk of secondary injury.In this study,we present a Janus patch with asymmetric adhesion properties designed to prevent postoperative abdominal adhesions.The patch consists of two functional layers:an adhesive layer made of a poly(lactic acid-co-ethylethylene phosphate)copolymer,which achieves tissue adhesion via hydrophilicity,hydrogen bonding,and electrostatic interactions,and a non-adhesive layer composed of electrospun PLA membrane.We characterized various properties of the Janus patch,including its morphology,adhesive properties,and biocompatibility.Adhesive properties tests revealed that the adhesive layer of the Janus patch demonstrated superior adhesive capabilities on various tissues compared to the non-adhesive PLA layer.In vivo experiments indicated that the asymmetric adhesive properties of the Janus patch effectively prevent postoperative abdominal adhesions.This work highlights a promising approach for addressing the challenges associated with adhesion prevention and secondary injuries,paving the way for safer and more effective postoperative care.
