Biomass is a key factor in fermentation process, directly influencing the performance of the fermenta- tion system as well as the quality and yield of the targeted product. Therefore, the on-line estimation of biomass...Biomass is a key factor in fermentation process, directly influencing the performance of the fermenta- tion system as well as the quality and yield of the targeted product. Therefore, the on-line estimation of biomass is indispensable. The soft-sensor based on support vector machine (SVM) for an on-line biomass estimation was ana- lyzed in detail, and the improved SVM called the weighted least squares support vector machine was presented to follow the dynamic feature of fermentation process. The model based on the modified SVM was developed and demonstrated using simulation experiments.展开更多
Minutiae-based fingerprint matching is the most commonly used in an automatic fingerprint identification system. In this paper, we propose a minutia matching method based on line segment vector. This method uses all t...Minutiae-based fingerprint matching is the most commonly used in an automatic fingerprint identification system. In this paper, we propose a minutia matching method based on line segment vector. This method uses all the detected minutiae （the ridge ending and the ridge bifurcation） in a fingerprint image to create a set of new vectors （line segment vector）. Using these vectors, we can determine a truer reference point more efficiently. In addition, this new minutiae vector can also increase the accuracy of the minutiae matching. By experiment on the public domain collections of fingerprint images fvc2004 DID set A and DB4 set A, the result shows that our algorithm can obtain an improved verification performance.展开更多
This paper proposed an optimal algorithm using the sun line-of-sight vector to improve the probe attitude estimation accuracy in deep-space mission.Firstly,the elaborate analysis of the attitude estimation error from ...This paper proposed an optimal algorithm using the sun line-of-sight vector to improve the probe attitude estimation accuracy in deep-space mission.Firstly,the elaborate analysis of the attitude estimation error from vector observations was done to demonstrate that the geometric relation between the reference vectors is an important factor which influences the accuracy of attitude estimation.Then,with introduction of the sun line-of-sight vector,the attitude quaternion obtained from the star-sensor was converted into a pair of mutually perpendicular reference vectors perpendicular to the sun vector.The normalized weights were calculated according to the accuracy of the sensors.Furthermore,the optimal attitude estimation in the least squares sense was achieved with the quaternion estimation method.Finally,the results of simulation demonstrated the validity of the proposed optimal algorithm based on the practical data of the Deep Impact mission.展开更多
On-line chatter detection can avoid unstable cutting through monitoring the machining process.In order to identify chatter in a timely manner,an improved Support Vector Machine(SVM)is developed in this paper,based on ...On-line chatter detection can avoid unstable cutting through monitoring the machining process.In order to identify chatter in a timely manner,an improved Support Vector Machine(SVM)is developed in this paper,based on extracted features.In the SVM model,the penalty factor(e)and the core parameter(g)have important influence on the classification,more than from Kernel Functions(KFs).Hence,first the classification results are conducted using different KFs.Then two methods are presented for exploring the best parameters.The chatter identification results show that the Genetic Algorithm(GA)approach is more suitable for deciding the parameters than the Grid Explore(GE)approach.展开更多
This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with...This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with the new concept of magnetic equipotential surface to magnetic field curves around crossing wires. Cases of single, double, and triple wires are described. Subsequent article will be conducted for more general scenarios where wires are neither infinite nor parallel.展开更多
BACKGROUND Previously,we have successfully constructed replication-competent hepatitis B virus(HBV)vectors by uncoupling the P open reading frame(ORF)from the preC/C ORF to carefully design the transgene insertion sit...BACKGROUND Previously,we have successfully constructed replication-competent hepatitis B virus(HBV)vectors by uncoupling the P open reading frame(ORF)from the preC/C ORF to carefully design the transgene insertion site to overcome the compact organization of the HBV genome and maintain HBV replication competence.Consequently,the replication-competent HBV vectors carrying foreign genes,including pCH-BsdR,carrying blasticidin resistance gene(399 bp),and pCH-hrGFP,carrying humanized renilla green fluorescent protein gene(720 bp),were successfully obtained.However,the replication efficiency of the former is higher but it is tedious to use,while that of the latter is poor and cannot be quantified.Hence,we need to search for a new reporter gene that is convenient and quantifiable for further research.AIM To establish a helpful tool for intracellular HBV replication and anti-viral drugs screening studies.METHODS We utilized the replication-competent HBV viral vectors constructed by our laboratory,combined with the secreted luciferase reporter gene,to construct replication-competent HBV vectors expressing the reporter gene secretory Nanoluc Luciferase(SecNluc).HepG2.TA2-7 cells were transfected with this vector to obtain cell lines with stably secreted HBV particles carrying secNluc reporter gene.RESULTS The replication-competent HBV vector carrying the SecNluc reporter gene pCHsNLuc could produce all major viral RNAs and a full set of envelope proteins and achieve high-level secreted luciferase expression.HBV replication intermediates could be produced from this vector.Via transfection with pTRE-sNLuc and selection by hygromycin,we obtained isolated cell clones,named HBV-NLuc-35 cells,which could secrete secNLuc recombinant viruses,and were sensitive to existing anti-HBV drugs.Using differentiated HepaRG cells,it was verified that recombinant HBV possessed infectivity.CONCLUSION Our research demonstrated that a replication-competent HBV vector carrying a secreted luciferase transgene possesses replication and expression ability,and the established HBV replication and expression cell lines could stably secrete viral particles carrying secNluc reporter gene.More importantly,the cell line and the secreted recombinant viral particles could be used to trace HBV replication or infection.展开更多
针对室内可见光定位中非视距信道(Non Line of Sight,NLOS)导致定位精度不足的问题,提出了一种基于动态高斯加权(Dynamic Gaussian Weighted,DGW)接收信号强度指示(Received Signal Strength Indicator,RSSI)和遗传算法(Genetic Algorit...针对室内可见光定位中非视距信道(Non Line of Sight,NLOS)导致定位精度不足的问题,提出了一种基于动态高斯加权(Dynamic Gaussian Weighted,DGW)接收信号强度指示(Received Signal Strength Indicator,RSSI)和遗传算法(Genetic Algorithm,GA)改进支持向量回归(Support Vector Regression,SVR)的室内可见光定位算法。首先,构建指纹库并划分数据集,计算接收器与光源之间的距离动态调整高斯函数的标准差,再结合RSSI信号的波动性进行自适应加权,以减少NLOS对定位的影响。然后,使用GA优化SVR模型的参数,得到最佳定位模型。最后,使用最佳定位模型对加权后的指纹数据进行定位预测。实验结果表明:本算法的平均定位误差为7.1 cm,相较于SVR、SVR-GA等算法降低了21.1%~42.3%,并且能有效降低NLOS的影响、提高室内定位的精度,具有较强的应用前景。展开更多
【目的】全球离散格网系统(Discrete Global Grid Systems,DGGS)本质上是多尺度栅格结构,地理空间矢量与格网的集成是难点,矢量线格网化是其中的基本问题。现有方案多以平面格网单元中心(格心)连线为矢量线建模结果,但扩展到球面后建模...【目的】全球离散格网系统(Discrete Global Grid Systems,DGGS)本质上是多尺度栅格结构,地理空间矢量与格网的集成是难点,矢量线格网化是其中的基本问题。现有方案多以平面格网单元中心(格心)连线为矢量线建模结果,但扩展到球面后建模精度降低,本文针对这一缺陷提出矢量线全球离散格网系统高精度建模方法。【方法】首先选择与地球拟合程度更高的菱形三十面体构建六边形格网系统,以3个相邻菱形面构成组合结构并建立三轴整数坐标系描述单元空间位置;然后根据矢量线首尾端点所在单元确定最优方向编码以减少搜索范围,通过编码邻近运算搜索矢量线经过的球面单元,以球面格心连线为建模结果并提出跨面矢量线处理方法;最后增加单元顶点(格点)作为结构要素,实现多结构要素矢量线建模,进一步提高建模精度。【结果】实验结果表明:本文方案能正确实现全球各个大洲海岸线格网化建模,确保格网化单元与矢量线拓扑相交,且相较平面格网建模结果兼具精度和效率优势。【结论】针对传统矢量数据格网建模方法的几何精度损失和拓扑畸变问题,本文提出高精度球面格网化建模方法,为矢量数据转换至格网同构处理提供有力支撑。展开更多
基金National Natural Science Foundation of China (No.20476007).
文摘Biomass is a key factor in fermentation process, directly influencing the performance of the fermenta- tion system as well as the quality and yield of the targeted product. Therefore, the on-line estimation of biomass is indispensable. The soft-sensor based on support vector machine (SVM) for an on-line biomass estimation was ana- lyzed in detail, and the improved SVM called the weighted least squares support vector machine was presented to follow the dynamic feature of fermentation process. The model based on the modified SVM was developed and demonstrated using simulation experiments.
文摘Minutiae-based fingerprint matching is the most commonly used in an automatic fingerprint identification system. In this paper, we propose a minutia matching method based on line segment vector. This method uses all the detected minutiae （the ridge ending and the ridge bifurcation） in a fingerprint image to create a set of new vectors （line segment vector）. Using these vectors, we can determine a truer reference point more efficiently. In addition, this new minutiae vector can also increase the accuracy of the minutiae matching. By experiment on the public domain collections of fingerprint images fvc2004 DID set A and DB4 set A, the result shows that our algorithm can obtain an improved verification performance.
文摘This paper proposed an optimal algorithm using the sun line-of-sight vector to improve the probe attitude estimation accuracy in deep-space mission.Firstly,the elaborate analysis of the attitude estimation error from vector observations was done to demonstrate that the geometric relation between the reference vectors is an important factor which influences the accuracy of attitude estimation.Then,with introduction of the sun line-of-sight vector,the attitude quaternion obtained from the star-sensor was converted into a pair of mutually perpendicular reference vectors perpendicular to the sun vector.The normalized weights were calculated according to the accuracy of the sensors.Furthermore,the optimal attitude estimation in the least squares sense was achieved with the quaternion estimation method.Finally,the results of simulation demonstrated the validity of the proposed optimal algorithm based on the practical data of the Deep Impact mission.
文摘On-line chatter detection can avoid unstable cutting through monitoring the machining process.In order to identify chatter in a timely manner,an improved Support Vector Machine(SVM)is developed in this paper,based on extracted features.In the SVM model,the penalty factor(e)and the core parameter(g)have important influence on the classification,more than from Kernel Functions(KFs).Hence,first the classification results are conducted using different KFs.Then two methods are presented for exploring the best parameters.The chatter identification results show that the Genetic Algorithm(GA)approach is more suitable for deciding the parameters than the Grid Explore(GE)approach.
文摘This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with the new concept of magnetic equipotential surface to magnetic field curves around crossing wires. Cases of single, double, and triple wires are described. Subsequent article will be conducted for more general scenarios where wires are neither infinite nor parallel.
基金Supported by the National Natural Science Foundation of China,No.81672041the National Major Science and Technology Special Project for Infectious Diseases of China,No.2012ZX10004503-012
文摘BACKGROUND Previously,we have successfully constructed replication-competent hepatitis B virus(HBV)vectors by uncoupling the P open reading frame(ORF)from the preC/C ORF to carefully design the transgene insertion site to overcome the compact organization of the HBV genome and maintain HBV replication competence.Consequently,the replication-competent HBV vectors carrying foreign genes,including pCH-BsdR,carrying blasticidin resistance gene(399 bp),and pCH-hrGFP,carrying humanized renilla green fluorescent protein gene(720 bp),were successfully obtained.However,the replication efficiency of the former is higher but it is tedious to use,while that of the latter is poor and cannot be quantified.Hence,we need to search for a new reporter gene that is convenient and quantifiable for further research.AIM To establish a helpful tool for intracellular HBV replication and anti-viral drugs screening studies.METHODS We utilized the replication-competent HBV viral vectors constructed by our laboratory,combined with the secreted luciferase reporter gene,to construct replication-competent HBV vectors expressing the reporter gene secretory Nanoluc Luciferase(SecNluc).HepG2.TA2-7 cells were transfected with this vector to obtain cell lines with stably secreted HBV particles carrying secNluc reporter gene.RESULTS The replication-competent HBV vector carrying the SecNluc reporter gene pCHsNLuc could produce all major viral RNAs and a full set of envelope proteins and achieve high-level secreted luciferase expression.HBV replication intermediates could be produced from this vector.Via transfection with pTRE-sNLuc and selection by hygromycin,we obtained isolated cell clones,named HBV-NLuc-35 cells,which could secrete secNLuc recombinant viruses,and were sensitive to existing anti-HBV drugs.Using differentiated HepaRG cells,it was verified that recombinant HBV possessed infectivity.CONCLUSION Our research demonstrated that a replication-competent HBV vector carrying a secreted luciferase transgene possesses replication and expression ability,and the established HBV replication and expression cell lines could stably secrete viral particles carrying secNluc reporter gene.More importantly,the cell line and the secreted recombinant viral particles could be used to trace HBV replication or infection.
文摘针对室内可见光定位中非视距信道(Non Line of Sight,NLOS)导致定位精度不足的问题,提出了一种基于动态高斯加权(Dynamic Gaussian Weighted,DGW)接收信号强度指示(Received Signal Strength Indicator,RSSI)和遗传算法(Genetic Algorithm,GA)改进支持向量回归(Support Vector Regression,SVR)的室内可见光定位算法。首先,构建指纹库并划分数据集,计算接收器与光源之间的距离动态调整高斯函数的标准差,再结合RSSI信号的波动性进行自适应加权,以减少NLOS对定位的影响。然后,使用GA优化SVR模型的参数,得到最佳定位模型。最后,使用最佳定位模型对加权后的指纹数据进行定位预测。实验结果表明:本算法的平均定位误差为7.1 cm,相较于SVR、SVR-GA等算法降低了21.1%~42.3%,并且能有效降低NLOS的影响、提高室内定位的精度,具有较强的应用前景。
文摘【目的】全球离散格网系统(Discrete Global Grid Systems,DGGS)本质上是多尺度栅格结构,地理空间矢量与格网的集成是难点,矢量线格网化是其中的基本问题。现有方案多以平面格网单元中心(格心)连线为矢量线建模结果,但扩展到球面后建模精度降低,本文针对这一缺陷提出矢量线全球离散格网系统高精度建模方法。【方法】首先选择与地球拟合程度更高的菱形三十面体构建六边形格网系统,以3个相邻菱形面构成组合结构并建立三轴整数坐标系描述单元空间位置;然后根据矢量线首尾端点所在单元确定最优方向编码以减少搜索范围,通过编码邻近运算搜索矢量线经过的球面单元,以球面格心连线为建模结果并提出跨面矢量线处理方法;最后增加单元顶点(格点)作为结构要素,实现多结构要素矢量线建模,进一步提高建模精度。【结果】实验结果表明:本文方案能正确实现全球各个大洲海岸线格网化建模,确保格网化单元与矢量线拓扑相交,且相较平面格网建模结果兼具精度和效率优势。【结论】针对传统矢量数据格网建模方法的几何精度损失和拓扑畸变问题,本文提出高精度球面格网化建模方法,为矢量数据转换至格网同构处理提供有力支撑。
