Biomass is a key factor in fermentation process, directly influencing the performance of the fermenta- tion system as well as the quality and yield of the targeted product. Therefore, the on-line estimation of biomass...Biomass is a key factor in fermentation process, directly influencing the performance of the fermenta- tion system as well as the quality and yield of the targeted product. Therefore, the on-line estimation of biomass is indispensable. The soft-sensor based on support vector machine (SVM) for an on-line biomass estimation was ana- lyzed in detail, and the improved SVM called the weighted least squares support vector machine was presented to follow the dynamic feature of fermentation process. The model based on the modified SVM was developed and demonstrated using simulation experiments.展开更多
Minutiae-based fingerprint matching is the most commonly used in an automatic fingerprint identification system. In this paper, we propose a minutia matching method based on line segment vector. This method uses all t...Minutiae-based fingerprint matching is the most commonly used in an automatic fingerprint identification system. In this paper, we propose a minutia matching method based on line segment vector. This method uses all the detected minutiae （the ridge ending and the ridge bifurcation） in a fingerprint image to create a set of new vectors （line segment vector）. Using these vectors, we can determine a truer reference point more efficiently. In addition, this new minutiae vector can also increase the accuracy of the minutiae matching. By experiment on the public domain collections of fingerprint images fvc2004 DID set A and DB4 set A, the result shows that our algorithm can obtain an improved verification performance.展开更多
This paper proposed an optimal algorithm using the sun line-of-sight vector to improve the probe attitude estimation accuracy in deep-space mission.Firstly,the elaborate analysis of the attitude estimation error from ...This paper proposed an optimal algorithm using the sun line-of-sight vector to improve the probe attitude estimation accuracy in deep-space mission.Firstly,the elaborate analysis of the attitude estimation error from vector observations was done to demonstrate that the geometric relation between the reference vectors is an important factor which influences the accuracy of attitude estimation.Then,with introduction of the sun line-of-sight vector,the attitude quaternion obtained from the star-sensor was converted into a pair of mutually perpendicular reference vectors perpendicular to the sun vector.The normalized weights were calculated according to the accuracy of the sensors.Furthermore,the optimal attitude estimation in the least squares sense was achieved with the quaternion estimation method.Finally,the results of simulation demonstrated the validity of the proposed optimal algorithm based on the practical data of the Deep Impact mission.展开更多
On-line chatter detection can avoid unstable cutting through monitoring the machining process.In order to identify chatter in a timely manner,an improved Support Vector Machine(SVM)is developed in this paper,based on ...On-line chatter detection can avoid unstable cutting through monitoring the machining process.In order to identify chatter in a timely manner,an improved Support Vector Machine(SVM)is developed in this paper,based on extracted features.In the SVM model,the penalty factor(e)and the core parameter(g)have important influence on the classification,more than from Kernel Functions(KFs).Hence,first the classification results are conducted using different KFs.Then two methods are presented for exploring the best parameters.The chatter identification results show that the Genetic Algorithm(GA)approach is more suitable for deciding the parameters than the Grid Explore(GE)approach.展开更多
BACKGROUND Previously,we have successfully constructed replication-competent hepatitis B virus(HBV)vectors by uncoupling the P open reading frame(ORF)from the preC/C ORF to carefully design the transgene insertion sit...BACKGROUND Previously,we have successfully constructed replication-competent hepatitis B virus(HBV)vectors by uncoupling the P open reading frame(ORF)from the preC/C ORF to carefully design the transgene insertion site to overcome the compact organization of the HBV genome and maintain HBV replication competence.Consequently,the replication-competent HBV vectors carrying foreign genes,including pCH-BsdR,carrying blasticidin resistance gene(399 bp),and pCH-hrGFP,carrying humanized renilla green fluorescent protein gene(720 bp),were successfully obtained.However,the replication efficiency of the former is higher but it is tedious to use,while that of the latter is poor and cannot be quantified.Hence,we need to search for a new reporter gene that is convenient and quantifiable for further research.AIM To establish a helpful tool for intracellular HBV replication and anti-viral drugs screening studies.METHODS We utilized the replication-competent HBV viral vectors constructed by our laboratory,combined with the secreted luciferase reporter gene,to construct replication-competent HBV vectors expressing the reporter gene secretory Nanoluc Luciferase(SecNluc).HepG2.TA2-7 cells were transfected with this vector to obtain cell lines with stably secreted HBV particles carrying secNluc reporter gene.RESULTS The replication-competent HBV vector carrying the SecNluc reporter gene pCHsNLuc could produce all major viral RNAs and a full set of envelope proteins and achieve high-level secreted luciferase expression.HBV replication intermediates could be produced from this vector.Via transfection with pTRE-sNLuc and selection by hygromycin,we obtained isolated cell clones,named HBV-NLuc-35 cells,which could secrete secNLuc recombinant viruses,and were sensitive to existing anti-HBV drugs.Using differentiated HepaRG cells,it was verified that recombinant HBV possessed infectivity.CONCLUSION Our research demonstrated that a replication-competent HBV vector carrying a secreted luciferase transgene possesses replication and expression ability,and the established HBV replication and expression cell lines could stably secrete viral particles carrying secNluc reporter gene.More importantly,the cell line and the secreted recombinant viral particles could be used to trace HBV replication or infection.展开更多
The general idea in this paper is to study curves of the parametric equations where the parameter varies in a so-called time scale, which may be an arbitrary closed subset of the set of all real numbers. We introduce ...The general idea in this paper is to study curves of the parametric equations where the parameter varies in a so-called time scale, which may be an arbitrary closed subset of the set of all real numbers. We introduce the directional derivative according to the vector fields.展开更多
A set of two-dimensional Stokes spectral data of NOAA AR 10197 obtained by the Solar Stokes Spectral Telescope （S^3T） at the Yunnan Observatory are quafitatively analyzed. The three components of the vector magnetic...A set of two-dimensional Stokes spectral data of NOAA AR 10197 obtained by the Solar Stokes Spectral Telescope （S^3T） at the Yunnan Observatory are quafitatively analyzed. The three components of the vector magnetic field, the strength H, inclination 7 and azimuth X, are derived. Based on the three components, we contour the distributions of the longitudinal magnetic field and transverse magnetic field. The active region （AR） has two different magnetic polarities apparent in the longitudinal magnetic map due to projection effect. There is a basic agreement on the longitudinal magnetic fields between the S^3T and SOHO/MDI magnetograms, with a correlation coefficient PBl = 0.911. The transverse magnetic field of the AR has a radial distribution from a center located in the southwest of the AR. It is also found that the transverse magnetic fields obtained by Huairou Solar Observing Station （HRSOS） have a similar radial distribution. The distributions of transverse magnetic field obtained by S^3T and HRSOS have correlation coefficients, PAzimu = 0.86 and PBt =0.883, in regard to the azimuthal angle and intensity.展开更多
To better understand the effect of a new split variant of human asialoglycoprotein receptor (ASGPR H1b) on ASGPR ligands’ binding ability, we established a functional cell line which expresses ASGPR.The full lengths ...To better understand the effect of a new split variant of human asialoglycoprotein receptor (ASGPR H1b) on ASGPR ligands’ binding ability, we established a functional cell line which expresses ASGPR.The full lengths of ASGPRH1a and H2c fragments from human liver were amplified by reverse transcript PCR (RT-PCR) and inserted into eukaryotic expression vector pIRES2EFP, pCDNA3.1 (Zeo+) respectively.The recombinants were cotransfected into HeLa cells.After selection by using Neocin and Zeocin, a stably transfected cell line was established, which was designated 4-1-6.The transcription and expression of ASGPRH1a and H2c in 4-1-6 were confirmed by RT-PCR, Western blotting and immunofluorescence.The endocytosis function of the artificial "ASGPR" on the surface of 4-1-6 was tested by FACS.It was found that the cell line 4-1-6 could bind ASGPR natural ligand molecular asialo-orosomucoid (ASOR).After the eukaryotic plasmid H1b/pCDNA3.1 (neo) was transfected into cell line 4-1-6, H1b did not down-regulate the ligand binding ability of ASGPR.The eukaryotic expression plasmid H1b/pcDNA3.1 (neo) and H2c/pcDNA3.1 (neo) were co-transfected transiently into Hela cell.Neither single H1b nor H1b and H2c could bind ASOR.In conclusion, a functional cell line of human asialoglycoprotein receptor (ASGPR) which expresses both H1a and H2c stably was established.The new split variant H1b has no effect on ASGPR binding to ASOR.ASGPRH1b alone can’t bind to ASOR, it yet can’t form functional complex with ASGPRH2c.展开更多
Polarization singularities,which emerge from the incoherent superposition of two vector electric fields with the same frequency,and their evolution in free space are studied analytically and illustrated by numerical e...Polarization singularities,which emerge from the incoherent superposition of two vector electric fields with the same frequency,and their evolution in free space are studied analytically and illustrated by numerical examples.It is shown that there exist C-points,L-lines,in particular,C-lines in incoherently superimposed two-dimensional wavefields.Usually,the C-lines are unstable and disappear during the free-space propagation.The motion,pair creation-annihilation process of the emergent C-points,as well as the distortion of the L-lines may take place,and the degree of polarization of the emergent C-points varies upon propagation and may be less than 1.展开更多
Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic ...Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, micro- tubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the su- pernatant were significantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mes- enchymal stem cells. Furthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein743 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic factor/bone marrow mesen- chymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43.展开更多
This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with...This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with the new concept of magnetic equipotential surface to magnetic field curves around crossing wires. Cases of single, double, and triple wires are described. Subsequent article will be conducted for more general scenarios where wires are neither infinite nor parallel.展开更多
基金National Natural Science Foundation of China (No.20476007).
文摘Biomass is a key factor in fermentation process, directly influencing the performance of the fermenta- tion system as well as the quality and yield of the targeted product. Therefore, the on-line estimation of biomass is indispensable. The soft-sensor based on support vector machine (SVM) for an on-line biomass estimation was ana- lyzed in detail, and the improved SVM called the weighted least squares support vector machine was presented to follow the dynamic feature of fermentation process. The model based on the modified SVM was developed and demonstrated using simulation experiments.
文摘Minutiae-based fingerprint matching is the most commonly used in an automatic fingerprint identification system. In this paper, we propose a minutia matching method based on line segment vector. This method uses all the detected minutiae （the ridge ending and the ridge bifurcation） in a fingerprint image to create a set of new vectors （line segment vector）. Using these vectors, we can determine a truer reference point more efficiently. In addition, this new minutiae vector can also increase the accuracy of the minutiae matching. By experiment on the public domain collections of fingerprint images fvc2004 DID set A and DB4 set A, the result shows that our algorithm can obtain an improved verification performance.
文摘This paper proposed an optimal algorithm using the sun line-of-sight vector to improve the probe attitude estimation accuracy in deep-space mission.Firstly,the elaborate analysis of the attitude estimation error from vector observations was done to demonstrate that the geometric relation between the reference vectors is an important factor which influences the accuracy of attitude estimation.Then,with introduction of the sun line-of-sight vector,the attitude quaternion obtained from the star-sensor was converted into a pair of mutually perpendicular reference vectors perpendicular to the sun vector.The normalized weights were calculated according to the accuracy of the sensors.Furthermore,the optimal attitude estimation in the least squares sense was achieved with the quaternion estimation method.Finally,the results of simulation demonstrated the validity of the proposed optimal algorithm based on the practical data of the Deep Impact mission.
文摘On-line chatter detection can avoid unstable cutting through monitoring the machining process.In order to identify chatter in a timely manner,an improved Support Vector Machine(SVM)is developed in this paper,based on extracted features.In the SVM model,the penalty factor(e)and the core parameter(g)have important influence on the classification,more than from Kernel Functions(KFs).Hence,first the classification results are conducted using different KFs.Then two methods are presented for exploring the best parameters.The chatter identification results show that the Genetic Algorithm(GA)approach is more suitable for deciding the parameters than the Grid Explore(GE)approach.
基金Supported by the National Natural Science Foundation of China,No.81672041the National Major Science and Technology Special Project for Infectious Diseases of China,No.2012ZX10004503-012
文摘BACKGROUND Previously,we have successfully constructed replication-competent hepatitis B virus(HBV)vectors by uncoupling the P open reading frame(ORF)from the preC/C ORF to carefully design the transgene insertion site to overcome the compact organization of the HBV genome and maintain HBV replication competence.Consequently,the replication-competent HBV vectors carrying foreign genes,including pCH-BsdR,carrying blasticidin resistance gene(399 bp),and pCH-hrGFP,carrying humanized renilla green fluorescent protein gene(720 bp),were successfully obtained.However,the replication efficiency of the former is higher but it is tedious to use,while that of the latter is poor and cannot be quantified.Hence,we need to search for a new reporter gene that is convenient and quantifiable for further research.AIM To establish a helpful tool for intracellular HBV replication and anti-viral drugs screening studies.METHODS We utilized the replication-competent HBV viral vectors constructed by our laboratory,combined with the secreted luciferase reporter gene,to construct replication-competent HBV vectors expressing the reporter gene secretory Nanoluc Luciferase(SecNluc).HepG2.TA2-7 cells were transfected with this vector to obtain cell lines with stably secreted HBV particles carrying secNluc reporter gene.RESULTS The replication-competent HBV vector carrying the SecNluc reporter gene pCHsNLuc could produce all major viral RNAs and a full set of envelope proteins and achieve high-level secreted luciferase expression.HBV replication intermediates could be produced from this vector.Via transfection with pTRE-sNLuc and selection by hygromycin,we obtained isolated cell clones,named HBV-NLuc-35 cells,which could secrete secNLuc recombinant viruses,and were sensitive to existing anti-HBV drugs.Using differentiated HepaRG cells,it was verified that recombinant HBV possessed infectivity.CONCLUSION Our research demonstrated that a replication-competent HBV vector carrying a secreted luciferase transgene possesses replication and expression ability,and the established HBV replication and expression cell lines could stably secrete viral particles carrying secNluc reporter gene.More importantly,the cell line and the secreted recombinant viral particles could be used to trace HBV replication or infection.
文摘The general idea in this paper is to study curves of the parametric equations where the parameter varies in a so-called time scale, which may be an arbitrary closed subset of the set of all real numbers. We introduce the directional derivative according to the vector fields.
基金Supported by the National Natural Science Foundation of China.
文摘A set of two-dimensional Stokes spectral data of NOAA AR 10197 obtained by the Solar Stokes Spectral Telescope （S^3T） at the Yunnan Observatory are quafitatively analyzed. The three components of the vector magnetic field, the strength H, inclination 7 and azimuth X, are derived. Based on the three components, we contour the distributions of the longitudinal magnetic field and transverse magnetic field. The active region （AR） has two different magnetic polarities apparent in the longitudinal magnetic map due to projection effect. There is a basic agreement on the longitudinal magnetic fields between the S^3T and SOHO/MDI magnetograms, with a correlation coefficient PBl = 0.911. The transverse magnetic field of the AR has a radial distribution from a center located in the southwest of the AR. It is also found that the transverse magnetic fields obtained by Huairou Solar Observing Station （HRSOS） have a similar radial distribution. The distributions of transverse magnetic field obtained by S^3T and HRSOS have correlation coefficients, PAzimu = 0.86 and PBt =0.883, in regard to the azimuthal angle and intensity.
基金supported by grants from the National Major Science and Technology Special Project for Infectious Diseases of China (No.2008ZX10002-011)National High Technology Research and Development of China (Program 863) (No.2006AA02Z128)the National Natural Science Foundation of China (Nos.30700701,30571646)
文摘To better understand the effect of a new split variant of human asialoglycoprotein receptor (ASGPR H1b) on ASGPR ligands’ binding ability, we established a functional cell line which expresses ASGPR.The full lengths of ASGPRH1a and H2c fragments from human liver were amplified by reverse transcript PCR (RT-PCR) and inserted into eukaryotic expression vector pIRES2EFP, pCDNA3.1 (Zeo+) respectively.The recombinants were cotransfected into HeLa cells.After selection by using Neocin and Zeocin, a stably transfected cell line was established, which was designated 4-1-6.The transcription and expression of ASGPRH1a and H2c in 4-1-6 were confirmed by RT-PCR, Western blotting and immunofluorescence.The endocytosis function of the artificial "ASGPR" on the surface of 4-1-6 was tested by FACS.It was found that the cell line 4-1-6 could bind ASGPR natural ligand molecular asialo-orosomucoid (ASOR).After the eukaryotic plasmid H1b/pCDNA3.1 (neo) was transfected into cell line 4-1-6, H1b did not down-regulate the ligand binding ability of ASGPR.The eukaryotic expression plasmid H1b/pcDNA3.1 (neo) and H2c/pcDNA3.1 (neo) were co-transfected transiently into Hela cell.Neither single H1b nor H1b and H2c could bind ASOR.In conclusion, a functional cell line of human asialoglycoprotein receptor (ASGPR) which expresses both H1a and H2c stably was established.The new split variant H1b has no effect on ASGPR binding to ASOR.ASGPRH1b alone can’t bind to ASOR, it yet can’t form functional complex with ASGPRH2c.
基金Project supported by the National Natural Science Foundation of China (Grant No. 10874125)
文摘Polarization singularities,which emerge from the incoherent superposition of two vector electric fields with the same frequency,and their evolution in free space are studied analytically and illustrated by numerical examples.It is shown that there exist C-points,L-lines,in particular,C-lines in incoherently superimposed two-dimensional wavefields.Usually,the C-lines are unstable and disappear during the free-space propagation.The motion,pair creation-annihilation process of the emergent C-points,as well as the distortion of the L-lines may take place,and the degree of polarization of the emergent C-points varies upon propagation and may be less than 1.
文摘Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, micro- tubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the su- pernatant were significantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mes- enchymal stem cells. Furthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein743 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic factor/bone marrow mesen- chymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43.
文摘This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with the new concept of magnetic equipotential surface to magnetic field curves around crossing wires. Cases of single, double, and triple wires are described. Subsequent article will be conducted for more general scenarios where wires are neither infinite nor parallel.
