Castanea sativa is a valuable tree species in Hyrcanian forests, an evolutionary relict ecosystem whose communities suffer from overexploitation and fungal diseases. In the current study, three species delimitation me...Castanea sativa is a valuable tree species in Hyrcanian forests, an evolutionary relict ecosystem whose communities suffer from overexploitation and fungal diseases. In the current study, three species delimitation methods were utilized with ITS regions sequencing to determine the taxonomic status of Septoria causing leaf blotch of C. sativa in Hyrcanian forests. The results indicated that the length of ITS region in the genus Septoria (extracted from GenBank) varied from 650 to 680 bp. There were almost three times more variable sites in ITS1 than in ITS2. The ITS2 secondary structure of Hyrcanian Septoria community had the highest similarity with Septoria castaneicola, except for some differences in helix II and III. Also, Hyrcanian samples had minimum genetic distances with S. castaneicola and maximum with Septoria quercicola. The maximum parsimony method divided the studied Septoria genus into three distinct clades, mostly located in clade I. Clade II consisted entirely of Septoria aciculosa, while clade III contained S. castaneicola as well as Hyrcanian samples.展开更多
北半球针叶林最严重的森林病害是由广义的多年异担子菌(Heterobasidion annosum sensu lato)引起的干基白色腐朽病,但近年的研究表明多年异担子菌并不是单一的物种,目前在欧洲已分出3个独立的种,即原始多年异担子菌(Heterobasidion anno...北半球针叶林最严重的森林病害是由广义的多年异担子菌(Heterobasidion annosum sensu lato)引起的干基白色腐朽病,但近年的研究表明多年异担子菌并不是单一的物种,目前在欧洲已分出3个独立的种,即原始多年异担子菌(Heterobasidion annosum sensu stricto)、小孔异担子菌(Heterobasidion parviporum)和冷杉异担子菌(Heterobasidi-on abietinum),它们生物学习性、形态结构,生态学,寄主范围和发生区域均有差异。应用大伏革菌Phlebiopsisgigantea对该类病害进行生物防治是较好的方法。将中国东北和西南(云南)的异担子菌单孢菌株与欧洲的三种异担子菌进行交配,结果表明中国东北和西南地区的异担子菌与原始多年异担子菌无性亲和反应,而与小孔异担子菌有性融合反应,因此中国东北和西南地区的异担子菌是小孔异担子菌,目前中国并不存在原始多年异担子菌。由于原始多年异担子菌和冷杉异担子菌均为严重的森林病原菌,应将其作为对外检疫对象。展开更多
基金financially supported by Tarbiat Modares University
文摘Castanea sativa is a valuable tree species in Hyrcanian forests, an evolutionary relict ecosystem whose communities suffer from overexploitation and fungal diseases. In the current study, three species delimitation methods were utilized with ITS regions sequencing to determine the taxonomic status of Septoria causing leaf blotch of C. sativa in Hyrcanian forests. The results indicated that the length of ITS region in the genus Septoria (extracted from GenBank) varied from 650 to 680 bp. There were almost three times more variable sites in ITS1 than in ITS2. The ITS2 secondary structure of Hyrcanian Septoria community had the highest similarity with Septoria castaneicola, except for some differences in helix II and III. Also, Hyrcanian samples had minimum genetic distances with S. castaneicola and maximum with Septoria quercicola. The maximum parsimony method divided the studied Septoria genus into three distinct clades, mostly located in clade I. Clade II consisted entirely of Septoria aciculosa, while clade III contained S. castaneicola as well as Hyrcanian samples.
文摘北半球针叶林最严重的森林病害是由广义的多年异担子菌(Heterobasidion annosum sensu lato)引起的干基白色腐朽病,但近年的研究表明多年异担子菌并不是单一的物种,目前在欧洲已分出3个独立的种,即原始多年异担子菌(Heterobasidion annosum sensu stricto)、小孔异担子菌(Heterobasidion parviporum)和冷杉异担子菌(Heterobasidi-on abietinum),它们生物学习性、形态结构,生态学,寄主范围和发生区域均有差异。应用大伏革菌Phlebiopsisgigantea对该类病害进行生物防治是较好的方法。将中国东北和西南(云南)的异担子菌单孢菌株与欧洲的三种异担子菌进行交配,结果表明中国东北和西南地区的异担子菌与原始多年异担子菌无性亲和反应,而与小孔异担子菌有性融合反应,因此中国东北和西南地区的异担子菌是小孔异担子菌,目前中国并不存在原始多年异担子菌。由于原始多年异担子菌和冷杉异担子菌均为严重的森林病原菌,应将其作为对外检疫对象。
