Tyrosinase（TYR） was covalently immobilized onto amino-functionalized carbon felt surface via glutaraldehyde-coupling under ultrasonic treatment for 10 min.The resulting TYR-immobilized carbon felt was used as a work...Tyrosinase（TYR） was covalently immobilized onto amino-functionalized carbon felt surface via glutaraldehyde-coupling under ultrasonic treatment for 10 min.The resulting TYR-immobilized carbon felt was used as a working electrode unit of bioelectrocatalytic flow-through detector for TYR substrates（catechol,p-chlorophenol（p-CP）,p-cresol,phenol etc.）.Cathodic peak currents based on the electroreduction of enzymatically produced o-quinones were detected at-50 mV vs.Ag/AgCl.Compared with previous work in which TYR was immobilized onto amino-functionalized carbon felt for 16 hr without the ultrasonic treatment,we succeeded in（1） shortening the enzyme immobilization time from 16 hr to 10 min,（2） enhancing the sensitivity of p-CP,and（3） improving the operational stability of p-CP.The ultrasonic treatment during the TYR immobilization step would lead to certain changes in the structure of the immobilized TYR and the morphology of the immobilized TYR-layer on the carbon felt surface.展开更多
A novel chemiluminescence(CL) biosensor for the determination of oxalate is presented in this paper. It is based on the CL reaction between luminol, immobilized on an anion-exchange column, and hydrogen peroxide gener...A novel chemiluminescence(CL) biosensor for the determination of oxalate is presented in this paper. It is based on the CL reaction between luminol, immobilized on an anion-exchange column, and hydrogen peroxide generated on-linevia oxalate by the catalytic effect of oxalate oxidase in spinach in the flow cell. The biosensor responds linearly to oxalate concentration in the range of 4 0×10^-7 to 1 0×10^-4 mol/L, and the detection limit is 2×10^-7 mol/L. A complete analysis could be performed in 1 min with a relative standard deviation of 3 7% for 1 0×10^-5 mol/L oxalate(n=7). The system is stable for over 200 times determinations and has been applied in urine samples.展开更多
基金supported in part by the Open Research Center Project of the Ministry of Education,Science and Culture of Japan
文摘Tyrosinase（TYR） was covalently immobilized onto amino-functionalized carbon felt surface via glutaraldehyde-coupling under ultrasonic treatment for 10 min.The resulting TYR-immobilized carbon felt was used as a working electrode unit of bioelectrocatalytic flow-through detector for TYR substrates（catechol,p-chlorophenol（p-CP）,p-cresol,phenol etc.）.Cathodic peak currents based on the electroreduction of enzymatically produced o-quinones were detected at-50 mV vs.Ag/AgCl.Compared with previous work in which TYR was immobilized onto amino-functionalized carbon felt for 16 hr without the ultrasonic treatment,we succeeded in（1） shortening the enzyme immobilization time from 16 hr to 10 min,（2） enhancing the sensitivity of p-CP,and（3） improving the operational stability of p-CP.The ultrasonic treatment during the TYR immobilization step would lead to certain changes in the structure of the immobilized TYR and the morphology of the immobilized TYR-layer on the carbon felt surface.
文摘A novel chemiluminescence(CL) biosensor for the determination of oxalate is presented in this paper. It is based on the CL reaction between luminol, immobilized on an anion-exchange column, and hydrogen peroxide generated on-linevia oxalate by the catalytic effect of oxalate oxidase in spinach in the flow cell. The biosensor responds linearly to oxalate concentration in the range of 4 0×10^-7 to 1 0×10^-4 mol/L, and the detection limit is 2×10^-7 mol/L. A complete analysis could be performed in 1 min with a relative standard deviation of 3 7% for 1 0×10^-5 mol/L oxalate(n=7). The system is stable for over 200 times determinations and has been applied in urine samples.
