Luciferase from firefly lantern extract was immobilized on CNBr activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. The K m′ for D luciferin is 11.9 μmol/L, the ...Luciferase from firefly lantern extract was immobilized on CNBr activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. The K m′ for D luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose bound enzyme were 7.8 and 25℃ respectively. A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak light intensity was linear with respect to ATP concentration in range of 10 -9 -10 -5 mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.展开更多
An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by mi...An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0 2-2 mmol/L and the detection limit was approximately 0 12 mmol/L. The relative standard deviation was 5.3% ( n =6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.展开更多
Fiber-optic DNA biosensors are a kind of ana-lytic setups, which convert the Waston-Crick base pairs matching duplex or Hoogsteen’s tri-plex (T/A-T, C/G-C) formation into a readable analytical signals when functional...Fiber-optic DNA biosensors are a kind of ana-lytic setups, which convert the Waston-Crick base pairs matching duplex or Hoogsteen’s tri-plex (T/A-T, C/G-C) formation into a readable analytical signals when functionalized single- strands DNA (ssDNA) or double-strands DNA (dsDNA) of interest are immobilized on the sur-face of fiber-optic hybrids with target DNA or interacts with ligands. This review will provide the information about the fiber-optic DNA bio-sensors classified into two categories depend-ing on the end fiber and side fiber with or with-out the labels—label-free fiber-optic DNA bio-sensors and labeled fiber-optic DNA biosensor in recent years. Both are dissertated, and em-phasis is on the label-free fiber-optic DNA bio-sensors. Fiber-optic DNA biosensors had got great progresses because fiber-optic has more advantages over the other transducers and are easily processed by nanotechnology. So fiber- optic DNA biosensors have increasingly at-tracted more attention to research and develop the new fiber-optic DNA biosensors that inte-grated with the “nano-bio-info” technology for in vivo test, single molecular detection and on-line medical diagnosis. Finally, future pros-pects to the fiber-optic DNA biosensors are predicted.展开更多
Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An ...Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An operando dual lossy mode resonance(LMR)biosensor fabricated on a D-shaped single-mode fiber(SMF)is proposed for quantification of clinical indicators of inflammatory process,like in COVID-19 infection.Dual LMRs,created via two-step deposition process,yield a nanostructure with distinct SnO_(2) thicknesses on the flat surface of the fiber.Theoretical and experimental analyses confirm its feasibility,showing a sensitivity around 4500 nm/RIU for both LMRs.A novel insight in spatially-separated biofunctionalization of the sensitive fiber regions is validated through fluorescence assays,showcasing selectivity for different immunoglobulins.Real-time and label-free detection of two inflammatory markers,C-reactive protein and Ddimer,empowers the platform capability with a minimum detectable concentration below 1μg/mL for both biomolecules,which is of clinical interest.This proof-of-concept work provides an important leap in fiber-based biosensing for effective and reliable multi-analyte detection,presenting a novel,compact and multi-functional analytical tool.展开更多
In producing high-performance optical biosensors,the selected coupling agent and its fixation mode play an essential role as one of the decisive conditions for antibody incubation.In this work,we designed optical fibe...In producing high-performance optical biosensors,the selected coupling agent and its fixation mode play an essential role as one of the decisive conditions for antibody incubation.In this work,we designed optical fiber biosensors by electrochemical polymerization to enable low detection limit(LOD)immunoassay.Based on the optical fiber lossy mode resonance(OF-LMR)achieved by In_(2)O_(3)-SnO_(2)-90/10 wt%(ITO),we have simultaneously implemented the electropolymerized dopamine(ePDA)film on the ITO-coated fiber via the electrochemical method,utilizing the excellent electrical conductivity of ITO.After that,the immunoglobulin G(IgG)antibody layer was immobilized on the entire sensing region with the assistance of the ePDA film.The results of immunoassay were analyzed by recording the shift of the LMR resonance wavelength to verify the sensor performance.The LOD was evaluated as the lowest concentration of human IgG detected by the OF-LMR sensor,which was confirmed to be 4.20 ng·mL^(-1).Furthermore,the sensor achieved selective detection for specific antigens and exhibited a good recovery capability in chicken serum samples.The developed scheme provides a feasible opportunity to enhance the intersection of electrochemistry and optics subjects and also offers a new promising solution to achieve the immunoassay.展开更多
The ultralow limit of detection(LoD)and exceptional sensitivity of biosensors are a significant challenge currently faced in the field.To address this challenge,this work proposes a highly sensitive laser ring cavity ...The ultralow limit of detection(LoD)and exceptional sensitivity of biosensors are a significant challenge currently faced in the field.To address this challenge,this work proposes a highly sensitive laser ring cavity biosensor capable of detecting low concentrations of des-γ-carboxy prothrombin(DCP).A tapered W-shaped fiber probe based on multi-mode fiber(MMF)-multi-core fiber(MCF)-MMF is developed to excite strong evanescent waves(EWs).By immobilizing gold nanorods(GNRs)on the fiber probe,localized surface plasmon resonance(LSPR)is generated at the near infrared wavelength to further enhance the sensitivity of the fiber probe.Moreover,an erbium-doped fiber(EDF)ring laser with a narrow full width at half maximum(FWHM)of 0.11 nm is employed as a light source.The spectrum with narrow FWHM has been demonstrated to obtain lower Lo D.Compared to the ASE light source,the Lo D of the laser ring cavity can be reduced by an order of magnitude.The developed biosensor is capable of detecting DCP within a concentration range of 0-1000 ng/mL,and the detection sensitivity of 0.265 nm/lg(ng/mL)and the Lo D of 367.6 pg/m L are obtained.In addition,the proposed laser ring cavity biosensor demonstrates good specificity,reproducibility,and repeatability by corresponding tests.The study results indicate that the proposed biosensor has potential in the detection of hepatocellular carcinoma markers.展开更多
A new method of preparing fiber-optic DNA biosensor and its arrayfor the simultaneous detection of multiple genes is described. The optical fibers were first treated with poly-l-lysine, and then were made into fiber-o...A new method of preparing fiber-optic DNA biosensor and its arrayfor the simultaneous detection of multiple genes is described. The optical fibers were first treated with poly-l-lysine, and then were made into fiber-optic DNA biosensors by adsorbing and immobilizing the oligonucleotide probe on its end. By assembling the fiber-optic DNA biosensors in a bundle in which each fiber carried a different DNA probe, the fiber-optic DNA biosensor array was well prepared. Hybridization of fluorescent- labeled cDNA of p53 gene, N-ras gene and Rb1 gene to the DNA array was monitored by CCD camera. A good result was achieved.展开更多
A biosensor featuring an S-tapered fiber(STF) with a composite bio-sensitive film comprising graphene oxide and gold nanoparticles, has been proposed for the rapid, highly sensitive, and label-free detection of pseudo...A biosensor featuring an S-tapered fiber(STF) with a composite bio-sensitive film comprising graphene oxide and gold nanoparticles, has been proposed for the rapid, highly sensitive, and label-free detection of pseudomonas exotoxin(PE).The STF was created using a fusion splicer. Subsequently, the composite film and nanobody were successively assembled onto its surface. The detection mechanism relies on monitoring changes in the external refractive index induced by the specific binding of PE to the nanobody. The developed STF biosensor exhibited a remarkable sensitivity of 0.28 nm/(ng/mL)and a limit of detection as low as 0.21 ng/mL for PE.展开更多
文摘Luciferase from firefly lantern extract was immobilized on CNBr activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. The K m′ for D luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose bound enzyme were 7.8 and 25℃ respectively. A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak light intensity was linear with respect to ATP concentration in range of 10 -9 -10 -5 mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.
文摘An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0 2-2 mmol/L and the detection limit was approximately 0 12 mmol/L. The relative standard deviation was 5.3% ( n =6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.
文摘Fiber-optic DNA biosensors are a kind of ana-lytic setups, which convert the Waston-Crick base pairs matching duplex or Hoogsteen’s tri-plex (T/A-T, C/G-C) formation into a readable analytical signals when functionalized single- strands DNA (ssDNA) or double-strands DNA (dsDNA) of interest are immobilized on the sur-face of fiber-optic hybrids with target DNA or interacts with ligands. This review will provide the information about the fiber-optic DNA bio-sensors classified into two categories depend-ing on the end fiber and side fiber with or with-out the labels—label-free fiber-optic DNA bio-sensors and labeled fiber-optic DNA biosensor in recent years. Both are dissertated, and em-phasis is on the label-free fiber-optic DNA bio-sensors. Fiber-optic DNA biosensors had got great progresses because fiber-optic has more advantages over the other transducers and are easily processed by nanotechnology. So fiber- optic DNA biosensors have increasingly at-tracted more attention to research and develop the new fiber-optic DNA biosensors that inte-grated with the “nano-bio-info” technology for in vivo test, single molecular detection and on-line medical diagnosis. Finally, future pros-pects to the fiber-optic DNA biosensors are predicted.
基金financial support from the Spanish Agencia Estatal de Investigación (AEI) through project PID2023-149895OB-I00a predoctoral research grant from the Public University of Navarrafinancial support under the National Recovery and Resilience Plan (NRRP),Mission 4,Component 2,Investment 1.1,Call for tender No.1409 published on 14.9.2022 by the Italian Ministry of University and Research (MUR),funded by the European Union–NextGenerationEU–Project Title‘‘Fiber optics sensors as a platform for cancer diagnosis and in vitro model testing”–CUP B53D23024170001-Grant Assignment Decree No.1383 adopted on 01/09/2023 by the Italian MUR.
文摘Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An operando dual lossy mode resonance(LMR)biosensor fabricated on a D-shaped single-mode fiber(SMF)is proposed for quantification of clinical indicators of inflammatory process,like in COVID-19 infection.Dual LMRs,created via two-step deposition process,yield a nanostructure with distinct SnO_(2) thicknesses on the flat surface of the fiber.Theoretical and experimental analyses confirm its feasibility,showing a sensitivity around 4500 nm/RIU for both LMRs.A novel insight in spatially-separated biofunctionalization of the sensitive fiber regions is validated through fluorescence assays,showcasing selectivity for different immunoglobulins.Real-time and label-free detection of two inflammatory markers,C-reactive protein and Ddimer,empowers the platform capability with a minimum detectable concentration below 1μg/mL for both biomolecules,which is of clinical interest.This proof-of-concept work provides an important leap in fiber-based biosensing for effective and reliable multi-analyte detection,presenting a novel,compact and multi-functional analytical tool.
基金supported in parts by National Key Scientific Instrument and Equipment Development Projects of China(Grant No.2022YFF0706000)National Natural Science Foundation of China(Grant No.62035006)+1 种基金Independent Innovation Found of Tianjin University,China(Grant No.2023XCG-0028)Tianjin Research Innovation Project for Postgraduate Students,China(Grant No.2022BKY064).
文摘In producing high-performance optical biosensors,the selected coupling agent and its fixation mode play an essential role as one of the decisive conditions for antibody incubation.In this work,we designed optical fiber biosensors by electrochemical polymerization to enable low detection limit(LOD)immunoassay.Based on the optical fiber lossy mode resonance(OF-LMR)achieved by In_(2)O_(3)-SnO_(2)-90/10 wt%(ITO),we have simultaneously implemented the electropolymerized dopamine(ePDA)film on the ITO-coated fiber via the electrochemical method,utilizing the excellent electrical conductivity of ITO.After that,the immunoglobulin G(IgG)antibody layer was immobilized on the entire sensing region with the assistance of the ePDA film.The results of immunoassay were analyzed by recording the shift of the LMR resonance wavelength to verify the sensor performance.The LOD was evaluated as the lowest concentration of human IgG detected by the OF-LMR sensor,which was confirmed to be 4.20 ng·mL^(-1).Furthermore,the sensor achieved selective detection for specific antigens and exhibited a good recovery capability in chicken serum samples.The developed scheme provides a feasible opportunity to enhance the intersection of electrochemistry and optics subjects and also offers a new promising solution to achieve the immunoassay.
基金Natural Science Foundation of Shandong Province(ZR2022QF137)Double-Hundred Talent Plan of Shandong Province+3 种基金Special Construction Project Fund for Shandong Province Taishan Mountain ScholarsLiaocheng University(318052205,318052341)Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China(2022KJ107)Koneru Lakshmaiah Education Foundation。
文摘The ultralow limit of detection(LoD)and exceptional sensitivity of biosensors are a significant challenge currently faced in the field.To address this challenge,this work proposes a highly sensitive laser ring cavity biosensor capable of detecting low concentrations of des-γ-carboxy prothrombin(DCP).A tapered W-shaped fiber probe based on multi-mode fiber(MMF)-multi-core fiber(MCF)-MMF is developed to excite strong evanescent waves(EWs).By immobilizing gold nanorods(GNRs)on the fiber probe,localized surface plasmon resonance(LSPR)is generated at the near infrared wavelength to further enhance the sensitivity of the fiber probe.Moreover,an erbium-doped fiber(EDF)ring laser with a narrow full width at half maximum(FWHM)of 0.11 nm is employed as a light source.The spectrum with narrow FWHM has been demonstrated to obtain lower Lo D.Compared to the ASE light source,the Lo D of the laser ring cavity can be reduced by an order of magnitude.The developed biosensor is capable of detecting DCP within a concentration range of 0-1000 ng/mL,and the detection sensitivity of 0.265 nm/lg(ng/mL)and the Lo D of 367.6 pg/m L are obtained.In addition,the proposed laser ring cavity biosensor demonstrates good specificity,reproducibility,and repeatability by corresponding tests.The study results indicate that the proposed biosensor has potential in the detection of hepatocellular carcinoma markers.
文摘A new method of preparing fiber-optic DNA biosensor and its arrayfor the simultaneous detection of multiple genes is described. The optical fibers were first treated with poly-l-lysine, and then were made into fiber-optic DNA biosensors by adsorbing and immobilizing the oligonucleotide probe on its end. By assembling the fiber-optic DNA biosensors in a bundle in which each fiber carried a different DNA probe, the fiber-optic DNA biosensor array was well prepared. Hybridization of fluorescent- labeled cDNA of p53 gene, N-ras gene and Rb1 gene to the DNA array was monitored by CCD camera. A good result was achieved.
基金supported by the National Natural Science Foundation of China (Nos.91860140,62090064,and U19A2077)。
文摘A biosensor featuring an S-tapered fiber(STF) with a composite bio-sensitive film comprising graphene oxide and gold nanoparticles, has been proposed for the rapid, highly sensitive, and label-free detection of pseudomonas exotoxin(PE).The STF was created using a fusion splicer. Subsequently, the composite film and nanobody were successively assembled onto its surface. The detection mechanism relies on monitoring changes in the external refractive index induced by the specific binding of PE to the nanobody. The developed STF biosensor exhibited a remarkable sensitivity of 0.28 nm/(ng/mL)and a limit of detection as low as 0.21 ng/mL for PE.
