Adosomus sp. eats the leaves and oviposits on the stems ofArtemisia ordosica Krasch. Based on an analytical PTI-GC/MS method, 21 volatile organic compounds (VOCs) from the leaves ofA. ordosica were identified. The m...Adosomus sp. eats the leaves and oviposits on the stems ofArtemisia ordosica Krasch. Based on an analytical PTI-GC/MS method, 21 volatile organic compounds (VOCs) from the leaves ofA. ordosica were identified. The major operating equipment consisted of a DB-5 column (30 m × 0.32 mm × 0.25 μm) and a flame ionization detector (FID). The amounts of the components from VOCs were determined by a peak area normalization method and 17 VOC components were identified by PTI-GC/MS. The elec- troantennogram (EAG) responses ofAdosomus sp. to 17 of the VOCs were tested. The results show that seven compounds, i.e., oci- mene, myrcene, R-(+)-α-pinene, caryophyllene, S-(+)-2-canene, humulene and (+)limonene elicited strong EAG responses by female Adosomus sp., with myrcene and ocimene eliciting the strongest responses. It is interesting to note that male Adosomus sp. presented different EAG responses from females to those compounds. Four volatile compounds: β-pinene, myrcene, trans-ocimene and longipinene elicited strong EAG responses to males with myrcene eliciting the strongest response.展开更多
Based on an analytical TCT-GC/MS method, volatile compounds of branches of Platycladus orientalis under three physiological states (healthy, weak and baited trees) were analyzed. The results showed that α-pinene, ...Based on an analytical TCT-GC/MS method, volatile compounds of branches of Platycladus orientalis under three physiological states (healthy, weak and baited trees) were analyzed. The results showed that α-pinene, β-phellandrene, 3-careen, limonene and thujopsene were released under all three physiological states. There were other kinds of volatiles released from branches of weak P. orientalis trees, such as camphene, γ-terpinene, (+)-4-carene and cedrene, but these four volatiles compounds were not released from the branches of healthy P. orientalis. Volatile components from the physically damaged branches of P. orientalis had changed. β-terpinene, mycrene, isocaryophillene and caryophyllene were identified in both healthy and weak branches of P. orientalis. Only cedrene was identified in the baited wood. Electroantennogram (EAG) tests were conducted with nine volatile compounds from the branches of P. orientalis. Antennnae of male and female Semanotus bifasciatus (Motschulsky) were highly sensitive to these nine volatile compounds. Cedrene elicited the strongest response, whereas β-terpinene the lowest. Males and females showed no signifi-cant difference in their EAG responses to the nine volatile compounds.展开更多
Electrophysiological and behavioral responses of adult A. glabripennis (Motsch.) to volatiles from ashleaf maple (Acer negundo L.) were investigated to identify semiochemicals involved in host location. Measurable ele...Electrophysiological and behavioral responses of adult A. glabripennis (Motsch.) to volatiles from ashleaf maple (Acer negundo L.) were investigated to identify semiochemicals involved in host location. Measurable electroantennogram (BAG) responses were elicited to all compounds tested, the most effective antennal stimulants were trans-2-hexen-1-al,decyl aldehyde and trans-2-hexenl-ol.These profiles were similar between males and females. In Y-tube olfactometer bioassays, above three compounds with certain concentration, trans-2-hexen-l-al (1%), trans-2-hexen-l-ol (1%) and decyl aldehyde (10%), were significantly attractive to the adults in laboratory. The results show that either BAG or olfactory responding to a particular volatile compound are markedly influenced by the concentration.展开更多
【目的】枣飞象Scythropus yasumatsui Kono et Morimoto是枣树重要害虫之一。近年来,在陕北黄河沿岸枣区暴发成灾,造成了严重的经济和生态损失。为了明确枣树挥发物在枣飞象寄主定向过程中的作用,以便为开发植物源引诱剂奠定理论基础...【目的】枣飞象Scythropus yasumatsui Kono et Morimoto是枣树重要害虫之一。近年来,在陕北黄河沿岸枣区暴发成灾,造成了严重的经济和生态损失。为了明确枣树挥发物在枣飞象寄主定向过程中的作用,以便为开发植物源引诱剂奠定理论基础。【方法】采用触角电位仪和Y型嗅觉仪测定了枣飞象对枣树7种挥发物的触角电位和嗅觉行为反应。【结果】EAG试验结果表明:枣树的7种挥发物在一定的浓度下,都能引起枣飞象雌雄成虫明显触角电位反应,当刺激浓度为50μg/μL时,枣飞象对测定的7种挥发物的触角电位值达到最高,其中枣飞象雌雄虫对罗勒烯的EAG反应相对值均为最大,分别达2.87和2.53。嗅觉行为反应试验结果表明:罗勒烯、α-法呢烯均对枣飞象雌雄虫有明显的引诱作用,其中枣飞象雌雄成虫对罗勒烯趋向率高达70.8%、70.4%,而反-2-己烯醇仅对其雌虫表现引诱作用,对雄虫未表现出引诱作用。【结论】罗勒烯和α-法呢烯为吸引枣飞象雌雄成虫的活性物质,本反-2-己烯醇为吸引枣飞象雌虫的活性物质。展开更多
基金supported by the technology pillar program in the "Eleventh Five-year" National Science and Technology Support Program of China (2006BA-D08A10)the program for Changjiang Scholars and Innovative Research Teams in Universities (PC-SIRT0607)
文摘Adosomus sp. eats the leaves and oviposits on the stems ofArtemisia ordosica Krasch. Based on an analytical PTI-GC/MS method, 21 volatile organic compounds (VOCs) from the leaves ofA. ordosica were identified. The major operating equipment consisted of a DB-5 column (30 m × 0.32 mm × 0.25 μm) and a flame ionization detector (FID). The amounts of the components from VOCs were determined by a peak area normalization method and 17 VOC components were identified by PTI-GC/MS. The elec- troantennogram (EAG) responses ofAdosomus sp. to 17 of the VOCs were tested. The results show that seven compounds, i.e., oci- mene, myrcene, R-(+)-α-pinene, caryophyllene, S-(+)-2-canene, humulene and (+)limonene elicited strong EAG responses by female Adosomus sp., with myrcene and ocimene eliciting the strongest responses. It is interesting to note that male Adosomus sp. presented different EAG responses from females to those compounds. Four volatile compounds: β-pinene, myrcene, trans-ocimene and longipinene elicited strong EAG responses to males with myrcene eliciting the strongest response.
文摘Based on an analytical TCT-GC/MS method, volatile compounds of branches of Platycladus orientalis under three physiological states (healthy, weak and baited trees) were analyzed. The results showed that α-pinene, β-phellandrene, 3-careen, limonene and thujopsene were released under all three physiological states. There were other kinds of volatiles released from branches of weak P. orientalis trees, such as camphene, γ-terpinene, (+)-4-carene and cedrene, but these four volatiles compounds were not released from the branches of healthy P. orientalis. Volatile components from the physically damaged branches of P. orientalis had changed. β-terpinene, mycrene, isocaryophillene and caryophyllene were identified in both healthy and weak branches of P. orientalis. Only cedrene was identified in the baited wood. Electroantennogram (EAG) tests were conducted with nine volatile compounds from the branches of P. orientalis. Antennnae of male and female Semanotus bifasciatus (Motschulsky) were highly sensitive to these nine volatile compounds. Cedrene elicited the strongest response, whereas β-terpinene the lowest. Males and females showed no signifi-cant difference in their EAG responses to the nine volatile compounds.
基金Fund of China's National "863" Project(Grant No.2001AA249071)the"Tenth-Five-Year Plan"National Key Project(Grant No.2001BA509B0903)
文摘Electrophysiological and behavioral responses of adult A. glabripennis (Motsch.) to volatiles from ashleaf maple (Acer negundo L.) were investigated to identify semiochemicals involved in host location. Measurable electroantennogram (BAG) responses were elicited to all compounds tested, the most effective antennal stimulants were trans-2-hexen-1-al,decyl aldehyde and trans-2-hexenl-ol.These profiles were similar between males and females. In Y-tube olfactometer bioassays, above three compounds with certain concentration, trans-2-hexen-l-al (1%), trans-2-hexen-l-ol (1%) and decyl aldehyde (10%), were significantly attractive to the adults in laboratory. The results show that either BAG or olfactory responding to a particular volatile compound are markedly influenced by the concentration.
文摘【目的】枣飞象Scythropus yasumatsui Kono et Morimoto是枣树重要害虫之一。近年来,在陕北黄河沿岸枣区暴发成灾,造成了严重的经济和生态损失。为了明确枣树挥发物在枣飞象寄主定向过程中的作用,以便为开发植物源引诱剂奠定理论基础。【方法】采用触角电位仪和Y型嗅觉仪测定了枣飞象对枣树7种挥发物的触角电位和嗅觉行为反应。【结果】EAG试验结果表明:枣树的7种挥发物在一定的浓度下,都能引起枣飞象雌雄成虫明显触角电位反应,当刺激浓度为50μg/μL时,枣飞象对测定的7种挥发物的触角电位值达到最高,其中枣飞象雌雄虫对罗勒烯的EAG反应相对值均为最大,分别达2.87和2.53。嗅觉行为反应试验结果表明:罗勒烯、α-法呢烯均对枣飞象雌雄虫有明显的引诱作用,其中枣飞象雌雄成虫对罗勒烯趋向率高达70.8%、70.4%,而反-2-己烯醇仅对其雌虫表现引诱作用,对雄虫未表现出引诱作用。【结论】罗勒烯和α-法呢烯为吸引枣飞象雌雄成虫的活性物质,本反-2-己烯醇为吸引枣飞象雌虫的活性物质。