Osteoporosis(OP)is a noncommunicable bone disease caused by a shift in the balance between os-teoblasts and osteoclasts,and can severely affect the health of elderly persons.Autologous stem-cell transplantation can im...Osteoporosis(OP)is a noncommunicable bone disease caused by a shift in the balance between os-teoblasts and osteoclasts,and can severely affect the health of elderly persons.Autologous stem-cell transplantation can improve reduced bone density and weakened fracture healing abilities in patients with OP.However,OP can adversely affect the osteogenesis and proliferation abilities of autologous adipose-derived stem cells(ASCs).Therefore,an effective drug is required to facilitate autologous ASCs to recover their osteogenic and proliferative potential.Tetrahedral framework nucleic acid(tFNA)is a new type of nanomaterial that has ability to regulate the biological behavior of cells effectively and en-hance the bioactivity of stem cells.In this study,we examine the effects of tFNAs on the osteogenic differentiation and proliferation abilities of ASCs in rats with OP.The results indicate that the 250 nmol/L tFNAs can considerably increase the expression of osteogenesis-related markers,effectively promote the proliferation and osteogenic differentiation of osteoporotic ASCs(OP-ASCs),and help them to regain their osteogenic and proliferative potential.In short,tFNAs can enable OP-ACSs to recover their osteogenic po-tential and promote their proliferation and,therefore,can play a key regulatory role in autologous ASC transplantation.展开更多
The rapidly developing rural tourism industry in China has deeply influenced the livelihoods of rural households. This study compares the impact of rural tourism development in poor areas on the farmers' livelihoo...The rapidly developing rural tourism industry in China has deeply influenced the livelihoods of rural households. This study compares the impact of rural tourism development in poor areas on the farmers' livelihoods, based on the Sustainable Livelihoods Framework proposed by the United Kingdom's Department for International Development. Gougezhuang, a tourist village, and Jiaojiekou, a non-tourist village, both located in Yesanpo Tourism District in the state-level povertystricken Laishui County, Hebei Province, were selected as case studies. The livelihood models of the two villages show great differentiation after 20 years of tourism development. Gougezhuang Village has been driven by the tourism industry and farmers are employed locally, enjoying a relatively higher-income life, with a developmental livelihood model. In contrast, farmers in Jiaojiekou Village earn their living mainly by leaving home to work in the city. They have a low income, with a survival livelihood model, supported by part-time business. Considering that the two villages are adjacent and have similar development backgrounds, the analysis results indicate that rural tourism has an apparent locationspecific effect on poverty alleviation and socioeconomic development, but the development ofrural tourism is limited regarding large-scale poverty alleviation efforts in poor areas. To implement a propoor-tourism strategy and achieve sustainable development, it is necessary to implement comprehensive management measures to change the local conditions of rural settlements and make full use of the spillover effect of tourist consumption.展开更多
We utilized a unique culture system to analyze the expression patterns of gene, protein, and cell surface antigen, and the biological process of the related genes in erythroid and myeloid differentiation and switching...We utilized a unique culture system to analyze the expression patterns of gene, protein, and cell surface antigen, and the biological process of the related genes in erythroid and myeloid differentiation and switching of hematopoietic stem cells （HSCs） in response to cytokine alterations. Gene-specific fragments （266） identified from five populations of cytokine-stimulated HSCs were categorized into three groups： （1） expressed specifically in a single cell population; （2） expressed in two cell populations, and （3） expressed in three or more populations. Of 145 defined cDNAs, three （2%） were novel genes. Protein two-dimensional gel electrophoresis and flow cytometry analyses showed overlapped and distinguished protein expression profiles in the cell populations studied. Biological process mapping of mRNAs expressed in erythroid and myeloid lineages indicated that mRNAs shared by both lineages attended ＇core processes,＇ whereas genes specifically expressed in either lineage alone were related to specific processes or cellular maturation. Data from this study support the hypothesis that committed HSCs （El4 or G14） cells can still be redirected to develop into myeloid or erythroid cells when erythropoietin （EPO） is replaced with granulocyte-colony stimulating factor （G-CSF） under erythroid-cultured condition or G-CSF with EPO in myeloid-cultured environment, respectively. Our results suggest that genes or proteins co-expressed in erythroid and myeloid lineages may be essential for the lineage maintenance and switching in hematopoiesis.展开更多
Machine learning(ML)surrogate models offer a promising route to accelerate material property prediction,bypassing costly atomistic simulations.Here,we introduce IsothermODE,a neural ordinary differential equation(NODE...Machine learning(ML)surrogate models offer a promising route to accelerate material property prediction,bypassing costly atomistic simulations.Here,we introduce IsothermODE,a neural ordinary differential equation(NODE)framework for reconstructing full uptake and heat of adsorption(ΔH_(ads))isotherms for CO_(2)adsorption in metal-organic frameworks(MOFs)using only sparse pressure data.Unlike traditional ML models,IsothermODE leverages the intrinsic structure of differential equations to produce smooth,physically-consistent predictions that generalize across wide pressure ranges.We demonstrate high-fidelity interpolation and extrapolation,even with only five pressure points.To address the stochasticity inherent in Grand Canonical Monte Carlo(GCMC)simulations,we integrate uncertainty quantification,yielding tight bounds on predicted enthalpy curves.We further interpret the learned latent dynamics in terms of adsorption thermodynamics and textural properties,offering insight into structure-property relationships.Finally,we demonstrate IsothermODE’s long-range interpolation and extrapolation capabilities with sparse isotherm data(5 pressure points)and large incomplete intervals featuring missing data between 4–40(case 1)and 25–50(case 2)bars.IsothermODE provides a fast,robust alternative to simulation-heavy workflows,enabling scalable screening and design of next-generation carbon capture materials.展开更多
探讨基于广义语言障碍临床思考框架建立的“语言-社交沟通-认知”三维度儿童语言评估与干预模式。首先,梳理神经发育性障碍儿童的常见语言与沟通问题,列举了符合三维度模式的循证早期语言干预体系。随着儿童进入学龄前期和学龄期,在鉴...探讨基于广义语言障碍临床思考框架建立的“语言-社交沟通-认知”三维度儿童语言评估与干预模式。首先,梳理神经发育性障碍儿童的常见语言与沟通问题,列举了符合三维度模式的循证早期语言干预体系。随着儿童进入学龄前期和学龄期,在鉴别诊断的过程中通过此模型分析出语言障碍、孤独症谱系障碍、注意缺陷多动障碍、智力障碍等不同根源问题。早期语言干预体系和单线的语言干预流程都不能有效推进儿童在学校学习和社交所需的关键技能。本文强调了三维模型中的“心智解读”(Theory of Mind)能力在这一阶段的重要作用,并专设章节进行详细阐述。最后,提出了将“语言-社交沟通-认知”三维度模式落实于实践的基于叙事的,三个维度协同推进的语言干预体系,并阐述了其循证基础和临床流程。展开更多
基金supported by the National Key R&D Program of China(No.2019YFA0110600)National Natural Science Founda-tion of China(Nos.82171006,81970986,81771125,82001432)+1 种基金China Postdoctoral Science Foundation(Nos.2020TQ0213,2020M683319)West China Hospital Postdoctoral Science Foundation(No.2020HXBH104).
文摘Osteoporosis(OP)is a noncommunicable bone disease caused by a shift in the balance between os-teoblasts and osteoclasts,and can severely affect the health of elderly persons.Autologous stem-cell transplantation can improve reduced bone density and weakened fracture healing abilities in patients with OP.However,OP can adversely affect the osteogenesis and proliferation abilities of autologous adipose-derived stem cells(ASCs).Therefore,an effective drug is required to facilitate autologous ASCs to recover their osteogenic and proliferative potential.Tetrahedral framework nucleic acid(tFNA)is a new type of nanomaterial that has ability to regulate the biological behavior of cells effectively and en-hance the bioactivity of stem cells.In this study,we examine the effects of tFNAs on the osteogenic differentiation and proliferation abilities of ASCs in rats with OP.The results indicate that the 250 nmol/L tFNAs can considerably increase the expression of osteogenesis-related markers,effectively promote the proliferation and osteogenic differentiation of osteoporotic ASCs(OP-ASCs),and help them to regain their osteogenic and proliferative potential.In short,tFNAs can enable OP-ACSs to recover their osteogenic po-tential and promote their proliferation and,therefore,can play a key regulatory role in autologous ASC transplantation.
基金supported by the National Natural Science Foundation of China (Grant no. 41671151)the National Social Science Foundation of China (Grant no. 17BGL123)+1 种基金the Key Project of China National Tourism Administration Research Foundation (Grant no. 16TAAK004)the Tourism Young Expert Training Program of China National Tourism Administration (TYETP201527)
文摘The rapidly developing rural tourism industry in China has deeply influenced the livelihoods of rural households. This study compares the impact of rural tourism development in poor areas on the farmers' livelihoods, based on the Sustainable Livelihoods Framework proposed by the United Kingdom's Department for International Development. Gougezhuang, a tourist village, and Jiaojiekou, a non-tourist village, both located in Yesanpo Tourism District in the state-level povertystricken Laishui County, Hebei Province, were selected as case studies. The livelihood models of the two villages show great differentiation after 20 years of tourism development. Gougezhuang Village has been driven by the tourism industry and farmers are employed locally, enjoying a relatively higher-income life, with a developmental livelihood model. In contrast, farmers in Jiaojiekou Village earn their living mainly by leaving home to work in the city. They have a low income, with a survival livelihood model, supported by part-time business. Considering that the two villages are adjacent and have similar development backgrounds, the analysis results indicate that rural tourism has an apparent locationspecific effect on poverty alleviation and socioeconomic development, but the development ofrural tourism is limited regarding large-scale poverty alleviation efforts in poor areas. To implement a propoor-tourism strategy and achieve sustainable development, it is necessary to implement comprehensive management measures to change the local conditions of rural settlements and make full use of the spillover effect of tourist consumption.
文摘We utilized a unique culture system to analyze the expression patterns of gene, protein, and cell surface antigen, and the biological process of the related genes in erythroid and myeloid differentiation and switching of hematopoietic stem cells （HSCs） in response to cytokine alterations. Gene-specific fragments （266） identified from five populations of cytokine-stimulated HSCs were categorized into three groups： （1） expressed specifically in a single cell population; （2） expressed in two cell populations, and （3） expressed in three or more populations. Of 145 defined cDNAs, three （2%） were novel genes. Protein two-dimensional gel electrophoresis and flow cytometry analyses showed overlapped and distinguished protein expression profiles in the cell populations studied. Biological process mapping of mRNAs expressed in erythroid and myeloid lineages indicated that mRNAs shared by both lineages attended ＇core processes,＇ whereas genes specifically expressed in either lineage alone were related to specific processes or cellular maturation. Data from this study support the hypothesis that committed HSCs （El4 or G14） cells can still be redirected to develop into myeloid or erythroid cells when erythropoietin （EPO） is replaced with granulocyte-colony stimulating factor （G-CSF） under erythroid-cultured condition or G-CSF with EPO in myeloid-cultured environment, respectively. Our results suggest that genes or proteins co-expressed in erythroid and myeloid lineages may be essential for the lineage maintenance and switching in hematopoiesis.
基金the Engaging OnDemand clusters at MIT Office of Research Computing and Data (ORCD) and MIT SuperCloud. This work additionally used Bridges-2 at Pittsburgh Supercomputing Center (PSC) through allocation MCH230021 from the Advanced Cyberinfrastructure Coordination Ecosystem: Services & Support (ACCESS) program, which is supported by National Science Foundation Grants No. 2138259, 2138286, 2138307, 2137603, and 2138296E.L. is supported by the National Science Foundation Graduate Research Fellowship under Grant No. 2141064.
文摘Machine learning(ML)surrogate models offer a promising route to accelerate material property prediction,bypassing costly atomistic simulations.Here,we introduce IsothermODE,a neural ordinary differential equation(NODE)framework for reconstructing full uptake and heat of adsorption(ΔH_(ads))isotherms for CO_(2)adsorption in metal-organic frameworks(MOFs)using only sparse pressure data.Unlike traditional ML models,IsothermODE leverages the intrinsic structure of differential equations to produce smooth,physically-consistent predictions that generalize across wide pressure ranges.We demonstrate high-fidelity interpolation and extrapolation,even with only five pressure points.To address the stochasticity inherent in Grand Canonical Monte Carlo(GCMC)simulations,we integrate uncertainty quantification,yielding tight bounds on predicted enthalpy curves.We further interpret the learned latent dynamics in terms of adsorption thermodynamics and textural properties,offering insight into structure-property relationships.Finally,we demonstrate IsothermODE’s long-range interpolation and extrapolation capabilities with sparse isotherm data(5 pressure points)and large incomplete intervals featuring missing data between 4–40(case 1)and 25–50(case 2)bars.IsothermODE provides a fast,robust alternative to simulation-heavy workflows,enabling scalable screening and design of next-generation carbon capture materials.
文摘探讨基于广义语言障碍临床思考框架建立的“语言-社交沟通-认知”三维度儿童语言评估与干预模式。首先,梳理神经发育性障碍儿童的常见语言与沟通问题,列举了符合三维度模式的循证早期语言干预体系。随着儿童进入学龄前期和学龄期,在鉴别诊断的过程中通过此模型分析出语言障碍、孤独症谱系障碍、注意缺陷多动障碍、智力障碍等不同根源问题。早期语言干预体系和单线的语言干预流程都不能有效推进儿童在学校学习和社交所需的关键技能。本文强调了三维模型中的“心智解读”(Theory of Mind)能力在这一阶段的重要作用,并专设章节进行详细阐述。最后,提出了将“语言-社交沟通-认知”三维度模式落实于实践的基于叙事的,三个维度协同推进的语言干预体系,并阐述了其循证基础和临床流程。
