Aeropropulsion System Test Facility (ASTF) is required to accurately control the pressure and temperature of the airflow to test the performance of the aero-engine. However, the control accuracy of ASTF is significant...Aeropropulsion System Test Facility (ASTF) is required to accurately control the pressure and temperature of the airflow to test the performance of the aero-engine. However, the control accuracy of ASTF is significantly affected by the flow disturbance caused by aero-engine acceleration and deceleration. This would reduce the credibility of ASTF’s test results for the aero-engine. Therefore, first, this paper proposes a feedforward compensation-based L1 adaptive control method for ASTF to address this problem. The baseline controller is first designed based on ideal uncoupled closed-loop dynamics to achieve dynamic decoupling. Then, L1 adaptive control is adopted to deal with various uncertainties and ensure good control performance. To further enhance the anti-disturbance performance, a feedforward strategy based on disturbance prediction is designed in the L1 adaptive control framework to compensate for the unmatched flow disturbance, which cannot be measured directly. In addition, this strategy takes into account the effects of actuator dynamics. With this method, the feedforward term can be determined from the nominal model parameters despite uncertainties. Finally, to demonstrate the effectiveness of the proposed method, various comparative experiments are performed on a hardware-in-the-loop system of ASTF. The experimental results show that the proposed method possesses excellent tracking performance, anti-disturbance performance and robustness.展开更多
In this paper, the attitude control algorithm of flexible spacecraft with unknown measurement delay and input delay based on disturbance observer is designed. The influence of measurement delay and input delay on the ...In this paper, the attitude control algorithm of flexible spacecraft with unknown measurement delay and input delay based on disturbance observer is designed. The influence of measurement delay and input delay on the attitude control system and disturbance observer is analyzed. The disturbance estimation error equation is transformed into a differential system with a pure delay. Then, the observer gain is chosen based on the 3/2 stability theorem to ensure the stability and disturbance attenuation performance of the pure delay system. Next, the controller gain is designed based on the Linear Matrix Inequality(LMI) approach to guarantee the stability of the composite system and achieve H_∞ performance with two additive delays. The simulation results show that the proposed method can improve the anti-disturbance ability of the attitude control system.展开更多
This article investigates gain self-scheduled H 1 robust control system design for a tailless fold- ing-wing morphing aircraft in the wing shape varying process. During the wing morphing phase, the aircraft＇s dynamic...This article investigates gain self-scheduled H 1 robust control system design for a tailless fold- ing-wing morphing aircraft in the wing shape varying process. During the wing morphing phase, the aircraft＇s dynamic response will be governed by time-varying aerodynamic forces and moments. Nonlinear dynamic equations of the morphing aircraft are linearized by using Jacobian linearization approach, and a linear parameter varying （LPV） model of the morphing aircraft in wing folding is obtained. A multi-loop controller for the morphing aircraft is formulated to guarantee stability for the wing shape transition process. The proposed controller uses a set of inner-loop gains to provide stability using classical techniques, whereas a gain self-scheduled H 1 outer-loop controller is devised to guarantee a specific level of robust stability and performance for the time-varying dynamics. The closed-loop simulations show that speed and altitude vary slightly during the whole wing folding process, and they converge rapidly after the process ends. This proves that the gain self-scheduled H 1 robust controller can guarantee a satisfactory dynamic performance for the morphing aircraft during the whole wing shape transition process. Finally, the flight control system＇s robustness for the wing folding process is verified according to uncertainties of the aerodynamic parameters in the nonlinear model.展开更多
This paper presents an adaptive control scheme with an integration of sliding mode control into the L1 adaptive control architecture, which provides good tracking performance as well as robustness against matched unce...This paper presents an adaptive control scheme with an integration of sliding mode control into the L1 adaptive control architecture, which provides good tracking performance as well as robustness against matched uncertainties. Sliding mode control is used as an adaptive law in the L1 adaptive control architecture, which is considered as a virtual control of error dynamics between estimated states and real states. Low-pass filtering mechanism in the control law design prevents a discontinuous signal in the adaptive law from appearing in actual control signal while maintaining control accuracy. By using sliding mode control as a virtual control of error dynamics and introducing the low-pass filtered control signal, the chattering effect is eliminated. The performance bounds between the close-loop adaptive system and the closed-loop reference system are characterized in this paper. Numerical simulation is provided to demonstrate the performance of the presented adaptive control scheme.展开更多
Rice is one of the most consumed staple food plants around the world, and its plant architecture is very important to improve the grain yield （Zhang et al., 2008）. Plant height, leaf angle, tiller number and angle, ...Rice is one of the most consumed staple food plants around the world, and its plant architecture is very important to improve the grain yield （Zhang et al., 2008）. Plant height, leaf angle, tiller number and angle, and uniformity of panicle layer all can have strong effects on grain yield （Wang and Li, 2008）. During the long history of domestication, rice has been selected to develop uniform tiller height architecture that ensures panicle layer uniformity and ease of harvesting （Ma et al., 2009）, and is largely determined by the synchronic culm elongation.展开更多
The main task of this work is to design a control system for a small tail-sitter Unmanned Aerial Vehicle(UAV)during the transition process.Although reasonable control performance can be obtained through a well-tuned s...The main task of this work is to design a control system for a small tail-sitter Unmanned Aerial Vehicle(UAV)during the transition process.Although reasonable control performance can be obtained through a well-tuned single PID or cascade PID control architecture under nominal conditions,large or fast time-varying disturbances and a wide range of changes in the equilibrium point bring nonlinear characteristics to the transition control during the transition process,which leads to control precision degradation.Meanwhile,the PID controller’s tuning method relies on engineering experiences to a certain extent and the controller parameters need to be retuned under different working conditions,which limits the rapid deployment and preliminary validation.Based on the above issues,a novel control architecture of L1 neural network adaptive control associated with PID control is proposed to improve the compensation ability during the transition process and guarantee the security transition.The L1 neural network adaptive control is revised to solve the multi-input and multi-output problem of the tail-sitter UAV system in this study.Finally,the transition characteristics of the time setting difference between the desired transition speed and the desired transition pitch angle are analyzed.展开更多
Objective: Our previous studies have firstly demonstrated that 17β -E2 up-regulates LRP16 gene expression in human breast cancer MCF-7 cells, and ectopic expression of the LRP16 gene promotes MCF-7 cells proliferatio...Objective: Our previous studies have firstly demonstrated that 17β -E2 up-regulates LRP16 gene expression in human breast cancer MCF-7 cells, and ectopic expression of the LRP16 gene promotes MCF-7 cells proliferation. Here, the effects of the LRP16 gene expression on growth of MCF-7 human breast cancer cells and the mechanism were further studied by establishing two stably LRP16-inhibitory MCR-7 cell lines. Methods: Hairpin small interference RNA (siRNA) strategy, by which hairpin siRNA was released by U6 promoter and was mediated by pLPC-based retroviral vector, was adopted to knockdown endogenous LRP16 level in MCF-7 cells. And the hairpin siRNA against green fluorescence protein (GFP) was used as the negative control. The suppressant efficiency of the LRP16 gene expression was confirmed by Nothern blot. Cell proliferation assay and soft agar colony formation assay were used to determine the status of the cells proliferation. Cell cycle checkpoints including cyclin E and cyclin D1 were examined by Western blot. Results: The results from cell proliferation assays suggested that down-regulation of LRP16 gene expression is capable of inhibiting MCF-7 breast cancer cell growth and down-regulation of the LRP16 gene expression is able to inhibit anchorage-independent growth of breast cancer cells in soft agar. We also demonstrated that cyclin E and cyclin D1 proteins were much lower in the LRP16-inhibitory cells than in the control cells. Conclusion: These data suggest that LRP16 gene play an important role in MCF-7 cells proliferation by regulating the pathway of the G1/S transition and may function as an important modulator in regulating the process of tumorigenesis in human breast.展开更多
Osteoarthritis(OA)is a prevalent joint disease with no effective treatment strategies.Aberrant mechanical stimuli was demonstrated to be an essential factor for OA pathogenesis.Although multiple studies have detected ...Osteoarthritis(OA)is a prevalent joint disease with no effective treatment strategies.Aberrant mechanical stimuli was demonstrated to be an essential factor for OA pathogenesis.Although multiple studies have detected potential regulatory mechanisms underlying OA and have concentrated on developing novel treatment strategies,the epigenetic control of OA remains unclear.Histone demethylase JMJD3 has been reported to mediate multiple physiological and pathological processes,including cell differentiation,proliferation,autophagy,and apoptosis.However,the regulation of JMJD3 in aberrant force-related OA and its mediatory effect on disease progression are still unknown.In this work,we confirmed the upregulation of JMJD3 in aberrant forceinduced cartilage injury in vitro and in vivo.Functionally,inhibition of JMJD3 by its inhibitor,GSK-J4,or downregulation of JMJD3 by adenovirus infection of sh-JMJD3 could alleviate the aberrant force-induced chondrocyte injury.Mechanistic investigation illustrated that aberrant force induces JMJD3 expression and then demethylates H3K27me3 at the NR4A1 promoter to promote its expression.Further experiments indicated that NR4A1 can regulate chondrocyte apoptosis,cartilage degeneration,extracellular matrix degradation,and inflammatory responses.In vivo,anterior cruciate ligament transection(ACLT)was performed to construct an OA model,and the therapeutic effect of GSK-J4 was validated.More importantly,we adopted a peptide-si RNA nanoplatform to deliver si-JMJD3 into articular cartilage,and the severity of joint degeneration was remarkably mitigated.Taken together,our findings demonstrated that JMJD3 is flow-responsive and epigenetically regulates OA progression.Our work provides evidences for JMJD3 inhibition as an innovative epigenetic therapy approach for joint diseases by utilizing p5RHH-si RNA nanocomplexes.展开更多
基金supported by the“Shuimu Tsinghua Scholar”Project,China(No.2024SM223)the National Science and Technology Major Project,China(No.Y2022-V-0002-0028).
文摘Aeropropulsion System Test Facility (ASTF) is required to accurately control the pressure and temperature of the airflow to test the performance of the aero-engine. However, the control accuracy of ASTF is significantly affected by the flow disturbance caused by aero-engine acceleration and deceleration. This would reduce the credibility of ASTF’s test results for the aero-engine. Therefore, first, this paper proposes a feedforward compensation-based L1 adaptive control method for ASTF to address this problem. The baseline controller is first designed based on ideal uncoupled closed-loop dynamics to achieve dynamic decoupling. Then, L1 adaptive control is adopted to deal with various uncertainties and ensure good control performance. To further enhance the anti-disturbance performance, a feedforward strategy based on disturbance prediction is designed in the L1 adaptive control framework to compensate for the unmatched flow disturbance, which cannot be measured directly. In addition, this strategy takes into account the effects of actuator dynamics. With this method, the feedforward term can be determined from the nominal model parameters despite uncertainties. Finally, to demonstrate the effectiveness of the proposed method, various comparative experiments are performed on a hardware-in-the-loop system of ASTF. The experimental results show that the proposed method possesses excellent tracking performance, anti-disturbance performance and robustness.
基金supported by the National Natural Science Foundation of China (Nos. 61627810, 61320106010, 61633003, 61661136007 and 61603021)the Program for Changjiang Scholars and Innovative Research Team, China (No. IRT_16R03)Innovative Research Team of National Natural Science Foundation of China (No. 61421063)
文摘In this paper, the attitude control algorithm of flexible spacecraft with unknown measurement delay and input delay based on disturbance observer is designed. The influence of measurement delay and input delay on the attitude control system and disturbance observer is analyzed. The disturbance estimation error equation is transformed into a differential system with a pure delay. Then, the observer gain is chosen based on the 3/2 stability theorem to ensure the stability and disturbance attenuation performance of the pure delay system. Next, the controller gain is designed based on the Linear Matrix Inequality(LMI) approach to guarantee the stability of the composite system and achieve H_∞ performance with two additive delays. The simulation results show that the proposed method can improve the anti-disturbance ability of the attitude control system.
基金co-supported by China Postdoctoral Science Foundation(Nos.20110490259,2012T50038)
文摘This article investigates gain self-scheduled H 1 robust control system design for a tailless fold- ing-wing morphing aircraft in the wing shape varying process. During the wing morphing phase, the aircraft＇s dynamic response will be governed by time-varying aerodynamic forces and moments. Nonlinear dynamic equations of the morphing aircraft are linearized by using Jacobian linearization approach, and a linear parameter varying （LPV） model of the morphing aircraft in wing folding is obtained. A multi-loop controller for the morphing aircraft is formulated to guarantee stability for the wing shape transition process. The proposed controller uses a set of inner-loop gains to provide stability using classical techniques, whereas a gain self-scheduled H 1 outer-loop controller is devised to guarantee a specific level of robust stability and performance for the time-varying dynamics. The closed-loop simulations show that speed and altitude vary slightly during the whole wing folding process, and they converge rapidly after the process ends. This proves that the gain self-scheduled H 1 robust controller can guarantee a satisfactory dynamic performance for the morphing aircraft during the whole wing shape transition process. Finally, the flight control system＇s robustness for the wing folding process is verified according to uncertainties of the aerodynamic parameters in the nonlinear model.
文摘This paper presents an adaptive control scheme with an integration of sliding mode control into the L1 adaptive control architecture, which provides good tracking performance as well as robustness against matched uncertainties. Sliding mode control is used as an adaptive law in the L1 adaptive control architecture, which is considered as a virtual control of error dynamics between estimated states and real states. Low-pass filtering mechanism in the control law design prevents a discontinuous signal in the adaptive law from appearing in actual control signal while maintaining control accuracy. By using sliding mode control as a virtual control of error dynamics and introducing the low-pass filtered control signal, the chattering effect is eliminated. The performance bounds between the close-loop adaptive system and the closed-loop reference system are characterized in this paper. Numerical simulation is provided to demonstrate the performance of the presented adaptive control scheme.
基金supported by funds from the National Transgenic Major Program Grants(No.2009ZX08009-022B)
文摘Rice is one of the most consumed staple food plants around the world, and its plant architecture is very important to improve the grain yield （Zhang et al., 2008）. Plant height, leaf angle, tiller number and angle, and uniformity of panicle layer all can have strong effects on grain yield （Wang and Li, 2008）. During the long history of domestication, rice has been selected to develop uniform tiller height architecture that ensures panicle layer uniformity and ease of harvesting （Ma et al., 2009）, and is largely determined by the synchronic culm elongation.
基金supported by the Natural Science Basic Research Plan in Shaanxi Province,China(No.2021JQ-214)the Fundamental Research Funds for the Central Universities,China(No.300102251101).
文摘The main task of this work is to design a control system for a small tail-sitter Unmanned Aerial Vehicle(UAV)during the transition process.Although reasonable control performance can be obtained through a well-tuned single PID or cascade PID control architecture under nominal conditions,large or fast time-varying disturbances and a wide range of changes in the equilibrium point bring nonlinear characteristics to the transition control during the transition process,which leads to control precision degradation.Meanwhile,the PID controller’s tuning method relies on engineering experiences to a certain extent and the controller parameters need to be retuned under different working conditions,which limits the rapid deployment and preliminary validation.Based on the above issues,a novel control architecture of L1 neural network adaptive control associated with PID control is proposed to improve the compensation ability during the transition process and guarantee the security transition.The L1 neural network adaptive control is revised to solve the multi-input and multi-output problem of the tail-sitter UAV system in this study.Finally,the transition characteristics of the time setting difference between the desired transition speed and the desired transition pitch angle are analyzed.
基金This work was supported by NationalNatural Science Foundation of China (No. 30200095).
文摘Objective: Our previous studies have firstly demonstrated that 17β -E2 up-regulates LRP16 gene expression in human breast cancer MCF-7 cells, and ectopic expression of the LRP16 gene promotes MCF-7 cells proliferation. Here, the effects of the LRP16 gene expression on growth of MCF-7 human breast cancer cells and the mechanism were further studied by establishing two stably LRP16-inhibitory MCR-7 cell lines. Methods: Hairpin small interference RNA (siRNA) strategy, by which hairpin siRNA was released by U6 promoter and was mediated by pLPC-based retroviral vector, was adopted to knockdown endogenous LRP16 level in MCF-7 cells. And the hairpin siRNA against green fluorescence protein (GFP) was used as the negative control. The suppressant efficiency of the LRP16 gene expression was confirmed by Nothern blot. Cell proliferation assay and soft agar colony formation assay were used to determine the status of the cells proliferation. Cell cycle checkpoints including cyclin E and cyclin D1 were examined by Western blot. Results: The results from cell proliferation assays suggested that down-regulation of LRP16 gene expression is capable of inhibiting MCF-7 breast cancer cell growth and down-regulation of the LRP16 gene expression is able to inhibit anchorage-independent growth of breast cancer cells in soft agar. We also demonstrated that cyclin E and cyclin D1 proteins were much lower in the LRP16-inhibitory cells than in the control cells. Conclusion: These data suggest that LRP16 gene play an important role in MCF-7 cells proliferation by regulating the pathway of the G1/S transition and may function as an important modulator in regulating the process of tumorigenesis in human breast.
基金supported by National Natural Science Foundation of China(11932012,81870790 and 31801233)Science and Technology Commission of Shanghai Municipality(18441903600)+1 种基金Clinical Research Plan of SHDC(No.SHDC2020CR3009A)Innovative Research Team of High-level Local Universities in Shanghai(SSMU-ZDCX20180902)。
文摘Osteoarthritis(OA)is a prevalent joint disease with no effective treatment strategies.Aberrant mechanical stimuli was demonstrated to be an essential factor for OA pathogenesis.Although multiple studies have detected potential regulatory mechanisms underlying OA and have concentrated on developing novel treatment strategies,the epigenetic control of OA remains unclear.Histone demethylase JMJD3 has been reported to mediate multiple physiological and pathological processes,including cell differentiation,proliferation,autophagy,and apoptosis.However,the regulation of JMJD3 in aberrant force-related OA and its mediatory effect on disease progression are still unknown.In this work,we confirmed the upregulation of JMJD3 in aberrant forceinduced cartilage injury in vitro and in vivo.Functionally,inhibition of JMJD3 by its inhibitor,GSK-J4,or downregulation of JMJD3 by adenovirus infection of sh-JMJD3 could alleviate the aberrant force-induced chondrocyte injury.Mechanistic investigation illustrated that aberrant force induces JMJD3 expression and then demethylates H3K27me3 at the NR4A1 promoter to promote its expression.Further experiments indicated that NR4A1 can regulate chondrocyte apoptosis,cartilage degeneration,extracellular matrix degradation,and inflammatory responses.In vivo,anterior cruciate ligament transection(ACLT)was performed to construct an OA model,and the therapeutic effect of GSK-J4 was validated.More importantly,we adopted a peptide-si RNA nanoplatform to deliver si-JMJD3 into articular cartilage,and the severity of joint degeneration was remarkably mitigated.Taken together,our findings demonstrated that JMJD3 is flow-responsive and epigenetically regulates OA progression.Our work provides evidences for JMJD3 inhibition as an innovative epigenetic therapy approach for joint diseases by utilizing p5RHH-si RNA nanocomplexes.
