目的本研究旨在探讨转录共激活因子(transcriptional coactivator with PDZ-binding motif,TAZ)与组蛋白H2A(histone H2A,H2A)的一种组蛋白变体H2A.Z,在缺氧诱导的肺损伤后修复中的作用及其潜在机制。方法利用缺氧模拟仓(5800 m)处理4 d...目的本研究旨在探讨转录共激活因子(transcriptional coactivator with PDZ-binding motif,TAZ)与组蛋白H2A(histone H2A,H2A)的一种组蛋白变体H2A.Z,在缺氧诱导的肺损伤后修复中的作用及其潜在机制。方法利用缺氧模拟仓(5800 m)处理4 d后,构建小鼠缺氧肺损伤模型,肺组织切片HE染色评估肺损伤程度。利用缺氧工作站(1%O_(2)浓度)处理小鼠肺泡上皮细胞系(murine lung epithelial-12,MLE12)24 h,构建MLE12缺氧细胞模型,Western blot分析检测TAZ表达。CCK-8实验评价肺泡上皮细胞(alveolar epithelial cells,AECs)增殖。免疫共沉淀(co-immunoprecipitation,Co-IP)实验验证TAZ与H2A.Z二者之间的相互作用。靶向剪切及转座酶标记技术(cleavage under targets and tagmentation,CUT&Tag)测序分析TAZ影响H2A.Z在染色质沉积情况。结果缺氧显著诱导小鼠肺组织肺泡萎缩及炎症浸润(P<0.01)。缺氧培养显著上调MLE12细胞中TAZ蛋白表达(P<0.05)。CCK-8实验发现,敲低TAZ后AECs增殖能力显著下降(P<0.01)。Co-IP证实TAZ与H2A.Z存在物理相互作用。靶向剪切及转座酶标记技术(cleavage under targets and tagmentation,CUT&Tag)测序显示缺氧促进H2A.Z在染色质上的沉积(常氧结合位点31817,缺氧结合位点44078),而TAZ敲低部分逆转此现象(结合位点37840)。结论缺氧显著上调TAZ表达,后者与H2A.Z的结合并促进H2A.Z在染色质上的沉积,增强AECs增殖以应对缺氧损伤。展开更多
Figure 3(d)in the paper[Chin.Phys.B 34057301(2025)]contained typos in the ZT values.Figure A4 contained typo in the label.The correct figures are provided.This modification does not affect the result presented in the ...Figure 3(d)in the paper[Chin.Phys.B 34057301(2025)]contained typos in the ZT values.Figure A4 contained typo in the label.The correct figures are provided.This modification does not affect the result presented in the paper.展开更多
It is of great importance to explore sustainable and eco-friendly recycling strategies for spent Li-ion batteries(LIBs).As such,the closed-loop resynthesis of LiNi_(x)CoyMn_(z)O_(2)(NCM)becomes recently popular as exe...It is of great importance to explore sustainable and eco-friendly recycling strategies for spent Li-ion batteries(LIBs).As such,the closed-loop resynthesis of LiNi_(x)CoyMn_(z)O_(2)(NCM)becomes recently popular as exemplified by the commercialization of low-and mid-Ni content NCM(33%-60%).However,there has been suspicion as to the successful deployment of Ni-rich NCM resynthesis process.Therefore,we systematically increase the Ni content of NCM from 60%to 90%from the industrial leachate of spent LIBs containing various metallic and nonmetallic impurities.The utilization rate of the leachate decreases from 71.8 mol%for NCM622 to 18.0 mol%for NCM955 as the Ni content in the NCM composition increases with the Co recycling rate being 100%in all resynthesized NCM(RNCM).The physicochemical and electrochemical properties of RNCM are systematically compared with its pristine NCM counterparts.As a result,various physicochemical properties of RNCM including impurity content,crystallographic information,morphology,particle size,porosity,specific surface area,elemental distribution,residual lithium compounds,and thermal stability are correlated with its electrochemical properties.It is found out that Al is the most critical impurity that determines the physicochemical and electrochemical properties of RNCM.It is noteworthy that RNCM955 prepared from spent LIBs without any purification step surpasses NCM955 in terms of rate and cycle performance.Further,this resynthesis approach toward Nirich NCM could meet the forthcoming 2031 EU's legislative target on the mandatory minimum recycling usage of valuable metals from spent LIBs.The anode active material was resynthesized using industrial leachate as the maximum.The amount of leachate used and the amount of impurities were proportional.展开更多
文摘目的本研究旨在探讨转录共激活因子(transcriptional coactivator with PDZ-binding motif,TAZ)与组蛋白H2A(histone H2A,H2A)的一种组蛋白变体H2A.Z,在缺氧诱导的肺损伤后修复中的作用及其潜在机制。方法利用缺氧模拟仓(5800 m)处理4 d后,构建小鼠缺氧肺损伤模型,肺组织切片HE染色评估肺损伤程度。利用缺氧工作站(1%O_(2)浓度)处理小鼠肺泡上皮细胞系(murine lung epithelial-12,MLE12)24 h,构建MLE12缺氧细胞模型,Western blot分析检测TAZ表达。CCK-8实验评价肺泡上皮细胞(alveolar epithelial cells,AECs)增殖。免疫共沉淀(co-immunoprecipitation,Co-IP)实验验证TAZ与H2A.Z二者之间的相互作用。靶向剪切及转座酶标记技术(cleavage under targets and tagmentation,CUT&Tag)测序分析TAZ影响H2A.Z在染色质沉积情况。结果缺氧显著诱导小鼠肺组织肺泡萎缩及炎症浸润(P<0.01)。缺氧培养显著上调MLE12细胞中TAZ蛋白表达(P<0.05)。CCK-8实验发现,敲低TAZ后AECs增殖能力显著下降(P<0.01)。Co-IP证实TAZ与H2A.Z存在物理相互作用。靶向剪切及转座酶标记技术(cleavage under targets and tagmentation,CUT&Tag)测序显示缺氧促进H2A.Z在染色质上的沉积(常氧结合位点31817,缺氧结合位点44078),而TAZ敲低部分逆转此现象(结合位点37840)。结论缺氧显著上调TAZ表达,后者与H2A.Z的结合并促进H2A.Z在染色质上的沉积,增强AECs增殖以应对缺氧损伤。
文摘Figure 3(d)in the paper[Chin.Phys.B 34057301(2025)]contained typos in the ZT values.Figure A4 contained typo in the label.The correct figures are provided.This modification does not affect the result presented in the paper.
基金supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science and ICT(RS-2023-00254424)by the ITRC(Information Technology Research Center)support program(IITP-2024-RS-2024-00437494)supervised by the IITP(Institute for Information&Communications Technology Planning&Evaluation)funded by the MSIT(Ministry of Science and ICT),Korea。
文摘It is of great importance to explore sustainable and eco-friendly recycling strategies for spent Li-ion batteries(LIBs).As such,the closed-loop resynthesis of LiNi_(x)CoyMn_(z)O_(2)(NCM)becomes recently popular as exemplified by the commercialization of low-and mid-Ni content NCM(33%-60%).However,there has been suspicion as to the successful deployment of Ni-rich NCM resynthesis process.Therefore,we systematically increase the Ni content of NCM from 60%to 90%from the industrial leachate of spent LIBs containing various metallic and nonmetallic impurities.The utilization rate of the leachate decreases from 71.8 mol%for NCM622 to 18.0 mol%for NCM955 as the Ni content in the NCM composition increases with the Co recycling rate being 100%in all resynthesized NCM(RNCM).The physicochemical and electrochemical properties of RNCM are systematically compared with its pristine NCM counterparts.As a result,various physicochemical properties of RNCM including impurity content,crystallographic information,morphology,particle size,porosity,specific surface area,elemental distribution,residual lithium compounds,and thermal stability are correlated with its electrochemical properties.It is found out that Al is the most critical impurity that determines the physicochemical and electrochemical properties of RNCM.It is noteworthy that RNCM955 prepared from spent LIBs without any purification step surpasses NCM955 in terms of rate and cycle performance.Further,this resynthesis approach toward Nirich NCM could meet the forthcoming 2031 EU's legislative target on the mandatory minimum recycling usage of valuable metals from spent LIBs.The anode active material was resynthesized using industrial leachate as the maximum.The amount of leachate used and the amount of impurities were proportional.
