IP over DVB-S是基于卫星实现IP数据广播的关键技术,在远程教育等领域广泛应用.ULE封装协议相比传统MPE协议更加简练、高效,对IP网络支持更好.设计了IP over DVB-S接收系统,并对其在Linux中实现时的关键技术予以介绍.该系统具有兼容ULE/...IP over DVB-S是基于卫星实现IP数据广播的关键技术,在远程教育等领域广泛应用.ULE封装协议相比传统MPE协议更加简练、高效,对IP网络支持更好.设计了IP over DVB-S接收系统,并对其在Linux中实现时的关键技术予以介绍.该系统具有兼容ULE/MPE两种封装方式、支持IPv4/IPv6双协议的特点,在实际环境中进行了部署.展开更多
Monkey B virus(Macacine herpesvirus 1; BV) is noted for its extreme neurovirulence in humans. Since the vhs protein encoded by the UL41 gene has been shown to be a neurovirulence factor in the related human herpes sim...Monkey B virus(Macacine herpesvirus 1; BV) is noted for its extreme neurovirulence in humans. Since the vhs protein encoded by the UL41 gene has been shown to be a neurovirulence factor in the related human herpes simplex viruses, the role of the UL41 gene in BV neurovirulence was investigated. BV mutants were constructed that lacked the entire UL41 ORF(Δ41) or had the RNase active site mutated(Δ41A). Neither mutant shut off host protein synthesis, degraded β-actin mRNA, or prevented an IFN-β response, indicating that the vhs protein and its RNase activity are both necessary for these activities. Replication of both mutants in primary mouse cells was impaired and they exhibited a prolonged disease course in mice. Whereas Δ41 infected mice were euthanized for symptoms related to central nervous system(CNS) infection, Δ41A infected mice were euthanized primarily for symptoms of autonomic nervous system dysfunction. While neuroinvasiveness was not affected, lesions in the CNS were more limited in size, anatomical distribution, and severity than for wild-type virus. These results indicate that the vhs protein affects the general replicative efficiency of BV in vivo rather than being a specific neurovirulence factor critical for invasion of or preferential replication in the CNS.展开更多
An interaction between the HSV-1 UL25 capsid protein and cellular microtubule-associated protein was found using a yeast two-hybrid screen and β-D-galactosidase activity assays. Immunofluorescence microscopy of the U...An interaction between the HSV-1 UL25 capsid protein and cellular microtubule-associated protein was found using a yeast two-hybrid screen and β-D-galactosidase activity assays. Immunofluorescence microscopy of the UL25 protein demonstrated its co-localization with cellular microtubule-associated protein in the plasma membrane. Further investigations with deletion mutants suggest that UL25 is likely to have a function in the nucleus.展开更多
伪狂犬病病毒(PRV)是一种对猪和其他哺乳动物造成严重危害的病原体,它能够引发宿主的系统性炎症反应和炎症损伤。尽管已知PRV感染与炎性小体的激活和焦亡有关,但PRV促进炎性小体激活的具体分子机制尚不清楚。2024年9月24日,西北农林科...伪狂犬病病毒(PRV)是一种对猪和其他哺乳动物造成严重危害的病原体,它能够引发宿主的系统性炎症反应和炎症损伤。尽管已知PRV感染与炎性小体的激活和焦亡有关,但PRV促进炎性小体激活的具体分子机制尚不清楚。2024年9月24日,西北农林科技大学研究团队在PLoS Pathogens在线发表了题为“Pseudorabies virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation”的最新研究成果,鉴定出PRV非结构蛋白UL4的关键功能,揭示其如何增强ASC依赖性的炎性小体激活,促进焦亡,并加剧炎症反应。这一研究成果对于开发新的疫苗和治疗策略具有重要意义。展开更多
文摘IP over DVB-S是基于卫星实现IP数据广播的关键技术,在远程教育等领域广泛应用.ULE封装协议相比传统MPE协议更加简练、高效,对IP网络支持更好.设计了IP over DVB-S接收系统,并对其在Linux中实现时的关键技术予以介绍.该系统具有兼容ULE/MPE两种封装方式、支持IPv4/IPv6双协议的特点,在实际环境中进行了部署.
基金supported in part by PHS grants 2P40 OD010988 and 1P40 OD010431
文摘Monkey B virus(Macacine herpesvirus 1; BV) is noted for its extreme neurovirulence in humans. Since the vhs protein encoded by the UL41 gene has been shown to be a neurovirulence factor in the related human herpes simplex viruses, the role of the UL41 gene in BV neurovirulence was investigated. BV mutants were constructed that lacked the entire UL41 ORF(Δ41) or had the RNase active site mutated(Δ41A). Neither mutant shut off host protein synthesis, degraded β-actin mRNA, or prevented an IFN-β response, indicating that the vhs protein and its RNase activity are both necessary for these activities. Replication of both mutants in primary mouse cells was impaired and they exhibited a prolonged disease course in mice. Whereas Δ41 infected mice were euthanized for symptoms related to central nervous system(CNS) infection, Δ41A infected mice were euthanized primarily for symptoms of autonomic nervous system dysfunction. While neuroinvasiveness was not affected, lesions in the CNS were more limited in size, anatomical distribution, and severity than for wild-type virus. These results indicate that the vhs protein affects the general replicative efficiency of BV in vivo rather than being a specific neurovirulence factor critical for invasion of or preferential replication in the CNS.
基金National Natural Science Funds(30570081, 30670094 and 30700028)
文摘An interaction between the HSV-1 UL25 capsid protein and cellular microtubule-associated protein was found using a yeast two-hybrid screen and β-D-galactosidase activity assays. Immunofluorescence microscopy of the UL25 protein demonstrated its co-localization with cellular microtubule-associated protein in the plasma membrane. Further investigations with deletion mutants suggest that UL25 is likely to have a function in the nucleus.
文摘伪狂犬病病毒(PRV)是一种对猪和其他哺乳动物造成严重危害的病原体,它能够引发宿主的系统性炎症反应和炎症损伤。尽管已知PRV感染与炎性小体的激活和焦亡有关,但PRV促进炎性小体激活的具体分子机制尚不清楚。2024年9月24日,西北农林科技大学研究团队在PLoS Pathogens在线发表了题为“Pseudorabies virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation”的最新研究成果,鉴定出PRV非结构蛋白UL4的关键功能,揭示其如何增强ASC依赖性的炎性小体激活,促进焦亡,并加剧炎症反应。这一研究成果对于开发新的疫苗和治疗策略具有重要意义。
