摘要
鸭瘟是一种由鸭瘟病毒引起的急性、热性、高度接触性传染病,对养鸭业造成了严重威胁。目前,该病主要依靠鸭瘟弱毒活疫苗进行防控。鸭瘟病毒属于疱疹病毒科,其基因组为双链DNA,具有复杂的结构和多种变异类型。为建立一种能够区分鸭瘟病毒强毒株与弱毒株的双重PCR鉴别检测方法,以提高临床诊断的准确性和效率,通过分析鸭瘟病毒基因组序列,设计针对UL2和LORF5基因的特异性引物,建立了双重PCR方法。该方法具有良好的特异性,能够准确区分鸭瘟病毒强毒株与弱毒株,同时对其他禽类病原无交叉反应:最低可检测到10拷贝的鸭瘟病毒DNA,表现出较高的灵敏度。使用建立的方法对2019-2024年收集的临床疑似鸭瘟病料进行检测,发现经典强毒株集中流行于2019-2022年,而变异强毒株在2023-2024年更为常见,表明鸭瘟病毒优势流行株可能正在发生变化。综上,本研究建立的双重PCR鉴别检测方法为鸭瘟病毒的快速、准确检测提供了技术支持,对鸭瘟防控及流行病学调查具有重要意义。
Duck plague(DP)is an acute,febrile and highly contagious disease caused by duck plague virus(DPV),posing a serious threat to duck industry.Currently,it is mainly prevented and controlled by live attenuated DP vaccine.DPV,a member of the family Herpesviridae,possesses a double-stranded DNA(dsDNA)genome characterized by complex structure and a variety of variants.In order to distinguish virulent and attenuated DPV,and to improve accuracy and efficiency of clinical diagnosis,a dual PCR assay was established based on specific primers designed for the genes of UL2 and LORF5 through analyzing the genome sequence of the virus.The method could be used to accurately identify the virulent and attenuated DPV,without cross-reaction to other avian pathogens,indicating a good specificity:and its detection limit was as low as 10 copies of DPV DNA,demonstrating a high sensitivity.Clinical samples suspected to be infected with DPV collected from 2019 to 2024 were detected by the established method,it was found that classical virulent strains predominated from 2019 to 2022,while the variant ones were more prevalent from 2023 to 2024,indicating a potential shift in the dominant strains.In conclusion,the dua1 PCR assay established in the study could be used to rapidly and accurately detect DPV as a technical tool,contributing to control strategies and epidemiological investigation against the disease.
作者
周婉婷
崔雪志
毛秋艳
汪建华
李玉峰
李金平
刘朔
彭程
杨吉喆
侯广宇
刘华雷
蒋文明
Zhou Wanting;Cui Xuezhi;Mao Qiuyan;Wang Jianhua;Li Yufeng;Li Jinping;Liu Shuo;Peng Cheng;Yang Jizhe;Hou Guangyu;Liu Hualei;Jiang Wenming(China Animal Health and Epidemiology Center,Qingdao 266032,Shandong,China;Shandong Zhongxin Food Group Co.,Ltd.,Jinan 250102,Shandong,China;Shandong Hekangyuan Biological Breeding Co.,Ltd.,Jinan 250100,Shandong,China;Poultry Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250023,Shandong,China)
出处
《中国动物检疫》
2025年第8期108-114,共7页
China Animal Health Inspection
基金
山东省重点研发计划项目(2022CXGC010606)。
