In order to further study functions of the porcine myostatin gene, we analyzed the polymorphisms of porcine myostatin gene in promoter region among different breeds including Yorkshire, Landrace, Duroc, Junmu, Min pig...In order to further study functions of the porcine myostatin gene, we analyzed the polymorphisms of porcine myostatin gene in promoter region among different breeds including Yorkshire, Landrace, Duroc, Junmu, Min pig and Sanjiang white pig by PCR-RFLPs. The allele T dominated in the imported lean-type pig breeds such as Yorkshire, Landrace and Duroc. No allele A was detected in Junmu and Sanjiang white pig, and the frequencies of three genotypes were about equal in Min pig. The result using X2 analysis showed that the distribution of three genotypes was related to pig breeds.展开更多
Schistosomiasis is a public health concern in Benin. Freshwater snails of the genus Bulinus serve as intermediate hosts for schistosomes, trematode parasites responsible for bilharzia. The urinary form, caused by Schi...Schistosomiasis is a public health concern in Benin. Freshwater snails of the genus Bulinus serve as intermediate hosts for schistosomes, trematode parasites responsible for bilharzia. The urinary form, caused by Schistosoma haematobium, is the most widespread and is transmitted to humans by these mollusks, with Bulinus truncatus and Bulinus globosus being the most important species. Effective strategies to combat the transmission of these parasites require a prior understanding of the molecular characterization of Bulinus snails. For this purpose, 293 Bulinus snails were collected and morphologically identified from two localities in southern Benin, Sô-Ava and Azowlissè. The snails were preserved in absolute alcohol. To achieve the set objectives, DNA was extracted from the collected biological material, and SSU gene fragments were amplified. Using PCR-RFLP, the amplified fragments were digested with the restriction endonucleases HaeIII, HinfI, and DdeI to perform molecular characterization. In this study, 80 individuals of B. globosus and 10 of B. truncatus were subjected to molecular analysis. The PCR-RFLP profiles showed bands of different sizes for the Bulinus species when analyzed with the three endonucleases using the SSU molecular marker. PCR-RFLP analysis revealed that the snails belonged to the freshwater genus Bulinus, including Bulinus globosus and B. truncatus, based on reference profiles from studies conducted in Nigeria, which enabled precise identification of these gastropods. This study provided initial insights, although still incomplete, into the molecular diversity of these species.展开更多
基金Key Items of Plan of Science and Technology of Heilongjiang Province (CGB01B104)
文摘In order to further study functions of the porcine myostatin gene, we analyzed the polymorphisms of porcine myostatin gene in promoter region among different breeds including Yorkshire, Landrace, Duroc, Junmu, Min pig and Sanjiang white pig by PCR-RFLPs. The allele T dominated in the imported lean-type pig breeds such as Yorkshire, Landrace and Duroc. No allele A was detected in Junmu and Sanjiang white pig, and the frequencies of three genotypes were about equal in Min pig. The result using X2 analysis showed that the distribution of three genotypes was related to pig breeds.
文摘Schistosomiasis is a public health concern in Benin. Freshwater snails of the genus Bulinus serve as intermediate hosts for schistosomes, trematode parasites responsible for bilharzia. The urinary form, caused by Schistosoma haematobium, is the most widespread and is transmitted to humans by these mollusks, with Bulinus truncatus and Bulinus globosus being the most important species. Effective strategies to combat the transmission of these parasites require a prior understanding of the molecular characterization of Bulinus snails. For this purpose, 293 Bulinus snails were collected and morphologically identified from two localities in southern Benin, Sô-Ava and Azowlissè. The snails were preserved in absolute alcohol. To achieve the set objectives, DNA was extracted from the collected biological material, and SSU gene fragments were amplified. Using PCR-RFLP, the amplified fragments were digested with the restriction endonucleases HaeIII, HinfI, and DdeI to perform molecular characterization. In this study, 80 individuals of B. globosus and 10 of B. truncatus were subjected to molecular analysis. The PCR-RFLP profiles showed bands of different sizes for the Bulinus species when analyzed with the three endonucleases using the SSU molecular marker. PCR-RFLP analysis revealed that the snails belonged to the freshwater genus Bulinus, including Bulinus globosus and B. truncatus, based on reference profiles from studies conducted in Nigeria, which enabled precise identification of these gastropods. This study provided initial insights, although still incomplete, into the molecular diversity of these species.
