[ Objective] This study aimed to screen and preliminarily identify LactobaciUus strain CW3. [ Method] Oxford cup double-plate method was used for preliminary screening of broad-spectrum bacteriacin-producing Lactobaci...[ Objective] This study aimed to screen and preliminarily identify LactobaciUus strain CW3. [ Method] Oxford cup double-plate method was used for preliminary screening of broad-spectrum bacteriacin-producing LactobaciUus strain; after eliminating the interference of organic acid and hydrogen peroxide, antibac- terial substances containing protein properties produced by the screened strain were detected; finally, the obtained strain was identified. [ Result] After elimination of interference factors such as organic acid and hydrogen peroxide, the fermentation supernatant of strain CW3 could stil｜ inhibit the growth of indicator strains; the antibacterial activity of fermentation broth was significantly reduced after treatment with trypsase and pepsase, which indicated that the produced antibacterial sub- stances were bacteriocins. Results of physiological and biochemical identification showed that strain CW3 belonged to Lactobacillus plantarum. [ Conclusion] CW3 is a broad-spectrum bacteriocin-producing LactobaciUus plantarum strain.展开更多
基金Supported by National Natural Science Foundation of China(41101244)Project of Basic and Advanced Research of Henan Province(122300410161)Natural Science Research Project of the Education Department of Henan Province(2010B180029)
文摘[ Objective] This study aimed to screen and preliminarily identify LactobaciUus strain CW3. [ Method] Oxford cup double-plate method was used for preliminary screening of broad-spectrum bacteriacin-producing LactobaciUus strain; after eliminating the interference of organic acid and hydrogen peroxide, antibac- terial substances containing protein properties produced by the screened strain were detected; finally, the obtained strain was identified. [ Result] After elimination of interference factors such as organic acid and hydrogen peroxide, the fermentation supernatant of strain CW3 could stil｜ inhibit the growth of indicator strains; the antibacterial activity of fermentation broth was significantly reduced after treatment with trypsase and pepsase, which indicated that the produced antibacterial sub- stances were bacteriocins. Results of physiological and biochemical identification showed that strain CW3 belonged to Lactobacillus plantarum. [ Conclusion] CW3 is a broad-spectrum bacteriocin-producing LactobaciUus plantarum strain.
