The microscopic investigation of the floral development of sweet cherry(Prunus avium L.cv.Hongdeng)from a warm winter climate(Shanghai)and cold winter climate(Tai'an,Shandong Province,China)was conducted to explor...The microscopic investigation of the floral development of sweet cherry(Prunus avium L.cv.Hongdeng)from a warm winter climate(Shanghai)and cold winter climate(Tai'an,Shandong Province,China)was conducted to explore the reason of low fruit set.The effect of hydrogen cyanamide(HCN)on floral development under warm winter conditions was also investigated.Trees grown in Shanghai with insufficient chilling accumulation exhibited little difference in the progression of microspore development compared to trees in Tai'an that accumulated adequate chilling,but showed substantial delays in ovule and embryo sac development.The growth of nucelli did not proceed beyond the macrospore mother cell and macrospore stages with abortion rates of 13,15 and 45%by 6,3 and 0 d before full bloom,respectively.These abnormalities in the ovule and embryo sac in the Shanghai-grown trees were eliminated by HCN application.These results suggest that chilling regulates the development of female floral organs in winter dormancy;therefore,insufficient chilling accumulation,causing abnormality of the female floral organs,restricts the cultivation of sweet cherry in warm winter regions.Interestingly,HCN application,which decreased the chilling requirements for Hongdeng,may be a potential strategy for sweet cherry cultivation in warm winter regions.展开更多
Transcription factor IIIA(TFIIIA)is a zinc finger protein that facilitates the assembly of a transcription complex by recruiting transcription factors TFIIIB and TFIIIC,along with RNA polymerase III,to initiate the tr...Transcription factor IIIA(TFIIIA)is a zinc finger protein that facilitates the assembly of a transcription complex by recruiting transcription factors TFIIIB and TFIIIC,along with RNA polymerase III,to initiate the transcription of 5S rRNA genes.However,the effects of TFIIIA knockout in vertebrates remain unclear.To address this,we investigated the function of a homologous general transcription factor IIIAa,gtf3aa,identified as a maternal factor in zebrafish.During early embryonic development,gtf3aa expression initially increased and subsequently declined.At 12 h postfertilization,gtf3aa mRNA was detected at notably low levels in the embryo,whereas by three days postfertilization,its mRNA level gradually increased in the larvae.The gtf3aa was broadly expressed in various embryonic tissues of zebrafish,with higher expression levels observed in the brain,heart,liver,and muscle.Knockout of gtf3aa significantly suppressed somatic 5S rRNA transcription in early zebrafish embryos and larvae,resulting in a reduction in the number of mature monoribosomes and polyribosomes.The gtf3aa−/−larvae exhibited slow growth and delayed yolk absorption,along with impaired development of the eyes,heart,swim bladder,liver,and intestinal tissues.Additionally,expression of genes involved in metabolic signaling pathways,including the peroxisome proliferator-activated receptor pathway,was reduced.The gtf3aa−/−zebrafish did not survive beyond seven days postfertilization.This study offers preliminary insights into the role of gtf3aa in regulating somatic 5S rRNA transcription and embryonic organ development in zebrafish.展开更多
The transition from vegetative to reproductive growth is a vital step for the reproductive success of plants.In Arabidopsis thaliana,LEAFY(LFY)plays crucial roles in inflorescence primordium and floral organ developme...The transition from vegetative to reproductive growth is a vital step for the reproductive success of plants.In Arabidopsis thaliana,LEAFY(LFY)plays crucial roles in inflorescence primordium and floral organ development,but little is known about the roles of its homologs in crop plants such as soybean(Glycine max).Here,we investigated the expression patterns and functions of the two LFY genes(LFY1 and LFY2)in soybean.Both genes were predominantly expressed in unopened flowers and the shoot apical meristem,with LFY2 having the higher transcript abundance.In an in situ hybridization assay,LFY genes produced strong signals in the floral meristem.We next generated lfy1 and lfy2 knockout lines.The lfy2 mutants showed obvious changes in floral organ morphology,but the lfy1 mutants showed no obvious changes in floral organ morphology or pod development.The lfy1 lfy2 double mutants displayed more serious defects in floral organ development than lfy2,resulting in complete sterility.Gene expression analysis revealed differences in expression of the A-class APETALA(AP)genes AP1a and AP1b in the double mutant lines.These results suggest that LFY2 plays an important role in floral organ formation in soybean by regulating the expression of homeotic genes.Our findings increase the understanding of floral development,which could be useful for flower designs during hybrid soybean breeding.展开更多
Taking the Lower Silurian Longmaxi Formation shale in the Sichuan Basin as an example,this study employs atomic force microscopy-based infrared(AFM-IR)spectroscopy to analyze the submicron-scale molecular functional g...Taking the Lower Silurian Longmaxi Formation shale in the Sichuan Basin as an example,this study employs atomic force microscopy-based infrared(AFM-IR)spectroscopy to analyze the submicron-scale molecular functional groups of different types and occurrences of organic matter.Combined with the quantitative evaluation of pore development via scanning electron microscopy(SEM),the response of organic pore formation and evolution mechanisms to chemical composition and structural evolution of organic matter in overmature marine shale is investigated.The results indicate that the AFM-IR spectra of graptolite periderms and pyrobitumen in shale are dominated by the stretching vibrations of conjugated C=C bonds in aromatic compounds at approximately 1600 cm-1,with weak absorption peaks near 1375,1450 and 1720 cm-1,corresponding to aliphatic chains and carbonyl/carboxyl functional groups.Overall,the AFM-IR structural indices(A and C factors)of organic matter show a strong correlation with visible porosity in shales of equivalent maturity.Lower A and C factor values correlate with enhanced development of organic pores,which is associated with the detachment of more aliphatic chains and oxygen-containing functional groups during thermal evolution.Pyrobitumen-clay mineral composites generally exhibit superior pore development,likely attributable to clay mineral dehydration participating in hydrocarbon generation reactions that promote the removal of more functional groups.Additionally,hydrocarbon generation within organic-clay composites during high-over mature stages may induce volumetric expansion,resulting in microfracturing and hydrocarbon expulsion.The associated higher hydrocarbon expulsion rates promote the formation of larger pores and fracture-shaped pores along the flake-shaped clay minerals.This study highlights that the research of submicron-scale molecular functional groups provides a deeper understanding of organic matter evolution and pores development mechanisms in overmature shales,thereby offering critical theoretical parameters for reservoir evaluation in shale oil and gas exploration.展开更多
This research extends the literature on the environmental Phillips curve(EPC)and environmental Kuznets curve(EKC)by focusing on the 38 member economies of the Organization for Economic Co-operation and Development(OEC...This research extends the literature on the environmental Phillips curve(EPC)and environmental Kuznets curve(EKC)by focusing on the 38 member economies of the Organization for Economic Co-operation and Development(OECD).Using panel data from 2000 to 2021,the study employs several econometric techniques,including fixed effects,feasible generalized least squares,two-stage least squares,and the generalized method of moments.Our primary findings reveal that unemployment has a significant negative impact on CO_(2)emissions,thereby supporting the validity of the EPC hypothesis within OECD countries.This suggests a trade-off between unemployment and reductions in CO_(2)emissions.Similarly,the results validate the EKC hypothesis,with further analysis indicating that the EKC exhibits an N-shaped curve-an important contribution to the literature on environmental dynamics in advanced economies.Additionally,the results show that both trade openness and renewable energy usage have significantly improved environmental quality in OECD economies.Finally,extensive causality testing identifies both one-way and two-way causal relationships among the key variables examined.These findings have important policy implications for the management of environmental quality and macroeconomic variables in the OECD context.展开更多
Mangrove degradation must reduce carbon sequestration in recent years, thereby aggravating global warming.Thus, short-term impacts of human activity on mangrove ecosystems are cause for concern from local governments ...Mangrove degradation must reduce carbon sequestration in recent years, thereby aggravating global warming.Thus, short-term impacts of human activity on mangrove ecosystems are cause for concern from local governments and scientists. Mangroves sediments can provide detailed records of mangrove species variation in the last one hundred years, based on detailed 210 Pb data. The study traced the history of mangrove development and its response to environmental change over the last 140 years in two mangrove swamps of Guangxi, Southwest China. Average sedimentation rates were calculated to be 0.48 cm/a and 0.56 cm/a in the Yingluo Bay and the Maowei Sea, respectively. Chemical indicators（δ13Corg and C：N） were utilized to trace the contribution of mangrove-derived organic matter（MOM） using a ternary mixing model. Simultaneous use of mangrove pollen can help to supplement some of these limitations in diagenetic/overlap of isotopic signatures. We found that vertical distribution of MOM was consistent with mangrove pollen, which could provide similar information for tracing mangrove ecosystems. Therefore, mangrove development was reconstructed and divided into three stages： flourishing, degradation and re-flourishing/re-degradation period. The significant degradation, found in the period of 1968–1998 and 1907–2007 in the Yingluo Bay and the Maowei Sea, respectively, corresponding to a rapid increase of reclamation area and seawall length, rather than climate change as recorded in the region.展开更多
Dear Editor： I would like to congratulate Zhou et al.[1] on their study of the correlation between expression of lysyl oxidase-like 1 （LOX-1） and fibulin-5 （F5） in the car- dinal ligament tissue and pelvic organ ...Dear Editor： I would like to congratulate Zhou et al.[1] on their study of the correlation between expression of lysyl oxidase-like 1 （LOX-1） and fibulin-5 （F5） in the car- dinal ligament tissue and pelvic organ prolapse （POP）. In their elegant work, they evaluated the levels of LOX-1 and F5 in connective tissue of the cardinal ligament in order to demonstrate signs of elastinopa- thy in women with POP. They stress the concept that several environmental risk factors could cause qualitative and quantitative changes in the connective tissue promoting POP. The above authors conclude that the specific mechanism of LOXL1 and F5 involved in the development of POP is unclear.展开更多
The study elaborates and examines a method for evaluation of organic farming development according to 10 indicators estimated by experts＇ opinion. It could be used for making comparisons in different countries, regio...The study elaborates and examines a method for evaluation of organic farming development according to 10 indicators estimated by experts＇ opinion. It could be used for making comparisons in different countries, regions, or even time periods on the basis of the 10 main indicators determined considering organic operators, organic area, organic production, organic farms＇ structure, resources, access to market, institutional support, education and training, science and technology, environmental protection, each one of them including sub-indicators. The proposed organic development index （ODI） could be used as a complex indicator for organic sector development embracing different and very significant aspects rather than only nowadays used organic area, percentage, sales, etc collected data for which are still questionable and difficult. The method was put into practice for six Balkan countries （Bulgaria, Serbia, Montenegro, Bosnia and Herzegovina, Turkey, and Romania）. The calculations of ODI show very low results--not well developed and competitive sector suffering the interference of international open markets. Turkey, Bulgaria, and Romania perform better while Western Balkan countries （Serbia, Montenegro, and Bosnia and Herzegovina） are still at the beginning of organic sector development.展开更多
The Maanshan Mining Research Institute of theMinistry of Metallurgical Industry is a composite miningscientific research institution,one of the units with rightsof conferring master degree approved by the State Counci...The Maanshan Mining Research Institute of theMinistry of Metallurgical Industry is a composite miningscientific research institution,one of the units with rightsof conferring master degree approved by the State Council,and enjoys foreign autonomous rights for import andexport,It has a staff of 1000,including 500 senior andintermediate technical people(42 professor-rank seniorengineers,and 39 enjoying government special subsidy). The institute has faculties including展开更多
The Administrative Board of theLianyungang Development Zone isan executive administrative organimplementing unified leadership andmanagement over the development zone onbehalf of the Lianyungang People’sgovernment. A...The Administrative Board of theLianyungang Development Zone isan executive administrative organimplementing unified leadership andmanagement over the development zone onbehalf of the Lianyungang People’sgovernment. According to the State’s lawsand regulations, the administrative board isresponsible for construction planning for thedevelopment zone, the execution of economicdevelopment programmes, the approval ofinvestment projects worth RMB30 millionor less, land use and building design, boostingpublic welfare in education, culture andsanitation, public security in the zone, andprotecting the legal rights of the展开更多
Objective To study how to promote the smooth innovation and transformation of enterprises and enhance the market competitiveness of enterprises from the perspective of human resources organization development.In the w...Objective To study how to promote the smooth innovation and transformation of enterprises and enhance the market competitiveness of enterprises from the perspective of human resources organization development.In the wave of pharmaceutical industry reform,some entrepreneurs realize the importance of innovation and technology in the future.However,domestic pharmaceutical enterprises are still in the production-oriented stage.In the process of transformative innovation,pharmaceutical enterprises should not only rely on the support of scientific and technological and economic forces,but also need a suitable organizational change and many excellent talents as the source of development.Methods Through retrieving relevant literature and combined with the organizational development practice of X pharmaceutical enterprise,the organizational development model was explored in this paper.Results and Conclusion By forming a system of strategy,organization,talent,culture and mechanism,and building an organizational development model,we can improve the organizational atmosphere and organizational efficiency in the process of enterprise innovation and transformation,so as to enhance the competitiveness of enterprises in the market.展开更多
Multiple ecological and socioeconomic problems have occurred worldwide,raising the awareness of sustainability.This study aims to examine the impact of taxes on Sustainable Development Goals(SDGs)in the context of Org...Multiple ecological and socioeconomic problems have occurred worldwide,raising the awareness of sustainability.This study aims to examine the impact of taxes on Sustainable Development Goals(SDGs)in the context of Organization for Economic Co-operation and Development(OECD)countries.This research used effective average tax(EAT),tax on personal income(TPI),tax on corporate profits(TCP),and tax on goods and services(TGS)as the variables of taxes,and employed secondary data from 38 OECD countries covering 2000–2021.The study also used Breusch-Pagan Lagrange Multiplier(LM),Pesaran Scaled LM,Bias-Corrected Scaled LM,and Pesaran Cross-sectional dependence(CSD)tests to analyze the existence of crosssectional dependency.Then,we established the stationarity of variables through second-generation panel unit root tests(Cross-sectional Augmented Dickey-Fuller(CADF)and Cross-sectional Im,Pesaran,and Shin(CIPS)),and confirmed the long-run cointegration of the variables by using secondgeneration panel cointegration test(Westerlund cointegration test).The results showed that EAT,TPI,TCP,and TGS are positively associated with SDGs.However,the change in TPI has a smaller effect on SDGs than the change in EAT or TCP or TGS.The result of panel causality indicated that EAT,TPI,and TGS have a unidirectional causal relationship with SDGs.The study also found that TCP has a bi-directional causal relationship with SDGs.Moreover,the finding indicated that the OECD countries need to focus on tax policies to achieve the 2030 Agenda for Sustainable Development.This study is based on the theory of optimal taxation(TOT),which suggests that tax systems should be designed to maximize social welfare.Finally,we suggests the importance of taking a comprehensive approach for the managers and policy-makers when analyzing the impact of taxes on SDGs.展开更多
Ribosome biogenesis in the nucleolus requires numerous nucleolar proteins and small non-coding RNAs.Among them is ribosome biogenesis factor Bmsl,which is highly conserved from yeast to human.In yeast,Bmsl initiates r...Ribosome biogenesis in the nucleolus requires numerous nucleolar proteins and small non-coding RNAs.Among them is ribosome biogenesis factor Bmsl,which is highly conserved from yeast to human.In yeast,Bmsl initiates ribosome biogenesis through recruiting Rcll to pre-ribosomes.However,little is known about the biological function of Bmsl in vertebrates.Here we report that Bmsl plays an essential role in zebrafish liver development.We identified a zebrafish bms1l^(sq163)mutant which carries a T to A mutation in the gene bmsl-like(bms1l).This mutation results in L^(152)to Q^(152)substitution in a GTPase motif in Bmsll.Surprisingly,bmsll^(sq163)mutation confers hypoplasia specifically in the liver,exocrine pancreas and intestine after 3 days post-fertilization(dpf).Consistent with the bmsll^(sq163)mutant phenotypes,whole-mount in situ hybridization(WISH)on wild type embryos showed that bmsll transcripts are abundant in the entire digestive tract and its accessory organs.Immunostaining for phospho-Histone 3(P-H3)and TUNEL assay revealed that impairment of hepatoblast proliferation rather than cell apoptosis is one of the consequences of bms1l(sq163)giving rise to an under-developed liver.Therefore,our findings demonstrate that Bmsll is necessary for zebrafish liver development.展开更多
Background： The health of chickens and the welfare of poultry industry are central to the efforts of addressing global food security. Therefore, it is essential to study chicken immunology to maintain and improve its...Background： The health of chickens and the welfare of poultry industry are central to the efforts of addressing global food security. Therefore, it is essential to study chicken immunology to maintain and improve its health and to find novel and sustainable solutions. This paper presents a study on investigation of the effect of Scutellaria baicalensis root（SBR） on the immune response of broiler chicken, especially on lymphocytes and heterophils reactivity, regarding their contribution to the development of immunity of the chickens.Methods： The 121-day-old Hubbard Hi-Y male broiler hybrids were randomly assigned to four treatment groups,three SBR supplemented groups（0.5, 1.0, and 1.5% of SBR） and one control group. Each treatment was replicated five times with six birds per replicate pen in a battery brooder. Blood was collected after 3-（rd） and 6-（th）wk of the experiment, and hemoglobin and hematocrit values were determined, as well as total leukocyte count and differential count were performed. Nitroblue tetrazolium test and phagocytosis assay as nonspecific immune parameters and humoral immune responses to the antigenic challenge by sheep red blood cells were performed.Moreover, the ability of peripheral blood lymphocytes to form radial segmentation（RS） of their nuclei was analyzed.Body weight and relative weight of spleen, liver, and bursa of Fabricius were recorded.Results： Results showed that mean heterophile/lymphocyte ratio increased in the SBR groups compared to the control group and the blood of the chickens showed lymphocytic depletion. The results also demonstrated that the relative weight of bursa of Fabricius and spleen in groups fed with SBR significantly decreased compared to the control group. This study also showed that the addition of SBR significantly inhibited the formation of RS of nuclei compared to some cytotoxic substances.Conclusion： We found that SBR supplementation should be carefully evaluated when given to poultry. The excess intake of SBR supplementation may cause immunologic inhibition and may negatively affect the development of immune organs. SBR has inhibited the formation of radial segmentation nuclei showing antimetastatic properties and also the phagocytosis of chicken heterophils.展开更多
A number of companies and organizations consider that it is necessary todevelop into learning organizations in order to meet the challenges of rapidly changing world. Aftera review on the literature on the learning or...A number of companies and organizations consider that it is necessary todevelop into learning organizations in order to meet the challenges of rapidly changing world. Aftera review on the literature on the learning organization, there is no question that the concept isboth attractive and complex. There appears to be more consensus about that becoming a learningorganization is more of a journey than a destination. Senge identifies five key disciplines thatKelp organization to become a learning organization, and the disciplines mean commitment, focus,and practice. In recent years the concept of the learning organization is translated into theeducation sector. Today, more than ever, more and more people see education as the highest form ofleverage to improve society. As the highest form of the education sector, universities must try todevelop into learning organizations. But the process will be neither easy nor swift, and we shouldview the process not as a task to be completed, but as the ongoing work. Effective change andimprovement can only happen by conducting long-term practice involving teachers, administrators,parents, and students who have a common vision and work and live with a learning culture.展开更多
Following fertilization in mammals, the zygote initiates the developmental program, which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages, which is defined as totipotency ...Following fertilization in mammals, the zygote initiates the developmental program, which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages, which is defined as totipotency (Condic, 2014). In mice,only zygotes and blastomeres of 2-cell stage embryos are considered totipotent, since they have the ability to develop into a full展开更多
Children with Developmental Coordination Disorder (DCD) often seem to possess a narrow repertoire of cognitive strategies. In particular, they have difficulties in learning and internalizing the rules and strategies t...Children with Developmental Coordination Disorder (DCD) often seem to possess a narrow repertoire of cognitive strategies. In particular, they have difficulties in learning and internalizing the rules and strategies that other people intuitively use to approach common everyday problems. As a result, they often appear to have organizational, planning, memory and learning difficulties. The article proposes using a Concept Map (CM) as a visual strategy to facilitate interaction between a child with DCD, his/her family and therapist, as reflected in Client Centred and cognitive approaches. The CM is used as a method of assisting the child to identify, develop and utilize cognitive strategies in order to manage daily tasks effectively, as a tool in organizing his own therapy and in order to encourage participation. A demonstration of the concept mapping usefulness is brought by a case report. Further uses of concept mapping as a useful strategy within the framework of intervention remain to be studied.展开更多
Cilia are indispensable for organ development and function,and their dysfunction causes a range of syndromic diseases known as ciliopathies,including obesity,cystic kidney disease,situs inversus,and male infertility(R...Cilia are indispensable for organ development and function,and their dysfunction causes a range of syndromic diseases known as ciliopathies,including obesity,cystic kidney disease,situs inversus,and male infertility(Reiter and Leroux,2017;Wallmeier et al.,2020).To date,over 180 ciliopathy-associated genes have been identified(Reiter and Leroux,2017),yet the underlying mechanisms remain poorly understood.展开更多
基金supported by grants from the 948 Project of the Ministry of Agriculture of China(2013-Z23)the Shanghai Municipal Science Commission of China(10391900100)
文摘The microscopic investigation of the floral development of sweet cherry(Prunus avium L.cv.Hongdeng)from a warm winter climate(Shanghai)and cold winter climate(Tai'an,Shandong Province,China)was conducted to explore the reason of low fruit set.The effect of hydrogen cyanamide(HCN)on floral development under warm winter conditions was also investigated.Trees grown in Shanghai with insufficient chilling accumulation exhibited little difference in the progression of microspore development compared to trees in Tai'an that accumulated adequate chilling,but showed substantial delays in ovule and embryo sac development.The growth of nucelli did not proceed beyond the macrospore mother cell and macrospore stages with abortion rates of 13,15 and 45%by 6,3 and 0 d before full bloom,respectively.These abnormalities in the ovule and embryo sac in the Shanghai-grown trees were eliminated by HCN application.These results suggest that chilling regulates the development of female floral organs in winter dormancy;therefore,insufficient chilling accumulation,causing abnormality of the female floral organs,restricts the cultivation of sweet cherry in warm winter regions.Interestingly,HCN application,which decreased the chilling requirements for Hongdeng,may be a potential strategy for sweet cherry cultivation in warm winter regions.
基金supported by the National Key Research and Developmental Program of China(2018YFA0801000)the National Natural Science Foundation of China(32473137,31721005)。
文摘Transcription factor IIIA(TFIIIA)is a zinc finger protein that facilitates the assembly of a transcription complex by recruiting transcription factors TFIIIB and TFIIIC,along with RNA polymerase III,to initiate the transcription of 5S rRNA genes.However,the effects of TFIIIA knockout in vertebrates remain unclear.To address this,we investigated the function of a homologous general transcription factor IIIAa,gtf3aa,identified as a maternal factor in zebrafish.During early embryonic development,gtf3aa expression initially increased and subsequently declined.At 12 h postfertilization,gtf3aa mRNA was detected at notably low levels in the embryo,whereas by three days postfertilization,its mRNA level gradually increased in the larvae.The gtf3aa was broadly expressed in various embryonic tissues of zebrafish,with higher expression levels observed in the brain,heart,liver,and muscle.Knockout of gtf3aa significantly suppressed somatic 5S rRNA transcription in early zebrafish embryos and larvae,resulting in a reduction in the number of mature monoribosomes and polyribosomes.The gtf3aa−/−larvae exhibited slow growth and delayed yolk absorption,along with impaired development of the eyes,heart,swim bladder,liver,and intestinal tissues.Additionally,expression of genes involved in metabolic signaling pathways,including the peroxisome proliferator-activated receptor pathway,was reduced.The gtf3aa−/−zebrafish did not survive beyond seven days postfertilization.This study offers preliminary insights into the role of gtf3aa in regulating somatic 5S rRNA transcription and embryonic organ development in zebrafish.
基金supported by the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province(2022SDZG05 to F.Kong,and B.Liu)the National Natural Science Foundation of China(32090064 to F.Kong,32330074 to B.Liu,and 32301874 to L.Wang)+1 种基金the Natural Science Foundation of Guangdong Province(2024A1515011314 to L.Wang)the Science and Technology Innovation Special Project of Modern Seed Industry(22326316D to F.Kong).
文摘The transition from vegetative to reproductive growth is a vital step for the reproductive success of plants.In Arabidopsis thaliana,LEAFY(LFY)plays crucial roles in inflorescence primordium and floral organ development,but little is known about the roles of its homologs in crop plants such as soybean(Glycine max).Here,we investigated the expression patterns and functions of the two LFY genes(LFY1 and LFY2)in soybean.Both genes were predominantly expressed in unopened flowers and the shoot apical meristem,with LFY2 having the higher transcript abundance.In an in situ hybridization assay,LFY genes produced strong signals in the floral meristem.We next generated lfy1 and lfy2 knockout lines.The lfy2 mutants showed obvious changes in floral organ morphology,but the lfy1 mutants showed no obvious changes in floral organ morphology or pod development.The lfy1 lfy2 double mutants displayed more serious defects in floral organ development than lfy2,resulting in complete sterility.Gene expression analysis revealed differences in expression of the A-class APETALA(AP)genes AP1a and AP1b in the double mutant lines.These results suggest that LFY2 plays an important role in floral organ formation in soybean by regulating the expression of homeotic genes.Our findings increase the understanding of floral development,which could be useful for flower designs during hybrid soybean breeding.
基金Supported by the National Natural Science Foundation of China(42172148,42172142)。
文摘Taking the Lower Silurian Longmaxi Formation shale in the Sichuan Basin as an example,this study employs atomic force microscopy-based infrared(AFM-IR)spectroscopy to analyze the submicron-scale molecular functional groups of different types and occurrences of organic matter.Combined with the quantitative evaluation of pore development via scanning electron microscopy(SEM),the response of organic pore formation and evolution mechanisms to chemical composition and structural evolution of organic matter in overmature marine shale is investigated.The results indicate that the AFM-IR spectra of graptolite periderms and pyrobitumen in shale are dominated by the stretching vibrations of conjugated C=C bonds in aromatic compounds at approximately 1600 cm-1,with weak absorption peaks near 1375,1450 and 1720 cm-1,corresponding to aliphatic chains and carbonyl/carboxyl functional groups.Overall,the AFM-IR structural indices(A and C factors)of organic matter show a strong correlation with visible porosity in shales of equivalent maturity.Lower A and C factor values correlate with enhanced development of organic pores,which is associated with the detachment of more aliphatic chains and oxygen-containing functional groups during thermal evolution.Pyrobitumen-clay mineral composites generally exhibit superior pore development,likely attributable to clay mineral dehydration participating in hydrocarbon generation reactions that promote the removal of more functional groups.Additionally,hydrocarbon generation within organic-clay composites during high-over mature stages may induce volumetric expansion,resulting in microfracturing and hydrocarbon expulsion.The associated higher hydrocarbon expulsion rates promote the formation of larger pores and fracture-shaped pores along the flake-shaped clay minerals.This study highlights that the research of submicron-scale molecular functional groups provides a deeper understanding of organic matter evolution and pores development mechanisms in overmature shales,thereby offering critical theoretical parameters for reservoir evaluation in shale oil and gas exploration.
文摘This research extends the literature on the environmental Phillips curve(EPC)and environmental Kuznets curve(EKC)by focusing on the 38 member economies of the Organization for Economic Co-operation and Development(OECD).Using panel data from 2000 to 2021,the study employs several econometric techniques,including fixed effects,feasible generalized least squares,two-stage least squares,and the generalized method of moments.Our primary findings reveal that unemployment has a significant negative impact on CO_(2)emissions,thereby supporting the validity of the EPC hypothesis within OECD countries.This suggests a trade-off between unemployment and reductions in CO_(2)emissions.Similarly,the results validate the EKC hypothesis,with further analysis indicating that the EKC exhibits an N-shaped curve-an important contribution to the literature on environmental dynamics in advanced economies.Additionally,the results show that both trade openness and renewable energy usage have significantly improved environmental quality in OECD economies.Finally,extensive causality testing identifies both one-way and two-way causal relationships among the key variables examined.These findings have important policy implications for the management of environmental quality and macroeconomic variables in the OECD context.
基金The National Basic Research Program(973 Program)of China under contract No.2010CB951203the National Natural Science Foundation of China under contract Nos 41206057,41576067,41376075 and 41576061
文摘Mangrove degradation must reduce carbon sequestration in recent years, thereby aggravating global warming.Thus, short-term impacts of human activity on mangrove ecosystems are cause for concern from local governments and scientists. Mangroves sediments can provide detailed records of mangrove species variation in the last one hundred years, based on detailed 210 Pb data. The study traced the history of mangrove development and its response to environmental change over the last 140 years in two mangrove swamps of Guangxi, Southwest China. Average sedimentation rates were calculated to be 0.48 cm/a and 0.56 cm/a in the Yingluo Bay and the Maowei Sea, respectively. Chemical indicators（δ13Corg and C：N） were utilized to trace the contribution of mangrove-derived organic matter（MOM） using a ternary mixing model. Simultaneous use of mangrove pollen can help to supplement some of these limitations in diagenetic/overlap of isotopic signatures. We found that vertical distribution of MOM was consistent with mangrove pollen, which could provide similar information for tracing mangrove ecosystems. Therefore, mangrove development was reconstructed and divided into three stages： flourishing, degradation and re-flourishing/re-degradation period. The significant degradation, found in the period of 1968–1998 and 1907–2007 in the Yingluo Bay and the Maowei Sea, respectively, corresponding to a rapid increase of reclamation area and seawall length, rather than climate change as recorded in the region.
文摘Dear Editor： I would like to congratulate Zhou et al.[1] on their study of the correlation between expression of lysyl oxidase-like 1 （LOX-1） and fibulin-5 （F5） in the car- dinal ligament tissue and pelvic organ prolapse （POP）. In their elegant work, they evaluated the levels of LOX-1 and F5 in connective tissue of the cardinal ligament in order to demonstrate signs of elastinopa- thy in women with POP. They stress the concept that several environmental risk factors could cause qualitative and quantitative changes in the connective tissue promoting POP. The above authors conclude that the specific mechanism of LOXL1 and F5 involved in the development of POP is unclear.
文摘The study elaborates and examines a method for evaluation of organic farming development according to 10 indicators estimated by experts＇ opinion. It could be used for making comparisons in different countries, regions, or even time periods on the basis of the 10 main indicators determined considering organic operators, organic area, organic production, organic farms＇ structure, resources, access to market, institutional support, education and training, science and technology, environmental protection, each one of them including sub-indicators. The proposed organic development index （ODI） could be used as a complex indicator for organic sector development embracing different and very significant aspects rather than only nowadays used organic area, percentage, sales, etc collected data for which are still questionable and difficult. The method was put into practice for six Balkan countries （Bulgaria, Serbia, Montenegro, Bosnia and Herzegovina, Turkey, and Romania）. The calculations of ODI show very low results--not well developed and competitive sector suffering the interference of international open markets. Turkey, Bulgaria, and Romania perform better while Western Balkan countries （Serbia, Montenegro, and Bosnia and Herzegovina） are still at the beginning of organic sector development.
文摘The Maanshan Mining Research Institute of theMinistry of Metallurgical Industry is a composite miningscientific research institution,one of the units with rightsof conferring master degree approved by the State Council,and enjoys foreign autonomous rights for import andexport,It has a staff of 1000,including 500 senior andintermediate technical people(42 professor-rank seniorengineers,and 39 enjoying government special subsidy). The institute has faculties including
文摘The Administrative Board of theLianyungang Development Zone isan executive administrative organimplementing unified leadership andmanagement over the development zone onbehalf of the Lianyungang People’sgovernment. According to the State’s lawsand regulations, the administrative board isresponsible for construction planning for thedevelopment zone, the execution of economicdevelopment programmes, the approval ofinvestment projects worth RMB30 millionor less, land use and building design, boostingpublic welfare in education, culture andsanitation, public security in the zone, andprotecting the legal rights of the
文摘Objective To study how to promote the smooth innovation and transformation of enterprises and enhance the market competitiveness of enterprises from the perspective of human resources organization development.In the wave of pharmaceutical industry reform,some entrepreneurs realize the importance of innovation and technology in the future.However,domestic pharmaceutical enterprises are still in the production-oriented stage.In the process of transformative innovation,pharmaceutical enterprises should not only rely on the support of scientific and technological and economic forces,but also need a suitable organizational change and many excellent talents as the source of development.Methods Through retrieving relevant literature and combined with the organizational development practice of X pharmaceutical enterprise,the organizational development model was explored in this paper.Results and Conclusion By forming a system of strategy,organization,talent,culture and mechanism,and building an organizational development model,we can improve the organizational atmosphere and organizational efficiency in the process of enterprise innovation and transformation,so as to enhance the competitiveness of enterprises in the market.
文摘Multiple ecological and socioeconomic problems have occurred worldwide,raising the awareness of sustainability.This study aims to examine the impact of taxes on Sustainable Development Goals(SDGs)in the context of Organization for Economic Co-operation and Development(OECD)countries.This research used effective average tax(EAT),tax on personal income(TPI),tax on corporate profits(TCP),and tax on goods and services(TGS)as the variables of taxes,and employed secondary data from 38 OECD countries covering 2000–2021.The study also used Breusch-Pagan Lagrange Multiplier(LM),Pesaran Scaled LM,Bias-Corrected Scaled LM,and Pesaran Cross-sectional dependence(CSD)tests to analyze the existence of crosssectional dependency.Then,we established the stationarity of variables through second-generation panel unit root tests(Cross-sectional Augmented Dickey-Fuller(CADF)and Cross-sectional Im,Pesaran,and Shin(CIPS)),and confirmed the long-run cointegration of the variables by using secondgeneration panel cointegration test(Westerlund cointegration test).The results showed that EAT,TPI,TCP,and TGS are positively associated with SDGs.However,the change in TPI has a smaller effect on SDGs than the change in EAT or TCP or TGS.The result of panel causality indicated that EAT,TPI,and TGS have a unidirectional causal relationship with SDGs.The study also found that TCP has a bi-directional causal relationship with SDGs.Moreover,the finding indicated that the OECD countries need to focus on tax policies to achieve the 2030 Agenda for Sustainable Development.This study is based on the theory of optimal taxation(TOT),which suggests that tax systems should be designed to maximize social welfare.Finally,we suggests the importance of taking a comprehensive approach for the managers and policy-makers when analyzing the impact of taxes on SDGs.
基金supported by the grants from the National Natural Science Foundation of China(NSFC)(No.31171391)to LJLan NSFC grant(No.30825025)to JRP and a grant from the National Research Foundation of Singapore(R-154-000-529-281)to YHH
文摘Ribosome biogenesis in the nucleolus requires numerous nucleolar proteins and small non-coding RNAs.Among them is ribosome biogenesis factor Bmsl,which is highly conserved from yeast to human.In yeast,Bmsl initiates ribosome biogenesis through recruiting Rcll to pre-ribosomes.However,little is known about the biological function of Bmsl in vertebrates.Here we report that Bmsl plays an essential role in zebrafish liver development.We identified a zebrafish bms1l^(sq163)mutant which carries a T to A mutation in the gene bmsl-like(bms1l).This mutation results in L^(152)to Q^(152)substitution in a GTPase motif in Bmsll.Surprisingly,bmsll^(sq163)mutation confers hypoplasia specifically in the liver,exocrine pancreas and intestine after 3 days post-fertilization(dpf).Consistent with the bmsll^(sq163)mutant phenotypes,whole-mount in situ hybridization(WISH)on wild type embryos showed that bmsll transcripts are abundant in the entire digestive tract and its accessory organs.Immunostaining for phospho-Histone 3(P-H3)and TUNEL assay revealed that impairment of hepatoblast proliferation rather than cell apoptosis is one of the consequences of bms1l(sq163)giving rise to an under-developed liver.Therefore,our findings demonstrate that Bmsll is necessary for zebrafish liver development.
基金supported by the Wroclaw Center for Biotechnology program KNOW(National Scientific Leadership Center)for the 2014–2018 award to BK
文摘Background： The health of chickens and the welfare of poultry industry are central to the efforts of addressing global food security. Therefore, it is essential to study chicken immunology to maintain and improve its health and to find novel and sustainable solutions. This paper presents a study on investigation of the effect of Scutellaria baicalensis root（SBR） on the immune response of broiler chicken, especially on lymphocytes and heterophils reactivity, regarding their contribution to the development of immunity of the chickens.Methods： The 121-day-old Hubbard Hi-Y male broiler hybrids were randomly assigned to four treatment groups,three SBR supplemented groups（0.5, 1.0, and 1.5% of SBR） and one control group. Each treatment was replicated five times with six birds per replicate pen in a battery brooder. Blood was collected after 3-（rd） and 6-（th）wk of the experiment, and hemoglobin and hematocrit values were determined, as well as total leukocyte count and differential count were performed. Nitroblue tetrazolium test and phagocytosis assay as nonspecific immune parameters and humoral immune responses to the antigenic challenge by sheep red blood cells were performed.Moreover, the ability of peripheral blood lymphocytes to form radial segmentation（RS） of their nuclei was analyzed.Body weight and relative weight of spleen, liver, and bursa of Fabricius were recorded.Results： Results showed that mean heterophile/lymphocyte ratio increased in the SBR groups compared to the control group and the blood of the chickens showed lymphocytic depletion. The results also demonstrated that the relative weight of bursa of Fabricius and spleen in groups fed with SBR significantly decreased compared to the control group. This study also showed that the addition of SBR significantly inhibited the formation of RS of nuclei compared to some cytotoxic substances.Conclusion： We found that SBR supplementation should be carefully evaluated when given to poultry. The excess intake of SBR supplementation may cause immunologic inhibition and may negatively affect the development of immune organs. SBR has inhibited the formation of radial segmentation nuclei showing antimetastatic properties and also the phagocytosis of chicken heterophils.
文摘A number of companies and organizations consider that it is necessary todevelop into learning organizations in order to meet the challenges of rapidly changing world. Aftera review on the literature on the learning organization, there is no question that the concept isboth attractive and complex. There appears to be more consensus about that becoming a learningorganization is more of a journey than a destination. Senge identifies five key disciplines thatKelp organization to become a learning organization, and the disciplines mean commitment, focus,and practice. In recent years the concept of the learning organization is translated into theeducation sector. Today, more than ever, more and more people see education as the highest form ofleverage to improve society. As the highest form of the education sector, universities must try todevelop into learning organizations. But the process will be neither easy nor swift, and we shouldview the process not as a task to be completed, but as the ongoing work. Effective change andimprovement can only happen by conducting long-term practice involving teachers, administrators,parents, and students who have a common vision and work and live with a learning culture.
基金supported by the National Natural Science Foundation of China (Nos. 31621004 and 81571356)the Key Research Projects of the Frontier Science of the Chinese Academy of Sciences (QYZDY-SSWSMC002)+1 种基金the Key Deployment Projects of the Chinese Academy of Sciences (ZDRW-ZS-2017-5)the Youth Innovation Promotion Association CAS (No. 2017113)
文摘Following fertilization in mammals, the zygote initiates the developmental program, which has a transient capacity to generate cell types of both embryonic and extraembryonic lineages, which is defined as totipotency (Condic, 2014). In mice,only zygotes and blastomeres of 2-cell stage embryos are considered totipotent, since they have the ability to develop into a full
文摘Children with Developmental Coordination Disorder (DCD) often seem to possess a narrow repertoire of cognitive strategies. In particular, they have difficulties in learning and internalizing the rules and strategies that other people intuitively use to approach common everyday problems. As a result, they often appear to have organizational, planning, memory and learning difficulties. The article proposes using a Concept Map (CM) as a visual strategy to facilitate interaction between a child with DCD, his/her family and therapist, as reflected in Client Centred and cognitive approaches. The CM is used as a method of assisting the child to identify, develop and utilize cognitive strategies in order to manage daily tasks effectively, as a tool in organizing his own therapy and in order to encourage participation. A demonstration of the concept mapping usefulness is brought by a case report. Further uses of concept mapping as a useful strategy within the framework of intervention remain to be studied.
基金supported by grants from the National Key Research and Development Program of China(2019YFA0802704)the National Natural Science Foundation of China(31771620)+2 种基金the Natural Science Foundation of Chongqing,China(CSTB2022NSCQMSX1424)Research Startup Fund of Southwest University(SWU117064)Open Research Fund of National Health Commission Key Laboratory of Birth Defects Prevention&Henan Key Laboratory of Population Defects Prevention(ZD202302)。
文摘Cilia are indispensable for organ development and function,and their dysfunction causes a range of syndromic diseases known as ciliopathies,including obesity,cystic kidney disease,situs inversus,and male infertility(Reiter and Leroux,2017;Wallmeier et al.,2020).To date,over 180 ciliopathy-associated genes have been identified(Reiter and Leroux,2017),yet the underlying mechanisms remain poorly understood.
