Naf1(Nef-associated factor 1)is a host protein that interacts with human immunodeficiency virus type 1(HIV-1)Nef protein.We and others have previously demonstrated that Naf1 restricts HIV-1 infection of T-lymphocytes....Naf1(Nef-associated factor 1)is a host protein that interacts with human immunodeficiency virus type 1(HIV-1)Nef protein.We and others have previously demonstrated that Naf1 restricts HIV-1 infection of T-lymphocytes.Myeloid cells are targets for HIV infection,but Naf1 expression in myeloid cells and whether it also regulates HIV infection in these cells are not yet identified.In this study,we found that Naf1 had a higher expression in CD14þmonocytes than in monocyte-derived dendritic cells(MDDCs),and its expression in both types of cells could be induced by HIV-1 gp120 glycoproteins or viral particles.Importantly,the expression of Naf1 restricted HIV-1 infection in monocytes and MDDCs.Functional investigation showed that both the constitutive and the induced expression of Naf1 inhibited NF-κB signaling in MDDCs and reduced the basal level or LPS(Lipopolysaccharide)-stimulated production of cytokines.Moreover,Naf1 reduced the expression of ICAM-1(intercellular cell adhesion molecule-1)on MDDCs and compromised their capacity to prime the activation of resting CD4^(+)T cells in co-culture.In light of the essential role of NF-κB signaling for HIV-1 transcription,Naf1-mediated inhibition of NF-κB signaling may hinder a robust viral replication in MDDCs and help maintain viral persistence.Furthermore,virus-induced Naf1 expression in MDDCs may diminish the cross-talk between DC(dendritic cell)and T cells,hence suppressing the activation of antiviral immune responses.Taken together,we identified the new function of Naf1 in myeloid cells.Those findings may facilitate the understanding for the host restriction of HIV-1 infection in myeloid cells.展开更多
In the present research,the NaF assisted plasma electrolytic oxidation(PEO)is designed to fabricate the high-content ZnO nanoparti-cles doped coating on AZ31B alloy.The microstructure,phase constituents and corrosion ...In the present research,the NaF assisted plasma electrolytic oxidation(PEO)is designed to fabricate the high-content ZnO nanoparti-cles doped coating on AZ31B alloy.The microstructure,phase constituents and corrosion behavior of the PEO coatings are investigated systematically.The results reveal that the introduction of NaF promotes the formation of MgF2 nanophases in the passivation layer on Mg alloy,decreasing the breakdown voltage and discharge voltage.As a result,the continuous arcing caused by high discharge voltage is alleviated.With the increasing of NaF content,the Zn content in the PEO coating is enhanced and the pore size in the coating is decreased correspondingly.Due to the high-content ZnO doping,the PEO coating protected AZ31B alloy demonstrates the better corrosion resistance.Compared with the bare AZ31B alloy,the high-content ZnO doped PEO coated sample shows an increased corrosion potential from-1.465 V to-1.008 V,a decreased corrosion current density from 3.043×10^(-5) A·cm^(-2) to 3.960×10^(-8) A·cm^(-2) and an increased charge transfer resistance from 1.213×10^(2) ohm·cm^(2) to 2.598×10^(5) ohm·cm^(2).Besides,the high-content ZnO doped PEO coated sample also has the excellent corrosion resistance in salt solution,exhibiting no obvious corrosion after more than 2000 h neutral salt spraying and 28 days’immersion testing.The improved corrosion resistance can be ascribed to the relative uniform distribution of ZnO in PEO coating which can transform to Zn(OH)2 and form a continuous protective layer along the corrosion interface.展开更多
The electrical conductivity of NaF-AlF3-CaF2-Al2O3-ZrO2 system was studied by a tube-type cell with fixed cell constant. The results show that the electrical conductivity of NaF-AlF3-3%Al2O3-3%CaF2-ZrO2 molten salt sy...The electrical conductivity of NaF-AlF3-CaF2-Al2O3-ZrO2 system was studied by a tube-type cell with fixed cell constant. The results show that the electrical conductivity of NaF-AlF3-3%Al2O3-3%CaF2-ZrO2 molten salt system decreases with increase of ZrO2 content in an interval of 0-5%. The increase of 1%ZrO2 results in a corresponding electrical conductivity decrease of 0.02 S/cm, and the equivalent conductivity increases with the increase of molar ratio of NaF to AlF3. When the temperature increases by 1 °C, the electrical conductivity increases by 0.004 S/cm. At last, the regression equations of electrical conductivity relative to temperature and ZrO2 are obtained by quadratic regression analysis.展开更多
The Eu-doped SiO2-B2O3-NaF glass was prepared by sol-gel process, using tetraethoxy Silicane, boric acid and sodium fluoride as starting materials, 0.10 mol·L-1 EuCl3 solution as the dopant. The luminescent prope...The Eu-doped SiO2-B2O3-NaF glass was prepared by sol-gel process, using tetraethoxy Silicane, boric acid and sodium fluoride as starting materials, 0.10 mol·L-1 EuCl3 solution as the dopant. The luminescent properties of Eu3+ doped SiO2-B2O3-NaF phosphors were investigated. The phosphors showed prominent luminescence in pink, the strong emission of Eu3+ comes from electronic transition of 5D0-7F1(591 nm)and 5D0-7F2(615 nm),which derived from two transition modes of magnetic-dipole and electric-dipole .The peak intensity of 591nm in SiO2-B2O3-NaF matrix is much stronger than it in the other matrixes, it means that SiO2-B2O3-NaF has sensitization on the transition of 5D0-7F1 (Eu3+). If there are broad bonds in the range of 275~380 nm in the excitation spectrum of Eu3+ -doped SiO2-B2O3-NaF glass, the emission peak intensity should be intensified. It is because the electron migration CT band of O2--Eu3+. For all Eu3+ concentrations used, the investigation found that when the mass of fraction got to 29.19×10-3, the luminescence intensity reached the summit. And there is a phenomenon of concentration quenching. Investigation with the same concentration of Eu3+ at different annealed temperature, we found that the sample annealed at 400 ℃, the luminescence intensity achieved its maximum value, and Eu3+ in this matrix had a phenomenon of temperature quenching. The structural characterization of these luminescent materials was carried by used XRD and TEM. The result showed that the phosphor was in amorphous phase.展开更多
基金supported by the National Natural Science Foundation of China(82172242)the Natural Science Foundation of Guangdong(2022A1515012053)the projects from Guangzhou Municipal Science and Technology Bureau(2023A04J1075).
文摘Naf1(Nef-associated factor 1)is a host protein that interacts with human immunodeficiency virus type 1(HIV-1)Nef protein.We and others have previously demonstrated that Naf1 restricts HIV-1 infection of T-lymphocytes.Myeloid cells are targets for HIV infection,but Naf1 expression in myeloid cells and whether it also regulates HIV infection in these cells are not yet identified.In this study,we found that Naf1 had a higher expression in CD14þmonocytes than in monocyte-derived dendritic cells(MDDCs),and its expression in both types of cells could be induced by HIV-1 gp120 glycoproteins or viral particles.Importantly,the expression of Naf1 restricted HIV-1 infection in monocytes and MDDCs.Functional investigation showed that both the constitutive and the induced expression of Naf1 inhibited NF-κB signaling in MDDCs and reduced the basal level or LPS(Lipopolysaccharide)-stimulated production of cytokines.Moreover,Naf1 reduced the expression of ICAM-1(intercellular cell adhesion molecule-1)on MDDCs and compromised their capacity to prime the activation of resting CD4^(+)T cells in co-culture.In light of the essential role of NF-κB signaling for HIV-1 transcription,Naf1-mediated inhibition of NF-κB signaling may hinder a robust viral replication in MDDCs and help maintain viral persistence.Furthermore,virus-induced Naf1 expression in MDDCs may diminish the cross-talk between DC(dendritic cell)and T cells,hence suppressing the activation of antiviral immune responses.Taken together,we identified the new function of Naf1 in myeloid cells.Those findings may facilitate the understanding for the host restriction of HIV-1 infection in myeloid cells.
基金supported by the 2022 Shenzhen sustainable supporting funds for colleges and universities(20220810143642004)Shenzhen Basic Research Project(JCYJ20200109144608205 and JCYJ20210324120001003)+5 种基金Peking University Shenzhen Graduate School Research Startup Fund of Introducing Talent(No.1270110273)Shenzhen postdoctoral research fund project after outbound(No.2129933651)Shenzhen-Hong Kong Research and Development Fund(No.SGDX20201103095406024)City University of Hong Kong Strategic Research Grants(SRG)(Nos.7005264 and 7005505)Guangdong-Hong Kong Technology Cooperation Funding Scheme(TCFS)(No.GHP/085/18SZ)IER Foundation(IERF2020001 and IERF2019002).
文摘In the present research,the NaF assisted plasma electrolytic oxidation(PEO)is designed to fabricate the high-content ZnO nanoparti-cles doped coating on AZ31B alloy.The microstructure,phase constituents and corrosion behavior of the PEO coatings are investigated systematically.The results reveal that the introduction of NaF promotes the formation of MgF2 nanophases in the passivation layer on Mg alloy,decreasing the breakdown voltage and discharge voltage.As a result,the continuous arcing caused by high discharge voltage is alleviated.With the increasing of NaF content,the Zn content in the PEO coating is enhanced and the pore size in the coating is decreased correspondingly.Due to the high-content ZnO doping,the PEO coating protected AZ31B alloy demonstrates the better corrosion resistance.Compared with the bare AZ31B alloy,the high-content ZnO doped PEO coated sample shows an increased corrosion potential from-1.465 V to-1.008 V,a decreased corrosion current density from 3.043×10^(-5) A·cm^(-2) to 3.960×10^(-8) A·cm^(-2) and an increased charge transfer resistance from 1.213×10^(2) ohm·cm^(2) to 2.598×10^(5) ohm·cm^(2).Besides,the high-content ZnO doped PEO coated sample also has the excellent corrosion resistance in salt solution,exhibiting no obvious corrosion after more than 2000 h neutral salt spraying and 28 days’immersion testing.The improved corrosion resistance can be ascribed to the relative uniform distribution of ZnO in PEO coating which can transform to Zn(OH)2 and form a continuous protective layer along the corrosion interface.
基金Project (2007CB210305) supported by the National Basic Research Program of ChinaProject (51074045) supported by the National Natural Science Foundation of China
文摘The electrical conductivity of NaF-AlF3-CaF2-Al2O3-ZrO2 system was studied by a tube-type cell with fixed cell constant. The results show that the electrical conductivity of NaF-AlF3-3%Al2O3-3%CaF2-ZrO2 molten salt system decreases with increase of ZrO2 content in an interval of 0-5%. The increase of 1%ZrO2 results in a corresponding electrical conductivity decrease of 0.02 S/cm, and the equivalent conductivity increases with the increase of molar ratio of NaF to AlF3. When the temperature increases by 1 °C, the electrical conductivity increases by 0.004 S/cm. At last, the regression equations of electrical conductivity relative to temperature and ZrO2 are obtained by quadratic regression analysis.
文摘The Eu-doped SiO2-B2O3-NaF glass was prepared by sol-gel process, using tetraethoxy Silicane, boric acid and sodium fluoride as starting materials, 0.10 mol·L-1 EuCl3 solution as the dopant. The luminescent properties of Eu3+ doped SiO2-B2O3-NaF phosphors were investigated. The phosphors showed prominent luminescence in pink, the strong emission of Eu3+ comes from electronic transition of 5D0-7F1(591 nm)and 5D0-7F2(615 nm),which derived from two transition modes of magnetic-dipole and electric-dipole .The peak intensity of 591nm in SiO2-B2O3-NaF matrix is much stronger than it in the other matrixes, it means that SiO2-B2O3-NaF has sensitization on the transition of 5D0-7F1 (Eu3+). If there are broad bonds in the range of 275~380 nm in the excitation spectrum of Eu3+ -doped SiO2-B2O3-NaF glass, the emission peak intensity should be intensified. It is because the electron migration CT band of O2--Eu3+. For all Eu3+ concentrations used, the investigation found that when the mass of fraction got to 29.19×10-3, the luminescence intensity reached the summit. And there is a phenomenon of concentration quenching. Investigation with the same concentration of Eu3+ at different annealed temperature, we found that the sample annealed at 400 ℃, the luminescence intensity achieved its maximum value, and Eu3+ in this matrix had a phenomenon of temperature quenching. The structural characterization of these luminescent materials was carried by used XRD and TEM. The result showed that the phosphor was in amorphous phase.
