摘要
本研究旨在探究^(18)F-NaF micro PET/CT显像对急性痛风性关节炎(AGA)小鼠模型的诊断价值。选取雄性Balb/c小鼠正常组(A组)3只,AGA模型组(B组)18只;A组与模型组B组(B_(1h)、B_(3h)、B_(6h)、B_(8h)、B_(12h)、B_(24h))均于注射显像剂40 min后进行micro PET/CT显像。各组小鼠均行血常规分析、血尿酸检测和踝关节病理组织活检。本研究结果显示:B组血常规炎性细胞数均高于A组,其中B_(6h)、B_(8h)组与A组差异有统计学意义(P<0.05)。^(18)F-NaF micro PET/CT显像示B组右侧踝关节可见异常显像剂聚集灶,但病灶处CT并未见骨质破坏;A组左侧踝关节均未见明显显像剂异常聚集灶。B组右侧与左侧踝关节最大标准化摄取值(SUVmax)比值(R/LSUVmax)均高于A组,其中B_(6h)组与A组差异有统计学意义(P<0.05)。R/LSUVmax比值与血常规白细胞数、中性粒细胞数及病理组织镜下炎性细胞数呈正相关(分别为R=0.79,P<0.01;R=0.72,P<0.01;R=0.79,P<0.01)。综上,^(18)F-NaF micro PET/CT显像可早期检测AGA骨代谢改变,可实现AGA病理生理动态改变的可视化监测。
This study aims to investigate the diagnostic value of ^(18)F-NaF micro PET/CT imaging in mouse models of acute gouty arthritis(AGA).Three male Balb/c mice were designated as the normal control group(Group A),and 18 male Balb/c mice were used to establish the AGA model(Group B).Group A and model groups B(B1h,B3h,B_(6h),B_(8h),B_(12h),B_(24h))underwent micro PET/CT imaging 40 minutes after injection of the radiotracer.All groups of mice underwent complete blood count,blood uric acid testing,and pathological biopsy of the ankle joint.The results showed that the counts of inflammatory cells in the blood routine of Group B were higher than those of Group A,and there were statistically significant differences between Group B_(6h) and B_(8h) compared to Group A(P<0.05).^(18)F-NaF micro PET/CT imaging revealed abnormal tracer accumulation in the right ankle joints of group B,but no bone destruction were observed on CT at the lesion sites;In group A,there was no obvious abnormal gathering of tracer in the left ankle joint.The ratios of maximum standardized uptake value(SUVmax)of the right and left ankle joints(R/LSUVmax)in Group B were higher than those in Group A,and the difference between Group B_(6h) and Group A was statistically significant(P<0.05).The R/LSUVmax ratios were positively correlated with the counts of white blood cells and neutrophils in the blood routine and microscopic inflammatory cells(R=0.79,P<0.01;R=0.72,P<0.01;R=0.79,P<0.01,respectively).Overall,^(18)F-NaF micro PET/CT imaging can detect early bone metabolism changes in AGA and visually monitor its dynamic pathophysiological progression.
作者
游智骁
朱焓钰
石野宽
李沛琳
黄晓红
张曾
李素平
游金辉
YOU Zhixiao;ZHU Hanyu;SHI Yekuan;LI Peilin;HUANG Xiaohong;ZHANG Zeng;LI Suping;YOU Jinhui(North Sichuan Medical College Sichuan Key Laboratory of Medical Imaging,The Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,P.R.China;Suining Third People’s Hospital,Suining,Sichuan 629000,P.R.China)
出处
《生物医学工程学杂志》
北大核心
2025年第6期1251-1256,共6页
Journal of Biomedical Engineering
基金
四川省自然科学基金(2024NSFSC0668)
川北医学院青年项目(自然科学类:CBY22-QNA60)。
