Chromosome segmentation is the most important step in the automatic chromosome analysis system. Since in almost every metaphase image partial touching and overlapping of chromosomes are a common phenomenon, how to sep...Chromosome segmentation is the most important step in the automatic chromosome analysis system. Since in almost every metaphase image partial touching and overlapping of chromosomes are a common phenomenon, how to separate these chromosomes correctly is a difficult yet vital problem. A number of attempts have been made to deal with this problem. This paper is focused on these attempts. Some algorithms are investigated. The principle and the realization of these algorithms are analyzed. Results of these algorithms are compared and discussed.展开更多
Ticks are a major parasite on the Qinghai-Tibet Plateau,western China,and represent an economic burden to agriculture and animal husbandry.Despite research on tick-borne pathogens that threaten humans and animals,the ...Ticks are a major parasite on the Qinghai-Tibet Plateau,western China,and represent an economic burden to agriculture and animal husbandry.Despite research on tick-borne pathogens that threaten humans and animals,the viromes of dominant tick species in this area remain unknown.In this study,we collected Dermacentor nuttalli ticks near Qinghai Lake and identified 13 viruses belonging to at least six families through metagenomic sequencing.Four viruses were of high abundance in pools,including Xınjiang tick-associated virus 1(XJTAV1),and three novel viruses:Qinghai Lake virus 1,Qinghai Lake virus 2(QHLV1,and QHLV2,unclassified),and Qinghai Lake virus 3(QHLV3,genus Uukuvirus of family Phenuiviridae in order Bunyavirales),which lacks the M segment.The minimum infection rates of the four viruses in the tick groups were 8.2%,49.5%,6.2%,and 24.7%,respectively,suggesting the prevalence of these viruses in D.nuttalli ticks.A putative M segment of QHLV3 was identified from the next-generation sequencing data and further characterized for its signal peptide cleavage site,N-glycosylation,and transmembrane region.Furthermore,we probed the L,M,and S segments of other viruses from sequencing data of other tick pools by using the putative M segment sequence of QHLV3.By revealing the viromes of D.nuttalli ticks,this study enhances our understanding of tick-borne viral communities in highland regions.The putative M segment identified in a novel uukuvirus suggests that previously identified uukuviruses without M segments should have had the same genome organization as typical bunyaviruses.These findings will facilitate virus discovery and our understanding of the phylogeny of tick-borne uukuviruses.展开更多
目的探讨急性ST段抬高型心肌梗死(ASTEMI)患者血清免疫相关鸟苷三磷酸酶M蛋白(IRGM)和多配体蛋白聚糖-1(Syndecan-1)水平表达对经皮冠状动脉介入治疗(PCI)术后预后的影响。方法选取2021年1月~2023年1月上海交通大学医学院苏州九龙医院...目的探讨急性ST段抬高型心肌梗死(ASTEMI)患者血清免疫相关鸟苷三磷酸酶M蛋白(IRGM)和多配体蛋白聚糖-1(Syndecan-1)水平表达对经皮冠状动脉介入治疗(PCI)术后预后的影响。方法选取2021年1月~2023年1月上海交通大学医学院苏州九龙医院收治的ASTEMI患者135例(ASTEMI组),PCI治疗三个月后分为不良预后组(n=37)和良好预后组(n=98);另选取同期65例体检健康者(对照组)。采用酶联免疫吸附法(ELISA)检测血清IRGM,Syndecan-1水平,Logistic回归分析ASTEMI患者PCI术后不良预后的影响因素,并绘制受试者工作特征(ROC)曲线评价血清IRGM,Syndecan-1水平对ASTEMI患者PCI术后不良预后的预测价值。结果与对照组比较,ASTEMI组血清IRGM(6.17±2.50ng/ml vs 2.59±0.94ng/ml),Syndecan-1(420.97±123.65ng/ml vs 278.89±43.06ng/ml)水平更高,差异具有统计学意义(t=14.628,11.932,均P<0.01)。随访三个月,135例ASTEMI患者PCI术后不良预后发生率为27.41%(37/135);不良预后组患者年龄、KILLIP分级≥Ⅱ级占比、Gensini评分、白细胞计数、LDL-C,IRGM,Syndecan-1水平高于良好预后组,LVEF分数低于良好预后组,差异具有统计学意义(χ^(2)/t/Z=2.119~8.042,均P<0.05)。ASTEMI患者PCI术后KILLIP分级≥Ⅱ级、Gensini评分高、IRGM高表达、Syndecan-1高表达为不良预后的独立危险因素;LVEF分数高为独立保护因素(Waldχ^(2)=4.225~11.413,均P<0.05)。血清IRGM,Syndecan-1水平联合预测ASTEMI患者PCI术后不良预后的曲线下面积高于IRGM,Syndecan-1单独预测,差异具有统计学意义(Z=3.400,2.905,均P<0.05)。结论血清IRGM,Syndecan-1水平高是ASTEMI患者PCI术后不良预后的独立危险因素,血清IRGM,Syndecan-1水平联合对其有较高的预测价值。展开更多
文摘Chromosome segmentation is the most important step in the automatic chromosome analysis system. Since in almost every metaphase image partial touching and overlapping of chromosomes are a common phenomenon, how to separate these chromosomes correctly is a difficult yet vital problem. A number of attempts have been made to deal with this problem. This paper is focused on these attempts. Some algorithms are investigated. The principle and the realization of these algorithms are analyzed. Results of these algorithms are compared and discussed.
基金supported by the National Key R&D Program of China(2022YFC2305100,2021YFC2300900)the Key Deployment Projects of the Chinese Academy of Sciences(KJZD-SW-L11)+2 种基金the Youth Project of the Wuhan Institute of Virology,Chinese Academy of Sciences(2023QNTJ-03)the Biological Resources Program,Chinese Academy of Sciences(KFJ-BRP-017-74)the National Basic Science Data Sharing Service Platform(NBSDC-DB-13).
文摘Ticks are a major parasite on the Qinghai-Tibet Plateau,western China,and represent an economic burden to agriculture and animal husbandry.Despite research on tick-borne pathogens that threaten humans and animals,the viromes of dominant tick species in this area remain unknown.In this study,we collected Dermacentor nuttalli ticks near Qinghai Lake and identified 13 viruses belonging to at least six families through metagenomic sequencing.Four viruses were of high abundance in pools,including Xınjiang tick-associated virus 1(XJTAV1),and three novel viruses:Qinghai Lake virus 1,Qinghai Lake virus 2(QHLV1,and QHLV2,unclassified),and Qinghai Lake virus 3(QHLV3,genus Uukuvirus of family Phenuiviridae in order Bunyavirales),which lacks the M segment.The minimum infection rates of the four viruses in the tick groups were 8.2%,49.5%,6.2%,and 24.7%,respectively,suggesting the prevalence of these viruses in D.nuttalli ticks.A putative M segment of QHLV3 was identified from the next-generation sequencing data and further characterized for its signal peptide cleavage site,N-glycosylation,and transmembrane region.Furthermore,we probed the L,M,and S segments of other viruses from sequencing data of other tick pools by using the putative M segment sequence of QHLV3.By revealing the viromes of D.nuttalli ticks,this study enhances our understanding of tick-borne viral communities in highland regions.The putative M segment identified in a novel uukuvirus suggests that previously identified uukuviruses without M segments should have had the same genome organization as typical bunyaviruses.These findings will facilitate virus discovery and our understanding of the phylogeny of tick-borne uukuviruses.
文摘目的探讨急性ST段抬高型心肌梗死(ASTEMI)患者血清免疫相关鸟苷三磷酸酶M蛋白(IRGM)和多配体蛋白聚糖-1(Syndecan-1)水平表达对经皮冠状动脉介入治疗(PCI)术后预后的影响。方法选取2021年1月~2023年1月上海交通大学医学院苏州九龙医院收治的ASTEMI患者135例(ASTEMI组),PCI治疗三个月后分为不良预后组(n=37)和良好预后组(n=98);另选取同期65例体检健康者(对照组)。采用酶联免疫吸附法(ELISA)检测血清IRGM,Syndecan-1水平,Logistic回归分析ASTEMI患者PCI术后不良预后的影响因素,并绘制受试者工作特征(ROC)曲线评价血清IRGM,Syndecan-1水平对ASTEMI患者PCI术后不良预后的预测价值。结果与对照组比较,ASTEMI组血清IRGM(6.17±2.50ng/ml vs 2.59±0.94ng/ml),Syndecan-1(420.97±123.65ng/ml vs 278.89±43.06ng/ml)水平更高,差异具有统计学意义(t=14.628,11.932,均P<0.01)。随访三个月,135例ASTEMI患者PCI术后不良预后发生率为27.41%(37/135);不良预后组患者年龄、KILLIP分级≥Ⅱ级占比、Gensini评分、白细胞计数、LDL-C,IRGM,Syndecan-1水平高于良好预后组,LVEF分数低于良好预后组,差异具有统计学意义(χ^(2)/t/Z=2.119~8.042,均P<0.05)。ASTEMI患者PCI术后KILLIP分级≥Ⅱ级、Gensini评分高、IRGM高表达、Syndecan-1高表达为不良预后的独立危险因素;LVEF分数高为独立保护因素(Waldχ^(2)=4.225~11.413,均P<0.05)。血清IRGM,Syndecan-1水平联合预测ASTEMI患者PCI术后不良预后的曲线下面积高于IRGM,Syndecan-1单独预测,差异具有统计学意义(Z=3.400,2.905,均P<0.05)。结论血清IRGM,Syndecan-1水平高是ASTEMI患者PCI术后不良预后的独立危险因素,血清IRGM,Syndecan-1水平联合对其有较高的预测价值。
