Objectives To compare respiratory parameters of peripheral blood mononuclear cell mitochondria and iron metabolism indicators in patients with different NYHA functional classes of ischemic heart failure(HF).Methods Th...Objectives To compare respiratory parameters of peripheral blood mononuclear cell mitochondria and iron metabolism indicators in patients with different NYHA functional classes of ischemic heart failure(HF).Methods This single center, prospective, non-blinded study enrolled 20 patients with diagnosed chronic HF of ischemic genesis with reduced and mildly reduced left ventricle ejection fraction. The maximum oxygen consumption at the peak of the exercise test(VO2peak), iron metabolism parameters and respiratory activity of peripheral blood mononuclear cell mitochondria were assessed.Results Among the patients, a half of individuals were diagnosed with iron deficiency. Subgroups of patients with different HF severity did not significant differ in VO2peak(P=0.209), serum iron(P=0.468) and ferritin(P=0.235) levels. But there was a trend in increasing in these parameters with increasing NYHA HF functional class. Respiratory control coefficient(RC) in NADdependent and FAD-dependent mitochondrial oxidation were lower in patients with NYHA HF Ⅲ functional class compared to individuals with NYHA HF I functional class(P=0.028 and P=0.040, respectively). Serum iron(P=0.026), ferritin(P=0.045)levels, transferrin saturation(P=0.006) were negatively correlated with RC in NAD-dependent mitochondrial oxidation.Conclusions In aggravation of ischemic HF NYHA FC, there is a decrease in RC of PBMC mitochondria during the oxidation of NAD-dependent and FAD-dependent substrates. In the whole sample, patients with laboratory-confirmed iron deficiency accounted a half of the total number. Iron metabolism parameters had a paradoxical inverse relationship with the level of RC in PBMC mitochondria of patients with HF.展开更多
Forty soils from top layer (0-20 cm) were sampled in different regions of China and Kjeldahl, HF-Kjeldahl and double treatment methods were used to determine total N, total N plus fixed ammonium, and total N and the r...Forty soils from top layer (0-20 cm) were sampled in different regions of China and Kjeldahl, HF-Kjeldahl and double treatment methods were used to determine total N, total N plus fixed ammonium, and total N and the residual fixed ammonium left in soil after determination of total N, respectively, to evaluate if Kjeldahl’s method could include the fixed N by soil minerals. The fixed N by soil minerals was measured by Silva-Bremner procedure to make comparison. Results showed that total N determined by Kjeldahl’s method averaged 1.622 g kg-1, while that by HF- Kjeldahl’s method 1.633 g kg-1, and that by double procedure 1.666 g kg-1. Obviously results obtained by the last two methods, particularly the double treatment method, were higher than Kjeldahl’s, showing that Kjeldahl’s method could not or not fully release N fixed by 2:1 minerals in soil, and therefore the determined results would not be the true “total N” for soils that contained large amount of the fixed N. The mineral fixedN averaged 166 mg kg-1, accounting for 10.1% of the total N while the residual fixed N amounted to 30.4 mg kg-1, equivalent to 1.9% of the total N or 18.3% of the total fixed N. The residual fixed N was correlated neither to organic matter nor to total N, but closely related to the total fixed N with a correlation coefficient of 0.598 (n=40), showing that the fixed N was the sole source of the residues. Soils having high residues of the fixed N were just those containing high fixed N, and soils containing high fixed N were just those containing high amount of 2:1 minerals. As a result, Kjeldahl’s method could not give a true value of the total N for such soils. However, for those containing small or little amount of 2:1 minerals, there was no significant difference in results measured by these methods.展开更多
基金supported by Russian Science Foundation,RSF 23-75-00009(part of the study corresponding to finding 1)Part of the study corresponding to finding 2 was carried out within the state assignment,FSR No.:122020300045-5(03.02.2022).
文摘Objectives To compare respiratory parameters of peripheral blood mononuclear cell mitochondria and iron metabolism indicators in patients with different NYHA functional classes of ischemic heart failure(HF).Methods This single center, prospective, non-blinded study enrolled 20 patients with diagnosed chronic HF of ischemic genesis with reduced and mildly reduced left ventricle ejection fraction. The maximum oxygen consumption at the peak of the exercise test(VO2peak), iron metabolism parameters and respiratory activity of peripheral blood mononuclear cell mitochondria were assessed.Results Among the patients, a half of individuals were diagnosed with iron deficiency. Subgroups of patients with different HF severity did not significant differ in VO2peak(P=0.209), serum iron(P=0.468) and ferritin(P=0.235) levels. But there was a trend in increasing in these parameters with increasing NYHA HF functional class. Respiratory control coefficient(RC) in NADdependent and FAD-dependent mitochondrial oxidation were lower in patients with NYHA HF Ⅲ functional class compared to individuals with NYHA HF I functional class(P=0.028 and P=0.040, respectively). Serum iron(P=0.026), ferritin(P=0.045)levels, transferrin saturation(P=0.006) were negatively correlated with RC in NAD-dependent mitochondrial oxidation.Conclusions In aggravation of ischemic HF NYHA FC, there is a decrease in RC of PBMC mitochondria during the oxidation of NAD-dependent and FAD-dependent substrates. In the whole sample, patients with laboratory-confirmed iron deficiency accounted a half of the total number. Iron metabolism parameters had a paradoxical inverse relationship with the level of RC in PBMC mitochondria of patients with HF.
基金the National Natural Sci。ence Foundation of China(30230230,30070429 , 40201028)
文摘Forty soils from top layer (0-20 cm) were sampled in different regions of China and Kjeldahl, HF-Kjeldahl and double treatment methods were used to determine total N, total N plus fixed ammonium, and total N and the residual fixed ammonium left in soil after determination of total N, respectively, to evaluate if Kjeldahl’s method could include the fixed N by soil minerals. The fixed N by soil minerals was measured by Silva-Bremner procedure to make comparison. Results showed that total N determined by Kjeldahl’s method averaged 1.622 g kg-1, while that by HF- Kjeldahl’s method 1.633 g kg-1, and that by double procedure 1.666 g kg-1. Obviously results obtained by the last two methods, particularly the double treatment method, were higher than Kjeldahl’s, showing that Kjeldahl’s method could not or not fully release N fixed by 2:1 minerals in soil, and therefore the determined results would not be the true “total N” for soils that contained large amount of the fixed N. The mineral fixedN averaged 166 mg kg-1, accounting for 10.1% of the total N while the residual fixed N amounted to 30.4 mg kg-1, equivalent to 1.9% of the total N or 18.3% of the total fixed N. The residual fixed N was correlated neither to organic matter nor to total N, but closely related to the total fixed N with a correlation coefficient of 0.598 (n=40), showing that the fixed N was the sole source of the residues. Soils having high residues of the fixed N were just those containing high fixed N, and soils containing high fixed N were just those containing high amount of 2:1 minerals. As a result, Kjeldahl’s method could not give a true value of the total N for such soils. However, for those containing small or little amount of 2:1 minerals, there was no significant difference in results measured by these methods.
