Vitamin D deficiency(VDD)represents a significant nutritional concern among children and adolescents.The estimated prevalence of VDD in China is 46.8%in this population^([1]).VDD during childhood and adolescence has b...Vitamin D deficiency(VDD)represents a significant nutritional concern among children and adolescents.The estimated prevalence of VDD in China is 46.8%in this population^([1]).VDD during childhood and adolescence has been associated with the onset of various conditions,including acute respiratory infections,asthma,atopic dermatitis,and food allergies^([2]).Multiple factors,including age,sun exposure,adiposity,and genetics,influence vitamin D levels^([2,3]).Increasing attention has been directed toward understanding the environmental determinants that may influence vitamin D status.Given the potential of metallic pollutants to disrupt endocrine function and their ubiquity in the environment,investigating the effects of metal exposure on human vitamin D status,particularly in vulnerable populations,is imperative.展开更多
Background:Corchorus olitorius L.(Malvaceae)is a green leafy vegetable widely consumed in the Middle East and valued for its rich nutritional content.Its essential oils,an important class of secondary metabolites,are ...Background:Corchorus olitorius L.(Malvaceae)is a green leafy vegetable widely consumed in the Middle East and valued for its rich nutritional content.Its essential oils,an important class of secondary metabolites,are of growing interest for potential use in cosmetics and fragrance industries due to their bioactive properties.Methods:Leaves of C.olitorius cultivated in Egypt were extracted using microwave-assisted hydrodistillation(MAHD)and conventional hydrodistillation(HD).The oils were analyzed by gas chromatography-mass spectrometry(GC-MS)to characterize their chemical profiles.In vitro assays were conducted to evaluate their anti-elastase and anti-collagenase activities,and in silico studies were performed to predict the pharmacokinetic and pharmacodynamic properties of major constituents.Results:GC-MS analysis showed that aldehydes,alkanes,fatty alcohols,fatty acids,and their derivatives were the predominant compound classes in both oils.Notably,oxygenated diterpenes(11.22%)were present exclusively in the MAHD oil,while triterpenoids(16.37%)were found only in the HD oil.The MAHD oil demonstrated stronger In vitro anti-elastase(IC_(50)=42.5μg/mL)and anti-collagenase(IC_(50)=131.5μg/mL)activities compared to the HD oil(IC_(50)=66.7 and 206.8μg/mL,respectively),reflecting an approximate 36.3%improvement in elastase inhibition and 36.4%improvement in collagenase inhibition.In silico docking indicated that the triterpenoidβ-amyrin acetate showed the highest predicted binding affinity for porcine pancreatic elastase(PDB ID:6QEO,ΔG=−8.1 kcal/mol)and collagenase(PDB ID:456C,ΔG=−9.1 kcal/mol)among the major compounds analyzed.Conclusion:These findings demonstrate that MAHD is a greener and more efficient extraction method,yielding oil with enhanced enzyme inhibitory activity compared to conventional HD.The promising anti-elastase and anti-collagenase properties suggest that C.olitorius MAHD oil could serve as a potential candidate for anti-aging cosmetic formulations,following further validation.展开更多
为比较超声辅助水蒸气蒸馏法与传统水蒸气蒸馏法所提白胡椒精油在挥发性成分上的差异,本研究采用上述两种方法制备精油。继而结合溶剂萃取(Liquid-Liquid Extraction,LLE),采用全二维气相色谱-嗅闻-飞行时间质谱联用技术(Comprehensive ...为比较超声辅助水蒸气蒸馏法与传统水蒸气蒸馏法所提白胡椒精油在挥发性成分上的差异,本研究采用上述两种方法制备精油。继而结合溶剂萃取(Liquid-Liquid Extraction,LLE),采用全二维气相色谱-嗅闻-飞行时间质谱联用技术(Comprehensive Two-Dimensional Gas Chromatography Olfactometry Time of Flight Mass Spectrometry,GC×GC-O-TOF-MS)对样品进行了全面解析。采用香气提取物稀释分析法(Aroma Extraction Dilution Analysis,AEDA),筛选稀释因子(Flavor Dilution,FD)≥32的香气活性化合物进行精确定量,计算其气味活性值(Odor Activity Value,OAV)。结果表明,两种白胡椒精油共鉴定出109种挥发性成分,其中有49种萜烯类,27种醇类,12种酮类,7种醛类,5种酯类和9种其它类化合物。通过对比两种白胡椒精油挥发性成分的种类和浓度,发现超声辅助水蒸气蒸馏法不仅具有较高的精油提取率,且对萜烯类化合物的提取效率优势显著。从两种白胡椒精油中嗅闻到41种香气活性化合物的气味,OAV结果发现,超声辅助提取法制得的白胡椒精油中3-蒈烯、月桂烯、α-松油烯、(+)-柠檬烯、对伞花烃、E-石竹烯、芳樟醇、4-萜烯醇等重要的香气活性化合物的OAV均得到了提升,可见超声辅助在提高胡椒精油提取率的同时,还有效促进了胡椒精油中香气活性化合物的释放,研究为改善水蒸气蒸馏提取植物精油的风味品质提供了一种方法和思路。展开更多
基金supported by grants from the National Natural Science Foundation of China(G.F.Wang,grant number 82204071)(P.Y.Su,grant numbers 81874268 and 82473655)the Research Funds of the Center for Big Data and Population Health of IHM(P.Y.Su,No.JKS2023016)Anhui Provincial Health Commission Scientific Research Project(Y.Zhou,No.AHWJ2023A30027)。
文摘Vitamin D deficiency(VDD)represents a significant nutritional concern among children and adolescents.The estimated prevalence of VDD in China is 46.8%in this population^([1]).VDD during childhood and adolescence has been associated with the onset of various conditions,including acute respiratory infections,asthma,atopic dermatitis,and food allergies^([2]).Multiple factors,including age,sun exposure,adiposity,and genetics,influence vitamin D levels^([2,3]).Increasing attention has been directed toward understanding the environmental determinants that may influence vitamin D status.Given the potential of metallic pollutants to disrupt endocrine function and their ubiquity in the environment,investigating the effects of metal exposure on human vitamin D status,particularly in vulnerable populations,is imperative.
基金Egyptian Science and Technology and Innovation Fund(STIFA)for the support of the study through grant No.46667 entitled“Sustainability of Lab Capacities of the Center of Drug Discovery Research and Development”.
文摘Background:Corchorus olitorius L.(Malvaceae)is a green leafy vegetable widely consumed in the Middle East and valued for its rich nutritional content.Its essential oils,an important class of secondary metabolites,are of growing interest for potential use in cosmetics and fragrance industries due to their bioactive properties.Methods:Leaves of C.olitorius cultivated in Egypt were extracted using microwave-assisted hydrodistillation(MAHD)and conventional hydrodistillation(HD).The oils were analyzed by gas chromatography-mass spectrometry(GC-MS)to characterize their chemical profiles.In vitro assays were conducted to evaluate their anti-elastase and anti-collagenase activities,and in silico studies were performed to predict the pharmacokinetic and pharmacodynamic properties of major constituents.Results:GC-MS analysis showed that aldehydes,alkanes,fatty alcohols,fatty acids,and their derivatives were the predominant compound classes in both oils.Notably,oxygenated diterpenes(11.22%)were present exclusively in the MAHD oil,while triterpenoids(16.37%)were found only in the HD oil.The MAHD oil demonstrated stronger In vitro anti-elastase(IC_(50)=42.5μg/mL)and anti-collagenase(IC_(50)=131.5μg/mL)activities compared to the HD oil(IC_(50)=66.7 and 206.8μg/mL,respectively),reflecting an approximate 36.3%improvement in elastase inhibition and 36.4%improvement in collagenase inhibition.In silico docking indicated that the triterpenoidβ-amyrin acetate showed the highest predicted binding affinity for porcine pancreatic elastase(PDB ID:6QEO,ΔG=−8.1 kcal/mol)and collagenase(PDB ID:456C,ΔG=−9.1 kcal/mol)among the major compounds analyzed.Conclusion:These findings demonstrate that MAHD is a greener and more efficient extraction method,yielding oil with enhanced enzyme inhibitory activity compared to conventional HD.The promising anti-elastase and anti-collagenase properties suggest that C.olitorius MAHD oil could serve as a potential candidate for anti-aging cosmetic formulations,following further validation.
文摘为比较超声辅助水蒸气蒸馏法与传统水蒸气蒸馏法所提白胡椒精油在挥发性成分上的差异,本研究采用上述两种方法制备精油。继而结合溶剂萃取(Liquid-Liquid Extraction,LLE),采用全二维气相色谱-嗅闻-飞行时间质谱联用技术(Comprehensive Two-Dimensional Gas Chromatography Olfactometry Time of Flight Mass Spectrometry,GC×GC-O-TOF-MS)对样品进行了全面解析。采用香气提取物稀释分析法(Aroma Extraction Dilution Analysis,AEDA),筛选稀释因子(Flavor Dilution,FD)≥32的香气活性化合物进行精确定量,计算其气味活性值(Odor Activity Value,OAV)。结果表明,两种白胡椒精油共鉴定出109种挥发性成分,其中有49种萜烯类,27种醇类,12种酮类,7种醛类,5种酯类和9种其它类化合物。通过对比两种白胡椒精油挥发性成分的种类和浓度,发现超声辅助水蒸气蒸馏法不仅具有较高的精油提取率,且对萜烯类化合物的提取效率优势显著。从两种白胡椒精油中嗅闻到41种香气活性化合物的气味,OAV结果发现,超声辅助提取法制得的白胡椒精油中3-蒈烯、月桂烯、α-松油烯、(+)-柠檬烯、对伞花烃、E-石竹烯、芳樟醇、4-萜烯醇等重要的香气活性化合物的OAV均得到了提升,可见超声辅助在提高胡椒精油提取率的同时,还有效促进了胡椒精油中香气活性化合物的释放,研究为改善水蒸气蒸馏提取植物精油的风味品质提供了一种方法和思路。
