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Graph Guide Diffusion Solvers with Noises for Travelling Salesman Problem
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作者 Yan Kong Xinpeng Guo Chih-Hsien Hsia 《Computers, Materials & Continua》 2026年第3期689-707,共19页
With the development of technology,diffusion model-based solvers have shown significant promise in solving Combinatorial Optimization(CO)problems,particularly in tackling Non-deterministic Polynomial-time hard(NP-hard... With the development of technology,diffusion model-based solvers have shown significant promise in solving Combinatorial Optimization(CO)problems,particularly in tackling Non-deterministic Polynomial-time hard(NP-hard)problems such as the Traveling Salesman Problem(TSP).However,existing diffusion model-based solvers typically employ a fixed,uniform noise schedule(e.g.,linear or cosine annealing)across all training instances,failing to fully account for the unique characteristics of each problem instance.To address this challenge,we present GraphGuided Diffusion Solvers(GGDS),an enhanced method for improving graph-based diffusion models.GGDS leverages Graph Neural Networks(GNNs)to capture graph structural information embedded in node coordinates and adjacency matrices,dynamically adjusting the noise levels in the diffusion model.This study investigates the TSP by examining two distinct time-step noise generation strategies:cosine annealing and a Neural Network(NN)-based approach.We evaluate their performance across different problem scales,particularly after integrating graph structural information.Experimental results indicate that GGDS outperforms previous methods with average performance improvements of 18.7%,6.3%,and 88.7%on TSP-500,TSP-100,and TSP-50,respectively.Specifically,GGDS demonstrates superior performance on TSP-500 and TSP-50,while its performance on TSP-100 is either comparable to or slightly better than that of previous methods,depending on the chosen noise schedule and decoding strategy. 展开更多
关键词 Combinatorial optimization problem diffusion model noise schedule traveling salesman problem
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Mindfulness and internalizing/externalizing problems among adolescents:Ethnicity moderation and psychological capital mediation
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作者 Jia Wu Wei Luo +4 位作者 Qianguo Xiao Qinhong Xie Xiaodong Li Taiyong Bi Hui Kou 《Journal of Psychology in Africa》 2026年第1期97-106,共10页
This study examines the mediating role of positive psychological capital and the moderating role of ethnicity in the relationship between mindfulness and internalizing/externalizing problems among adolescents.The stud... This study examines the mediating role of positive psychological capital and the moderating role of ethnicity in the relationship between mindfulness and internalizing/externalizing problems among adolescents.The study sample comprized Chinese adolescents(N=637 ethnic minority;females=40.97%,meam age=12.68,SD=0.49 years;N=636 Han;females=49.06%,mean age=12.71,SD=0.47 years).The participants completed the Child and Adolescent Mindfulness Measure,the Positive Psycap Questionnaire,and the Youth Self-Report.Results from the moderated mediation analysis showed mindfulness was negatively associated with both internalizing and externalizing problems.Ethnicity moderated the relationship between mindfulness and internalizing problems to be stronger for Han adolescents compared to ethnic minority adolescents.Psychological capital mediated the relationship between mindfulness and internalizing problems in both groups,with a negative direction.Findings support the Conservation of Resources theory and highlight mindfulness as a personal resource fostering adolescent well-being in multicultural contexts. 展开更多
关键词 Ethnic minority adolescent MINDFULNESS positive psychological capital internalizing problems externalizing problems
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CDH6调控胃腺癌细胞MGC-803自噬进程的机制研究
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作者 陆东兰 吕宇锋 +2 位作者 惠明旭 秦恒 白雪 《中国处方药》 2026年第3期102-105,共4页
目的 本研究以胃腺癌细胞MGC-803为实验对象,通过探索钙黏蛋白6(CDH6)潜在的生物学功能,尝试揭示CDH6在调控胃腺癌细胞自噬过程中的分子机制,并探讨其对磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号轴(PI3K/Akt/mTOR)及细胞... 目的 本研究以胃腺癌细胞MGC-803为实验对象,通过探索钙黏蛋白6(CDH6)潜在的生物学功能,尝试揭示CDH6在调控胃腺癌细胞自噬过程中的分子机制,并探讨其对磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号轴(PI3K/Akt/mTOR)及细胞生物学行为的影响。方法 从癌症基因组图谱数据库(TCGA)中获取375例胃腺癌组织和32例癌旁组织转录组数据。根据CDH6表达情况分为对照组、空载体对照组、CDH6过表达组、CDH6敲低组。采用蛋白印迹(Western blot)法检测CDH6及自噬相关蛋白[微管相关蛋白1轻链3(LC3)、泛素结合蛋白p62(p62)]及PI3K/Akt/mTOR通路关键磷酸化蛋白表达,采用细胞计数试剂盒-8(CCK-8)法评估细胞增殖活力,透射电子显微镜观察自噬体数量变化。结果 TCGA数据显示,胃腺癌组织中CDH6 mRNA表达显著高于癌旁组织(P<0.05);与空载体对照组相比,CDH6过表达组24 h、48 h、72 h的吸光度(OD)值显著升高,自噬体数量显著降低[(3.4±0.6)个/细胞vs.(1.1±0.3)个/细胞](P<0.05),CDH6敲低组24 h、48 h、72 h的OD值显著降低,自噬体数量显著升高[(3.4±0.6)个/细胞vs.(5.8±0.7)个/细胞](P<0.05);与空载体对照组比较,CDH6过表达组的LC3-II/I水平明显降低,p62水平明显升高(P<0.05),CDH6敲低组LC3-II/I水平显著升高,p62水平显著降低(P<0.05);与空载体对照组比较,CDH6过表达组磷酸化磷脂酰肌醇3-激酶(p-PI3K)、磷酸化蛋白激酶B(p-Akt)和磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)表达增加(P<0.05),CDH6敲低组p-PI3K和p-Akt及p-mTOR的表达降低(P<0.05)。结论 CDH6在胃腺癌中呈高表达,其可通过激活PI3K/Akt/mTOR信号通路抑制细胞自噬,从而促进胃腺癌细胞的增殖,有望成为自噬相关的潜在分子标志物及治疗干预靶点。 展开更多
关键词 cdh6 胃腺癌 自噬 PI3K/Akt/mTOR通路 细胞增殖
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Solutions of three-body problem based on an equivalent system approach
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作者 Jing Tang Xing 《Acta Mechanica Sinica》 2026年第1期292-309,共18页
Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fund... Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fundamental bases of this paper,are revealed.Based on these findings,an equivalent system is developed,which is a 2-body system with its total mass,constant angular momentum,kinetic and potential energies same as the total ones of three relative motions,so that it can be solved using the well-known theory of the 2-body system.From the solution of an equivalent system with the revealed characteristics of three relative motions,the general theoretical solutions of the 3-body system are obtained in the curve-integration forms along the orbits in the imaged radial motion space.The possible periodical orbits with generalised Kepler’s law are presented.Following the description and mathematical demonstrations of the proposed methods,the examples including Euler’s/Lagrange’s problems,and a reported numerical one are solved to validate the proposed methods.The methods derived from the 3-body system are extended to N-body problems. 展开更多
关键词 Three-body problem Equivalent system with solutions Orbit-equation of a conic section Generalised reduced mass Chaotic motions Generalised Kepler’s law N-body problem
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Collaborative scheduling problem pertaining to launch and recovery operations for carrier aircraft
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作者 GUO Fang HAN Wei +3 位作者 LIU Yujie SU Xichao LIU Jie LI Changjiu 《Journal of Systems Engineering and Electronics》 2026年第1期287-306,共20页
The proliferation of carrier aircraft and the integration of unmanned aerial vehicles(UAVs)on aircraft carriers present new challenges to the automation of launch and recovery operations.This paper investigates a coll... The proliferation of carrier aircraft and the integration of unmanned aerial vehicles(UAVs)on aircraft carriers present new challenges to the automation of launch and recovery operations.This paper investigates a collaborative scheduling problem inherent to the operational processes of carrier aircraft,where launch and recovery tasks are conducted concurrently on the flight deck.The objective is to minimize the cumulative weighted waiting time in the air for recovering aircraft and the cumulative weighted delay time for launching aircraft.To tackle this challenge,a multiple population self-adaptive differential evolution(MPSADE)algorithm is proposed.This method features a self-adaptive parameter updating mechanism that is contingent upon population diversity,an asynchronous updating scheme,an individual migration operator,and a global crossover mechanism.Additionally,comprehensive experiments are conducted to validate the effectiveness of the proposed model and algorithm.Ultimately,a comparative analysis with existing operation modes confirms the enhanced efficiency of the collaborative operation mode. 展开更多
关键词 carrier aircraft collaborative scheduling problem LAUNCH RECOVERY multiple population differential evolution
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Centralized Circumcentered-Reflection Method for Solving the Convex Feasibility Problem in Sparse Signal Recovery
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作者 Chunmei LI Bangjun CHEN Xuefeng DUAN 《Journal of Mathematical Research with Applications》 2026年第1期119-133,共15页
Convex feasibility problems are widely used in image reconstruction, sparse signal recovery, and other areas. This paper is devoted to considering a class of convex feasibility problem arising from sparse signal recov... Convex feasibility problems are widely used in image reconstruction, sparse signal recovery, and other areas. This paper is devoted to considering a class of convex feasibility problem arising from sparse signal recovery. We first derive the projection formulas for a vector onto the feasible sets. The centralized circumcentered-reflection method is designed to solve the convex feasibility problem. Some numerical experiments demonstrate the feasibility and effectiveness of the proposed algorithm, showing superior performance compared to conventional alternating projection methods. 展开更多
关键词 convex feasibility problem centralized circumcentered-re ection method sparse signal recovery compressed sensing
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Quality-guaranteed Dubins Path Planning for USV Based on Mixed-integer Piecewise linear Programming for Addressing the Extended Minimum-time Intercept Problem
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作者 Xing Zhou Kelin Zhu +3 位作者 Shuang Liu Zhaoqing Li Wenxin Zhang Kang Du 《哈尔滨工程大学学报(英文版)》 2026年第1期216-227,共12页
During the use of robotics in applications such as antiterrorism or combat,a motion-constrained pursuer vehicle,such as a Dubins unmanned surface vehicle(USV),must get close enough(within a prescribed zero or positive... During the use of robotics in applications such as antiterrorism or combat,a motion-constrained pursuer vehicle,such as a Dubins unmanned surface vehicle(USV),must get close enough(within a prescribed zero or positive distance)to a moving target as quickly as possible,resulting in the extended minimum-time intercept problem(EMTIP).Existing research has primarily focused on the zero-distance intercept problem,MTIP,establishing the necessary or sufficient conditions for MTIP optimality,and utilizing analytic algorithms,such as root-finding algorithms,to calculate the optimal solutions.However,these approaches depend heavily on the properties of the analytic algorithm,making them inapplicable when problem settings change,such as in the case of a positive effective range or complicated target motions outside uniform rectilinear motion.In this study,an approach employing a high-accuracy and quality-guaranteed mixed-integer piecewise-linear program(QG-PWL)is proposed for the EMTIP.This program can accommodate different effective interception ranges and complicated target motions(variable velocity or complicated trajectories).The high accuracy and quality guarantees of QG-PWL originate from elegant strategies such as piecewise linearization and other developed operation strategies.The approximate error in the intercept path length is proved to be bounded to h^(2)/(4√2),where h is the piecewise length. 展开更多
关键词 Minimum-time intercept problem Dubins vehicle Mixed-integer piecewise-linear program LINEARIZATION Approximate error trigonometric function USV
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Cooperative Metaheuristics with Dynamic Dimension Reduction for High-Dimensional Optimization Problems
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作者 Junxiang Li Zhipeng Dong +2 位作者 Ben Han Jianqiao Chen Xinxin Zhang 《Computers, Materials & Continua》 2026年第1期1484-1502,共19页
Owing to their global search capabilities and gradient-free operation,metaheuristic algorithms are widely applied to a wide range of optimization problems.However,their computational demands become prohibitive when ta... Owing to their global search capabilities and gradient-free operation,metaheuristic algorithms are widely applied to a wide range of optimization problems.However,their computational demands become prohibitive when tackling high-dimensional optimization challenges.To effectively address these challenges,this study introduces cooperative metaheuristics integrating dynamic dimension reduction(DR).Building upon particle swarm optimization(PSO)and differential evolution(DE),the proposed cooperative methods C-PSO and C-DE are developed.In the proposed methods,the modified principal components analysis(PCA)is utilized to reduce the dimension of design variables,thereby decreasing computational costs.The dynamic DR strategy implements periodic execution of modified PCA after a fixed number of iterations,resulting in the important dimensions being dynamically identified.Compared with the static one,the dynamic DR strategy can achieve precise identification of important dimensions,thereby enabling accelerated convergence toward optimal solutions.Furthermore,the influence of cumulative contribution rate thresholds on optimization problems with different dimensions is investigated.Metaheuristic algorithms(PSO,DE)and cooperative metaheuristics(C-PSO,C-DE)are examined by 15 benchmark functions and two engineering design problems(speed reducer and composite pressure vessel).Comparative results demonstrate that the cooperative methods achieve significantly superior performance compared to standard methods in both solution accuracy and computational efficiency.Compared to standard metaheuristic algorithms,cooperative metaheuristics achieve a reduction in computational cost of at least 40%.The cooperative metaheuristics can be effectively used to tackle both high-dimensional unconstrained and constrained optimization problems. 展开更多
关键词 Dimension reduction modified principal components analysis high-dimensional optimization problems cooperative metaheuristics metaheuristic algorithms
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Non-Newtonian rivulet flows on an inclined planar surface applying the 2nd Stokes problem
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作者 S.V.ERSHKOV E.S.BARANOVSKII A.V.YUDIN 《Applied Mathematics and Mechanics(English Edition)》 2026年第1期153-164,共12页
The newly formulated non-Newtonian rivulet flows streaming down an inclined planar surface,with additional periodic perturbations arising from the application of the 2nd Stokes problem to the investigation of rivulet ... The newly formulated non-Newtonian rivulet flows streaming down an inclined planar surface,with additional periodic perturbations arising from the application of the 2nd Stokes problem to the investigation of rivulet dynamics,are demonstrated in the current research.Hereby,the 2nd Stokes problem assumes that the surface,with a thin shared layer of the fluid on it,oscillates in a harmonic manner along the x-axis of the rivulet flow,which coincides with the main flow direction streaming down the underlying surface.We obtain the exact extension of the rivulet flow family,clarifying the structure of the pressure field,which fully absorbs the arising perturbation.The profile of the velocity field is assumed to be Gaussian-type with a non-zero level of plasticity.Hence,the absolutely non-Newtonian case of the viscoplastic flow solution,which satisfies the motion and continuity equations,is considered(with particular cases of exact solutions for pressure).The perturbed governing equations of motion for rivulet flows then result in the Riccati-type ordinary differential equation(ODE),describing the dynamics of the coordinate x(t).The approximated schematic dynamics are presented in graphical plots. 展开更多
关键词 rivulet flow non-Newtonian fluid creeping viscoplastic flow 1st/2nd Stokes problem
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AUGMENTED SUBSPACE SCHEME FOR EIGENVALUE PROBLEM BY WEAK GALERKIN FINITE ELEMENT METHOD
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作者 Yue Feng Zhijin Guan +1 位作者 Hehu Xie Chenguang Zhou, 《Journal of Computational Mathematics》 2026年第1期135-164,共30页
This study proposes a class of augmented subspace schemes for the weak Galerkin(WG)finite element method used to solve eigenvalue problems.The augmented subspace is built with the conforming linear finite element spac... This study proposes a class of augmented subspace schemes for the weak Galerkin(WG)finite element method used to solve eigenvalue problems.The augmented subspace is built with the conforming linear finite element space defined on the coarse mesh and the eigen-function approximations in the WG finite element space defined on the fine mesh.Based on this augmented subspace,solving the eigenvalue problem in the fine WG finite element space can be reduced to the solution of the linear boundary value problem in the same WG finite element space and a low dimensional eigenvalue problem in the augmented sub-space.The proposed augmented subspace techniques have the second order convergence rate with respect to the coarse mesh size,as demonstrated by the accompanying error esti-mates.Finally,a few numerical examples are provided to validate the proposed numerical techniques. 展开更多
关键词 Eigenvalue problem Augmented subspace scheme Weak Galerkin finite ele-ment method Second order convergence rate
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Neural boundary shape functions in physics-informed neural networks for discontinuous and high-frequency problems
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作者 P.T.NGUYEN K.A.LUONG J.H.LEE 《Applied Mathematics and Mechanics(English Edition)》 2026年第2期423-442,共20页
Physics-informed neural networks(PINNs)have been shown as powerful tools for solving partial differential equations(PDEs)by embedding physical laws into the network training.Despite their remarkable results,complicate... Physics-informed neural networks(PINNs)have been shown as powerful tools for solving partial differential equations(PDEs)by embedding physical laws into the network training.Despite their remarkable results,complicated problems such as irregular boundary conditions(BCs)and discontinuous or high-frequency behaviors remain persistent challenges for PINNs.For these reasons,we propose a novel two-phase framework,where a neural network is first trained to represent shape functions that can capture the irregularity of BCs in the first phase,and then these neural network-based shape functions are used to construct boundary shape functions(BSFs)that exactly satisfy both essential and natural BCs in PINNs in the second phase.This scheme is integrated into both the strong-form and energy PINN approaches,thereby improving the quality of solution prediction in the cases of irregular BCs.In addition,this study examines the benefits and limitations of these approaches in handling discontinuous and high-frequency problems.Overall,our method offers a unified and flexible solution framework that addresses key limitations of existing PINN methods with higher accuracy and stability for general PDE problems in solid mechanics. 展开更多
关键词 physics-informed neural network(PINN) boundary shape function(BSF) strong-form approach energy approach DISCONTINUITY high-frequency problem
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一个CDH23基因新型复合杂合突变导致的耳聋家系分析 被引量:1
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作者 孙毅 葛雨彤 +2 位作者 刘雅琳 韩庆亚 郑宏 《听力学及言语疾病杂志》 北大核心 2025年第1期45-49,共5页
目的 研究一个家系中2例非综合征型遗传性聋患者的致病原因。方法 收集该耳聋家系临床检查结果资料,采集静脉血后提取DNA,应用全外显子组测序技术分析可疑致病基因,并通过Sanger测序对变异进行验证。结果 该家系中2代5人,先证者(Ⅱ-2,9... 目的 研究一个家系中2例非综合征型遗传性聋患者的致病原因。方法 收集该耳聋家系临床检查结果资料,采集静脉血后提取DNA,应用全外显子组测序技术分析可疑致病基因,并通过Sanger测序对变异进行验证。结果 该家系中2代5人,先证者(Ⅱ-2,9岁)和其弟弟(Ⅱ-3)均为迟发性感音神经性听力损失,患者父母听力正常。先证者弟弟(Ⅱ-3)携带相同突变位点。基因检测结果显示,先证者携带CDH23基因c.4762C>T(p.ARG1588TRP)和c.6604G>A(p.ASP2202ASN)。根据美国医学遗传学与基因组学学会(American College of Medical Genetics and Genomics, ACMG)遗传变异分类标准与指南分析显示,c.4762C>T和c.6604G>A位点均为致病突变。蛋白功能分析发现,变异后氨基酸改变造成蛋白结构发生改变,进而影响蛋白质功能。结论 CDH23基因c.4762C>T和c.6604G>A复合杂合突变位点组合很可能是该家系耳聋致病基因。 展开更多
关键词 cdh23基因 遗传性聋 基因测序 突变检测
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基于H3K18乳酸化修饰介导CDH1表达研究左归降糖解郁方改善糖尿病并发抑郁症海马星形胶质细胞乳酸生成的作用
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作者 熊耀 李薇 +6 位作者 雷诗卉 王瑾茜 刘检 蔺晓源 易健 王宇红 杨蕙 《中国中药杂志》 北大核心 2025年第22期6410-6418,共9页
研究左归降糖解郁方调控糖尿病并发抑郁症大鼠海马组蛋白3的第18位赖氨酸(H3K18)乳酸化(H3K18la)修饰并改善星形胶质细胞乳酸生成的机制。SD大鼠按随机数字表法分为正常组,模型组,左归降糖解郁方高、中、低剂量组,灌胃给药28 d后,血糖... 研究左归降糖解郁方调控糖尿病并发抑郁症大鼠海马组蛋白3的第18位赖氨酸(H3K18)乳酸化(H3K18la)修饰并改善星形胶质细胞乳酸生成的机制。SD大鼠按随机数字表法分为正常组,模型组,左归降糖解郁方高、中、低剂量组,灌胃给药28 d后,血糖试纸检测空腹血糖,采用ELISA法检测胰岛素并计算胰岛素抵抗指数,采用强迫游泳实验和开野实验评价动物抑郁样行为,采用RT-PCR检测钙黏蛋白1(CDH1)mRNA表达。随后将SD大鼠随机分为正常组、模型组、假手术组、L-乳酸组、左归降糖解郁方组、2-脱氧-D-葡萄糖(2-DG)组,干预结束后检测动物抑郁样行为,采用胶质纤维酸性蛋白(GFAP)/5-溴-2'-脱氧尿苷(Brdu)双重免疫荧光评估星形胶质细胞增殖水平,采用ELISA检测海马乳酸含量,采用Western blot检测细胞周期蛋白B1(cyclin B1)、H3K18la、CDH1、6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶3(PFKFB3)表达。结果表明,与正常组比较,模型组大鼠空腹血糖、胰岛素以及胰岛素抵抗水平显著升高(P<0.01),动物在强迫游泳实验中的不动时间显著延长而在开野实验中的活动次数显著减少(P<0.01)。与模型组比较,左归降糖解郁方高、中剂量可显著降低模型大鼠空腹血糖、胰岛素以及胰岛素抵抗水平(P<0.01),减少动物不动时间并增加活动次数(P<0.01)。RT-PCR实验发现,与模型组比较,左归降糖解郁方可显著降低CDH1 mRNA表达(P<0.05)。与模型组比较,左归降糖解郁方可显著上调大鼠海马乳酸含量(P<0.05),促进星形胶质细胞增殖(P<0.01),增加cyclin B1、H3K18la、PFKFB3蛋白表达(P<0.05或P<0.01),减少CDH1蛋白表达(P<0.01);2-DG可逆转左归降糖解郁方的上述作用。与假手术组比较,侧脑室注射L-乳酸可以增加海马乳酸含量及H3K18la表达(P<0.05或P<0.01),促进星形胶质细胞增殖(P<0.01),增加海马cyclin B1和PFKFB3表达而降低CDH1表达(P<0.05或P<0.01)。因此,左归降糖解郁方可增加糖尿病并发抑郁症大鼠海马PFKFB3表达并促进乳酸生成,其机制可能与增加海马H3K18la修饰并抑制CDH1表达,进而促进星形胶质细胞增殖有关。 展开更多
关键词 左归降糖解郁方 糖尿病并发抑郁症 星形胶质细胞 组蛋白3的第18位赖氨酸乳酸化(H3K18la) 钙黏蛋白1(cdh1)
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基于氟化聚乙烯亚胺构建高效递送载体实现Cdh 23全长质粒在HEI-OC1细胞中的转染
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作者 李冰倩 李木兰 +3 位作者 夏淼 刘真 王兰 马朋 《中国生物化学与分子生物学报》 北大核心 2025年第9期1349-1359,共11页
CDH 23基因是Usher综合征中USH1D亚型的致病突变基因,本研究构建2种不同启动子的野生型Cdh 23全长质粒(~16 kb),利用氟化聚乙烯亚胺(fluorinated-polyethyleneimine,FPEI)作为递送载体对小鼠听觉细胞系(house ear institute-organ of co... CDH 23基因是Usher综合征中USH1D亚型的致病突变基因,本研究构建2种不同启动子的野生型Cdh 23全长质粒(~16 kb),利用氟化聚乙烯亚胺(fluorinated-polyethyleneimine,FPEI)作为递送载体对小鼠听觉细胞系(house ear institute-organ of corti 1,HEI-OC1)进行转染,通过评价体外转染效果,获得最佳表达质粒。首先,利用傅里叶变换红外吸收光谱对FPEI的合成结果进行分析,证明FPEI的成功合成。之后,通过琼脂糖凝胶阻滞实验、Zeta电位实验、动态光散射实验分别对FPEI的质粒包裹能力、形成复合物的表面电位和水合直径进行表征,证明FPEI具有良好的质粒包裹能力,在高质量比时FPEI质粒复合物均带正电,粒径分布在100~300 nm之间。通过Cell Counting Kit-8(CCK-8)和流式细胞术验证了FPEI在HEI-OC1细胞中的低细胞毒性和高转染效率。同时将FPEI与Lipofectamine 3000及不同质量的PEI(25K、40K)转染试剂进行比较,发现FPEI的转染效率显著优于上述试剂。实时定量聚合酶链式反应(quantitative real-time polymerase chain reaction,qRT-PCR)及Western印迹结果表明,CAG启动子效果优于CMV启动子,可作为最佳表达质粒进行后续体内研究。此外,细胞免疫荧光染色证实过表达的CDH23主要分布于细胞质中。以上结果证明,FPEI可作为以Cdh 23为代表的大基因体外过表达的高效递送载体,这为后续USH1D综合征体内基因治疗提供了重要的实验依据。 展开更多
关键词 氟化聚乙烯亚胺 cdh 23基因 大基因过表达 HEI-OC1细胞 USH1D综合征
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The Orlicz Minkowski Problem for Logarithmic Capacity 被引量:1
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作者 HE Min LIU Lijuan ZENG Hui 《Wuhan University Journal of Natural Sciences》 2025年第5期471-478,共8页
The Orlicz Minkowski problem for logarithmic capacity seeks to determine the necessary and sufficient conditions for a given finite Borel measure,such that it is the Orlicz logarithmic capacitary measure of a convex b... The Orlicz Minkowski problem for logarithmic capacity seeks to determine the necessary and sufficient conditions for a given finite Borel measure,such that it is the Orlicz logarithmic capacitary measure of a convex body.The Orlicz Minkowski problem for loga-rithmic capacity includes the Minkowski problem for logarithmic capacity and the Lp Minkowski problem for logarithmic capacity as special cases.The discrete case has been solved by the researchers.In this paper,we solve the Orlicz Minkowski problem for logarithmic capacity with respect to general Borel measures by applying an approximation scheme. 展开更多
关键词 Orlicz Minkowski problem capacity convex body
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Pilot Study of a School-Based Parenting Intervention:Prevention of Emotional and Behavioral Problems among Chinese Children 被引量:1
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作者 Jin Peng Jiabei He Na Li 《International Journal of Mental Health Promotion》 2025年第7期953-977,共25页
Background:Parenting exerts a profound influence on children’s mental health and behavioral development.Despite the high prevalence of children’s emotional and behavioral problems(CEBP)in China,evidence-based parent... Background:Parenting exerts a profound influence on children’s mental health and behavioral development.Despite the high prevalence of children’s emotional and behavioral problems(CEBP)in China,evidence-based parenting interventions remain scarcely investigated as preventive public health strategies.This pilot study evaluated a school-based intervention for preventing CEBP.Methods:We employed a quasi-experimental design with propensity score matching(PSM)to select 28 families(intervention:n=13;control:n=15)from two matched urban primary schools.Quantitative data from seven validated scales were analyzed using t-tests and ANCOVA.Qualitative insights were derived from 10 semi-structured interviews via thematic analysis.Results:Compared to the control group,the intervention group demonstrated significantly greater improvements in CEBP(p=0.020,Cohen’s d=0.92),parental adjustment(p=0.031,Cohen’s d=0.80),parenting confidence(p=0.003,Cohen’s d=1.04),and parentchild relationships(p=0.001,Cohen’s d=1.46).Non-significant effects were observed for parenting style,parental relationship,and parenting conflict(p>0.05).Qualitative analysis corroborated these findings and further identified contributing factors for non-significant outcomes,including challengeswithmeasurement adaptability and inconsistent co-parenting practices.Conclusions:This pilot study suggests that an authoritative parenting style may be effective and culturally adaptable in China.Positive parenting interventions appear to mitigate CEBP by reducing risk factors and enhancing protective factors.However,improving parental relationships and parenting conflict may require targeted strategies.Given the pilot nature of this PSM-matched study(n=28),the findings should be interpreted as exploratory and used primarily for intervention refinement. 展开更多
关键词 Parenting intervention CHILDREN SCHOOL-BASED China emotional and behavioral problems
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CDH11基因过表达载体构建及其对牛成肌细胞增殖和分化的作用
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作者 刘占鑫 白彦斌 +11 位作者 牛艳梅 贾雪 李亮 蔡思怡 王轶程 张军霞 刘旭辉 张晓兰 石斌刚 胡江 赵志东 应成玉 《西北农业学报》 北大核心 2025年第12期2175-2183,共9页
通过在牛成肌细胞中过表达钙粘蛋白11(Cadherin-11,CDH11)基因并利用EDU、CCK-8、免疫荧光和实时荧光定量PCR(qRT-PCR)等技术,探讨CDH11基因对牛成肌细胞增殖与分化的作用。结果表明,过表达CDH11基因显著提高牛成肌细胞的EDU阳性率及450... 通过在牛成肌细胞中过表达钙粘蛋白11(Cadherin-11,CDH11)基因并利用EDU、CCK-8、免疫荧光和实时荧光定量PCR(qRT-PCR)等技术,探讨CDH11基因对牛成肌细胞增殖与分化的作用。结果表明,过表达CDH11基因显著提高牛成肌细胞的EDU阳性率及450 nm波长下的吸光度(P<0.05),显著增强周期蛋白依懒性激酶2(Cyclin-Dependent Kinases,CDK2)和增殖细胞核抗原(Proliferating Cell Nuclear Antigen,PCNA)的表达水平(P<0.05)。在成肌细胞分化阶段,过表达CDH11基因显著减少了肌管的面积,显著抑制成肌分化相关基因成肌分化因子(Myogenic Diffeerentiation,MYOD)、肌细胞生成素(Myogenin,MYOG)和生肌调节因子5(Myogenic Regulatory Factors5,MYF5)的表达(P<0.05)。研究表明过表达CDH11基因能够促进牛成肌细胞的增殖,但对其分化具有抑制作用。 展开更多
关键词 牛成肌细胞 钙粘蛋白11(cdh11) 增殖 分化
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槐山羊CD3D和CDH26基因遗传多态性及其与产羔数的关联分析
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作者 韩浩园 蒯子茹 +7 位作者 姚志云 李君 施会彬 权凯 赵金艳 李德立 王拥庆 尹慧茹 《中国畜牧杂志》 北大核心 2025年第11期171-178,共8页
为了探究CD3D和CDH26基因对槐山羊产羔数的影响,本研究参照NCBI数据库公布的山羊CD3D和CDH26基因全序列,利用Primer Premier 5.0软件设计引物,以122只槐山羊为研究对象,利用Sanger测序技术进行测序,应用SeqMan软件分析测序结果,对SNP位... 为了探究CD3D和CDH26基因对槐山羊产羔数的影响,本研究参照NCBI数据库公布的山羊CD3D和CDH26基因全序列,利用Primer Premier 5.0软件设计引物,以122只槐山羊为研究对象,利用Sanger测序技术进行测序,应用SeqMan软件分析测序结果,对SNP位点进行初步筛选;运用SHEsis在线软件进行SNP位点之间的连锁不平衡和单倍型分析;运用SPSS 26.0软件进行SNP位点基因型、单倍型与产羔数的关联性分析。结果表明,在CD3D基因非编码区发现7个SNP位点,分别为g.53316033 G>C、g.53316268A>G、g.53316691 G>A、g.53316752 T>C、g.53316809 C>T、g.53317246 C>T和g.53317426 C>T。在CDH26基因中检测到2个多态位点,分别为g.55861588 G>A与g.55861587 A>G,为同义突变。连锁不平衡分析发现,g.53316033 G>C与g.53316268 A>G、g.53316752 T>C与g.53316809 C>T、g.55861588 G>A与g.55861587 A>G位点间属于强连锁平衡状态(r2>0.33,D'>0.8)。单倍型分析发现CD3D基因共有21种单倍型,单倍型与槐山羊群体产羔数的关联分析发现GAACTCC单倍型个体的产羔数高于单倍型CGACTCC、CGGCTTC、GAATCCC、GAGCTCT个体产羔数(P<0.05),表明CD3D基因单倍型GAACTCC与槐山羊产羔数显著相关。在CDH26基因中发现的2个SNP不同基因型的山羊产羔数差异显著,CC基因型个体产羔数显著高于CT基因型个体的产羔数。本研究发现CD3D基因7个SNP位点的GAACTCC单倍型与CDH26基因2个SNP位点可作为槐山羊多羔性状标记辅助选择的分子标记,为槐山羊繁殖力的育种提供了一定的理论依据。 展开更多
关键词 槐山羊 CD3D基因 cdh26基因 产羔数 关联分析
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THECHORD GAUSS CURVATURE FLOW AND ITS L_(p)CHORDMINKOWSKI PROBLEM 被引量:1
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作者 Jinrong HU Yong HUANG +1 位作者 Jian LU Sinan WANG 《Acta Mathematica Scientia》 2025年第1期161-179,共19页
In this paper,the L_(p)chord Minkowski problem is concerned.Based on the results shown in[20],we obtain a new existence result of solutions to this problem in terms of smooth measures by using a nonlocal Gauss curvatu... In this paper,the L_(p)chord Minkowski problem is concerned.Based on the results shown in[20],we obtain a new existence result of solutions to this problem in terms of smooth measures by using a nonlocal Gauss curvature flow for p>−n with p≠0. 展开更多
关键词 L_(p)chord Minkowski problem new Monge-Ampere equation geometric fow
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On Waring-Goldbach Problem for Two Squares, Two Cubes and Two Fifth Powers
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作者 LI Jinjiang ZHAO Chenyang +1 位作者 LIU Zishun ZHANG Min 《数学进展》 北大核心 2025年第4期735-748,共14页
Let Pr denote an almost-prime with at most r prime factors,counted according to multiplicity.In this paper,it is proved that,for every sufficiently large even integer N,the equation N=x^(2)+p_(2)^(2)+p_(3)^(3)+p_(4)^(... Let Pr denote an almost-prime with at most r prime factors,counted according to multiplicity.In this paper,it is proved that,for every sufficiently large even integer N,the equation N=x^(2)+p_(2)^(2)+p_(3)^(3)+p_(4)^(3)+p_(5)^(5)+_6^(5)is solvable with being an almost-prime P_(6) and the other variables primes.This result constitutes an enhancement upon the previous result of Hooley[Recent Progress in Analytic Number Theory,Vol.1(Durham,1979),London:Academic Press,1981,127-191]. 展开更多
关键词 Waring-Goldbach problem Hardy-Littlewood method almost-prime sieve method
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