Since 2011,America has maintained its position as the world’s largest natural gas producer.Since 2017,America has consistently experienced a surplus in natural gas supply,becoming a net exporter of natural gas.Americ...Since 2011,America has maintained its position as the world’s largest natural gas producer.Since 2017,America has consistently experienced a surplus in natural gas supply,becoming a net exporter of natural gas.America’s“natural gas independence”has reshaped the global natural gas market,creating a new pattern of“supply shifting westward and consumption shifting eastward”.展开更多
Wei River is an important river which affects the industrial and agricultural production,people's life in Guanzhong district of Shaanxi and the east of Gansu.To study the variation characteristics of main climate ...Wei River is an important river which affects the industrial and agricultural production,people's life in Guanzhong district of Shaanxi and the east of Gansu.To study the variation characteristics of main climate factors in recent 39 years in the upstream of Wei River,we analyzed the variation characteristics of climate factors by using the data in 11 meteorological stations in the upstream basin of Wei River during 1971-2009.The results showed that the precipitation presented the decline trend in the basin,and the temperature rose in 0.3 ℃/10 a trend.The temperature increase trend was 0.4 ℃/10 a in winter,spring and wasn't obvious in summer,autumn.The potential evapotranspiration presented the yearly increase trend in recent years.The precipitation decreased,and the temperature rose.Moreover,the potential evapotranspiration strengthened.It wasn't favorable for the healthy run of ecological system in the upstream,downstream basins and aggravated the shortage degree of water resources.展开更多
In modern gas turbines, rim seal located between the stator-disc and rotor-disc is used to prevent hot-gas ingestion into the inner stage-gap of high pressure turbine. However, the purge flow supplied to the cavity th...In modern gas turbines, rim seal located between the stator-disc and rotor-disc is used to prevent hot-gas ingestion into the inner stage-gap of high pressure turbine. However, the purge flow supplied to the cavity through the rim seal interacts with the main flow, producing additional aerodynamic loss due to the mixing process which plays a significant role in the formation, development and evolution of downstream secondary flow. In this paper, a set of cascade representative of low aspect ratio turbine is selected to numerically investigate the influence of upstream cavity purge flow on the hub secondary flow structure and aerodynamic loss. Cascade with/without upstream cavity and four different purge mass flow rates are all taken into account in this simulation. Then, a deep insight into the loss mechanism of interaction between purge flow and main flow is gained. The results show that the presence of cavity and purge flow has a significant impact on the main flow which not only changes the vortex structure in both the passage and upstream cavity, but also alters the cascade exit flow angle distribution along the spanwise. Moreover, aerodynamic loss in the cascade rises with the increase of purge flow rate while the sealing effect is also enhanced. Therefore, the effect of upstream cavity purge flow must be considered in the process of turbine aerodynamic design. What is more, it is necessary to minimize the purge flow rate in order to reduce aerodynamic loss on the premise of satisfying cooling requirements.展开更多
This paper explores the methodology for compiling the torrent hazard and risk zonation map by means of GIS technique for the Red River Basin in Yunnan province of China, where is prone to torrent. Based on a 1：250,00...This paper explores the methodology for compiling the torrent hazard and risk zonation map by means of GIS technique for the Red River Basin in Yunnan province of China, where is prone to torrent. Based on a 1：250,000 scale digital map, six factors including slope angle, rainstorm days, buffer of river channels, maximum runoff discharge of standard area, debris flow distribution density and flood disaster history were analyzed and superimposed to create the torrent risk evaluation map. Population density, farmland percentage, house property, and GDP as indexes accounting for torrent hazards were analyzed in terms of vulnerability mapping. Torrent risk zonation by means of GIS was overlaid on the two data layers of hazard and vulnerability. Then each grid unit with a resolution of 500 m- 500 m was divided into four categories of the risk： extremely high, high, moderate and low. Finally the same level risk was combined into a confirmed zone, which represents torrent risk of the study area. The risk evaluation result in the upper Red River Basin shows that the extremely high risk area of 13,150 km^2 takes up 17.9% of the total inundated area, the high risk area of 33,783 km^2 is 45.9%, the moderate risk area of 18,563 km^2 is 25.2% and the low risk area of 8115 km^2 is 11.0%.展开更多
The Explicit Upstream FEM (EU-FEM) proposed in this paper not only possesses the advantages of saving memory and CPU time for FDM, but also fits boundary easily, arranges nodal points flexibly and makes local grids fi...The Explicit Upstream FEM (EU-FEM) proposed in this paper not only possesses the advantages of saving memory and CPU time for FDM, but also fits boundary easily, arranges nodal points flexibly and makes local grids fine down conveniently. The software package, which consists of EU-FEM models and pre/post-processing skills, has been widely used to estuary, near-shore, bay, lake and complex waters with many islands and channels. In addition to flow field, this model can be used to calculate the distribution fields of pollutants, temperature, salinity, sediment, and oil spill, and can be used to case study of estuary regulation projects. Some practical applications are presented and some problems discussed.展开更多
Oil and gas exploration and production is the most important and key segment in the whole business chain of the petroleum industry.Therefore,oil companies always put much emphasis on making scientific and reasonable d...Oil and gas exploration and production is the most important and key segment in the whole business chain of the petroleum industry.Therefore,oil companies always put much emphasis on making scientific and reasonable decisions about investment scale and structure in the upstream sector,so that they can minimise business risks and obtain high returns.According to the system dynamics theories and methods and based on the actual results from an oil company＇s practice in China,a system dynamics model is built in this paper for analyzing and forecasting the upstream investment scale and structure for an oil company.This model was used to analyze the investment effect of a large oil company in China, and the results showed that the total upstream investment scale will decline slowly in a short period and the investment proportion of different parts should be adjusted if some influencing factors are taken into account.This application practice was compared with the actual data and indicated that the system dynamics（SD） model presented in this paper is a useful tool for analyzing and forecasting of upstream investment scale and structure of oil companies in their investment decisions.展开更多
Relaxation of control over the upstream business of the petroleum industry in China is discussed. The authors suggest that a basic institutional preparation should be made before relaxing control over the upstream bus...Relaxation of control over the upstream business of the petroleum industry in China is discussed. The authors suggest that a basic institutional preparation should be made before relaxing control over the upstream business, and that the institutional preparation includes at least four parts： 1） setting up the admission standards, 2） perfecting the management system of mining rights, 3） reforming the royalty and taxation system for oil and gas resources, and 4） improving the supervision and management system. Stressing the institutional preparation before relaxation of control does not mean that China could not relax control over the upstream business until the management systems are perfected, but the authors suggest that China could establish a necessary system for relaxation of control and to improve it with future practice.展开更多
All rivers in the Hexi inland region of Gansu Province, China, originate from the northern slope of the Qilian Mountains. They are located in the southern portion of the region and respectively belong to the three lar...All rivers in the Hexi inland region of Gansu Province, China, originate from the northern slope of the Qilian Mountains. They are located in the southern portion of the region and respectively belong to the three large river systems from east to west, the Shiyang, Heihe and Shule river basins. These rivers are supplied by precipitation, snowmelt and ice-melt runoff from the Qilian Mountain area. Therefore, changes of precipitation and temperature in the upstream watersheds of these rivers have an important effect on changes of mountainous runoff and reasonable utilization of water resources in this region. For this reason, the Qilian Mountain area, upstream watersheds and runoff forming areas of these rivers are chosen as the study area. The change characteristics and variation trend of temperature and precipitation in this area under the backdrop of global warming axe analyzed based on observa- tional data of relational weather and hydrologic stations in the area. Results show that temperatures in the upriver mountain areas of these three large river basins have been increasing, although the increasing degree is differentially affected by global warming. The rising extent of annual and seasonal temperatures in the upstream mountain area of the Shule river basin located in the west- em Qilian Mountains, were all largest over the past 50 years. Precipitation in the upstream mountain areas of Hexi region＇ three river basins located respectively in the western, middle and eastern Qilian Mountains have been presenting an increasing trend to varying degrees as a whole for more than 50 years. This means that climate in the upstream mountain areas of Hexi region＇ three river basins are becoming increasingly warmer and moister over the past 50 years, which will be very good for the ecological en- vironment and agricultural production in the region.展开更多
A plasma column with a length of about 65 cm is generated in the upstream region of a plasma jet using dielectric barrier discharge configurations. The effects of experimental parameters such as the amplitude of the a...A plasma column with a length of about 65 cm is generated in the upstream region of a plasma jet using dielectric barrier discharge configurations. The effects of experimental parameters such as the amplitude of the applied voltage and the driving frequency are investigated in aspects of the plasma column by the optical method. Results show that both the plasma length and the propagating velocity, as well as the discharge current, increase with the increase in the applied voltage or its frequency. The discharge mechanism is analysed qualitatively based on streamer theory, where photo-ionization is important. Furthermore, optical emission spectroscopy is used to investigate the electric field intensity of the upstream region.展开更多
Making a brief analysis of the water and soil loss present situation in Daxing＇anling area which locates to the upstream region of Nenjiang River, and giving the water and soil loss of this area that have been made n...Making a brief analysis of the water and soil loss present situation in Daxing＇anling area which locates to the upstream region of Nenjiang River, and giving the water and soil loss of this area that have been made near 20 years, as well as the factors of the water and soil loss. According to the factors corresponding prevention measure and forecast model have been put forward, make a brief introduction to this model in this article. It is helpful to improve the local soil conservation and sustainable development.展开更多
This paper deals with a first-principle mathematical model that describes the electrostatic coalescer unitsdevoted to the separation of water from oil in water-in-oil emulsions, which are typical of the upstreamoperat...This paper deals with a first-principle mathematical model that describes the electrostatic coalescer unitsdevoted to the separation of water from oil in water-in-oil emulsions, which are typical of the upstreamoperations in oil fields. The main phenomena governing the behavior of the electrostatic coalescer are denscribed, starting from fundamental laws. In addition, the gradual coalescence of the emulsion droplets isconsidered in the mathematical modeling in a dynamic fashion, as the phenomenon is identified as a keystep in the overall yield of the unit operation. The resulting differential system with boundary conditionsis then integrated via performing numerical libraries, and the simulation results confirm the available lit-erature and the industrial data. A sensitivity analysis is provided with respect to the main parameters. Themathematical model results in a flexible tool that is useful for the purposes of design, unit behavior predic-tion. performance rnoniroring, and ontimization.展开更多
It is suggested that Δ6 fatty acid desaturase(FAD) plays a critical role in the biosynthesis of polyunsaturated fatty acids in plants and microalgae. But why does it adapt to the changed environments such as nitrogen...It is suggested that Δ6 fatty acid desaturase(FAD) plays a critical role in the biosynthesis of polyunsaturated fatty acids in plants and microalgae. But why does it adapt to the changed environments such as nitrogen starvation is seldom understood. One Δ6 FAD gene( MiD6 fad) from an arachidonic acidrich microalga M yrmecia incisa Reisigl(Chlorophyta) was first heterologously expressed in S accharomyces cerevisiae for the identification of function. The fatty acid profile of transgenic yeast detected by gas chromatography-mass spectrometry illustrated that the enzyme MiD6 FAD could convert linoleic and ?-linolenic acids to γ-linolenic and stearidonic acids, respectively, demonstrating that M iD6 fad encoded a Δ6 FAD. A 1 965-bp fragment of the cloned 2 347-bp 5′-upstream region of M iD6 fad was next subcloned and fused upstream with green fluorescent protein(GFP) gene to replace the GAL1 promoter of the vector pYES2. The generated construct was transformed into S. cerevisiae for function determination. Confocal microscopic images of the transformed line illustrated that this inserted fragment could drive GFP expression, which was further verified by fluorescence intensity quantification and Western blot analysis using antiGFP antibody. The conversion efficiency(approximately 2%-3%) of MiD6 FAD was much lower than the reported ? 3 FAD and Δ6 elongase in this microalga, suggesting that MiD6 FAD catalysed the possible ratelimiting step for ArA biosynthesis. The presence of several putative c is-acting regulatory elements in this identified promoter sheds new light on the regulation mechanism research of Δ6 FAD transcription for the ArA production in M. incisa in changing environmental factors.展开更多
Numerous environmental and endogenous signals control the highly orchestrated and intricate process of plant senescence.Ethylene,a well-known inducer of senescence,has long been considered a key endogenous regulator o...Numerous environmental and endogenous signals control the highly orchestrated and intricate process of plant senescence.Ethylene,a well-known inducer of senescence,has long been considered a key endogenous regulator of leaf and flower senescence,but the molecular mechanism of ethylene-induced ovule senescence has not yet been elucidated.In this study,we found that blockage of fertilization caused ovule abortion in the pear cultivar‘1913’.According to transcriptome and phytohormone content data,ethylene biosynthesis was activated by pollination.At the same time,ethylene overaccumulated in ovules,where cells were sensitive to ethylene signals in the absence of fertilization.We identified a transcription factor in the ethylene signal response,ethylene-insensitive 3-like(EIL1),as a likely participant in ovule senescence.Overexpression of PbEIL1 in tomato caused precocious onset of ovule senescence.We further found that EIL1 could directly bind to the promoter of the SENESCENCE-ASSOCIATED CYSTEINE PROTEINASE 1(PbCysp1)gene and act upstream of senescence.Yeast one-hybrid and dual-luciferase assays revealed the interaction of the transcription factor and the promoter DNA sequence and demonstrated that PbEIL1 enhanced the action of PbCysp1.Collectively,our results provide new insights into how ethylene promotes the progression of unfertilized ovule senescence.展开更多
Upstream-downstream relationships of annual streamflow discharges and severity and frequency of stream-flow drought events are critical in understanding how streamflow droughts propagate over time and space. Such info...Upstream-downstream relationships of annual streamflow discharges and severity and frequency of stream-flow drought events are critical in understanding how streamflow droughts propagate over time and space. Such information can be used to resolve water disputes, trigger mitigation strategies, and understand how streamflow changes due to changes in the environment. During drought years, such information is even more critical as water resources are contested. The objective of this research is to study the upstream-downstream relationships of streamflow in Nebraska along four major river systems with diverse hydrologic characteris-tics and human activities: North Platte, Big Blue, Republican, and Niobrara. The relationships among the upstream and downstream stations along the four rivers are investigated by comparing several statistics de-rived from the annual flow discharge and on drought events. Trend analysis and coefficient of variation are applied to annual flow discharge values, and a host of drought-related parameters (e.g., annual maximum drought duration, annual accumulated drought duration, number of drought events) are also computed with respect to five different levels of streamflow drought events: water shortage, mild drought, moderate drought, significant drought, and extreme drought. The paired-t test and ANOVA with MIXED procedure are subse-quently applied to the statistics to observe whether there is a significant difference between upstream and downstream stations along a river. The analysis allows us to characterize the upstream-downstream relation-ships of the four river systems, laying the groundwork for further investigations to identify the reasons for some of the trends and observations. These findings will be essential in water resources management during or prior to hydrological droughts.展开更多
文摘Since 2011,America has maintained its position as the world’s largest natural gas producer.Since 2017,America has consistently experienced a surplus in natural gas supply,becoming a net exporter of natural gas.America’s“natural gas independence”has reshaped the global natural gas market,creating a new pattern of“supply shifting westward and consumption shifting eastward”.
基金Supported by "Ten People Plan" of Gansu Meteorological BureauPublic Welfare Industry Special Item of National Science Technology Department (GYHY200806021)
文摘Wei River is an important river which affects the industrial and agricultural production,people's life in Guanzhong district of Shaanxi and the east of Gansu.To study the variation characteristics of main climate factors in recent 39 years in the upstream of Wei River,we analyzed the variation characteristics of climate factors by using the data in 11 meteorological stations in the upstream basin of Wei River during 1971-2009.The results showed that the precipitation presented the decline trend in the basin,and the temperature rose in 0.3 ℃/10 a trend.The temperature increase trend was 0.4 ℃/10 a in winter,spring and wasn't obvious in summer,autumn.The potential evapotranspiration presented the yearly increase trend in recent years.The precipitation decreased,and the temperature rose.Moreover,the potential evapotranspiration strengthened.It wasn't favorable for the healthy run of ecological system in the upstream,downstream basins and aggravated the shortage degree of water resources.
基金Key Laboratory Foundation (9140C4103091003C) for funding this work
文摘In modern gas turbines, rim seal located between the stator-disc and rotor-disc is used to prevent hot-gas ingestion into the inner stage-gap of high pressure turbine. However, the purge flow supplied to the cavity through the rim seal interacts with the main flow, producing additional aerodynamic loss due to the mixing process which plays a significant role in the formation, development and evolution of downstream secondary flow. In this paper, a set of cascade representative of low aspect ratio turbine is selected to numerically investigate the influence of upstream cavity purge flow on the hub secondary flow structure and aerodynamic loss. Cascade with/without upstream cavity and four different purge mass flow rates are all taken into account in this simulation. Then, a deep insight into the loss mechanism of interaction between purge flow and main flow is gained. The results show that the presence of cavity and purge flow has a significant impact on the main flow which not only changes the vortex structure in both the passage and upstream cavity, but also alters the cascade exit flow angle distribution along the spanwise. Moreover, aerodynamic loss in the cascade rises with the increase of purge flow rate while the sealing effect is also enhanced. Therefore, the effect of upstream cavity purge flow must be considered in the process of turbine aerodynamic design. What is more, it is necessary to minimize the purge flow rate in order to reduce aerodynamic loss on the premise of satisfying cooling requirements.
基金National Natural Science Foundation of China, No.40371018
文摘This paper explores the methodology for compiling the torrent hazard and risk zonation map by means of GIS technique for the Red River Basin in Yunnan province of China, where is prone to torrent. Based on a 1：250,000 scale digital map, six factors including slope angle, rainstorm days, buffer of river channels, maximum runoff discharge of standard area, debris flow distribution density and flood disaster history were analyzed and superimposed to create the torrent risk evaluation map. Population density, farmland percentage, house property, and GDP as indexes accounting for torrent hazards were analyzed in terms of vulnerability mapping. Torrent risk zonation by means of GIS was overlaid on the two data layers of hazard and vulnerability. Then each grid unit with a resolution of 500 m- 500 m was divided into four categories of the risk： extremely high, high, moderate and low. Finally the same level risk was combined into a confirmed zone, which represents torrent risk of the study area. The risk evaluation result in the upper Red River Basin shows that the extremely high risk area of 13,150 km^2 takes up 17.9% of the total inundated area, the high risk area of 33,783 km^2 is 45.9%, the moderate risk area of 18,563 km^2 is 25.2% and the low risk area of 8115 km^2 is 11.0%.
文摘The Explicit Upstream FEM (EU-FEM) proposed in this paper not only possesses the advantages of saving memory and CPU time for FDM, but also fits boundary easily, arranges nodal points flexibly and makes local grids fine down conveniently. The software package, which consists of EU-FEM models and pre/post-processing skills, has been widely used to estuary, near-shore, bay, lake and complex waters with many islands and channels. In addition to flow field, this model can be used to calculate the distribution fields of pollutants, temperature, salinity, sediment, and oil spill, and can be used to case study of estuary regulation projects. Some practical applications are presented and some problems discussed.
文摘Oil and gas exploration and production is the most important and key segment in the whole business chain of the petroleum industry.Therefore,oil companies always put much emphasis on making scientific and reasonable decisions about investment scale and structure in the upstream sector,so that they can minimise business risks and obtain high returns.According to the system dynamics theories and methods and based on the actual results from an oil company＇s practice in China,a system dynamics model is built in this paper for analyzing and forecasting the upstream investment scale and structure for an oil company.This model was used to analyze the investment effect of a large oil company in China, and the results showed that the total upstream investment scale will decline slowly in a short period and the investment proportion of different parts should be adjusted if some influencing factors are taken into account.This application practice was compared with the actual data and indicated that the system dynamics（SD） model presented in this paper is a useful tool for analyzing and forecasting of upstream investment scale and structure of oil companies in their investment decisions.
文摘Relaxation of control over the upstream business of the petroleum industry in China is discussed. The authors suggest that a basic institutional preparation should be made before relaxing control over the upstream business, and that the institutional preparation includes at least four parts： 1） setting up the admission standards, 2） perfecting the management system of mining rights, 3） reforming the royalty and taxation system for oil and gas resources, and 4） improving the supervision and management system. Stressing the institutional preparation before relaxation of control does not mean that China could not relax control over the upstream business until the management systems are perfected, but the authors suggest that China could establish a necessary system for relaxation of control and to improve it with future practice.
基金supported by the Open Foundation of Key Laboratory of Ecohydrology in Inland River Basin, Chinese Academy of Sciences and National Nature Science Foundation of China (No. 9112502)The Coupling and Modeling of Eco-hydrological Processes in the Upper Reaches of Heihe River (No. 91225302)+1 种基金Research of Eco-hydrological Response Units in Heihe River Basin Based on the Method of Concept Lattice (No. 41240002)System Behaviors and Regulation of Ecohydrological Processes in the Middle and Lower Heihe River Basin (91225301)
文摘All rivers in the Hexi inland region of Gansu Province, China, originate from the northern slope of the Qilian Mountains. They are located in the southern portion of the region and respectively belong to the three large river systems from east to west, the Shiyang, Heihe and Shule river basins. These rivers are supplied by precipitation, snowmelt and ice-melt runoff from the Qilian Mountain area. Therefore, changes of precipitation and temperature in the upstream watersheds of these rivers have an important effect on changes of mountainous runoff and reasonable utilization of water resources in this region. For this reason, the Qilian Mountain area, upstream watersheds and runoff forming areas of these rivers are chosen as the study area. The change characteristics and variation trend of temperature and precipitation in this area under the backdrop of global warming axe analyzed based on observa- tional data of relational weather and hydrologic stations in the area. Results show that temperatures in the upriver mountain areas of these three large river basins have been increasing, although the increasing degree is differentially affected by global warming. The rising extent of annual and seasonal temperatures in the upstream mountain area of the Shule river basin located in the west- em Qilian Mountains, were all largest over the past 50 years. Precipitation in the upstream mountain areas of Hexi region＇ three river basins located respectively in the western, middle and eastern Qilian Mountains have been presenting an increasing trend to varying degrees as a whole for more than 50 years. This means that climate in the upstream mountain areas of Hexi region＇ three river basins are becoming increasingly warmer and moister over the past 50 years, which will be very good for the ecological en- vironment and agricultural production in the region.
基金supported by the National Natural Science Foundation of China (Grant Nos. 10805013 and 51077035)the Natural Science Foundation of Hebei Province, China (Grant Nos. A2009000149 and A2011201132)+2 种基金the Key Project of Chinese Ministry of Education, China (Grant No. 210014)the Science Funds of Hebei Education Department for Outstanding Youth (Grant No. Y2011120)the Hebei University Funds for Distinguished Young Scientists
文摘A plasma column with a length of about 65 cm is generated in the upstream region of a plasma jet using dielectric barrier discharge configurations. The effects of experimental parameters such as the amplitude of the applied voltage and the driving frequency are investigated in aspects of the plasma column by the optical method. Results show that both the plasma length and the propagating velocity, as well as the discharge current, increase with the increase in the applied voltage or its frequency. The discharge mechanism is analysed qualitatively based on streamer theory, where photo-ionization is important. Furthermore, optical emission spectroscopy is used to investigate the electric field intensity of the upstream region.
基金Supportde by The Natual Science Fund of Heilongjiang Province(E0335)
文摘Making a brief analysis of the water and soil loss present situation in Daxing＇anling area which locates to the upstream region of Nenjiang River, and giving the water and soil loss of this area that have been made near 20 years, as well as the factors of the water and soil loss. According to the factors corresponding prevention measure and forecast model have been put forward, make a brief introduction to this model in this article. It is helpful to improve the local soil conservation and sustainable development.
文摘This paper deals with a first-principle mathematical model that describes the electrostatic coalescer unitsdevoted to the separation of water from oil in water-in-oil emulsions, which are typical of the upstreamoperations in oil fields. The main phenomena governing the behavior of the electrostatic coalescer are denscribed, starting from fundamental laws. In addition, the gradual coalescence of the emulsion droplets isconsidered in the mathematical modeling in a dynamic fashion, as the phenomenon is identified as a keystep in the overall yield of the unit operation. The resulting differential system with boundary conditionsis then integrated via performing numerical libraries, and the simulation results confirm the available lit-erature and the industrial data. A sensitivity analysis is provided with respect to the main parameters. Themathematical model results in a flexible tool that is useful for the purposes of design, unit behavior predic-tion. performance rnoniroring, and ontimization.
基金Supported by the National Natural Science Foundation of China(No.31172389)the Special Project of Marine Renewable Energy from the State Oceanic Administration(No.SHME2011SW02)the Shanghai Universities Peak Discipline Project of Aquaculture
文摘It is suggested that Δ6 fatty acid desaturase(FAD) plays a critical role in the biosynthesis of polyunsaturated fatty acids in plants and microalgae. But why does it adapt to the changed environments such as nitrogen starvation is seldom understood. One Δ6 FAD gene( MiD6 fad) from an arachidonic acidrich microalga M yrmecia incisa Reisigl(Chlorophyta) was first heterologously expressed in S accharomyces cerevisiae for the identification of function. The fatty acid profile of transgenic yeast detected by gas chromatography-mass spectrometry illustrated that the enzyme MiD6 FAD could convert linoleic and ?-linolenic acids to γ-linolenic and stearidonic acids, respectively, demonstrating that M iD6 fad encoded a Δ6 FAD. A 1 965-bp fragment of the cloned 2 347-bp 5′-upstream region of M iD6 fad was next subcloned and fused upstream with green fluorescent protein(GFP) gene to replace the GAL1 promoter of the vector pYES2. The generated construct was transformed into S. cerevisiae for function determination. Confocal microscopic images of the transformed line illustrated that this inserted fragment could drive GFP expression, which was further verified by fluorescence intensity quantification and Western blot analysis using antiGFP antibody. The conversion efficiency(approximately 2%-3%) of MiD6 FAD was much lower than the reported ? 3 FAD and Δ6 elongase in this microalga, suggesting that MiD6 FAD catalysed the possible ratelimiting step for ArA biosynthesis. The presence of several putative c is-acting regulatory elements in this identified promoter sheds new light on the regulation mechanism research of Δ6 FAD transcription for the ArA production in M. incisa in changing environmental factors.
基金the National Key R&D Program of China(2019YFD1001400)the China Agriculture Research System(CARS 28-45).
文摘Numerous environmental and endogenous signals control the highly orchestrated and intricate process of plant senescence.Ethylene,a well-known inducer of senescence,has long been considered a key endogenous regulator of leaf and flower senescence,but the molecular mechanism of ethylene-induced ovule senescence has not yet been elucidated.In this study,we found that blockage of fertilization caused ovule abortion in the pear cultivar‘1913’.According to transcriptome and phytohormone content data,ethylene biosynthesis was activated by pollination.At the same time,ethylene overaccumulated in ovules,where cells were sensitive to ethylene signals in the absence of fertilization.We identified a transcription factor in the ethylene signal response,ethylene-insensitive 3-like(EIL1),as a likely participant in ovule senescence.Overexpression of PbEIL1 in tomato caused precocious onset of ovule senescence.We further found that EIL1 could directly bind to the promoter of the SENESCENCE-ASSOCIATED CYSTEINE PROTEINASE 1(PbCysp1)gene and act upstream of senescence.Yeast one-hybrid and dual-luciferase assays revealed the interaction of the transcription factor and the promoter DNA sequence and demonstrated that PbEIL1 enhanced the action of PbCysp1.Collectively,our results provide new insights into how ethylene promotes the progression of unfertilized ovule senescence.
文摘Upstream-downstream relationships of annual streamflow discharges and severity and frequency of stream-flow drought events are critical in understanding how streamflow droughts propagate over time and space. Such information can be used to resolve water disputes, trigger mitigation strategies, and understand how streamflow changes due to changes in the environment. During drought years, such information is even more critical as water resources are contested. The objective of this research is to study the upstream-downstream relationships of streamflow in Nebraska along four major river systems with diverse hydrologic characteris-tics and human activities: North Platte, Big Blue, Republican, and Niobrara. The relationships among the upstream and downstream stations along the four rivers are investigated by comparing several statistics de-rived from the annual flow discharge and on drought events. Trend analysis and coefficient of variation are applied to annual flow discharge values, and a host of drought-related parameters (e.g., annual maximum drought duration, annual accumulated drought duration, number of drought events) are also computed with respect to five different levels of streamflow drought events: water shortage, mild drought, moderate drought, significant drought, and extreme drought. The paired-t test and ANOVA with MIXED procedure are subse-quently applied to the statistics to observe whether there is a significant difference between upstream and downstream stations along a river. The analysis allows us to characterize the upstream-downstream relation-ships of the four river systems, laying the groundwork for further investigations to identify the reasons for some of the trends and observations. These findings will be essential in water resources management during or prior to hydrological droughts.
