摘要
目的探讨上游转录因子1(USF1)在肝细胞癌(HCC)细胞增殖中的作用。方法通过基于TCGA数据库的泛癌分析探讨HCC患者肿瘤组织和癌旁正常肝组织中USF1 mRNA相对表达水平的差异,通过免疫组化和免疫荧光染色检测HCC患者肿瘤组织和癌旁正常肝组织中USF1蛋白表达水平。将人HCC细胞系Hep3B细胞分为A1~D1组,Huh7细胞分为A2~D2组,其中A1和A2组细胞不加入任何试剂处理,其余细胞分别转染siNC(B1、B2组)、siUSF1#1(C1、C2组)和siUSF1#2(D1、D2组),通过CCK-8实验检测各组细胞的细胞活力,通过集落形成实验检测各组细胞的克隆形成能力,通过EdU染色检测各组细胞的增殖能力。结果基于TCGA数据库的泛癌分析结果显示,与癌旁正常肝组织相比,HCC患者肿瘤组织中USF1 mRNA相对表达水平显著上调(t=6.84,P<0.05)。免疫组化及免疫荧光染色结果显示,与癌旁正常肝组织相比,HCC患者肿瘤组织中USF1的表达水平显著升高。CCK-8实验结果显示,培养第48~96小时时,C1、D1组和C2、D2组细胞活力均分别显著低于B1和B2组(F=4.74~413.67,q=2.89~31.86,P<0.05)。集落形成实验和EdU染色结果显示,C1、C2组和D1、D2组细胞集落数目和EdU阳性细胞比例显著低于B1和B2组(F=113.87~390.29,q=18.37~34.42,P<0.05)。结论USF1在HCC患者肿瘤组织中表达上调,抑制USF1的表达可能抑制HCC细胞的增殖。
Objective To investigate the role of upstream stimulatory factor 1(USF1)in the proliferation of hepatocellular carcinoma(HCC)cells.Methods A pan-cancer analysis based on the TCGA database was performed to compare the relative mRNA expression levels of USF1 between tumor tissues and adjacent normal liver tissues from patients with HCC.Immunohistochemistry and immunofluorescence staining were used to assess USF1 protein expression in tumor tissues and adjacent normal tissues.Hep3B cells were divided into groups A1-D1,and Huh7 cells into groups A2-D2.Cells in groups A1 and A2 received no treatment;the remaining cells were transfected with siNC(groups B1 and B2),siUSF1#1(groups C1 and C2),or siUSF1#2(groups D1 and D2).Cell viability was measured using the CCK-8 assay,colony-forming ability using the colony formation assay,and proliferation capacity using EdU staining.Results Pan-cancer analysis based on the TCGA database showed that the relative mRNA expression level of USF1 was significantly higher in HCC tumor tissues than in adjacent normal liver tissues(t=6.84,P<0.05).Immunochemistry and immunofluorescence staining similarly showed significantly increased USF1 protein expression in tumor tissues.According to the CCK-8 assay,at 48-96 h of culture,cell viability in groups C1 and D1 and groups C2 and D2 was significantly lower than that in groups B1 and B2,respectively(F=4.74-413.67,q=2.89-31.86,P<0.05).Colony formation and EdU assays showed that the numbers of colonies and the proportions of EdU-positive cells in groups C1 and C2 and groups D1 and D2 were significantly lower than those in groups B1 and B2(F=113.87-390.29,q=18.37-34.42,P<0.05).Conclusion USF1 expression is upregulated in the tumor tissues of patients with HCC,and suppressing USF1 expression may inhibit the proliferation of HCC cells.
作者
李欢腾
郭灿灿
蔡铎
宋妤
贺桂芳
李鑫
王朋
刘世海
LI Huanteng;GUO Cancan;CAI Duo;SONG Yu;HE Guifang;LI Xin;WANG Peng;LIU Shihai(Department of Clinical Laboratory,The Affiliated Hospital of Qingdao University,Qingdao 266003,China)
出处
《精准医学杂志》
2026年第1期1-7,共7页
Journal of Precision Medicine
基金
山东省自然科学基金面上项目(ZR2021MH022)。
关键词
癌
肝细胞
细胞增殖
上游刺激因子类
细胞运动
上游转录因子1
Carcinoma,hepatocellular
Cell proliferation
Upstream stimulatory factors
Cell movement
Upstream stimulatory factor 1
