5-hydroxytryptamine(5-HT)is a biological monoamine neurotransmitter in the central nervous system and gonads of crustaceans to induce gonadal maturation.To better understand the effects of 5-HT and its receptors on re...5-hydroxytryptamine(5-HT)is a biological monoamine neurotransmitter in the central nervous system and gonads of crustaceans to induce gonadal maturation.To better understand the effects of 5-HT and its receptors on reproductive development in Artemia,a 5-HT type 7 receptor gene(5-HT_(7Ar))was identified in parthenogenetic Artemia and characterized.Sequence analysis revealed that the open reading frame of 5-HT_(7Ar) encodes a 414-aa protein.5-HT_(7Ar) showed higher expression in both brain and ovary at early embryo stage,5-HT_(7Ar) could be detected during ovarian development and the highest expression was observed at early embryo stage.Silencing of the 5-HT_(7Ar) in Artemia at early embryo stage decreased significantly the expression level of 5-HT_(7Ar) gene and protein at late oocyte,early embryo,and late embryo stage.Moreover,silencing of the 5-HT_(7Ar) resulted in a decreased fecundity,which corresponds to abnormal oocytes during the embryo development.Artemia tended to produce nauplii after the silencing of 5-HT_(7Ar),indicating that 5-HT_(7Ar) may also involve in the determination of its reproduction mode.The findings of this study provide an insight into the regulation of reproductive development in Artemia and the function of 5-HT_(7Ar).展开更多
基金Supported by the Tianjin Key Laboratory of Marine Resources and Chemistry(Tianjin University of Science&Technology),Tianjin,China(No.202302)the National Natural Science Foundation of China(No.32460154)the China Postdoctoral Science Foundation(No.2023MD734227)。
文摘5-hydroxytryptamine(5-HT)is a biological monoamine neurotransmitter in the central nervous system and gonads of crustaceans to induce gonadal maturation.To better understand the effects of 5-HT and its receptors on reproductive development in Artemia,a 5-HT type 7 receptor gene(5-HT_(7Ar))was identified in parthenogenetic Artemia and characterized.Sequence analysis revealed that the open reading frame of 5-HT_(7Ar) encodes a 414-aa protein.5-HT_(7Ar) showed higher expression in both brain and ovary at early embryo stage,5-HT_(7Ar) could be detected during ovarian development and the highest expression was observed at early embryo stage.Silencing of the 5-HT_(7Ar) in Artemia at early embryo stage decreased significantly the expression level of 5-HT_(7Ar) gene and protein at late oocyte,early embryo,and late embryo stage.Moreover,silencing of the 5-HT_(7Ar) resulted in a decreased fecundity,which corresponds to abnormal oocytes during the embryo development.Artemia tended to produce nauplii after the silencing of 5-HT_(7Ar),indicating that 5-HT_(7Ar) may also involve in the determination of its reproduction mode.The findings of this study provide an insight into the regulation of reproductive development in Artemia and the function of 5-HT_(7Ar).
