摘要
铁氧还蛋白-NADP+氧化还原酶(FNR)是一种重要的酶,它在植物光合作用和细胞呼吸过程中发挥关键作用。本研究以普通小麦品种中国春为材料,利用RT-PCR技术克隆获得TaLFNR1-7A基因,对其进行生物信息学和基因表达分析,结果表明,TaLFNR1-7A基因CDS序列长度为1086 bp,编码361个氨基酸;其编码的蛋白质为一个非跨膜的稳定的亲水性蛋白质,无信号肽,共含39个磷酸化位点;预测其定位于叶绿体;系统进化树以及氨基酸序列多重比对发现,基于TaLFNR1-7A基因普通小麦中国春与二粒小麦在亲缘性上更为接近,相似度为97.01%。启动子顺式作用元件分析结果显示,TaLFNR1-7A基因中共含有17种顺式作用元件,其中7个为光响应元件。经qRT-PCR分析发现,TaLFNR1-7A基因在小麦叶片中表达量最高,其次是茎中和胚芽中,根中表达量最低。TaLFNR1-7A基因响应多种非生物胁迫,这些非生物胁迫包括干旱(PEG-6000处理)、茉莉酸甲酯(MeJA)、氯化钠(NaCl)和脱落酸(ABA)等。本研究结果为进一步探讨TaLFNR1-7A基因在小麦生长发育过程中的作用机制提供了重要参考。
Ferritin-NADP+reductase(FNR)is an important enzyme that plays a crucial role in photosynthesis and cellular respiration processes.This study used common wheat Chinese Spring as the material and cloned the TaLFNR1-7A gene using RT-PCR technology.Bioinformatics and gene expression analysis were performed on it.The results showed that the CDS sequence length of TaLFNR1-7A gene was 1086 bp,encoding 361 amino acids.The encoded protein was a non-transmembrane stable hydrophilic protein,without a signal peptide,containing a total of 39 phosphorylation sites.It was predicted to be localized in the chloroplast.Phylogenetic analysis and multiple amino acid sequence alignment revealed that common wheat(Chinese Spring)and emmer wheat exhibited closer phylogenetic proximity based on the TaLFNR1-7A gene,with a sequence similarity of 97.01%.Analysis of promoter cis-acting elements revealed that the TaLFNR1-7A gene contained 17 cis-acting elements,including seven light-responsive elements.The qRT-PCR analysis revealed that the expression of TaLFNR1-7A gene was highest in wheat leaves,followed by stems and germs,with the lowest level observed in roots.The TaLFNR1-7A gene responded to multiple abiotic stresses,including drought(PEG-6000 treatment),methyl jasmonate(MeJA),sodium chloride(NaCl),and abscisic acid(ABA).The findings of this study provide a significant foundation for further exploring the functional mechanisms of the TaLFNR1-7A gene in wheat growth and development.
作者
邓云颢
徐灵
李鲁华
宋婵
徐如宏
DENG Yunhao;XU Ling;LI Luhua;SONG Chan;XU Ruhong(College of Agriculture,Guizhou University,Guiyang 550025,China;Guizhou Branch of National Wheat Improvement Center,Guiyang 550025,China)
出处
《江苏农业学报》
北大核心
2025年第6期1041-1049,共9页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(32360474、32160456、32360486)
贵州省科技支撑计划项目[黔科合支撑(2021)一般272]
贵州省粮油作物分子育种重点实验室项目[黔科合中引地(2023)008]
贵州省高等学校功能农业重点实验室项目[黔教技(2023)007号]。
