Hop samples from different region in Xinjiang were collected and detected by reverse transcription-polymerase chain reaction(RT-PCR) in order to determine the occurrence of HpLV.The RT-PCR products were 314 bp and 964...Hop samples from different region in Xinjiang were collected and detected by reverse transcription-polymerase chain reaction(RT-PCR) in order to determine the occurrence of HpLV.The RT-PCR products were 314 bp and 964 bp.Coat protein(CP) gene segments of isolates AW3 from Wensu,CH1 from Changji,K6 from Yanqi,SW8 from Shawan,TE2 from Emin,TY4 from Tacheng and YL4 from Yili were cloned.Nucleic acid sequence of the CP gene and deduced CP amino acid sequence alignments were compared with AB032469 registered in GenBank.The result showed that 8 HpLV isolates were divided into two groups.Among the isolates,AB032469,K6,AW3,CH1,TE2,TY4 and YL4 were classified as GroupⅠand SW8 was assigned to GroupⅡ.GroupⅠisolates fell into three small clusters.Nucleotide and deduced amino acid sequence comparisons within GroupⅠisolates showed that the sequence homologies were 98.1%-99.9% and 99.0%-100%,respectively. Comparisons of nucleotide and deduced amino acid sequences between Group Ⅰ and Group Ⅱ showed that the sequence homologies were 95.8%-96.4% and 98.4%-99.0%,respectively.Amino acid identity was higher than nucleic acid identity among all isolates.展开更多
文摘Hop samples from different region in Xinjiang were collected and detected by reverse transcription-polymerase chain reaction(RT-PCR) in order to determine the occurrence of HpLV.The RT-PCR products were 314 bp and 964 bp.Coat protein(CP) gene segments of isolates AW3 from Wensu,CH1 from Changji,K6 from Yanqi,SW8 from Shawan,TE2 from Emin,TY4 from Tacheng and YL4 from Yili were cloned.Nucleic acid sequence of the CP gene and deduced CP amino acid sequence alignments were compared with AB032469 registered in GenBank.The result showed that 8 HpLV isolates were divided into two groups.Among the isolates,AB032469,K6,AW3,CH1,TE2,TY4 and YL4 were classified as GroupⅠand SW8 was assigned to GroupⅡ.GroupⅠisolates fell into three small clusters.Nucleotide and deduced amino acid sequence comparisons within GroupⅠisolates showed that the sequence homologies were 98.1%-99.9% and 99.0%-100%,respectively. Comparisons of nucleotide and deduced amino acid sequences between Group Ⅰ and Group Ⅱ showed that the sequence homologies were 95.8%-96.4% and 98.4%-99.0%,respectively.Amino acid identity was higher than nucleic acid identity among all isolates.
