The plant aerial parts of three species, Urtica dioica L., Viola odorata L. and Melissa officinalis L. were collected at randomly-different locations, according to altitudes in May 2010. The aerial parts of Urtica wer...The plant aerial parts of three species, Urtica dioica L., Viola odorata L. and Melissa officinalis L. were collected at randomly-different locations, according to altitudes in May 2010. The aerial parts of Urtica were collected within three replications from different locations： Biare 1,090 masl （meters above sea level）, Tawile 1,450 masl and Awiser 1,680 masl. The aerial parts of Viola and Melissa were collected randomly within three replications at different locations： Biare 1,090 masl, Degashikhan 1,250 masl and Tawile 1,450 masl. The extracts of the aerial parts of these species were purified by filtrations for several times in preparation for HPLC analyses. The chromatograms of Urtica indicated the presence of five major important alkaloid components （fragrine, benzylisoquinoline, scopoletin, glucoquinone and dotriacotaine） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. All the concentrations of alkaloid and phenolic compounds were increased significantly due to higher altitudes, except that of alkaloid dotriacotaine. The chromatograms of Viola indicated the presence of four major important alkaloid components （violine, isoquinoline, cycloviolacin and luteolin-3-glucoronide） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. The results of the influence of altitudes showed that the concentrations of all alkaloids and phenolic compounds were increased significantly due to higher altitudes, except that of the alkaloid luteolin-3-glucoronide and the phenolic compounds zeaxanthin and luetin epoxide. The chromatograms of Melissa indicated the presence of five major essential oils （pinene, linalool, citronellol, geraniol and rosmarinic acid）. Their quantitative evaluations were influenced by altitudes indicating that the concentrations of all oils were increased significantly due to the higher altitude, except that of the pinene.展开更多
文摘The plant aerial parts of three species, Urtica dioica L., Viola odorata L. and Melissa officinalis L. were collected at randomly-different locations, according to altitudes in May 2010. The aerial parts of Urtica were collected within three replications from different locations： Biare 1,090 masl （meters above sea level）, Tawile 1,450 masl and Awiser 1,680 masl. The aerial parts of Viola and Melissa were collected randomly within three replications at different locations： Biare 1,090 masl, Degashikhan 1,250 masl and Tawile 1,450 masl. The extracts of the aerial parts of these species were purified by filtrations for several times in preparation for HPLC analyses. The chromatograms of Urtica indicated the presence of five major important alkaloid components （fragrine, benzylisoquinoline, scopoletin, glucoquinone and dotriacotaine） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. All the concentrations of alkaloid and phenolic compounds were increased significantly due to higher altitudes, except that of alkaloid dotriacotaine. The chromatograms of Viola indicated the presence of four major important alkaloid components （violine, isoquinoline, cycloviolacin and luteolin-3-glucoronide） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. The results of the influence of altitudes showed that the concentrations of all alkaloids and phenolic compounds were increased significantly due to higher altitudes, except that of the alkaloid luteolin-3-glucoronide and the phenolic compounds zeaxanthin and luetin epoxide. The chromatograms of Melissa indicated the presence of five major essential oils （pinene, linalool, citronellol, geraniol and rosmarinic acid）. Their quantitative evaluations were influenced by altitudes indicating that the concentrations of all oils were increased significantly due to the higher altitude, except that of the pinene.
