1-Aminocyclopropane-1-carboxylic acid synthase （ACS） and 1-aminocyclopropane-1-carboxylic acid oxidase （ACO） are encoded by multigene families and are involved in fruit ripening by catalyzing the production of eth...1-Aminocyclopropane-1-carboxylic acid synthase （ACS） and 1-aminocyclopropane-1-carboxylic acid oxidase （ACO） are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type I, MaACS3 and MaACS4 are type II, and MaACS5 is type III, with different C-terminal se-quences. Quantitative reverse transcriptase polymerase chain reaction （qRT-PCR） expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demon-strated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid （ABA） and ethephon.展开更多
基金Project supported by the National Natural Science Foundation of China(No.31360190)the Special Fund for Agro-scientific Research in the Public Interest of China(No.201403064)the China Agriculture Research System(No.CARS-22-ZJ0102)
文摘1-Aminocyclopropane-1-carboxylic acid synthase （ACS） and 1-aminocyclopropane-1-carboxylic acid oxidase （ACO） are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type I, MaACS3 and MaACS4 are type II, and MaACS5 is type III, with different C-terminal se-quences. Quantitative reverse transcriptase polymerase chain reaction （qRT-PCR） expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demon-strated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid （ABA） and ethephon.
