The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). T...The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.展开更多
报导了不同基质和不同药剂浓度(G_y)对银杏扦插生根成活的影响。其中以2100ppmG,最佳,1500pm 次之,基质以砂土较好,最优处理组合是砂土+2100pmG_y,移栽时用100ppmNAA+助剂 B 能显著提高移栽成活率,还对扦插生根、成活及移栽成活进行了...报导了不同基质和不同药剂浓度(G_y)对银杏扦插生根成活的影响。其中以2100ppmG,最佳,1500pm 次之,基质以砂土较好,最优处理组合是砂土+2100pmG_y,移栽时用100ppmNAA+助剂 B 能显著提高移栽成活率,还对扦插生根、成活及移栽成活进行了讨论。展开更多
文摘The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.
