Flavonols possess significant medical value and are essential for plant stress resistance.These compounds constitute primary components of the nutritional value in onions,particularly in edible portions.While the flav...Flavonols possess significant medical value and are essential for plant stress resistance.These compounds constitute primary components of the nutritional value in onions,particularly in edible portions.While the flavonol biosynthetic pathway has been extensively studied,its regulatory mechanisms in onions remain incompletely understood.This investigation identified flavonol biosynthesis and regulatory genes through analysis of transcriptome and metabolomics data from different developmental stages of'SA1’.Two R2R3-MYB transcription factors,AcMYB12 and AcMYB29,were identified as positive regulators of onion flavonol biosynthesis.Transcriptional activation assays demonstrated that both could activate AcCHS,AcF3´H,and AcFLS.Yeast one-hybrid assays confirmed their direct binding to these gene promoters.The expression levels of flavonol pathway genes and flavonol contents in AcMYB12/AcMYB29-overexpressing onion calli and Arabidopsis plants were significantly higher than those in the control group.Transient silencing assays revealed partial functional redundancy between these two transcription factors.Notably,their regulatory capabilities exhibited significant differences.AcMYB12 predominantly regulates flavonol accumulation,while AcMYB29 specifically influences quercetin.Further investigation of the molecular mechanisms underlying differential regulation indicated variations in cis-elements within flavonol pathway gene promoters and differences in binding activity between transcription factors and cis-elements.展开更多
为了提高洋葱雄性不育恢复基因Ms位点的选择效率,加快育种进程,利用AcSKP1等位基因序列上的SNP差异位点,开发了适用于高通量检测的竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)标记。结果表明,KASP标记KMs4可以有效...为了提高洋葱雄性不育恢复基因Ms位点的选择效率,加快育种进程,利用AcSKP1等位基因序列上的SNP差异位点,开发了适用于高通量检测的竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)标记。结果表明,KASP标记KMs4可以有效地将F_(2)分离群体Ms位点的基因型分为MsMs、Msms、msms三类,并将BC_(1)分离群体Ms位点的基因型分为Msms和msms两类,其分型结果与AcSKP1标记鉴定及表型观察结果完全一致。此外,该标记在不同遗传背景已知基因型的材料中展现出良好的通用性。利用该标记对洋葱开放授粉群体进行育性基因型筛选系统评价,筛选出具有不同育性特征的育种系,为后续杂交选育提供了优质亲本材料。研究表明,KMs4标记是与洋葱雄性不育恢复基因Ms位点紧密连锁的分子标记,能够实现大规模育种材料的高效基因分型。展开更多
基金supported by the Key R&D Projects in Heilongjiang Province,China(GA21B012)the Collaborative Innovation Achievement Project of University in Heilongjiang Province,China(LJGXCG2022-040)。
文摘Flavonols possess significant medical value and are essential for plant stress resistance.These compounds constitute primary components of the nutritional value in onions,particularly in edible portions.While the flavonol biosynthetic pathway has been extensively studied,its regulatory mechanisms in onions remain incompletely understood.This investigation identified flavonol biosynthesis and regulatory genes through analysis of transcriptome and metabolomics data from different developmental stages of'SA1’.Two R2R3-MYB transcription factors,AcMYB12 and AcMYB29,were identified as positive regulators of onion flavonol biosynthesis.Transcriptional activation assays demonstrated that both could activate AcCHS,AcF3´H,and AcFLS.Yeast one-hybrid assays confirmed their direct binding to these gene promoters.The expression levels of flavonol pathway genes and flavonol contents in AcMYB12/AcMYB29-overexpressing onion calli and Arabidopsis plants were significantly higher than those in the control group.Transient silencing assays revealed partial functional redundancy between these two transcription factors.Notably,their regulatory capabilities exhibited significant differences.AcMYB12 predominantly regulates flavonol accumulation,while AcMYB29 specifically influences quercetin.Further investigation of the molecular mechanisms underlying differential regulation indicated variations in cis-elements within flavonol pathway gene promoters and differences in binding activity between transcription factors and cis-elements.
文摘为了提高洋葱雄性不育恢复基因Ms位点的选择效率,加快育种进程,利用AcSKP1等位基因序列上的SNP差异位点,开发了适用于高通量检测的竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)标记。结果表明,KASP标记KMs4可以有效地将F_(2)分离群体Ms位点的基因型分为MsMs、Msms、msms三类,并将BC_(1)分离群体Ms位点的基因型分为Msms和msms两类,其分型结果与AcSKP1标记鉴定及表型观察结果完全一致。此外,该标记在不同遗传背景已知基因型的材料中展现出良好的通用性。利用该标记对洋葱开放授粉群体进行育性基因型筛选系统评价,筛选出具有不同育性特征的育种系,为后续杂交选育提供了优质亲本材料。研究表明,KMs4标记是与洋葱雄性不育恢复基因Ms位点紧密连锁的分子标记,能够实现大规模育种材料的高效基因分型。
